Rh2BG321400

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001605
Physical Location & Seq
Reverse (-)
38687668 .. 38689068
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG321400.1

Sequence Viewer

Length: 1401 bp
ATGGCTTCCATGAATGAAGCTCCAAGTCGGAGCATCACAAGTCTTGAACAGTCATCTAAGGAGAAGTTGTTCAAAAACATAGAGGAAGCAGAAGCGAGGCTATGTCAGTTTCAAAGCTCGAAACCAAAGATTCAAAGGGTTCCCGACATGCTTCGAGATCACCCTAATCTCGAGAAATACTATGAGCCAAGGGCAGTGGCACTTGGTCCCTTCCATCATGATAAAGCAAGATGTCAGCTGGCAGAGAAGTTCAAGCTTGCACTGGCAGCAAAGTTTATCCAAGACAGTGGCCGTCCAGCTAAAGATTTGCACAAGATCGTTGAGGACAATATCAAGACACTAAAGGACTGCTATGATGAGGAAGCAACAATGTCCTACACCAACGAGGCCCTCACACACTTGTTATTCTTAGATGGGTGTTCAACCTTACAGTTCATATACTCATTTATGAATAACGAGTTGGCAAATTTCAAGATCAAGAGGGATCAGGTGGCTTTTGCAGAACAGGACTTGTTCCTGCTAGAAAACCAACTCCCTTACCAAGTCCTCAAGCTGTTGATGAGCTTAAGTGACAAAGAGATGGAATTGAAGACCGCAATCGATGGATTTGTTGGAAGGAACATCATGGCAGCAGGAAAAAAATGGAAATGGCACAAACACAAGAAAACGAGTCAACCAGCAGATATTGTCGTAAACATGGAAGAGCCCACTCATCTTCTCGAGCTTCTCAGGACAAAAATGCTAGGTCCCAAAGAATCTATCGACCCCAAGAAGAAAAAACCTAAAGATCGCCGACAATCATTCCGCAACATACAGGAGCTTAAAGCAACAGGGATCCATTTAAAGCCTAGTAAGACAAGCACCTTGAGGGACATATCTTTTACTCATCTTCTAGGCTTTGCAGGATTCCTATATCTTTCACCAATATCAGTAGACGACTCCACGAGGCCTAAGTTCTTGAATTTGATAGCCTATGAAATGTGTCCTGATTTCCAAAATGACTTTGGGGTCACCTCTTACATATGCTTCTTGGATTCACTGATTGATCATGCAGATGATGTTAAACAGCTAAGGTCGGCACGCGTACTACACAACTTACTTGGGAGTGATGAAGAGGTGGCTCAACTTTTCAATGAGATAGGCACTGACTTGGTGCCTAACAGTGAAACATACCGCAGTGTCAAATCCAAATTAGAAAAGCACTACAAAACTAAGTGGAAAAACTGGATGGCTCAATTCTGTCATGATCATTTCAGTAGCCCCTGGACTATCCTGGCTTTCCTTGGTGTTTTAGCAGCTCTGGGGTTAAGCAGCATCCAGACTTGGTACACAATCAATTCTCCCCCCTCTGCCTGCGATGCTGTATGCGAGTACTTGAAGAAACGGCTAAAAATAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

466

Amino Acids

53.49

Weight (kDa)

8.51

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 44 - 434 1.5e-92 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1007
AccB1I GGYRCC 1 cut(s) 1153
AccI GTMKAC 1 cut(s) 933
AccII CGCG 1 cut(s) 1083
AciI CCGC 3 cut(s) 594, 805, 1174
AclWI GGATC 3 cut(s) 492, 829, 842
AcoI YGGCCR 1 cut(s) 289
AcsI RAATTY 2 cut(s) 466, 961
AfaI GTAC 3 cut(s) 1086, 1328, 1373
AflII CTTAAG 1 cut(s) 565
AflIII ACRYGT 1 cut(s) 1081
AjnI CCWGG 2 cut(s) 1262, 1272
AjuI GAANNNNNNNTTGG 2 cut(s) 443, 475
AloI GAACNNNNNNTCC 2 cut(s) 53, 85
AlwI GGATC 3 cut(s) 492, 829, 842
Ama87I CYCGRG 2 cut(s) 170, 719
AoxI GGCC 3 cut(s) 289, 387, 947
ApeKI GCWGC 4 cut(s) 266, 629, 1295, 1311
ApoI RAATTY 2 cut(s) 466, 961
Asp700I GAANNNNTTC 1 cut(s) 68
AspS9I GGNCC 3 cut(s) 206, 388, 746
AsuHPI GGTGA 3 cut(s) 152, 912, 1003
AvaI CYCGRG 2 cut(s) 170, 719
AvaII GGWCC 2 cut(s) 206, 746
BamHI GGATCC 1 cut(s) 834
BanI GGYRCC 1 cut(s) 1153
BanII GRGCYC 1 cut(s) 708
BauI CACGAG 1 cut(s) 943
BbsI GAAGAC 1 cut(s) 596
BbvI GCAGC 4 cut(s) 278, 641, 1307, 1323
BccI CCATC 5 cut(s) 222, 407, 574, 596, 1222
BceAI ACGGC 1 cut(s) 276
BciT130I CCWGG 2 cut(s) 1264, 1274
BclI TGATCA 2 cut(s) 1045, 1246
BfaI CTAG 4 cut(s) 521, 743, 849, 893
BfrI CTTAAG 1 cut(s) 565
BisI GCNGC 4 cut(s) 267, 630, 1296, 1312
BlsI GCNGC 4 cut(s) 268, 631, 1297, 1313
BmcAI AGTACT 1 cut(s) 1373
Bme1390I CCNGG 2 cut(s) 1264, 1274
Bme18I GGWCC 2 cut(s) 206, 746
BmeT110I CYCGRG 2 cut(s) 170, 719
BmgT120I GGNCC 3 cut(s) 206, 388, 746
BmiI GGNNCC 5 cut(s) 141, 208, 748, 836, 1155
BmrFI CCNGG 2 cut(s) 1264, 1274
BmsI GCATC 3 cut(s) 42, 1323, 1348
BpiI GAAGAC 1 cut(s) 596
Bpu10I CCTNAGC 1 cut(s) 1070
BpuEI CTTGAG 2 cut(s) 533, 886
Bsa29I ATCGAT 1 cut(s) 600
BsaJI CCNNGG 3 cut(s) 188, 1262, 1282
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bse1I ACTGG 2 cut(s) 267, 1229
BseBI CCWGG 2 cut(s) 1264, 1274
BseCI ATCGAT 1 cut(s) 600
BseDI CCNNGG 3 cut(s) 188, 1262, 1282
BseGI GGATG 2 cut(s) 1233, 1314
BseMII CTCAG 1 cut(s) 742
BseNI ACTGG 2 cut(s) 267, 1229
BseXI GCAGC 4 cut(s) 278, 641, 1307, 1323
Bsh1236I CGCG 1 cut(s) 1083
BshFI GGCC 3 cut(s) 291, 389, 949
BshNI GGYRCC 1 cut(s) 1153
BshVI ATCGAT 1 cut(s) 600
BsiHKCI CYCGRG 2 cut(s) 170, 719
BslFI GGGAC 3 cut(s) 192, 732, 884
BsmFI GGGAC 3 cut(s) 192, 732, 884
BsnI GGCC 3 cut(s) 291, 389, 949
BsoBI CYCGRG 2 cut(s) 170, 719
Bsp1286I GDGCHC 1 cut(s) 708
Bsp143I GATC 8 cut(s) 157, 315, 474, 484, 787, 834, 1045, 1246
BspACI CCGC 3 cut(s) 594, 805, 1174
BspANI GGCC 3 cut(s) 291, 389, 949
BspCNI CTCAG 1 cut(s) 741
BspDI ATCGAT 1 cut(s) 600
BspFNI CGCG 1 cut(s) 1083
BspHI TCATGA 2 cut(s) 217, 1243
BspLI GGNNCC 5 cut(s) 141, 208, 748, 836, 1155
BspPI GGATC 3 cut(s) 492, 829, 842
BspQI GCTCTTC 1 cut(s) 696
BspT107I GGYRCC 1 cut(s) 1153
BspTI CTTAAG 1 cut(s) 565
BsrI ACTGG 2 cut(s) 267, 1229
BssECI CCNNGG 3 cut(s) 188, 1262, 1282
BssMI GATC 8 cut(s) 157, 315, 474, 484, 787, 834, 1045, 1246
BssSI CACGAG 1 cut(s) 943
BssT1I CCWWGG 2 cut(s) 188, 1282
Bst2BI CACGAG 1 cut(s) 943
Bst2UI CCWGG 2 cut(s) 1264, 1274
Bst4CI ACNGT 4 cut(s) 51, 287, 432, 1163
Bst6I CTCTTC 2 cut(s) 696, 1107
BstAFI CTTAAG 1 cut(s) 565
BstC8I GCNNGC 4 cut(s) 240, 258, 1081, 1354
BstDEI CTNAG 6 cut(s) 57, 409, 728, 951, 1070, 1212
BstEII GGTNACC 1 cut(s) 1009
BstF5I GGATG 2 cut(s) 1233, 1314
BstFNI CGCG 1 cut(s) 1083
BstKTI GATC 8 cut(s) 160, 318, 477, 487, 790, 837, 1048, 1249
BstMBI GATC 8 cut(s) 157, 315, 474, 484, 787, 834, 1045, 1246
BstMWI GCNNNNNNNGC 2 cut(s) 266, 1358
BstNI CCWGG 2 cut(s) 1264, 1274
BstNSI RCATGY 1 cut(s) 151
BstPI GGTNACC 1 cut(s) 1009
BstSCI CCNGG 2 cut(s) 1262, 1272
BstUI CGCG 1 cut(s) 1083
BstV1I GCAGC 4 cut(s) 278, 641, 1307, 1323
BstV2I GAAGAC 1 cut(s) 596
BstX2I RGATCY 1 cut(s) 834
BstXI CCANNNNNNTGG 1 cut(s) 287
BstYI RGATCY 1 cut(s) 834
Bsu15I ATCGAT 1 cut(s) 600
BsuRI GGCC 3 cut(s) 291, 389, 949
BsuTUI ATCGAT 1 cut(s) 600
BtgZI GCGATG 1 cut(s) 1371
BtsCI GGATG 2 cut(s) 1233, 1314
BtsI GCAGTG 2 cut(s) 201, 1183
BtsIMutI CAGTG 7 cut(s) 201, 260, 292, 1037, 1143, 1168, 1183
Cac8I GCNNGC 4 cut(s) 240, 258, 1081, 1354
CciI TCATGA 2 cut(s) 217, 1243
Cfr13I GGNCC 3 cut(s) 206, 388, 746
ClaI ATCGAT 1 cut(s) 600
Csp6I GTAC 3 cut(s) 1085, 1327, 1372
CspCI CAANNNNNGTGG 2 cut(s) 177, 212
CviAII CATG 7 cut(s) 10, 148, 218, 625, 697, 1049, 1244
CviQI GTAC 3 cut(s) 1085, 1327, 1372
DdeI CTNAG 6 cut(s) 57, 409, 728, 951, 1070, 1212
DpnI GATC 8 cut(s) 159, 317, 476, 486, 789, 836, 1047, 1248
DpnII GATC 8 cut(s) 157, 315, 474, 484, 787, 834, 1045, 1246
DraI TTTAAA 1 cut(s) 843
DrdI GACNNNNNNGTC 1 cut(s) 1007
DseDI GACNNNNNNGTC 1 cut(s) 1007
EaeI YGGCCR 1 cut(s) 289
Eam1104I CTCTTC 2 cut(s) 696, 1107
EarI CTCTTC 2 cut(s) 696, 1107
Eco130I CCWWGG 2 cut(s) 188, 1282
Eco147I AGGCCT 1 cut(s) 949
Eco24I GRGCYC 1 cut(s) 708
Eco47I GGWCC 2 cut(s) 206, 746
Eco88I CYCGRG 2 cut(s) 170, 719
Eco91I GGTNACC 1 cut(s) 1009
EcoO109I RGGNCCY 2 cut(s) 388, 746
EcoO65I GGTNACC 1 cut(s) 1009
EcoRII CCWGG 2 cut(s) 1262, 1272
EcoT14I CCWWGG 2 cut(s) 188, 1282
EcoT38I GRGCYC 1 cut(s) 708
ErhI CCWWGG 2 cut(s) 188, 1282
FaeI CATG 7 cut(s) 13, 151, 221, 628, 700, 1052, 1247
FaqI GGGAC 3 cut(s) 192, 732, 884
FatI CATG 7 cut(s) 9, 147, 217, 624, 696, 1048, 1243
FauNDI CATATG 1 cut(s) 1022
FbaI TGATCA 2 cut(s) 1045, 1246
FblI GTMKAC 1 cut(s) 933
Fnu4HI GCNGC 4 cut(s) 267, 630, 1296, 1312
FokI GGATG 2 cut(s) 1240, 1301
FriOI GRGCYC 1 cut(s) 708
Fsp4HI GCNGC 4 cut(s) 267, 630, 1296, 1312
FspBI CTAG 4 cut(s) 521, 743, 849, 893
GluI GCNGC 4 cut(s) 267, 630, 1296, 1312
HaeIII GGCC 3 cut(s) 291, 389, 949
Hin1II CATG 7 cut(s) 13, 151, 221, 628, 700, 1052, 1247
HincII GTYRAC 1 cut(s) 674
HindII GTYRAC 1 cut(s) 674
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 6 cut(s) 130, 670, 755, 906, 938, 1034
HphI GGTGA 3 cut(s) 152, 912, 1003
Hpy166II GTNNAC 4 cut(s) 674, 694, 934, 1329
Hpy188I TCNGA 1 cut(s) 30
Hpy8I GTNNAC 4 cut(s) 674, 694, 934, 1329
HpyAV CCTTC 2 cut(s) 220, 609
HpyCH4III ACNGT 4 cut(s) 51, 287, 432, 1163
HpyCH4V TGCA 5 cut(s) 260, 310, 500, 902, 1052
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 1358
HpyF3I CTNAG 6 cut(s) 57, 409, 728, 951, 1070, 1212
Hsp92II CATG 7 cut(s) 13, 151, 221, 628, 700, 1052, 1247
Ksp22I TGATCA 2 cut(s) 1045, 1246
Kzo9I GATC 8 cut(s) 157, 315, 474, 484, 787, 834, 1045, 1246
LguI GCTCTTC 1 cut(s) 696
LmnI GCTCC 3 cut(s) 25, 30, 817
Lsp1109I GCAGC 4 cut(s) 278, 641, 1307, 1323
LweI GCATC 3 cut(s) 42, 1323, 1348
MaeI CTAG 4 cut(s) 521, 743, 849, 893
MaeIII GTNAC 2 cut(s) 569, 1009
MalI GATC 8 cut(s) 159, 317, 476, 486, 789, 836, 1047, 1248
MboI GATC 8 cut(s) 157, 315, 474, 484, 787, 834, 1045, 1246
MboII GAAGA 7 cut(s) 601, 707, 713, 784, 881, 1124, 1390
MflI RGATCY 1 cut(s) 834
MhlI GDGCHC 1 cut(s) 708
MluCI AATT 6 cut(s) 466, 584, 961, 1190, 1235, 1336
MluI ACGCGT 1 cut(s) 1081
MlyI GAGTC 2 cut(s) 679, 932
MmeI TCCRAC 2 cut(s) 8, 592
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 5 cut(s) 566, 822, 842, 1062, 1307
MslI CAYNNNNRTG 1 cut(s) 1053
MspA1I CMGCKG 1 cut(s) 238
MspCI CTTAAG 1 cut(s) 565
MspR9I CCNGG 2 cut(s) 1264, 1274
MvaI CCWGG 2 cut(s) 1264, 1274
MvnI CGCG 1 cut(s) 1083
MwoI GCNNNNNNNGC 2 cut(s) 266, 1358
NdeI CATATG 1 cut(s) 1022
NdeII GATC 8 cut(s) 157, 315, 474, 484, 787, 834, 1045, 1246
NlaIII CATG 7 cut(s) 13, 151, 221, 628, 700, 1052, 1247
NlaIV GGNNCC 5 cut(s) 141, 208, 748, 836, 1155
NmuCI GTSAC 2 cut(s) 569, 1009
NspI RCATGY 1 cut(s) 151
PaeR7I CTCGAG 2 cut(s) 170, 719
PagI TCATGA 2 cut(s) 217, 1243
PceI AGGCCT 1 cut(s) 949
PciSI GCTCTTC 1 cut(s) 696
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 4 cut(s) 130, 755, 906, 1034
PkrI GCNGC 4 cut(s) 268, 631, 1297, 1313
PleI GAGTC 2 cut(s) 678, 932
PpsI GAGTC 2 cut(s) 678, 932
PpuMI RGGWCCY 1 cut(s) 746
Psp5II RGGWCCY 1 cut(s) 746
Psp6I CCWGG 2 cut(s) 1262, 1272
PspEI GGTNACC 1 cut(s) 1009
PspGI CCWGG 2 cut(s) 1262, 1272
PspN4I GGNNCC 5 cut(s) 141, 208, 748, 836, 1155
PspPI GGNCC 3 cut(s) 206, 388, 746
PspPPI RGGWCCY 1 cut(s) 746
PsuI RGATCY 1 cut(s) 834
PvuII CAGCTG 1 cut(s) 238
RsaI GTAC 3 cut(s) 1086, 1328, 1373
RsaNI GTAC 3 cut(s) 1085, 1327, 1372
RseI CAYNNNNRTG 1 cut(s) 1053
SapI GCTCTTC 1 cut(s) 696
SaqAI TTAA 5 cut(s) 566, 822, 842, 1062, 1307
SatI GCNGC 4 cut(s) 267, 630, 1296, 1312
Sau3AI GATC 8 cut(s) 157, 315, 474, 484, 787, 834, 1045, 1246
Sau96I GGNCC 3 cut(s) 206, 388, 746
ScaI AGTACT 1 cut(s) 1373
SchI GAGTC 2 cut(s) 679, 932
ScrFI CCNGG 2 cut(s) 1264, 1274
SduI GDGCHC 1 cut(s) 708
SfaNI GCATC 3 cut(s) 42, 1323, 1348
Sfr274I CTCGAG 2 cut(s) 170, 719
SinI GGWCC 2 cut(s) 206, 746
SlaI CTCGAG 2 cut(s) 170, 719
SmiMI CAYNNNNRTG 1 cut(s) 1053
SmlI CTYRAG 5 cut(s) 170, 548, 565, 719, 865
SmoI CTYRAG 5 cut(s) 170, 548, 565, 719, 865
Sse9I AATT 6 cut(s) 466, 584, 961, 1190, 1235, 1336
SseBI AGGCCT 1 cut(s) 949
SsiI CCGC 3 cut(s) 594, 805, 1174
SspMI CTAG 4 cut(s) 521, 743, 849, 893
StuI AGGCCT 1 cut(s) 949
StyD4I CCNGG 2 cut(s) 1262, 1272
StyI CCWWGG 2 cut(s) 188, 1282
TaaI ACNGT 4 cut(s) 51, 287, 432, 1163
TaqI TCGA 6 cut(s) 119, 154, 171, 600, 720, 762
TasI AATT 6 cut(s) 466, 584, 961, 1190, 1235, 1336
TatI WGTACW 1 cut(s) 1371
TfiI GAWTC 4 cut(s) 130, 755, 906, 1034
Tru1I TTAA 5 cut(s) 566, 822, 842, 1062, 1307
Tru9I TTAA 5 cut(s) 566, 822, 842, 1062, 1307
TscAI CASTG 7 cut(s) 201, 267, 292, 1044, 1150, 1168, 1183
TseFI GTSAC 2 cut(s) 569, 1009
TseI GCWGC 4 cut(s) 266, 629, 1295, 1311
Tsp45I GTSAC 2 cut(s) 569, 1009
TspDTI ATGAA 6 cut(s) 26, 30, 424, 464, 990, 1125
TspRI CASTG 7 cut(s) 201, 267, 292, 1044, 1150, 1168, 1183
Vha464I CTTAAG 1 cut(s) 565
VpaK11BI GGWCC 2 cut(s) 206, 746
XapI RAATTY 2 cut(s) 466, 961
XceI RCATGY 1 cut(s) 151
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XhoI CTCGAG 2 cut(s) 170, 719
XmiI GTMKAC 1 cut(s) 933
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 4 cut(s) 521, 743, 849, 893
ZrmI AGTACT 1 cut(s) 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.