MD04G1194400.v1.1

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000631
Physical Location & Seq
Reverse (-)
28370388 .. 28371346
959 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1194400.v1.1.491

Sequence Viewer

Length: 822 bp
ATGCTCTCACACAGTCCTACACTCCACATCTCTACGATTTCTGAAACAATTTCTCACTCATTGATCATTCTACTTAATTGCTTCACTAGTGAGAGCATGGTGAAAAAGGCTAGTTTTCCTAGAAAAGGAGTTTTTCAAAAAGGTGACAAGGTGGAAGTGTGCAGCAAAGAATATGGATTTATCGGCTCTTATTATGCAGCAATTGTACTTGAAAAGTTGGGGGACAAGTACAAGGTGGTGTACAAGAACCTGGTGGAGGAGGACGAGCTGTCCAAACCTCTAGTAGAGATTGTCACAGCAGATGAGGTCCGTCCTCGTCCTTCTCCACCTCCCCAAGAAATCGTGGATTTAGAGTTTGATGTCCTTGATAGAGTTGATGTATTTGACAACAAGGGTTGGTGGGTAGGCACCATTACTGAGAAGAGAGGGGGGGACAAATACTTCGTCTACTTTGAAACGACTGCGGATCATATAGCTTACCCTAAGAGGAAACTGAGGTTTCATAGGGACTGGATCGATGGACAGTGGCTGGTCTACAACAAGAAGAAGAGAGTTGTTCAGGTAGACGGAAAGTTGGTGGATTGCAAGAAGAGAGTCCGCGCGCAGGTTGATGAAAGAGTTGTGGACTGCTACAAGAGAGTGCGCATCGATGGAAACTTGGTGGATTGCAAGGACAGGTTAAGAGTCAGCGTTGTACCTTTTTCCACTCTGATTAATTTCTTGGTGTATGGTGGAAACAATTTGGGAATATTGCTATTTAAGTGCAATTTATCAGGGTTTTCAGTAATTTCCTTTCATTTTTATTTATTATTATTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

274

Amino Acids

31.45

Weight (kDa)

8.86

Isoelectric Point (pI)

28.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 48 - 107 2.5e-17 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06340
fragaria_vesca FvH4_2g23230 FvH4_6g05270 FvH4_6g14470
malus_domestica MD04G1194400.v1.1 MD04G1194500.v1.1 MD04G1194700.v1.1 MD04G1194900.v1.1 MD04G1199300.v1.1 MD12G1207100.v1.1
prunus_persica Prupe.6G295700_v2.0.a1 Prupe.6G314400_v2.0.a1 Prupe.6G314500_v2.0.a1
pyrus_communis pycom04g17200 pycom12g19490
rosa_chinensis RchiOBHm_Chr1g0336721 RchiOBHm_Chr3g0454011 RchiOBHm_Chr3g0484961 RchiOBHm_Chr6g0290501 RchiOBHm_Chr6g0290521 RchiOBHm_Chr6g0290541 RchiOBHm_Chr7g0235761
rosa_laevigata RLG00000001142 RLG00000006528 RLG00000012205 RLG00000012207 RLG00000012209 RLG00000024490 RLG00000025490 RLG00000035862
rosa_multiflora Rmu_co8053086.1_g000001 Rmu_sc0000679.1_g000031 Rmu_sc0001089.1_g000020 Rmu_sc0001302.1_g000001 Rmu_sc0002477.1_g000009 Rmu_sc0002724.1_g000039 Rmu_sc0002890.1_g000020 Rmu_sc0002890.1_g000022 Rmu_sc0003556.1_g000036 Rmu_sc0009295.1_g000012 Rmu_sc0011632.1_g000017 Rmu_sc0019265.1_g000002
rosa_roxburghii Rroxscaffold_4G00326260 Rroxscaffold_6G00413590 Rroxscaffold_7G00176960 Rroxscaffold_7G00176980 Rroxscaffold_7G00177000
rosa_rugosa Rorug06G0212900
rosa_samantha Rh1AG103800 Rh1BG086300 Rh2AG280500 Rh2AG280600 Rh3AG054900 Rh3AG136100 Rh3BG056500 Rh3BG158000 Rh3CG055700 Rh3CG157800 Rh3DG057000 Rh3DG158600 Rh6AG323500 Rh6AG323700 Rh6BG330700 Rh6BG330900 Rh6BG331100 Rh6BG413200 Rh6CG337000 Rh6CG337100 Rh6CG337300 Rh6DG323900 Rh6DG324100 Rh6DG324300 Rh7DG436300
rosa_wichuraiana Rw3G004220 Rw3G012920 Rw6G028060 Rw6G028080 Rw7G037160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 644
Acc36I ACCTGC 1 cut(s) 595
AccB1I GGYRCC 1 cut(s) 407
AccI GTMKAC 3 cut(s) 447, 534, 564
AccII CGCG 2 cut(s) 600, 602
AciI CCGC 2 cut(s) 464, 598
AclWI GGATC 2 cut(s) 474, 521
AfaI GTAC 4 cut(s) 207, 230, 242, 696
AfiI CCNNNNNNNGG 3 cut(s) 125, 256, 604
AgsI TTSAA 3 cut(s) 137, 212, 455
AhdI GACNNNNNGTC 1 cut(s) 268
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 1 cut(s) 249
AluBI AGCT 2 cut(s) 268, 476
AluI AGCT 2 cut(s) 268, 476
AlwI GGATC 2 cut(s) 474, 521
AlwNI CAGNNNCTG 1 cut(s) 529
ApeKI GCWGC 2 cut(s) 162, 197
ArsI GACNNNNNNTTYG 4 cut(s) 425, 429, 457, 461
AseI ATTAAT 1 cut(s) 714
AspLEI GCGC 3 cut(s) 602, 604, 645
AspS9I GGNCC 1 cut(s) 307
AsuHPI GGTGA 2 cut(s) 112, 155
AvaII GGWCC 1 cut(s) 307
BanI GGYRCC 1 cut(s) 407
BbvI GCAGC 2 cut(s) 174, 209
BccI CCATC 2 cut(s) 512, 644
BciT130I CCWGG 1 cut(s) 251
BclI TGATCA 1 cut(s) 63
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 4 cut(s) 87, 111, 120, 281
BfuAI ACCTGC 1 cut(s) 595
BisI GCNGC 2 cut(s) 163, 198
BlsI GCNGC 2 cut(s) 164, 199
Bme1390I CCNGG 1 cut(s) 251
Bme18I GGWCC 1 cut(s) 307
BmeRI GACNNNNNGTC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 1 cut(s) 409
BmrFI CCNGG 1 cut(s) 251
BmsI GCATC 1 cut(s) 654
Bsa29I ATCGAT 2 cut(s) 516, 648
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 256, 604
Bse1I ACTGG 1 cut(s) 515
BseBI CCWGG 1 cut(s) 251
BseCI ATCGAT 2 cut(s) 516, 648
BseLI CCNNNNNNNGG 3 cut(s) 125, 256, 604
BseMII CTCAG 2 cut(s) 408, 485
BseNI ACTGG 1 cut(s) 515
BsePI GCGCGC 1 cut(s) 600
BseRI GAGGAG 1 cut(s) 272
BseXI GCAGC 2 cut(s) 174, 209
BsgI GTGCAG 1 cut(s) 181
Bsh1236I CGCG 2 cut(s) 600, 602
BshNI GGYRCC 1 cut(s) 407
BshVI ATCGAT 2 cut(s) 516, 648
BslFI GGGAC 3 cut(s) 236, 446, 521
BslI CCNNNNNNNGG 3 cut(s) 125, 256, 604
BsmFI GGGAC 3 cut(s) 236, 446, 521
Bsp1407I TGTACA 1 cut(s) 240
Bsp143I GATC 3 cut(s) 63, 466, 513
BspACI CCGC 2 cut(s) 464, 598
BspCNI CTCAG 2 cut(s) 409, 486
BspDI ATCGAT 2 cut(s) 516, 648
BspFNI CGCG 2 cut(s) 600, 602
BspLI GGNNCC 1 cut(s) 409
BspMI ACCTGC 1 cut(s) 595
BspPI GGATC 2 cut(s) 474, 521
BspT107I GGYRCC 1 cut(s) 407
BsrGI TGTACA 1 cut(s) 240
BsrI ACTGG 1 cut(s) 515
BssHII GCGCGC 1 cut(s) 600
BssMI GATC 3 cut(s) 63, 466, 513
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 2 cut(s) 14, 525
Bst6I CTCTTC 3 cut(s) 416, 542, 584
BstAUI TGTACA 1 cut(s) 240
BstC8I GCNNGC 1 cut(s) 602
BstDEI CTNAG 3 cut(s) 417, 483, 494
BstENI CCTNNNNNAGG 2 cut(s) 123, 254
BstFNI CGCG 2 cut(s) 600, 602
BstHHI GCGC 3 cut(s) 602, 604, 645
BstKTI GATC 3 cut(s) 66, 469, 516
BstMBI GATC 3 cut(s) 63, 466, 513
BstNI CCWGG 1 cut(s) 251
BstSCI CCNGG 1 cut(s) 249
BstUI CGCG 2 cut(s) 600, 602
BstV1I GCAGC 2 cut(s) 174, 209
Bsu15I ATCGAT 2 cut(s) 516, 648
BsuTUI ATCGAT 2 cut(s) 516, 648
BtsIMutI CAGTG 1 cut(s) 530
BveI ACCTGC 1 cut(s) 595
Cac8I GCNNGC 1 cut(s) 602
CaiI CAGNNNCTG 1 cut(s) 529
CfoI GCGC 3 cut(s) 602, 604, 645
Cfr13I GGNCC 1 cut(s) 307
ClaI ATCGAT 2 cut(s) 516, 648
CsiI ACCWGGT 1 cut(s) 249
Csp6I GTAC 4 cut(s) 206, 229, 241, 695
CviAII CATG 1 cut(s) 97
CviJI RGCY 5 cut(s) 110, 186, 268, 476, 529
CviKI_1 RGCY 5 cut(s) 110, 186, 268, 476, 529
CviQI GTAC 4 cut(s) 206, 229, 241, 695
DdeI CTNAG 3 cut(s) 417, 483, 494
DpnI GATC 3 cut(s) 65, 468, 515
DpnII GATC 3 cut(s) 63, 466, 513
DriI GACNNNNNGTC 1 cut(s) 268
Eam1104I CTCTTC 3 cut(s) 416, 542, 584
Eam1105I GACNNNNNGTC 1 cut(s) 268
EarI CTCTTC 3 cut(s) 416, 542, 584
Eco47I GGWCC 1 cut(s) 307
EcoNI CCTNNNNNAGG 2 cut(s) 123, 254
EcoRII CCWGG 1 cut(s) 249
FaeI CATG 1 cut(s) 100
FaiI YATR 8 cut(s) 98, 174, 195, 471, 473, 504, 729, 820
FaqI GGGAC 3 cut(s) 236, 446, 521
FatI CATG 1 cut(s) 96
FbaI TGATCA 1 cut(s) 63
FblI GTMKAC 3 cut(s) 447, 534, 564
Fnu4HI GCNGC 2 cut(s) 163, 198
Fsp4HI GCNGC 2 cut(s) 163, 198
FspAI RTGCGCAY 1 cut(s) 644
FspBI CTAG 4 cut(s) 87, 111, 120, 281
FspI TGCGCA 1 cut(s) 644
GlaI GCGC 3 cut(s) 601, 603, 644
GluI GCNGC 2 cut(s) 163, 198
HhaI GCGC 3 cut(s) 602, 604, 645
Hin1II CATG 1 cut(s) 100
Hin6I GCGC 3 cut(s) 600, 602, 643
HinP1I GCGC 3 cut(s) 600, 602, 643
HinfI GANTC 2 cut(s) 594, 684
HphI GGTGA 2 cut(s) 112, 155
Hpy166II GTNNAC 5 cut(s) 241, 448, 535, 565, 625
Hpy188I TCNGA 2 cut(s) 43, 711
Hpy8I GTNNAC 5 cut(s) 241, 448, 535, 565, 625
HpyAV CCTTC 1 cut(s) 330
HpyCH4III ACNGT 2 cut(s) 14, 525
HpyCH4V TGCA 5 cut(s) 162, 197, 585, 669, 765
HpyF3I CTNAG 3 cut(s) 417, 483, 494
Hsp92II CATG 1 cut(s) 100
HspAI GCGC 3 cut(s) 600, 602, 643
Ksp22I TGATCA 1 cut(s) 63
Kzo9I GATC 3 cut(s) 63, 466, 513
LpnPI CCDG 8 cut(s) 236, 263, 496, 515, 545, 590, 661, 759
Lsp1109I GCAGC 2 cut(s) 174, 209
LweI GCATC 1 cut(s) 654
MabI ACCWGGT 1 cut(s) 249
MaeI CTAG 4 cut(s) 87, 111, 120, 281
MaeIII GTNAC 2 cut(s) 143, 292
MalI GATC 3 cut(s) 65, 468, 515
MboI GATC 3 cut(s) 63, 466, 513
MboII GAAGA 4 cut(s) 433, 556, 559, 601
MfeI CAATTG 1 cut(s) 201
MluCI AATT 7 cut(s) 48, 76, 201, 715, 739, 766, 786
MlyI GAGTC 2 cut(s) 603, 693
MnlI CCTC 9 cut(s) 250, 253, 288, 298, 324, 339, 419, 480, 489
MseI TTAA 4 cut(s) 75, 680, 714, 759
MspR9I CCNGG 1 cut(s) 251
MunI CAATTG 1 cut(s) 201
MvaI CCWGG 1 cut(s) 251
MvnI CGCG 2 cut(s) 600, 602
NdeII GATC 3 cut(s) 63, 466, 513
NlaIII CATG 1 cut(s) 100
NlaIV GGNNCC 1 cut(s) 409
NmuCI GTSAC 2 cut(s) 143, 292
NsbI TGCGCA 1 cut(s) 644
PauI GCGCGC 1 cut(s) 600
PkrI GCNGC 2 cut(s) 164, 199
PleI GAGTC 2 cut(s) 602, 692
PpsI GAGTC 2 cut(s) 602, 692
PshBI ATTAAT 1 cut(s) 714
Psp6I CCWGG 1 cut(s) 249
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 1 cut(s) 409
PspPI GGNCC 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 529
PteI GCGCGC 1 cut(s) 600
RsaI GTAC 4 cut(s) 207, 230, 242, 696
RsaNI GTAC 4 cut(s) 206, 229, 241, 695
SaqAI TTAA 4 cut(s) 75, 680, 714, 759
SatI GCNGC 2 cut(s) 163, 198
Sau3AI GATC 3 cut(s) 63, 466, 513
Sau96I GGNCC 1 cut(s) 307
SchI GAGTC 2 cut(s) 603, 693
ScrFI CCNGG 1 cut(s) 251
SexAI ACCWGGT 1 cut(s) 249
SfaNI GCATC 1 cut(s) 654
SinI GGWCC 1 cut(s) 307
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 7 cut(s) 48, 76, 201, 715, 739, 766, 786
SsiI CCGC 2 cut(s) 464, 598
SspI AATATT 1 cut(s) 750
SspMI CTAG 4 cut(s) 87, 111, 120, 281
StyD4I CCNGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 14, 525
TaqI TCGA 2 cut(s) 516, 648
TasI AATT 7 cut(s) 48, 76, 201, 715, 739, 766, 786
TatI WGTACW 3 cut(s) 205, 228, 240
Tru1I TTAA 4 cut(s) 75, 680, 714, 759
Tru9I TTAA 4 cut(s) 75, 680, 714, 759
TscAI CASTG 1 cut(s) 530
TseFI GTSAC 2 cut(s) 143, 292
TseI GCWGC 2 cut(s) 162, 197
Tsp45I GTSAC 2 cut(s) 143, 292
TspDTI ATGAA 3 cut(s) 491, 627, 785
TspGWI ACGGA 2 cut(s) 299, 582
TspRI CASTG 1 cut(s) 530
VpaK11BI GGWCC 1 cut(s) 307
VspI ATTAAT 1 cut(s) 714
XagI CCTNNNNNAGG 2 cut(s) 123, 254
XmiI GTMKAC 3 cut(s) 447, 534, 564
XspI CTAG 4 cut(s) 87, 111, 120, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.