MD12G1207100.v1.1

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000631
Physical Location & Seq
Forward (+)
28720137 .. 28720763
627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1207100.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGGGCCTTCCAGTGCGATCCAACGTCGTAAAAACACGACGAACTCTCGTCCTTCTGCTTCCTCATCTCACTCCCAACCCTCCGATCCGACTGAGTTCTGCCCCGAAAGGTGGTTCGAAACAAACAGCGAGGTTGAAGTGTTAAGCAAGGAGGAGGGGTTCATCGGATCGTACTTCGCAGCTAGGGTTGTAGCCAACATGGGGGACAACTACGTTGTCGAGTACAAGGAGCTGTTGGAGGATGACGAGCGCACGCTCTACAAAGAAACCGTCATGGCTCACGAAGTCCGCCCTCCGCACCCGAACAAAACCCTACCTTCAAGCCCAAGCGGCTCATCTGCAAACAACCAAAAGTTTGGTTTCGGGGGTTTGGTGGATGCGTACGACAACGACGGGTGGTGGACAGGGGTCGTCACCGGGGAGAAGGGCGGTTTCTGCGCCGTGTTCTTCGAAACAACGTCGGAACACTTTGCTTATCCGGCTACTCACTTGAGGCCTCATCTGGATTGGAAGAAAGGGATGTGGTTTCCTGGCAAGAACAGGCGAAGGTTCTTGCATGAGAATCCAAATTTCTCAGAAGCCATGACTGAAGTTATCTTGTATGAAAAGATCGAGCAGTCGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

209

Amino Acids

23.59

Weight (kDa)

5.68

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 43 - 101 1.3e-15 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06340
fragaria_vesca FvH4_2g23230 FvH4_6g05270 FvH4_6g14470
malus_domestica MD04G1194400.v1.1 MD04G1194500.v1.1 MD04G1194700.v1.1 MD04G1194900.v1.1 MD04G1199300.v1.1 MD12G1207100.v1.1
prunus_persica Prupe.6G295700_v2.0.a1 Prupe.6G314400_v2.0.a1 Prupe.6G314500_v2.0.a1
pyrus_communis pycom04g17200 pycom12g19490
rosa_chinensis RchiOBHm_Chr1g0336721 RchiOBHm_Chr3g0454011 RchiOBHm_Chr3g0484961 RchiOBHm_Chr6g0290501 RchiOBHm_Chr6g0290521 RchiOBHm_Chr6g0290541 RchiOBHm_Chr7g0235761
rosa_laevigata RLG00000001142 RLG00000006528 RLG00000012205 RLG00000012207 RLG00000012209 RLG00000024490 RLG00000025490 RLG00000035862
rosa_multiflora Rmu_co8053086.1_g000001 Rmu_sc0000679.1_g000031 Rmu_sc0001089.1_g000020 Rmu_sc0001302.1_g000001 Rmu_sc0002477.1_g000009 Rmu_sc0002724.1_g000039 Rmu_sc0002890.1_g000020 Rmu_sc0002890.1_g000022 Rmu_sc0003556.1_g000036 Rmu_sc0009295.1_g000012 Rmu_sc0011632.1_g000017 Rmu_sc0019265.1_g000002
rosa_roxburghii Rroxscaffold_4G00326260 Rroxscaffold_6G00413590 Rroxscaffold_7G00176960 Rroxscaffold_7G00176980 Rroxscaffold_7G00177000
rosa_rugosa Rorug06G0212900
rosa_samantha Rh1AG103800 Rh1BG086300 Rh2AG280500 Rh2AG280600 Rh3AG054900 Rh3AG136100 Rh3BG056500 Rh3BG158000 Rh3CG055700 Rh3CG157800 Rh3DG057000 Rh3DG158600 Rh6AG323500 Rh6AG323700 Rh6BG330700 Rh6BG330900 Rh6BG331100 Rh6BG413200 Rh6CG337000 Rh6CG337100 Rh6CG337300 Rh6DG323900 Rh6DG324100 Rh6DG324300 Rh7DG436300
rosa_wichuraiana Rw3G004220 Rw3G012920 Rw6G028060 Rw6G028080 Rw7G037160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 289, 296, 330, 429
AclWI GGATC 3 cut(s) 13, 80, 175
AcsI RAATTY 1 cut(s) 568
AcuI CTGAAG 1 cut(s) 609
AfaI GTAC 3 cut(s) 173, 224, 383
AfiI CCNNNNNNNGG 2 cut(s) 111, 201
AgsI TTSAA 2 cut(s) 137, 321
AjnI CCWGG 1 cut(s) 529
AloI GAACNNNNNNTCC 2 cut(s) 143, 175
AluBI AGCT 2 cut(s) 182, 232
AluI AGCT 2 cut(s) 182, 232
AlwI GGATC 3 cut(s) 13, 80, 175
AoxI GGCC 2 cut(s) 5, 494
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 1 cut(s) 568
ArsI GACNNNNNNTTYG 2 cut(s) 255, 287
AspLEI GCGC 2 cut(s) 252, 440
AspS9I GGNCC 1 cut(s) 5
AsuC2I CCSGG 1 cut(s) 418
AsuHPI GGTGA 1 cut(s) 406
AsuII TTCGAA 2 cut(s) 117, 450
BbvI GCAGC 1 cut(s) 191
BceAI ACGGC 1 cut(s) 425
BciT130I CCWGG 1 cut(s) 531
BcnI CCSGG 1 cut(s) 418
BfaI CTAG 1 cut(s) 183
BglI GCCNNNNNGGC 1 cut(s) 330
BisI GCNGC 2 cut(s) 180, 331
BlsI GCNGC 2 cut(s) 181, 332
Bme1390I CCNGG 2 cut(s) 418, 531
BmgT120I GGNCC 1 cut(s) 5
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 418, 531
BmsI GCATC 1 cut(s) 367
BoxI GACNNNNGTC 1 cut(s) 407
Bpu14I TTCGAA 2 cut(s) 117, 450
BpuEI CTTGAG 1 cut(s) 511
BpuMI CCSGG 1 cut(s) 418
BsaJI CCNNGG 1 cut(s) 417
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 201
Bse1I ACTGG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 3 cut(s) 247, 382, 525
BseLI CCNNNNNNNGG 2 cut(s) 111, 201
BseMII CTCAG 2 cut(s) 84, 588
BseNI ACTGG 1 cut(s) 12
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 1 cut(s) 191
BshFI GGCC 2 cut(s) 7, 496
BsiSI CCGG 2 cut(s) 417, 479
BsiWI CGTACG 1 cut(s) 381
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 2 cut(s) 111, 201
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 2 cut(s) 7, 496
Bsp119I TTCGAA 2 cut(s) 117, 450
Bsp143I GATC 4 cut(s) 18, 85, 167, 609
BspACI CCGC 4 cut(s) 289, 296, 330, 429
BspANI GGCC 2 cut(s) 7, 496
BspCNI CTCAG 2 cut(s) 85, 587
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 3 cut(s) 13, 80, 175
BspT104I TTCGAA 2 cut(s) 117, 450
BsrI ACTGG 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 4 cut(s) 18, 85, 167, 609
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 1 cut(s) 271
BstBI TTCGAA 2 cut(s) 117, 450
BstC8I GCNNGC 1 cut(s) 254
BstDEI CTNAG 2 cut(s) 93, 574
BstF5I GGATG 3 cut(s) 247, 382, 525
BstHHI GCGC 2 cut(s) 252, 440
BstKTI GATC 4 cut(s) 21, 88, 170, 612
BstMBI GATC 4 cut(s) 18, 85, 167, 609
BstMWI GCNNNNNNNGC 3 cut(s) 330, 435, 479
BstNI CCWGG 1 cut(s) 531
BstPAI GACNNNNGTC 1 cut(s) 407
BstSCI CCNGG 2 cut(s) 416, 529
BstV1I GCAGC 1 cut(s) 191
BstXI CCANNNNNNTGG 1 cut(s) 356
BsuRI GGCC 2 cut(s) 7, 496
BtsCI GGATG 3 cut(s) 247, 382, 525
BtsIMutI CAGTG 1 cut(s) 19
Cac8I GCNNGC 1 cut(s) 254
CfoI GCGC 2 cut(s) 252, 440
Cfr13I GGNCC 1 cut(s) 5
Csp6I GTAC 3 cut(s) 172, 223, 382
CviAII CATG 4 cut(s) 199, 274, 557, 583
CviQI GTAC 3 cut(s) 172, 223, 382
DdeI CTNAG 2 cut(s) 93, 574
DpnI GATC 4 cut(s) 20, 87, 169, 611
DpnII GATC 4 cut(s) 18, 85, 167, 609
EciI GGCGGA 1 cut(s) 278
Eco147I AGGCCT 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 609
EcoO109I RGGNCCY 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 529
FaeI CATG 4 cut(s) 202, 277, 560, 586
FaiI YATR 5 cut(s) 200, 275, 558, 584, 603
FaqI GGGAC 1 cut(s) 218
FatI CATG 4 cut(s) 198, 273, 556, 582
Fnu4HI GCNGC 2 cut(s) 180, 331
FokI GGATG 3 cut(s) 254, 389, 532
Fsp4HI GCNGC 2 cut(s) 180, 331
FspBI CTAG 1 cut(s) 183
GlaI GCGC 2 cut(s) 251, 439
GluI GCNGC 2 cut(s) 180, 331
HaeIII GGCC 2 cut(s) 7, 496
HapII CCGG 2 cut(s) 417, 479
HhaI GCGC 2 cut(s) 252, 440
Hin1II CATG 4 cut(s) 202, 277, 560, 586
Hin6I GCGC 2 cut(s) 250, 438
HinP1I GCGC 2 cut(s) 250, 438
HinfI GANTC 1 cut(s) 562
HpaII CCGG 2 cut(s) 417, 479
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 1 cut(s) 402
Hpy188I TCNGA 5 cut(s) 85, 90, 167, 463, 577
Hpy188III TCNNGA 2 cut(s) 281, 503
Hpy8I GTNNAC 1 cut(s) 402
Hpy99I CGWCG 4 cut(s) 30, 43, 395, 463
HpyAV CCTTC 5 cut(s) 18, 63, 327, 418, 540
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4IV ACGT 3 cut(s) 25, 213, 458
HpyCH4V TGCA 2 cut(s) 341, 556
HpyF10VI GCNNNNNNNGC 3 cut(s) 330, 435, 479
HpyF3I CTNAG 2 cut(s) 93, 574
HpySE526I ACGT 3 cut(s) 25, 213, 458
Hsp92II CATG 4 cut(s) 202, 277, 560, 586
HspAI GCGC 2 cut(s) 250, 438
Kzo9I GATC 4 cut(s) 18, 85, 167, 609
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 8 cut(s) 25, 390, 430, 488, 492, 516, 526, 543
Lsp1109I GCAGC 1 cut(s) 191
LweI GCATC 1 cut(s) 367
MaeI CTAG 1 cut(s) 183
MaeII ACGT 3 cut(s) 25, 213, 458
MaeIII GTNAC 1 cut(s) 412
MalI GATC 4 cut(s) 20, 87, 169, 611
MboI GATC 4 cut(s) 18, 85, 167, 609
MboII GAAGA 2 cut(s) 439, 523
MluCI AATT 1 cut(s) 568
MmeI TCCRAC 4 cut(s) 46, 113, 216, 441
MnlI CCTC 9 cut(s) 73, 91, 124, 145, 148, 232, 303, 486, 507
MseI TTAA 1 cut(s) 143
MspI CCGG 2 cut(s) 417, 479
MspR9I CCNGG 2 cut(s) 418, 531
MvaI CCWGG 1 cut(s) 531
MwoI GCNNNNNNNGC 3 cut(s) 330, 435, 479
NciI CCSGG 1 cut(s) 418
NdeII GATC 4 cut(s) 18, 85, 167, 609
NlaIII CATG 4 cut(s) 202, 277, 560, 586
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 412
NspV TTCGAA 2 cut(s) 117, 450
PceI AGGCCT 1 cut(s) 496
PfeI GAWTC 1 cut(s) 562
Pfl23II CGTACG 1 cut(s) 381
PkrI GCNGC 2 cut(s) 181, 332
PshAI GACNNNNGTC 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspLI CGTACG 1 cut(s) 381
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 5
RsaI GTAC 3 cut(s) 173, 224, 383
RsaNI GTAC 3 cut(s) 172, 223, 382
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 2 cut(s) 180, 331
Sau3AI GATC 4 cut(s) 18, 85, 167, 609
Sau96I GGNCC 1 cut(s) 5
ScrFI CCNGG 2 cut(s) 418, 531
SetI ASST 9 cut(s) 28, 113, 135, 184, 216, 234, 319, 461, 551
SfaNI GCATC 1 cut(s) 367
SfuI TTCGAA 2 cut(s) 117, 450
SmlI CTYRAG 1 cut(s) 490
SmoI CTYRAG 1 cut(s) 490
Sse9I AATT 1 cut(s) 568
SseBI AGGCCT 1 cut(s) 496
SsiI CCGC 4 cut(s) 289, 296, 330, 429
SspMI CTAG 1 cut(s) 183
StuI AGGCCT 1 cut(s) 496
StyD4I CCNGG 2 cut(s) 416, 529
TaaI ACNGT 1 cut(s) 271
TaiI ACGT 3 cut(s) 28, 216, 461
TaqI TCGA 5 cut(s) 117, 219, 450, 612, 620
TasI AATT 1 cut(s) 568
TatI WGTACW 1 cut(s) 222
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 1 cut(s) 562
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 1 cut(s) 179
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 2 cut(s) 151, 618
TspRI CASTG 1 cut(s) 19
XapI RAATTY 1 cut(s) 568
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.