MD12G1022100.v1.1

Plant protein of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001605
Physical Location & Seq
Reverse (-)
2193526 .. 2194863
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1022100.v1.1.491

Sequence Viewer

Length: 1338 bp
ATGGCTGATTCTTTGGAGCAGAGGTTTAATAGATTGAGGGATGCAGGAACAAAAAATCAGGATAGTTCTACAAGTGTTGTTCCAAGCAGGTCACGACCGATCATCCAAAATGTTTCCAGAACTCTCCGAAGTAATAAAAATTTGGAGAAATACTTTGAGCCAAGGGTACTAGCAATAGGCCCTATCCATCATGGAAAGTTTCCGGAATGGGAGGAGCTCAAATACAGATTGGCAGCGGACTTTGTCAATGACAGCGGCCGGAGTGAAGAATTTTTGTACAAGGAGGTTGAGGACAACATCAAGGAGTTAAAACAATGCTACGACGAGAAAACAACAGAGCGCTATGATGGTGATGAGGCGAGCCTCGCTTGGATGTTTTTCGTAGATGGTTGTTCAATCTTGCAGTTTATACAAAAGTATTCTACCTTGGAAGAGTTTGAGATAAAGAGAGACCAAGTGTTCTTTGCTGAACAAGACTTGTTCTTGCTAGAGAACCAAATCCCTTATGAAATCCTCAACCTTTTGATGAGCTCAAGCATAAAAAATGAGGACCTTTGGCACGGAATCCAGGCGTTTCTTGATATGCATAGTACTGCAGCTGGTGCATCAGCGAAGCCATTCAGATGGTATAAAAGGATGATGCGACAAGATCTGAAATTTGAATTGCGTTTGGGAGTATGTATGGAAGATTCATGGCCTACTACTCACCTTCTCGAGCTTCTTCGAACAAGAATGCTAGGTCCAGCAAAAAATGGCAGGGAGGCAGCTGATAATGAACTTCCACCATCATTTCGCAACGCGAAGGAGCTTATGGCAGCCGGGATCCATTTTAGGTGTAGTGAAGGTATGCTATTGCGTGACATATCTTTTAAATCACTATGCTTTGCAGGATTTCTCTTTCTTCCAGCAATGAACGCGGACGCAATGCCTTTGTTCTTGAATTTGATTGCCTACGAAATGTGTCCCGATTTCCAAAATGATTACGCGGTCACCTCTTACTTAAGCTTCCTGAGTTCATTCATTACTCATCCCGAAGATGCAAAGCAGCTAAGGTCAGCCCGCATACTTCACAATAAACTCGAAACTGACGAAGCACTCGCTCAACTCTTCAACGAGATAGGCCCTGATTTGGTATCAAACCCTTCGATAATTCACATCAGTAGCAGATTAGAAAAGCACTACAAATCCAAGTGGAAGGCATGGATGGCTCAGTTCTTTGAAGATCATTTCAGTAGCCCCTGGGCCATGCTTGCGTTCATCGGTGCTCTAACAGCACTTTTCTTGAGCGCCATCCAGACTTGGTACTCAATCATCAGTTATCATTATCCTAAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

446

Amino Acids

51.58

Weight (kDa)

5.72

Isoelectric Point (pI)

51.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 35 - 428 1.4e-77 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 78
AccII CGCG 3 cut(s) 800, 917, 986
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 5 cut(s) 236, 255, 917, 986, 1060
AclWI GGATC 2 cut(s) 817, 830
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 4 cut(s) 139, 269, 656, 940
AfaI GTAC 4 cut(s) 168, 278, 592, 1304
AfeI AGCGCT 1 cut(s) 341
AfiI CCNNNNNNNGG 1 cut(s) 1129
AflII CTTAAG 1 cut(s) 1000
AgsI TTSAA 5 cut(s) 396, 662, 940, 1111, 1220
AjnI CCWGG 2 cut(s) 567, 1238
AluBI AGCT 8 cut(s) 217, 531, 599, 718, 767, 808, 1005, 1048
AluI AGCT 8 cut(s) 217, 531, 599, 718, 767, 808, 1005, 1048
Alw21I GWGCWC 3 cut(s) 219, 533, 1267
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 2 cut(s) 817, 830
Ama87I CYCGRG 1 cut(s) 713
Aor13HI TCCGGA 1 cut(s) 202
Aor51HI AGCGCT 1 cut(s) 341
AoxI GGCC 5 cut(s) 178, 256, 695, 1120, 1242
ApeKI GCWGC 5 cut(s) 233, 596, 764, 815, 1045
ApoI RAATTY 4 cut(s) 139, 269, 656, 940
Asp700I GAANNNNTTC 1 cut(s) 617
AspLEI GCGC 2 cut(s) 342, 1289
AspS9I GGNCC 5 cut(s) 179, 550, 740, 1121, 1242
AsuC2I CCSGG 1 cut(s) 820
AsuHPI GGTGA 3 cut(s) 362, 698, 982
AsuII TTCGAA 1 cut(s) 724
AvaI CYCGRG 1 cut(s) 713
AvaII GGWCC 2 cut(s) 550, 740
BamHI GGATCC 1 cut(s) 822
BanII GRGCYC 2 cut(s) 219, 533
Bbv12I GWGCWC 3 cut(s) 219, 533, 1267
BbvI GCAGC 5 cut(s) 245, 608, 776, 827, 1057
BccI CCATC 7 cut(s) 195, 341, 380, 618, 793, 1198, 1298
BciT130I CCWGG 2 cut(s) 569, 1240
BcnI CCSGG 1 cut(s) 820
BcoDI GTCTC 1 cut(s) 444
BfaI CTAG 3 cut(s) 170, 488, 737
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 2 cut(s) 343, 1290
BfrI CTTAAG 1 cut(s) 1000
BfuAI ACCTGC 1 cut(s) 78
BglII AGATCT 1 cut(s) 649
BisI GCNGC 6 cut(s) 234, 256, 597, 765, 816, 1046
BlsI GCNGC 6 cut(s) 235, 257, 598, 766, 817, 1047
BmcAI AGTACT 1 cut(s) 592
Bme1390I CCNGG 3 cut(s) 569, 820, 1240
Bme18I GGWCC 2 cut(s) 550, 740
BmeT110I CYCGRG 1 cut(s) 713
BmgT120I GGNCC 5 cut(s) 179, 550, 740, 1121, 1242
BmiI GGNNCC 1 cut(s) 824
BmrFI CCNGG 3 cut(s) 569, 820, 1240
BmsI GCATC 4 cut(s) 31, 614, 630, 1027
Bpu10I CCTNAGC 1 cut(s) 1049
Bpu14I TTCGAA 1 cut(s) 724
BpuEI CTTGAG 2 cut(s) 517, 1303
BpuMI CCSGG 1 cut(s) 820
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 4 cut(s) 161, 426, 1238, 1239
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 1 cut(s) 1129
Bse3DI GCAATG 2 cut(s) 915, 930
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 2 cut(s) 569, 1240
BseDI CCNNGG 4 cut(s) 161, 426, 1238, 1239
BseGI GGATG 7 cut(s) 46, 102, 378, 642, 1027, 1209, 1290
BseLI CCNNNNNNNGG 1 cut(s) 1129
BseMI GCAATG 2 cut(s) 915, 930
BseMII CTCAG 2 cut(s) 1001, 1223
BseRI GAGGAG 1 cut(s) 227
BseX3I CGGCCG 1 cut(s) 256
BseXI GCAGC 5 cut(s) 245, 608, 776, 827, 1057
Bsh1236I CGCG 3 cut(s) 800, 917, 986
Bsh1285I CGRYCG 2 cut(s) 98, 259
BshFI GGCC 5 cut(s) 180, 258, 697, 1122, 1244
BsiEI CGRYCG 2 cut(s) 98, 259
BsiHKAI GWGCWC 3 cut(s) 219, 533, 1267
BsiHKCI CYCGRG 1 cut(s) 713
BsiSI CCGG 3 cut(s) 203, 259, 819
BslFI GGGAC 1 cut(s) 948
BslI CCNNNNNNNGG 1 cut(s) 1129
BsmAI GTCTC 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 948
BsmI GAATGC 1 cut(s) 738
BsnI GGCC 5 cut(s) 180, 258, 697, 1122, 1244
Bso31I GGTCTC 1 cut(s) 444
BsoBI CYCGRG 1 cut(s) 713
Bsp119I TTCGAA 1 cut(s) 724
Bsp1286I GDGCHC 3 cut(s) 219, 533, 1267
Bsp13I TCCGGA 1 cut(s) 202
Bsp1407I TGTACA 1 cut(s) 276
Bsp143I GATC 4 cut(s) 99, 649, 822, 1222
BspACI CCGC 5 cut(s) 236, 255, 917, 986, 1060
BspANI GGCC 5 cut(s) 180, 258, 697, 1122, 1244
BspCNI CTCAG 2 cut(s) 1002, 1222
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 3 cut(s) 800, 917, 986
BspLI GGNNCC 1 cut(s) 824
BspMAI CTGCAG 1 cut(s) 598
BspMI ACCTGC 1 cut(s) 78
BspPI GGATC 2 cut(s) 817, 830
BspT104I TTCGAA 1 cut(s) 724
BspTI CTTAAG 1 cut(s) 1000
BspTNI GGTCTC 1 cut(s) 444
BsrDI GCAATG 2 cut(s) 915, 930
BsrGI TGTACA 1 cut(s) 276
BssECI CCNNGG 4 cut(s) 161, 426, 1238, 1239
BssMI GATC 4 cut(s) 99, 649, 822, 1222
BssT1I CCWWGG 2 cut(s) 161, 426
Bst2UI CCWGG 2 cut(s) 569, 1240
Bst6I CTCTTC 2 cut(s) 426, 1112
BstAFI CTTAAG 1 cut(s) 1000
BstAPI GCANNNNNTGC 1 cut(s) 602
BstAUI TGTACA 1 cut(s) 276
BstBI TTCGAA 1 cut(s) 724
BstC8I GCNNGC 3 cut(s) 361, 1060, 1251
BstDEI CTNAG 4 cut(s) 1010, 1049, 1209, 1335
BstEII GGTNACC 1 cut(s) 988
BstF5I GGATG 7 cut(s) 46, 102, 378, 642, 1027, 1209, 1290
BstFNI CGCG 3 cut(s) 800, 917, 986
BstH2I RGCGCY 2 cut(s) 343, 1290
BstHHI GCGC 2 cut(s) 342, 1289
BstKTI GATC 4 cut(s) 102, 652, 825, 1225
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 4 cut(s) 99, 649, 822, 1222
BstMCI CGRYCG 2 cut(s) 98, 259
BstMWI GCNNNNNNNGC 6 cut(s) 365, 602, 914, 1205, 1250, 1271
BstNI CCWGG 2 cut(s) 569, 1240
BstPI GGTNACC 1 cut(s) 988
BstSCI CCNGG 3 cut(s) 567, 818, 1238
BstSFI CTRYAG 1 cut(s) 594
BstUI CGCG 3 cut(s) 800, 917, 986
BstV1I GCAGC 5 cut(s) 245, 608, 776, 827, 1057
BstX2I RGATCY 2 cut(s) 649, 822
BstXI CCANNNNNNTGG 1 cut(s) 624
BstYI RGATCY 2 cut(s) 649, 822
BstZI CGGCCG 1 cut(s) 256
BsuRI GGCC 5 cut(s) 180, 258, 697, 1122, 1244
BtsCI GGATG 7 cut(s) 46, 102, 378, 642, 1027, 1209, 1290
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 3 cut(s) 361, 1060, 1251
CfoI GCGC 2 cut(s) 342, 1289
Cfr13I GGNCC 5 cut(s) 179, 550, 740, 1121, 1242
CseI GACGC 1 cut(s) 929
Csp6I GTAC 4 cut(s) 167, 277, 591, 1303
CviAII CATG 4 cut(s) 191, 693, 1200, 1246
CviQI GTAC 4 cut(s) 167, 277, 591, 1303
DdeI CTNAG 4 cut(s) 1010, 1049, 1209, 1335
DpnI GATC 4 cut(s) 101, 651, 824, 1224
DpnII GATC 4 cut(s) 99, 649, 822, 1222
DraI TTTAAA 1 cut(s) 871
EaeI YGGCCR 1 cut(s) 256
EagI CGGCCG 1 cut(s) 256
Eam1104I CTCTTC 2 cut(s) 426, 1112
EarI CTCTTC 2 cut(s) 426, 1112
Ecl136II GAGCTC 2 cut(s) 217, 531
EclXI CGGCCG 1 cut(s) 256
Eco130I CCWWGG 2 cut(s) 161, 426
Eco24I GRGCYC 2 cut(s) 219, 533
Eco31I GGTCTC 1 cut(s) 444
Eco47I GGWCC 2 cut(s) 550, 740
Eco47III AGCGCT 1 cut(s) 341
Eco52I CGGCCG 1 cut(s) 256
Eco53kI GAGCTC 2 cut(s) 217, 531
Eco88I CYCGRG 1 cut(s) 713
Eco91I GGTNACC 1 cut(s) 988
EcoICRI GAGCTC 2 cut(s) 217, 531
EcoO109I RGGNCCY 3 cut(s) 179, 550, 1121
EcoO65I GGTNACC 1 cut(s) 988
EcoRII CCWGG 2 cut(s) 567, 1238
EcoT14I CCWWGG 2 cut(s) 161, 426
EcoT22I ATGCAT 1 cut(s) 588
EcoT38I GRGCYC 2 cut(s) 219, 533
ErhI CCWWGG 2 cut(s) 161, 426
FaeI CATG 4 cut(s) 194, 696, 1203, 1249
FaqI GGGAC 1 cut(s) 948
FatI CATG 4 cut(s) 190, 692, 1199, 1245
FauI CCCGC 1 cut(s) 1067
Fnu4HI GCNGC 6 cut(s) 234, 256, 597, 765, 816, 1046
FokI GGATG 7 cut(s) 53, 89, 385, 649, 1014, 1216, 1277
FriOI GRGCYC 2 cut(s) 219, 533
Fsp4HI GCNGC 6 cut(s) 234, 256, 597, 765, 816, 1046
FspBI CTAG 3 cut(s) 170, 488, 737
GlaI GCGC 2 cut(s) 341, 1288
GluI GCNGC 6 cut(s) 234, 256, 597, 765, 816, 1046
HaeII RGCGCY 2 cut(s) 343, 1290
HaeIII GGCC 5 cut(s) 180, 258, 697, 1122, 1244
HapII CCGG 3 cut(s) 203, 259, 819
HgaI GACGC 1 cut(s) 929
HhaI GCGC 2 cut(s) 342, 1289
Hin1II CATG 4 cut(s) 194, 696, 1203, 1249
Hin6I GCGC 2 cut(s) 340, 1287
HinP1I GCGC 2 cut(s) 340, 1287
HindIII AAGCTT 1 cut(s) 1003
HinfI GANTC 3 cut(s) 8, 564, 689
HpaII CCGG 3 cut(s) 203, 259, 819
HphI GGTGA 3 cut(s) 362, 698, 982
Hpy188I TCNGA 3 cut(s) 128, 623, 654
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 5 cut(s) 719, 796, 836, 1152, 1189
HpyCH4V TGCA 7 cut(s) 44, 403, 586, 596, 605, 887, 1040
HpyF10VI GCNNNNNNNGC 6 cut(s) 365, 602, 914, 1205, 1250, 1271
HpyF3I CTNAG 4 cut(s) 1010, 1049, 1209, 1335
Hsp92II CATG 4 cut(s) 194, 696, 1203, 1249
HspAI GCGC 2 cut(s) 340, 1287
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 4 cut(s) 99, 649, 822, 1222
LmnI GCTCC 3 cut(s) 16, 214, 805
Lsp1109I GCAGC 5 cut(s) 245, 608, 776, 827, 1057
LweI GCATC 4 cut(s) 31, 614, 630, 1027
MaeI CTAG 3 cut(s) 170, 488, 737
MaeIII GTNAC 3 cut(s) 90, 857, 988
MalI GATC 4 cut(s) 101, 651, 824, 1224
MboI GATC 4 cut(s) 99, 649, 822, 1222
MboII GAAGA 8 cut(s) 278, 443, 698, 713, 893, 1046, 1099, 1232
MflI RGATCY 2 cut(s) 649, 822
MhlI GDGCHC 3 cut(s) 219, 533, 1267
MluCI AATT 6 cut(s) 139, 269, 656, 662, 940, 1149
Mph1103I ATGCAT 1 cut(s) 588
MroI TCCGGA 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 617
MseI TTAA 4 cut(s) 27, 308, 870, 1001
MslI CAYNNNNRTG 1 cut(s) 622
MspA1I CMGCKG 4 cut(s) 236, 255, 599, 767
MspCI CTTAAG 1 cut(s) 1000
MspI CCGG 3 cut(s) 203, 259, 819
MspR9I CCNGG 3 cut(s) 569, 820, 1240
Mva1269I GAATGC 1 cut(s) 738
MvaI CCWGG 2 cut(s) 569, 1240
MvnI CGCG 3 cut(s) 800, 917, 986
MwoI GCNNNNNNNGC 6 cut(s) 365, 602, 914, 1205, 1250, 1271
NciI CCSGG 1 cut(s) 820
NdeII GATC 4 cut(s) 99, 649, 822, 1222
NlaIII CATG 4 cut(s) 194, 696, 1203, 1249
NlaIV GGNNCC 1 cut(s) 824
NmuCI GTSAC 3 cut(s) 90, 857, 988
NsiI ATGCAT 1 cut(s) 588
NspV TTCGAA 1 cut(s) 724
PaeR7I CTCGAG 1 cut(s) 713
PasI CCCWGGG 1 cut(s) 1239
PcsI WCGNNNNNNNCGW 1 cut(s) 1086
PctI GAATGC 1 cut(s) 738
PdmI GAANNNNTTC 1 cut(s) 617
PfeI GAWTC 3 cut(s) 8, 564, 689
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 6 cut(s) 235, 257, 598, 766, 817, 1047
PpuMI RGGWCCY 1 cut(s) 550
Psp124BI GAGCTC 2 cut(s) 219, 533
Psp5II RGGWCCY 1 cut(s) 550
Psp6I CCWGG 2 cut(s) 567, 1238
PspEI GGTNACC 1 cut(s) 988
PspGI CCWGG 2 cut(s) 567, 1238
PspN4I GGNNCC 1 cut(s) 824
PspPI GGNCC 5 cut(s) 179, 550, 740, 1121, 1242
PspPPI RGGWCCY 1 cut(s) 550
PstI CTGCAG 1 cut(s) 598
PsuI RGATCY 2 cut(s) 649, 822
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 2 cut(s) 599, 767
RsaI GTAC 4 cut(s) 168, 278, 592, 1304
RsaNI GTAC 4 cut(s) 167, 277, 591, 1303
RseI CAYNNNNRTG 1 cut(s) 622
SacI GAGCTC 2 cut(s) 219, 533
SaqAI TTAA 4 cut(s) 27, 308, 870, 1001
SatI GCNGC 6 cut(s) 234, 256, 597, 765, 816, 1046
Sau3AI GATC 4 cut(s) 99, 649, 822, 1222
Sau96I GGNCC 5 cut(s) 179, 550, 740, 1121, 1242
ScaI AGTACT 1 cut(s) 592
ScrFI CCNGG 3 cut(s) 569, 820, 1240
SduI GDGCHC 3 cut(s) 219, 533, 1267
SfaNI GCATC 4 cut(s) 31, 614, 630, 1027
SfcI CTRYAG 1 cut(s) 594
Sfr274I CTCGAG 1 cut(s) 713
SfuI TTCGAA 1 cut(s) 724
SinI GGWCC 2 cut(s) 550, 740
SlaI CTCGAG 1 cut(s) 713
SmiMI CAYNNNNRTG 1 cut(s) 622
SmlI CTYRAG 4 cut(s) 532, 713, 1000, 1282
SmoI CTYRAG 4 cut(s) 532, 713, 1000, 1282
Sse9I AATT 6 cut(s) 139, 269, 656, 662, 940, 1149
SsiI CCGC 5 cut(s) 236, 255, 917, 986, 1060
SspMI CTAG 3 cut(s) 170, 488, 737
SstI GAGCTC 2 cut(s) 219, 533
StyD4I CCNGG 3 cut(s) 567, 818, 1238
StyI CCWWGG 2 cut(s) 161, 426
TaqI TCGA 4 cut(s) 714, 724, 1080, 1145
TaqII GACCGA 1 cut(s) 112
TasI AATT 6 cut(s) 139, 269, 656, 662, 940, 1149
TatI WGTACW 2 cut(s) 276, 590
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 3 cut(s) 8, 564, 689
Tru1I TTAA 4 cut(s) 27, 308, 870, 1001
Tru9I TTAA 4 cut(s) 27, 308, 870, 1001
TseFI GTSAC 3 cut(s) 90, 857, 988
TseI GCWGC 5 cut(s) 233, 596, 764, 815, 1045
Tsp45I GTSAC 3 cut(s) 90, 857, 988
TspDTI ATGAA 7 cut(s) 522, 681, 789, 926, 1005, 1009, 1246
TspGWI ACGGA 1 cut(s) 576
Tth111I GACNNNGTC 1 cut(s) 242
Vha464I CTTAAG 1 cut(s) 1000
VpaK11BI GGWCC 2 cut(s) 550, 740
XapI RAATTY 4 cut(s) 139, 269, 656, 940
XhoI CTCGAG 1 cut(s) 713
XmnI GAANNNNTTC 1 cut(s) 617
XspI CTAG 3 cut(s) 170, 488, 737
ZrmI AGTACT 1 cut(s) 592
Zsp2I ATGCAT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.