Rw2G025360

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001605
Physical Location & Seq
Reverse (-)
37868224 .. 37869691
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G025360.1

Sequence Viewer

Length: 1227 bp
ATGGCTTCCAGGAATGAAGCTCCAAGTCAGAGCATCACAAACCATGAACAGTTATCTAAGGAGGAGTTGTTCATACACATACAGGAAGCAGGAGCGAGGCAATGTCAGTTTCAAAGCTCGAAACCAAAGATTCAAAGGGTTCCCAACATGCTCCGAGGTCACCCAAATTTCGAGAAATACTATGAGCCAAGGGCAGTGGCACTTGGTCCTATCCATCATGATAAACTTGCACTGGCAGCAAAATTTATCCAAGACAGTGGCCGTCCAGATAAATATTTGCACCAGATCGTTGAGGATAACATCAACACACTAAAGGACTGCTATGATGAGGAAGCAACAAGGTCCTACACCAACGAGGCCCTCACACACTTGTTATTCTTAGATGGATGTTCAACCCTACAGTTCATATACTCCTTTATGCACCATGAGTTAGCAAGTTTCAACATCAAGAGAGATCAGGTGGCTTTTGCAGAACAGGATTTGTTCTTGCTAGAAAACCAACTCCCTTACCAAGTCCTCAAGCTGTTGATGAGCTCAAGTGCCAAAGAGCAGAACTTGAAGGCCGCAATCGATGGATTTGTTGGAAGGAACATCATGGCAGCAGAAAAAAAATGGAAATGGCACAAACACGTTTGTCAACTGAGAGATGGTGTCATAGACATGGAAGAGCCCACTCATCTTCTCGAGCTTCTCCGGACAAAAATGCTAGGCTTTCATGGATTCCTAAATCTTCCACCACTATCTGTAGATGACTCCATGGGGCCCAAGTTCTTGAATTTGATAGCCTATGAGATGTGTCCTGATTTCCAAAATGACTCTGGGGTCACCTCTTACATATGCTTCCTGGATTCACTAATTGATCATGCAGATGATGTTAAACAGCTAAGGAAGGCACGCATACTACACAATTTACTTGGGAGTGATGAAGAGGTGGCTAAACTTTTCAATAAGATAGGTACTGACTTGGTGCCTAACTATGAAACATACTTCAGTGTCTGTCGCCAATTAGAAAAGCACTGCAATACCAAGTGGAAAACCAAGTTGATAGTCTGGATGGCTCAATTCTATCACGATCATTTCAGTAGCCCCTGGACTATCATGGCTTTACTGGGTGCTCTAGCAATTCTGGTGTTAACCGCCATCCAGACTTGGTACACAATAAATCCTCCGTCCACTGCCTGTGATGCTGTATGCGAGTACATGAAGAAACAGCTAAAAATAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

408

Amino Acids

46.94

Weight (kDa)

6.22

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 44 - 235 4.4e-28 Plant protein of unknown function
DUF247 PF03140 238 - 378 9.9e-43 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 821
AccB1I GGYRCC 1 cut(s) 967
AccIII TCCGGA 1 cut(s) 693
AciI CCGC 2 cut(s) 564, 1137
AcoI YGGCCR 1 cut(s) 259
AcsI RAATTY 3 cut(s) 166, 242, 775
AcuI CTGAAG 1 cut(s) 973
AfaI GTAC 3 cut(s) 958, 1154, 1199
AflIII ACRYGT 1 cut(s) 628
AgsI TTSAA 7 cut(s) 113, 134, 393, 442, 559, 775, 946
AjnI CCWGG 3 cut(s) 8, 843, 1088
AloI GAACNNNNNNTCC 2 cut(s) 53, 85
AluBI AGCT 7 cut(s) 20, 117, 523, 534, 688, 883, 1213
AluI AGCT 7 cut(s) 20, 117, 523, 534, 688, 883, 1213
Alw21I GWGCWC 2 cut(s) 536, 1117
AlwNI CAGNNNCTG 1 cut(s) 996
Ama87I CYCGRG 1 cut(s) 683
Aor13HI TCCGGA 1 cut(s) 693
AoxI GGCC 4 cut(s) 259, 357, 561, 761
ApaI GGGCCC 1 cut(s) 765
ApeKI GCWGC 2 cut(s) 236, 599
ApoI RAATTY 3 cut(s) 166, 242, 775
AspS9I GGNCC 5 cut(s) 206, 342, 358, 761, 762
AsuHPI GGTGA 2 cut(s) 152, 817
AvaI CYCGRG 1 cut(s) 683
AvaII GGWCC 2 cut(s) 206, 342
BaeGI GKGCMC 1 cut(s) 765
BanI GGYRCC 1 cut(s) 967
BanII GRGCYC 3 cut(s) 536, 672, 765
Bbv12I GWGCWC 2 cut(s) 536, 1117
BbvI GCAGC 2 cut(s) 248, 611
BccI CCATC 6 cut(s) 222, 377, 566, 641, 1048, 1148
BceAI ACGGC 1 cut(s) 246
BciT130I CCWGG 3 cut(s) 10, 845, 1090
BclI TGATCA 1 cut(s) 859
BfaI CTAG 3 cut(s) 491, 707, 1118
BfmI CTRYAG 2 cut(s) 398, 744
BisI GCNGC 3 cut(s) 237, 564, 600
BlsI GCNGC 3 cut(s) 238, 565, 601
Bme1390I CCNGG 3 cut(s) 10, 845, 1090
Bme18I GGWCC 2 cut(s) 206, 342
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 5 cut(s) 206, 342, 358, 761, 762
BmiI GGNNCC 4 cut(s) 141, 762, 763, 969
BmrFI CCNGG 3 cut(s) 10, 845, 1090
BmrI ACTGGG 1 cut(s) 1118
BmsI GCATC 2 cut(s) 42, 1174
BmuI ACTGGG 1 cut(s) 1118
Bpu10I CCTNAGC 1 cut(s) 884
BpuEI CTTGAG 2 cut(s) 503, 520
Bsa29I ATCGAT 1 cut(s) 570
BsaJI CCNNGG 4 cut(s) 154, 188, 756, 1088
BsaWI WCCGGW 1 cut(s) 693
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bse1I ACTGG 2 cut(s) 237, 1113
Bse3DI GCAATG 1 cut(s) 107
BseAI TCCGGA 1 cut(s) 693
BseBI CCWGG 3 cut(s) 10, 845, 1090
BseCI ATCGAT 1 cut(s) 570
BseDI CCNNGG 4 cut(s) 154, 188, 756, 1088
BseGI GGATG 3 cut(s) 392, 1059, 1140
BseMI GCAATG 1 cut(s) 107
BseMII CTCAG 1 cut(s) 632
BseNI ACTGG 2 cut(s) 237, 1113
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 765
BseXI GCAGC 2 cut(s) 248, 611
BshFI GGCC 4 cut(s) 261, 359, 563, 763
BshNI GGYRCC 1 cut(s) 967
BshVI ATCGAT 1 cut(s) 570
BsiHKAI GWGCWC 2 cut(s) 536, 1117
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 1 cut(s) 694
BsnI GGCC 4 cut(s) 261, 359, 563, 763
BsoBI CYCGRG 1 cut(s) 683
Bsp120I GGGCCC 1 cut(s) 761
Bsp1286I GDGCHC 4 cut(s) 536, 672, 765, 1117
Bsp13I TCCGGA 1 cut(s) 693
Bsp143I GATC 4 cut(s) 285, 454, 859, 1072
Bsp19I CCATGG 1 cut(s) 756
BspACI CCGC 2 cut(s) 564, 1137
BspANI GGCC 4 cut(s) 261, 359, 563, 763
BspCNI CTCAG 1 cut(s) 633
BspDI ATCGAT 1 cut(s) 570
BspEI TCCGGA 1 cut(s) 693
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 4 cut(s) 141, 762, 763, 969
BspQI GCTCTTC 1 cut(s) 660
BspT107I GGYRCC 1 cut(s) 967
BsrDI GCAATG 1 cut(s) 107
BsrI ACTGG 2 cut(s) 237, 1113
BssECI CCNNGG 4 cut(s) 154, 188, 756, 1088
BssMI GATC 4 cut(s) 285, 454, 859, 1072
BssT1I CCWWGG 2 cut(s) 188, 756
Bst2UI CCWGG 3 cut(s) 10, 845, 1090
Bst4CI ACNGT 3 cut(s) 51, 257, 402
Bst6I CTCTTC 2 cut(s) 660, 921
BstC8I GCNNGC 1 cut(s) 895
BstDEI CTNAG 4 cut(s) 57, 379, 641, 884
BstDSI CCRYGG 1 cut(s) 756
BstEII GGTNACC 2 cut(s) 158, 823
BstF5I GGATG 3 cut(s) 392, 1059, 1140
BstKTI GATC 4 cut(s) 288, 457, 862, 1075
BstMBI GATC 4 cut(s) 285, 454, 859, 1072
BstMWI GCNNNNNNNGC 2 cut(s) 236, 1184
BstNI CCWGG 3 cut(s) 10, 845, 1090
BstNSI RCATGY 1 cut(s) 151
BstPI GGTNACC 2 cut(s) 158, 823
BstSCI CCNGG 3 cut(s) 8, 843, 1088
BstSFI CTRYAG 2 cut(s) 398, 744
BstSLI GKGCMC 1 cut(s) 765
BstV1I GCAGC 2 cut(s) 248, 611
BstXI CCANNNNNNTGG 1 cut(s) 257
Bsu15I ATCGAT 1 cut(s) 570
BsuRI GGCC 4 cut(s) 261, 359, 563, 763
BsuTUI ATCGAT 1 cut(s) 570
BtgI CCRYGG 1 cut(s) 756
BtsCI GGATG 3 cut(s) 392, 1059, 1140
BtsI GCAGTG 3 cut(s) 201, 1015, 1173
BtsIMutI CAGTG 6 cut(s) 201, 230, 262, 997, 1015, 1173
Cac8I GCNNGC 1 cut(s) 895
CaiI CAGNNNCTG 1 cut(s) 996
CciI TCATGA 1 cut(s) 217
Cfr13I GGNCC 5 cut(s) 206, 342, 358, 761, 762
ClaI ATCGAT 1 cut(s) 570
Csp6I GTAC 3 cut(s) 957, 1153, 1198
CspCI CAANNNNNGTGG 2 cut(s) 177, 212
CviQI GTAC 3 cut(s) 957, 1153, 1198
DdeI CTNAG 4 cut(s) 57, 379, 641, 884
DpnI GATC 4 cut(s) 287, 456, 861, 1074
DpnII GATC 4 cut(s) 285, 454, 859, 1072
DrdI GACNNNNNNGTC 1 cut(s) 821
DseDI GACNNNNNNGTC 1 cut(s) 821
EaeI YGGCCR 1 cut(s) 259
Eam1104I CTCTTC 2 cut(s) 660, 921
EarI CTCTTC 2 cut(s) 660, 921
Ecl136II GAGCTC 1 cut(s) 534
Eco130I CCWWGG 2 cut(s) 188, 756
Eco24I GRGCYC 3 cut(s) 536, 672, 765
Eco47I GGWCC 2 cut(s) 206, 342
Eco53kI GAGCTC 1 cut(s) 534
Eco57I CTGAAG 1 cut(s) 973
Eco88I CYCGRG 1 cut(s) 683
Eco91I GGTNACC 2 cut(s) 158, 823
EcoICRI GAGCTC 1 cut(s) 534
EcoO109I RGGNCCY 3 cut(s) 342, 358, 761
EcoO65I GGTNACC 2 cut(s) 158, 823
EcoRII CCWGG 3 cut(s) 8, 843, 1088
EcoT14I CCWWGG 2 cut(s) 188, 756
EcoT38I GRGCYC 3 cut(s) 536, 672, 765
ErhI CCWWGG 2 cut(s) 188, 756
FauNDI CATATG 1 cut(s) 836
FbaI TGATCA 1 cut(s) 859
Fnu4HI GCNGC 3 cut(s) 237, 564, 600
FokI GGATG 3 cut(s) 399, 1066, 1127
FriOI GRGCYC 3 cut(s) 536, 672, 765
Fsp4HI GCNGC 3 cut(s) 237, 564, 600
FspBI CTAG 3 cut(s) 491, 707, 1118
GluI GCNGC 3 cut(s) 237, 564, 600
HaeIII GGCC 4 cut(s) 261, 359, 563, 763
HapII CCGG 1 cut(s) 694
HincII GTYRAC 2 cut(s) 638, 1134
HindII GTYRAC 2 cut(s) 638, 1134
HinfI GANTC 5 cut(s) 130, 720, 752, 815, 848
HpaI GTTAAC 1 cut(s) 1134
HpaII CCGG 1 cut(s) 694
HphI GGTGA 2 cut(s) 152, 817
Hpy166II GTNNAC 4 cut(s) 638, 1134, 1155, 1173
Hpy188I TCNGA 2 cut(s) 30, 155
Hpy8I GTNNAC 4 cut(s) 638, 1134, 1155, 1173
HpyAV CCTTC 3 cut(s) 553, 579, 883
HpyCH4III ACNGT 3 cut(s) 51, 257, 402
HpyCH4IV ACGT 1 cut(s) 630
HpyCH4V TGCA 6 cut(s) 230, 280, 421, 470, 866, 1020
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 1184
HpyF3I CTNAG 4 cut(s) 57, 379, 641, 884
HpySE526I ACGT 1 cut(s) 630
Kpn2I TCCGGA 1 cut(s) 693
Ksp22I TGATCA 1 cut(s) 859
KspAI GTTAAC 1 cut(s) 1134
Kzo9I GATC 4 cut(s) 285, 454, 859, 1072
LguI GCTCTTC 1 cut(s) 660
LmnI GCTCC 3 cut(s) 25, 92, 156
Lsp1109I GCAGC 2 cut(s) 248, 611
LweI GCATC 2 cut(s) 42, 1174
MaeI CTAG 3 cut(s) 491, 707, 1118
MaeII ACGT 1 cut(s) 630
MaeIII GTNAC 2 cut(s) 158, 823
MalI GATC 4 cut(s) 287, 456, 861, 1074
MboI GATC 4 cut(s) 285, 454, 859, 1072
MboII GAAGA 5 cut(s) 671, 677, 722, 938, 1216
MhlI GDGCHC 4 cut(s) 536, 672, 765, 1117
MluCI AATT 8 cut(s) 166, 242, 775, 855, 907, 1004, 1061, 1122
MlyI GAGTC 2 cut(s) 746, 809
MmeI TCCRAC 1 cut(s) 562
MroI TCCGGA 1 cut(s) 693
MseI TTAA 2 cut(s) 876, 1133
MslI CAYNNNNRTG 2 cut(s) 659, 867
MspI CCGG 1 cut(s) 694
MspR9I CCNGG 3 cut(s) 10, 845, 1090
MvaI CCWGG 3 cut(s) 10, 845, 1090
MwoI GCNNNNNNNGC 2 cut(s) 236, 1184
NcoI CCATGG 1 cut(s) 756
NdeI CATATG 1 cut(s) 836
NdeII GATC 4 cut(s) 285, 454, 859, 1072
NlaIV GGNNCC 4 cut(s) 141, 762, 763, 969
NmuCI GTSAC 2 cut(s) 158, 823
NspI RCATGY 1 cut(s) 151
PaeR7I CTCGAG 1 cut(s) 683
PagI TCATGA 1 cut(s) 217
PciSI GCTCTTC 1 cut(s) 660
PfeI GAWTC 3 cut(s) 130, 720, 848
PfoI TCCNGGA 2 cut(s) 8, 843
PkrI GCNGC 3 cut(s) 238, 565, 601
PleI GAGTC 2 cut(s) 746, 809
PpsI GAGTC 2 cut(s) 746, 809
PpuMI RGGWCCY 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 536
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 3 cut(s) 8, 843, 1088
PspEI GGTNACC 2 cut(s) 158, 823
PspGI CCWGG 3 cut(s) 8, 843, 1088
PspN4I GGNNCC 4 cut(s) 141, 762, 763, 969
PspOMI GGGCCC 1 cut(s) 761
PspPI GGNCC 5 cut(s) 206, 342, 358, 761, 762
PspPPI RGGWCCY 1 cut(s) 342
PstNI CAGNNNCTG 1 cut(s) 996
RsaI GTAC 3 cut(s) 958, 1154, 1199
RsaNI GTAC 3 cut(s) 957, 1153, 1198
RseI CAYNNNNRTG 2 cut(s) 659, 867
SacI GAGCTC 1 cut(s) 536
SapI GCTCTTC 1 cut(s) 660
SaqAI TTAA 2 cut(s) 876, 1133
SatI GCNGC 3 cut(s) 237, 564, 600
Sau3AI GATC 4 cut(s) 285, 454, 859, 1072
Sau96I GGNCC 5 cut(s) 206, 342, 358, 761, 762
SchI GAGTC 2 cut(s) 746, 809
ScrFI CCNGG 3 cut(s) 10, 845, 1090
SduI GDGCHC 4 cut(s) 536, 672, 765, 1117
SfaNI GCATC 2 cut(s) 42, 1174
SfcI CTRYAG 2 cut(s) 398, 744
Sfr274I CTCGAG 1 cut(s) 683
SinI GGWCC 2 cut(s) 206, 342
SlaI CTCGAG 1 cut(s) 683
SmiMI CAYNNNNRTG 2 cut(s) 659, 867
SmlI CTYRAG 3 cut(s) 518, 535, 683
SmoI CTYRAG 3 cut(s) 518, 535, 683
Sse9I AATT 8 cut(s) 166, 242, 775, 855, 907, 1004, 1061, 1122
SsiI CCGC 2 cut(s) 564, 1137
SspI AATATT 1 cut(s) 275
SspMI CTAG 3 cut(s) 491, 707, 1118
SstI GAGCTC 1 cut(s) 536
StyD4I CCNGG 3 cut(s) 8, 843, 1088
StyI CCWWGG 2 cut(s) 188, 756
TaaI ACNGT 3 cut(s) 51, 257, 402
TaiI ACGT 1 cut(s) 633
TaqI TCGA 4 cut(s) 119, 171, 570, 684
TasI AATT 8 cut(s) 166, 242, 775, 855, 907, 1004, 1061, 1122
TatI WGTACW 1 cut(s) 1197
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 3 cut(s) 130, 720, 848
Tru1I TTAA 2 cut(s) 876, 1133
Tru9I TTAA 2 cut(s) 876, 1133
TscAI CASTG 6 cut(s) 201, 237, 262, 997, 1022, 1180
TseFI GTSAC 2 cut(s) 158, 823
TseI GCWGC 2 cut(s) 236, 599
Tsp45I GTSAC 2 cut(s) 158, 823
TspDTI ATGAA 8 cut(s) 30, 60, 61, 394, 704, 939, 993, 1217
TspGWI ACGGA 1 cut(s) 1158
TspRI CASTG 6 cut(s) 201, 237, 262, 997, 1022, 1180
VpaK11BI GGWCC 2 cut(s) 206, 342
XapI RAATTY 3 cut(s) 166, 242, 775
XceI RCATGY 1 cut(s) 151
XcmI CCANNNNNNNNNTGG 1 cut(s) 815
XhoI CTCGAG 1 cut(s) 683
XspI CTAG 3 cut(s) 491, 707, 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.