pycom12g07700

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001605
Physical Location & Seq
Reverse (-)
8290662 .. 8292143
1482 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g07700.1

Sequence Viewer

Length: 1482 bp
ATGTCCAAAAAAAATGAACAAGTAGTTTCCATGAAGAAGGAGCTGTCCGTGACGAAAGTTACCAAAAGAGGAGGTGGACAAGCAAGCGGAGGAGACCAAGACCAACCTGCAGAAATCACCACCATGGAGCAAACTTCAGAAGTGAGAGTTACAATCCATGAGGAGGCAATAATAACCGGTTGGGGGAAGAAACTAAAAAAACTGAACGATGCATTGGCAGAAGCAGCTCAAAAAGAAAATGCCGTACCAAAGATCCAAAAGGTTCCCTTGTTGCTCCGTGATCACAAAAATTTCGACAACTACTACGAGCCAAGGGTATTAGCACTCGGTCCTTACCACCATGGTAACCGAAAGTACGAGCAGGCAGAGAAAGTCAAGCTTCAATTGGCAGCACACTTCATCACAGATGGGGATCATGGCCAGACTAGTTTCGGTGAAACTTTACTCAAGATGGTTGAGGACAACATTAAGGAGCTAAGGGAGTGCTATGATGAAGAAGCAACAAAGGACTACGGTGACGAGGTCCTCGCGTGGATGCTGTTCGTTGATGGGTGTTCGACGTTGGAATTCATATACAAATATAAAAATTTGGAAAAATTTAACATCAAGAGAGACCAAGTGGCATTTGCAGAACAGGACATGCTCTTGCTAGAGAATCAACTCCCCTATCGACTCCTCAAGTGGCTCTTCGACAAGAGCAAAATACGTGAGCAATTGAATAACTCAATCGAGGTTTTTGTCGAGATGCATGGTGGGGAACGAAAAGTTAATGACCATGCCGAGGAAAAAGAGCCACTTCATCTTCTTGACCTTCTTCGGACAAGAATGGTACGTCCACCTAGTACAAAACCAGGACCTCCACCTACAAAACCAGGAAGTAGCCATTCATTTCGCAATGTACAAGAGCTTCAGGCAGCCGGGATCTATTTTAGGTCTAGTGATGAAAACTCATTGAGGGCCATATCTTTTAAGTCTTGGTTTCAATGCTGTGGATTCCTTTATCTTCCTAAAATAAAAGTTGACGACTCGATGGGGCCTAAGTTCATGAATATGATAGCCTACGAAATGTGTCCTGATTTCCAGAATGATTTCGAGGTCACCTCTTACATAGGCTTCCTCGATTCGCTCATTGATCATCCAGACGATGTGAAGCACTTGAGGAAAAAGCGCATAGTTCAAAACTTGCTTGGGAGTGATGAGGAGGTGGCTCGACTCTTCAACGAGATAGGCACTGACTTGGTCCCTAACAATGCGATTTACCGCAATGTTACAGCACAGATAGAAGATCACTACAAAACCAAGTGGAAGACATGGATGACTCAATTCTTTCATGACCATTTCAGTAGTCCCTGGACTATCTTTGCTTTCTTTGGTGTTCTATTAGGACTCGGCTTGAGCGGTGCCCAGACGTGGTATGCAATCTTTTCCGATCCCTCTCCATGTGATGCCGTATGCAAGTACCTGAAAACACACCCCGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

494

Amino Acids

56.64

Weight (kDa)

6.18

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 85 - 463 7.5e-96 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 115
AccB1I GGYRCC 1 cut(s) 1400
AccBSI CCGCTC 1 cut(s) 1398
AccII CGCG 1 cut(s) 530
AciI CCGC 3 cut(s) 87, 1261, 1398
AclWI GGATC 4 cut(s) 247, 420, 929, 1424
AcoI YGGCCR 1 cut(s) 418
AcsI RAATTY 4 cut(s) 289, 566, 586, 596
AcuI CTGAAG 2 cut(s) 120, 893
AfaI GTAC 6 cut(s) 246, 356, 831, 844, 900, 1460
AfiI CCNNNNNNNGG 4 cut(s) 163, 183, 781, 1410
AgeI ACCGGT 1 cut(s) 176
AgsI TTSAA 5 cut(s) 383, 718, 983, 1178, 1219
AhlI ACTAGT 1 cut(s) 425
AjiI CACGTC 1 cut(s) 1410
AjnI CCWGG 3 cut(s) 850, 871, 1349
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 5 cut(s) 43, 227, 379, 475, 907
AluI AGCT 5 cut(s) 43, 227, 379, 475, 907
Alw26I GTCTC 2 cut(s) 87, 606
AlwI GGATC 4 cut(s) 247, 420, 929, 1424
AoxI GGCC 3 cut(s) 418, 957, 1034
ApeKI GCWGC 3 cut(s) 224, 389, 914
ApoI RAATTY 4 cut(s) 289, 566, 586, 596
AsiGI ACCGGT 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 1088
AspLEI GCGC 1 cut(s) 1170
AspS9I GGNCC 6 cut(s) 329, 523, 854, 957, 1034, 1240
AsuC2I CCSGG 2 cut(s) 919, 1476
AsuHPI GGTGA 4 cut(s) 109, 446, 527, 1090
AvaII GGWCC 4 cut(s) 329, 523, 854, 1240
BaeGI GKGCMC 1 cut(s) 1405
BalI TGGCCA 1 cut(s) 420
BanI GGYRCC 1 cut(s) 1400
BarI GAAGNNNNNNTAC 2 cut(s) 891, 923
BbsI GAAGAC 1 cut(s) 1313
BbvI GCAGC 3 cut(s) 236, 401, 926
BccI CCATC 4 cut(s) 401, 445, 542, 1024
BceAI ACGGC 2 cut(s) 227, 1433
BciT130I CCWGG 3 cut(s) 852, 873, 1351
BclI TGATCA 2 cut(s) 280, 1132
BcnI CCSGG 2 cut(s) 919, 1476
BcoDI GTCTC 2 cut(s) 87, 606
BcuI ACTAGT 1 cut(s) 425
BfaI CTAG 4 cut(s) 426, 650, 840, 936
BfmI CTRYAG 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 115
BisI GCNGC 3 cut(s) 225, 390, 915
BlsI GCNGC 3 cut(s) 226, 391, 916
Bme1390I CCNGG 5 cut(s) 852, 873, 919, 1351, 1476
Bme18I GGWCC 4 cut(s) 329, 523, 854, 1240
BmgBI CACGTC 1 cut(s) 1410
BmgT120I GGNCC 6 cut(s) 329, 523, 854, 957, 1034, 1240
BmiI GGNNCC 4 cut(s) 264, 1035, 1242, 1402
BmrFI CCNGG 5 cut(s) 852, 873, 919, 1351, 1476
BmsI GCATC 4 cut(s) 199, 525, 735, 1435
BpiI GAAGAC 1 cut(s) 1313
BplI GAGNNNNNCTC 2 cut(s) 1085, 1117
Bpu10I CCTNAGC 1 cut(s) 476
BpuEI CTTGAG 4 cut(s) 431, 662, 1177, 1414
BpuMI CCSGG 2 cut(s) 919, 1476
BsaAI YACGTR 1 cut(s) 707
BsaBI GATNNNNATC 1 cut(s) 411
BsaI GGTCTC 2 cut(s) 87, 606
BsaJI CCNNGG 6 cut(s) 123, 311, 340, 780, 1349, 1474
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 4 cut(s) 163, 183, 781, 1410
Bse118I RCCGGY 1 cut(s) 176
Bse3DI GCAATG 2 cut(s) 901, 1270
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 3 cut(s) 852, 873, 1351
BseDI CCNNGG 6 cut(s) 123, 311, 340, 780, 1349, 1474
BseGI GGATG 3 cut(s) 540, 1135, 1320
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 4 cut(s) 163, 183, 781, 1410
BseMI GCAATG 2 cut(s) 901, 1270
BseRI GAGGAG 5 cut(s) 84, 105, 176, 665, 1214
BseSI GKGCMC 1 cut(s) 1405
BseXI GCAGC 3 cut(s) 236, 401, 926
Bsh1236I CGCG 1 cut(s) 530
BshFI GGCC 3 cut(s) 420, 959, 1036
BshNI GGYRCC 1 cut(s) 1400
BshTI ACCGGT 1 cut(s) 176
BsiSI CCGG 3 cut(s) 177, 918, 1476
BslFI GGGAC 2 cut(s) 1226, 1332
BslI CCNNNNNNNGG 4 cut(s) 163, 183, 781, 1410
BsmAI GTCTC 2 cut(s) 87, 606
BsmFI GGGAC 2 cut(s) 1226, 1332
BsnI GGCC 3 cut(s) 420, 959, 1036
Bso31I GGTCTC 2 cut(s) 87, 606
Bsp1286I GDGCHC 1 cut(s) 1405
Bsp1407I TGTACA 1 cut(s) 898
Bsp143I GATC 7 cut(s) 252, 280, 412, 921, 1132, 1285, 1429
Bsp19I CCATGG 2 cut(s) 123, 340
BspACI CCGC 3 cut(s) 87, 1261, 1398
BspANI GGCC 3 cut(s) 420, 959, 1036
BspFNI CGCG 1 cut(s) 530
BspHI TCATGA 2 cut(s) 1044, 1330
BspLI GGNNCC 4 cut(s) 264, 1035, 1242, 1402
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 115
BspPI GGATC 4 cut(s) 247, 420, 929, 1424
BspQI GCTCTTC 1 cut(s) 692
BspT107I GGYRCC 1 cut(s) 1400
BspTNI GGTCTC 2 cut(s) 87, 606
BsrBI CCGCTC 1 cut(s) 1398
BsrDI GCAATG 2 cut(s) 901, 1270
BsrFI RCCGGY 1 cut(s) 176
BsrGI TGTACA 1 cut(s) 898
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 6 cut(s) 123, 311, 340, 780, 1349, 1474
BssMI GATC 7 cut(s) 252, 280, 412, 921, 1132, 1285, 1429
BssT1I CCWWGG 3 cut(s) 123, 311, 340
Bst2UI CCWGG 3 cut(s) 852, 873, 1351
Bst4CI ACNGT 1 cut(s) 515
Bst6I CTCTTC 2 cut(s) 692, 1220
BstAUI TGTACA 1 cut(s) 898
BstBAI YACGTR 1 cut(s) 707
BstC8I GCNNGC 2 cut(s) 85, 363
BstDEI CTNAG 2 cut(s) 476, 1038
BstDSI CCRYGG 2 cut(s) 123, 340
BstEII GGTNACC 2 cut(s) 344, 1096
BstF5I GGATG 3 cut(s) 540, 1135, 1320
BstFNI CGCG 1 cut(s) 530
BstHHI GCGC 1 cut(s) 1170
BstKTI GATC 7 cut(s) 255, 283, 415, 924, 1135, 1288, 1432
BstMAI GTCTC 2 cut(s) 87, 606
BstMBI GATC 7 cut(s) 252, 280, 412, 921, 1132, 1285, 1429
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNI CCWGG 3 cut(s) 852, 873, 1351
BstNSI RCATGY 1 cut(s) 643
BstPI GGTNACC 2 cut(s) 344, 1096
BstSCI CCNGG 5 cut(s) 850, 871, 917, 1349, 1474
BstSFI CTRYAG 1 cut(s) 108
BstSLI GKGCMC 1 cut(s) 1405
BstUI CGCG 1 cut(s) 530
BstV1I GCAGC 3 cut(s) 236, 401, 926
BstV2I GAAGAC 1 cut(s) 1313
BstX2I RGATCY 2 cut(s) 252, 921
BstYI RGATCY 2 cut(s) 252, 921
BsuRI GGCC 3 cut(s) 420, 959, 1036
BtgI CCRYGG 2 cut(s) 123, 340
BtrI CACGTC 1 cut(s) 1410
BtsCI GGATG 3 cut(s) 540, 1135, 1320
BtsIMutI CAGTG 1 cut(s) 1230
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 2 cut(s) 85, 363
CciI TCATGA 2 cut(s) 1044, 1330
CfoI GCGC 1 cut(s) 1170
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 6 cut(s) 329, 523, 854, 957, 1034, 1240
Csp6I GTAC 6 cut(s) 245, 355, 830, 843, 899, 1459
CspAI ACCGGT 1 cut(s) 176
CviQI GTAC 6 cut(s) 245, 355, 830, 843, 899, 1459
DdeI CTNAG 2 cut(s) 476, 1038
DpnI GATC 7 cut(s) 254, 282, 414, 923, 1134, 1287, 1431
DpnII GATC 7 cut(s) 252, 280, 412, 921, 1132, 1285, 1429
EaeI YGGCCR 1 cut(s) 418
Eam1104I CTCTTC 2 cut(s) 692, 1220
EarI CTCTTC 2 cut(s) 692, 1220
Eco130I CCWWGG 3 cut(s) 123, 311, 340
Eco31I GGTCTC 2 cut(s) 87, 606
Eco47I GGWCC 4 cut(s) 329, 523, 854, 1240
Eco57I CTGAAG 2 cut(s) 120, 893
Eco91I GGTNACC 2 cut(s) 344, 1096
EcoO109I RGGNCCY 3 cut(s) 523, 854, 1034
EcoO65I GGTNACC 2 cut(s) 344, 1096
EcoRI GAATTC 1 cut(s) 566
EcoRII CCWGG 3 cut(s) 850, 871, 1349
EcoT14I CCWWGG 3 cut(s) 123, 311, 340
EcoT22I ATGCAT 2 cut(s) 214, 750
ErhI CCWWGG 3 cut(s) 123, 311, 340
FalI AAGNNNNNCTT 8 cut(s) 251, 283, 363, 395, 671, 703, 780, 812
FaqI GGGAC 2 cut(s) 1226, 1332
FbaI TGATCA 2 cut(s) 280, 1132
Fnu4HI GCNGC 3 cut(s) 225, 390, 915
FokI GGATG 3 cut(s) 547, 1122, 1327
Fsp4HI GCNGC 3 cut(s) 225, 390, 915
FspBI CTAG 4 cut(s) 426, 650, 840, 936
GlaI GCGC 1 cut(s) 1169
GluI GCNGC 3 cut(s) 225, 390, 915
HaeIII GGCC 3 cut(s) 420, 959, 1036
HapII CCGG 3 cut(s) 177, 918, 1476
HhaI GCGC 1 cut(s) 1170
Hin6I GCGC 1 cut(s) 1168
HinP1I GCGC 1 cut(s) 1168
HincII GTYRAC 1 cut(s) 1021
HindII GTYRAC 1 cut(s) 1021
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 8 cut(s) 655, 672, 993, 1025, 1121, 1212, 1318, 1386
HpaII CCGG 3 cut(s) 177, 918, 1476
HphI GGTGA 4 cut(s) 109, 446, 527, 1090
Hpy166II GTNNAC 3 cut(s) 77, 836, 1021
Hpy188I TCNGA 3 cut(s) 139, 819, 1429
Hpy188III TCNNGA 9 cut(s) 448, 607, 742, 806, 1045, 1073, 1081, 1139, 1331
Hpy8I GTNNAC 3 cut(s) 77, 836, 1021
Hpy99I CGWCG 1 cut(s) 562
HpyAV CCTTC 2 cut(s) 31, 821
HpyCH4III ACNGT 1 cut(s) 515
HpyCH4IV ACGT 4 cut(s) 560, 706, 832, 1409
HpyCH4V TGCA 6 cut(s) 110, 212, 629, 748, 1418, 1455
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 2 cut(s) 476, 1038
HpySE526I ACGT 4 cut(s) 560, 706, 832, 1409
HspAI GCGC 1 cut(s) 1168
Ksp22I TGATCA 2 cut(s) 280, 1132
Kzo9I GATC 7 cut(s) 252, 280, 412, 921, 1132, 1285, 1429
LguI GCTCTTC 1 cut(s) 692
LmnI GCTCC 4 cut(s) 40, 127, 279, 472
Lsp1109I GCAGC 3 cut(s) 236, 401, 926
LweI GCATC 4 cut(s) 199, 525, 735, 1435
MaeI CTAG 4 cut(s) 426, 650, 840, 936
MaeII ACGT 4 cut(s) 560, 706, 832, 1409
MaeIII GTNAC 7 cut(s) 49, 58, 148, 344, 515, 1096, 1267
MalI GATC 7 cut(s) 254, 282, 414, 923, 1134, 1287, 1431
MbiI CCGCTC 1 cut(s) 1398
MboI GATC 7 cut(s) 252, 280, 412, 921, 1132, 1285, 1429
MfeI CAATTG 2 cut(s) 383, 713
MflI RGATCY 2 cut(s) 252, 921
MhlI GDGCHC 1 cut(s) 1405
MlsI TGGCCA 1 cut(s) 420
MluCI AATT 7 cut(s) 289, 383, 566, 586, 596, 713, 1322
MluNI TGGCCA 1 cut(s) 420
MlyI GAGTC 5 cut(s) 666, 1019, 1206, 1312, 1380
MmeI TCCRAC 1 cut(s) 543
Mox20I TGGCCA 1 cut(s) 420
Mph1103I ATGCAT 2 cut(s) 214, 750
MroXI GAANNNNTTC 1 cut(s) 1088
MscI TGGCCA 1 cut(s) 420
MseI TTAA 4 cut(s) 468, 600, 768, 969
MslI CAYNNNNRTG 2 cut(s) 122, 1049
Msp20I TGGCCA 1 cut(s) 420
MspI CCGG 3 cut(s) 177, 918, 1476
MspR9I CCNGG 5 cut(s) 852, 873, 919, 1351, 1476
MunI CAATTG 2 cut(s) 383, 713
MvaI CCWGG 3 cut(s) 852, 873, 1351
MvnI CGCG 1 cut(s) 530
MwoI GCNNNNNNNGC 1 cut(s) 224
NciI CCSGG 2 cut(s) 919, 1476
NcoI CCATGG 2 cut(s) 123, 340
NdeII GATC 7 cut(s) 252, 280, 412, 921, 1132, 1285, 1429
NlaIV GGNNCC 4 cut(s) 264, 1035, 1242, 1402
NmeAIII GCCGAG 2 cut(s) 805, 1368
NmuCI GTSAC 3 cut(s) 49, 515, 1096
NsiI ATGCAT 2 cut(s) 214, 750
NspI RCATGY 1 cut(s) 643
PagI TCATGA 2 cut(s) 1044, 1330
PciSI GCTCTTC 1 cut(s) 692
PdmI GAANNNNTTC 1 cut(s) 1088
PfeI GAWTC 3 cut(s) 655, 993, 1121
PflFI GACNNNGTC 2 cut(s) 521, 1238
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 3 cut(s) 226, 391, 916
PleI GAGTC 5 cut(s) 666, 1019, 1206, 1312, 1380
PpsI GAGTC 5 cut(s) 666, 1019, 1206, 1312, 1380
Ppu21I YACGTR 1 cut(s) 707
PpuMI RGGWCCY 2 cut(s) 523, 854
Psp5II RGGWCCY 2 cut(s) 523, 854
Psp6I CCWGG 3 cut(s) 850, 871, 1349
PspEI GGTNACC 2 cut(s) 344, 1096
PspGI CCWGG 3 cut(s) 850, 871, 1349
PspN4I GGNNCC 4 cut(s) 264, 1035, 1242, 1402
PspPI GGNCC 6 cut(s) 329, 523, 854, 957, 1034, 1240
PspPPI RGGWCCY 2 cut(s) 523, 854
PstI CTGCAG 1 cut(s) 112
PsuI RGATCY 2 cut(s) 252, 921
PsyI GACNNNGTC 2 cut(s) 521, 1238
RsaI GTAC 6 cut(s) 246, 356, 831, 844, 900, 1460
RsaNI GTAC 6 cut(s) 245, 355, 830, 843, 899, 1459
RseI CAYNNNNRTG 2 cut(s) 122, 1049
SapI GCTCTTC 1 cut(s) 692
SaqAI TTAA 4 cut(s) 468, 600, 768, 969
SatI GCNGC 3 cut(s) 225, 390, 915
Sau3AI GATC 7 cut(s) 252, 280, 412, 921, 1132, 1285, 1429
Sau96I GGNCC 6 cut(s) 329, 523, 854, 957, 1034, 1240
SchI GAGTC 5 cut(s) 666, 1019, 1206, 1312, 1380
ScrFI CCNGG 5 cut(s) 852, 873, 919, 1351, 1476
SduI GDGCHC 1 cut(s) 1405
SfaNI GCATC 4 cut(s) 199, 525, 735, 1435
SfcI CTRYAG 1 cut(s) 108
SinI GGWCC 4 cut(s) 329, 523, 854, 1240
SmiMI CAYNNNNRTG 2 cut(s) 122, 1049
SmlI CTYRAG 4 cut(s) 446, 677, 1156, 1393
SmoI CTYRAG 4 cut(s) 446, 677, 1156, 1393
SpeI ACTAGT 1 cut(s) 425
Sse9I AATT 7 cut(s) 289, 383, 566, 586, 596, 713, 1322
SsiI CCGC 3 cut(s) 87, 1261, 1398
SspMI CTAG 4 cut(s) 426, 650, 840, 936
StyD4I CCNGG 5 cut(s) 850, 871, 917, 1349, 1474
StyI CCWWGG 3 cut(s) 123, 311, 340
TaaI ACNGT 1 cut(s) 515
TaiI ACGT 4 cut(s) 563, 709, 835, 1412
TaqII GACCGA 1 cut(s) 317
TasI AATT 7 cut(s) 289, 383, 566, 586, 596, 713, 1322
TatI WGTACW 2 cut(s) 842, 898
TfiI GAWTC 3 cut(s) 655, 993, 1121
Tru1I TTAA 4 cut(s) 468, 600, 768, 969
Tru9I TTAA 4 cut(s) 468, 600, 768, 969
TscAI CASTG 1 cut(s) 1237
TseFI GTSAC 3 cut(s) 49, 515, 1096
TseI GCWGC 3 cut(s) 224, 389, 914
Tsp45I GTSAC 3 cut(s) 49, 515, 1096
TspGWI ACGGA 2 cut(s) 37, 266
TspRI CASTG 1 cut(s) 1237
Tth111I GACNNNGTC 2 cut(s) 521, 1238
VpaK11BI GGWCC 4 cut(s) 329, 523, 854, 1240
XapI RAATTY 4 cut(s) 289, 566, 586, 596
XceI RCATGY 1 cut(s) 643
XmnI GAANNNNTTC 1 cut(s) 1088
XspI CTAG 4 cut(s) 426, 650, 840, 936
Zsp2I ATGCAT 2 cut(s) 214, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.