MD04G1194900.v1.1

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000631
Physical Location & Seq
Forward (+)
28391222 .. 28391860
639 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1194900.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGGGCCTTCCCGTGCGATCCAACCGCGTACAAACGCATCGAACTCTCGTCCTGCTTCCTCCTTTCGCTCACAACTCGACGATCCAAATGAGTTCCTCCCCGAAAGATGGTTTGAAACCAACAACGAAGTTGAAGTATCAAGCAGGGATGAGGGGTTCATCGGATCGCACTTTGCAGCTAGGGTTGTAGCCAACATGGGGGACAACTATGTTGTCGAGTACAAGGAGCTATTGGAGAATGACGAGCGCACGCTCTACAAAGAGACCGTCATGGCTCATGACGTCCGCCCTCCGCACCCGAATCAAACCCTGCCATTGAGCCCGAACGACTCATCTGCAAACAATGAAAAGTTTGGTTTCGGCGATTTGGTGGATGTGTACGACAATGACGGGTGGTGGAAGGGGGTTGTCACCGGGGAGAAGGATGGTTTCTACGCCGTGTTCTTCGAAACAACGTTGGATCACGTTGTTTATCCGGCTACTCACGTGAGGCCTCATCTGGATTGGAGGAGAGGGAAGTGGTTTCCTGGCAAGAACAGGGAAAGGTTCTTGCATGGGAACCCAAATTTCTCAGAAGAGATGACGCAAGTTATCTTGGATGAAAAGATCGAGCAGTCAAAGTTAAATACATCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

213

Amino Acids

24.35

Weight (kDa)

5.09

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 42 - 100 2.8e-14 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06340
fragaria_vesca FvH4_2g23230 FvH4_6g05270 FvH4_6g14470
malus_domestica MD04G1194400.v1.1 MD04G1194500.v1.1 MD04G1194700.v1.1 MD04G1194900.v1.1 MD04G1199300.v1.1 MD12G1207100.v1.1
prunus_persica Prupe.6G295700_v2.0.a1 Prupe.6G314400_v2.0.a1 Prupe.6G314500_v2.0.a1
pyrus_communis pycom04g17200 pycom12g19490
rosa_chinensis RchiOBHm_Chr1g0336721 RchiOBHm_Chr3g0454011 RchiOBHm_Chr3g0484961 RchiOBHm_Chr6g0290501 RchiOBHm_Chr6g0290521 RchiOBHm_Chr6g0290541 RchiOBHm_Chr7g0235761
rosa_laevigata RLG00000001142 RLG00000006528 RLG00000012205 RLG00000012207 RLG00000012209 RLG00000024490 RLG00000025490 RLG00000035862
rosa_multiflora Rmu_co8053086.1_g000001 Rmu_sc0000679.1_g000031 Rmu_sc0001089.1_g000020 Rmu_sc0001302.1_g000001 Rmu_sc0002477.1_g000009 Rmu_sc0002724.1_g000039 Rmu_sc0002890.1_g000020 Rmu_sc0002890.1_g000022 Rmu_sc0003556.1_g000036 Rmu_sc0009295.1_g000012 Rmu_sc0011632.1_g000017 Rmu_sc0019265.1_g000002
rosa_roxburghii Rroxscaffold_4G00326260 Rroxscaffold_6G00413590 Rroxscaffold_7G00176960 Rroxscaffold_7G00176980 Rroxscaffold_7G00177000
rosa_rugosa Rorug06G0212900
rosa_samantha Rh1AG103800 Rh1BG086300 Rh2AG280500 Rh2AG280600 Rh3AG054900 Rh3AG136100 Rh3BG056500 Rh3BG158000 Rh3CG055700 Rh3CG157800 Rh3DG057000 Rh3DG158600 Rh6AG323500 Rh6AG323700 Rh6BG330700 Rh6BG330900 Rh6BG331100 Rh6BG413200 Rh6CG337000 Rh6CG337100 Rh6CG337300 Rh6DG323900 Rh6DG324100 Rh6DG324300 Rh7DG436300
rosa_wichuraiana Rw3G004220 Rw3G012920 Rw6G028060 Rw6G028080 Rw7G037160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 285
AccII CGCG 1 cut(s) 28
AciI CCGC 3 cut(s) 26, 286, 293
AclI AACGTT 1 cut(s) 455
AclWI GGATC 4 cut(s) 13, 77, 172, 468
AcsI RAATTY 1 cut(s) 565
AcvI CACGTG 1 cut(s) 487
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 3 cut(s) 31, 221, 380
AfiI CCNNNNNNNGG 2 cut(s) 108, 198
AgsI TTSAA 2 cut(s) 116, 134
AjnI CCWGG 1 cut(s) 526
AloI GAACNNNNNNTCC 2 cut(s) 140, 172
AluBI AGCT 2 cut(s) 179, 229
AluI AGCT 2 cut(s) 179, 229
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 4 cut(s) 13, 77, 172, 468
AoxI GGCC 2 cut(s) 5, 491
ApeKI GCWGC 1 cut(s) 176
ApoI RAATTY 1 cut(s) 565
ArsI GACNNNNNNTTYG 2 cut(s) 252, 284
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 1 cut(s) 5
AsuC2I CCSGG 1 cut(s) 415
AsuHPI GGTGA 1 cut(s) 403
AsuII TTCGAA 1 cut(s) 447
BanII GRGCYC 1 cut(s) 323
BbrPI CACGTG 1 cut(s) 487
BbvI GCAGC 1 cut(s) 188
BccI CCATC 2 cut(s) 102, 419
BceAI ACGGC 1 cut(s) 422
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 1 cut(s) 528
BcnI CCSGG 1 cut(s) 415
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 1 cut(s) 180
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 2 cut(s) 415, 528
BmgT120I GGNCC 1 cut(s) 5
BmiI GGNNCC 2 cut(s) 6, 560
BmrFI CCNGG 2 cut(s) 415, 528
BmsI GCATC 1 cut(s) 47
Bpu14I TTCGAA 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 415
BsaAI YACGTR 1 cut(s) 487
BsaHI GRCGYC 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 414
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 198
BseBI CCWGG 1 cut(s) 528
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 4 cut(s) 154, 379, 430, 604
BseLI CCNNNNNNNGG 2 cut(s) 108, 198
BseMII CTCAG 1 cut(s) 585
BseRI GAGGAG 1 cut(s) 523
BseXI GCAGC 1 cut(s) 188
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 2 cut(s) 7, 493
BsiSI CCGG 2 cut(s) 414, 476
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 108, 198
BsmAI GTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 2 cut(s) 7, 493
Bso31I GGTCTC 1 cut(s) 257
Bsp119I TTCGAA 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 5 cut(s) 18, 82, 164, 460, 606
BspACI CCGC 3 cut(s) 26, 286, 293
BspANI GGCC 2 cut(s) 7, 493
BspCNI CTCAG 1 cut(s) 584
BspFNI CGCG 1 cut(s) 28
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 2 cut(s) 6, 560
BspPI GGATC 4 cut(s) 13, 77, 172, 468
BspT104I TTCGAA 1 cut(s) 447
BspTNI GGTCTC 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 5 cut(s) 18, 82, 164, 460, 606
BssNI GRCGYC 1 cut(s) 282
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 570
BstACI GRCGYC 1 cut(s) 282
BstBAI YACGTR 1 cut(s) 487
BstBI TTCGAA 1 cut(s) 447
BstC8I GCNNGC 1 cut(s) 251
BstDEI CTNAG 1 cut(s) 571
BstF5I GGATG 4 cut(s) 154, 379, 430, 604
BstFNI CGCG 1 cut(s) 28
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 5 cut(s) 21, 85, 167, 463, 609
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 5 cut(s) 18, 82, 164, 460, 606
BstNI CCWGG 1 cut(s) 528
BstSCI CCNGG 2 cut(s) 413, 526
BstUI CGCG 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 188
BsuRI GGCC 2 cut(s) 7, 493
BtsCI GGATG 4 cut(s) 154, 379, 430, 604
Cac8I GCNNGC 1 cut(s) 251
CciI TCATGA 1 cut(s) 277
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 5
CseI GACGC 1 cut(s) 592
Csp6I GTAC 3 cut(s) 30, 220, 379
CviAII CATG 4 cut(s) 196, 271, 278, 554
CviJI RGCY 8 cut(s) 7, 179, 191, 229, 275, 321, 479, 493
CviKI_1 RGCY 8 cut(s) 7, 179, 191, 229, 275, 321, 479, 493
CviQI GTAC 3 cut(s) 30, 220, 379
DdeI CTNAG 1 cut(s) 571
DpnI GATC 5 cut(s) 20, 84, 166, 462, 608
DpnII GATC 5 cut(s) 18, 82, 164, 460, 606
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
EciI GGCGGA 1 cut(s) 275
Eco147I AGGCCT 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 323
Eco31I GGTCTC 1 cut(s) 257
Eco72I CACGTG 1 cut(s) 487
EcoO109I RGGNCCY 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 323
FaeI CATG 4 cut(s) 199, 274, 281, 557
FaiI YATR 5 cut(s) 197, 210, 272, 279, 555
FaqI GGGAC 1 cut(s) 215
FatI CATG 4 cut(s) 195, 270, 277, 553
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 4 cut(s) 161, 386, 437, 611
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 1 cut(s) 180
GlaI GCGC 1 cut(s) 248
GluI GCNGC 1 cut(s) 177
HaeIII GGCC 2 cut(s) 7, 493
HapII CCGG 2 cut(s) 414, 476
HgaI GACGC 1 cut(s) 592
HhaI GCGC 1 cut(s) 249
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 4 cut(s) 199, 274, 281, 557
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HinfI GANTC 2 cut(s) 301, 329
HpaII CCGG 2 cut(s) 414, 476
HphI GGTGA 1 cut(s) 403
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 2 cut(s) 164, 574
Hpy188III TCNNGA 2 cut(s) 278, 500
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 3 cut(s) 18, 394, 415
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 4 cut(s) 282, 455, 465, 486
HpyCH4V TGCA 3 cut(s) 176, 338, 553
HpyF3I CTNAG 1 cut(s) 571
HpySE526I ACGT 4 cut(s) 282, 455, 465, 486
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 4 cut(s) 199, 274, 281, 557
HspAI GCGC 1 cut(s) 247
Kzo9I GATC 5 cut(s) 18, 82, 164, 460, 606
LmnI GCTCC 1 cut(s) 226
LpnPI CCDG 9 cut(s) 66, 130, 323, 427, 485, 489, 513, 523, 540
Lsp1109I GCAGC 1 cut(s) 188
LweI GCATC 1 cut(s) 47
MaeI CTAG 1 cut(s) 180
MaeII ACGT 4 cut(s) 282, 455, 465, 486
MaeIII GTNAC 1 cut(s) 409
MalI GATC 5 cut(s) 20, 84, 166, 462, 608
MboI GATC 5 cut(s) 18, 82, 164, 460, 606
MboII GAAGA 2 cut(s) 436, 587
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 1 cut(s) 565
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 2 cut(s) 46, 438
MnlI CCTC 8 cut(s) 70, 107, 145, 300, 483, 501, 504, 506
MseI TTAA 2 cut(s) 623, 637
MspI CCGG 2 cut(s) 414, 476
MspR9I CCNGG 2 cut(s) 415, 528
MvaI CCWGG 1 cut(s) 528
MvnI CGCG 1 cut(s) 28
NciI CCSGG 1 cut(s) 415
NdeII GATC 5 cut(s) 18, 82, 164, 460, 606
NlaIII CATG 4 cut(s) 199, 274, 281, 557
NlaIV GGNNCC 2 cut(s) 6, 560
NmuCI GTSAC 1 cut(s) 409
NspV TTCGAA 1 cut(s) 447
PagI TCATGA 1 cut(s) 277
PceI AGGCCT 1 cut(s) 493
PfeI GAWTC 1 cut(s) 301
PkrI GCNGC 1 cut(s) 178
PleI GAGTC 1 cut(s) 323
PmaCI CACGTG 1 cut(s) 487
PmlI CACGTG 1 cut(s) 487
PpsI GAGTC 1 cut(s) 323
Ppu21I YACGTR 1 cut(s) 487
Psp1406I AACGTT 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 526
PspCI CACGTG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 2 cut(s) 6, 560
PspPI GGNCC 1 cut(s) 5
RsaI GTAC 3 cut(s) 31, 221, 380
RsaNI GTAC 3 cut(s) 30, 220, 379
SaqAI TTAA 2 cut(s) 623, 637
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 5 cut(s) 18, 82, 164, 460, 606
Sau96I GGNCC 1 cut(s) 5
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 2 cut(s) 415, 528
SduI GDGCHC 1 cut(s) 323
SetI ASST 7 cut(s) 181, 231, 285, 458, 468, 489, 548
SfaNI GCATC 1 cut(s) 47
SfuI TTCGAA 1 cut(s) 447
Sse9I AATT 1 cut(s) 565
SseBI AGGCCT 1 cut(s) 493
SsiI CCGC 3 cut(s) 26, 286, 293
SspMI CTAG 1 cut(s) 180
StuI AGGCCT 1 cut(s) 493
StyD4I CCNGG 2 cut(s) 413, 526
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 4 cut(s) 285, 458, 468, 489
TaqI TCGA 5 cut(s) 41, 78, 216, 447, 609
TasI AATT 1 cut(s) 565
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 1 cut(s) 301
Tru1I TTAA 2 cut(s) 623, 637
Tru9I TTAA 2 cut(s) 623, 637
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 3 cut(s) 148, 360, 615
XapI RAATTY 1 cut(s) 565
XspI CTAG 1 cut(s) 180
ZraI GACGTC 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.