pycom04g17200

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000631
Physical Location & Seq
Reverse (-)
19686155 .. 19688118
1964 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g17200.1

Sequence Viewer

Length: 951 bp
ATGGAGGCATTCCGTATGGAAAGGTTTTTCGAAAGAGACAACGAGATCGAAGTGACCGGCAAGGAAAAGGGGTTTCTCGGATCATACTACGCAGCCAGGGTCATCGCCAACATGGGAGACAATTACGTGGTGGAGTACAAGGAGCTGCTGGAGGACGACCAGCGCACGCACTGTAAAGAAACCGTGATGGCTCATGACGTCCGCCCTCCGCACCCGAACCAAACCCTACCTTCAAGCCCAAGGGATTACTCATCTGCAAAGAAAAAGTTTGGTTTCGGCGAACAAGTGGATGCGTACGACAACGACGGCTGGTGGGTGGGGATCGTCACCGGGGAGAAAGGCGGTTTCTACACCGTGTTTTTCGAAACAACATGGGATCACATTGCGTATCCGGTTACTCACTTGAGGCCTCATCTGGAGTGGAGGAACGGAAGGTGGTTTCCTGGAAAAAACCGAGGTCGATTCGTGGCGGAGAACTCGTATTTTTCACATGAATTGGTGGAGTGTATCTTGGACGAAAAGATTGAGCAGGAAAAGGTGAAGAAATCGGAAGTTCAACAAGATCATGAGCATCATCGCATGCAGTCCGGGTTTTCAAAAGAACGACGAAATGAAGTGCGCAGCAAGGAGCAGGGCTTTCTCGCATCCTACTACGAAGCAACCGTGGTGTCAAACATGGGGGACAACTACGTGGTCGAATACAAGGAACTCTTAGAAGACGATGAGTTCACACCTCTCAACGAAACCGTTATGGTCAGCGAAGTCCGACCTCGACCCCCTTCCGCTCCTCAAACGGTGGCGAAGCAGAAGTTCACCTTCGGCGAAGTTGTCGACGCGTATGACAACGACGGCTGTTGGGTGGGAATCGTCACCAGGAAGAGAGGGAGTTACTGCACCTTCTTTTCCGAGAGCACTTGGGATCACATGTTGCTTATCCAGTCACTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

317

Amino Acids

36.84

Weight (kDa)

5.32

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 14 - 72 8e-14 Agenet domain
Agenet PF05641 205 - 260 4.9e-16 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06340
fragaria_vesca FvH4_2g23230 FvH4_6g05270 FvH4_6g14470
malus_domestica MD04G1194400.v1.1 MD04G1194500.v1.1 MD04G1194700.v1.1 MD04G1194900.v1.1 MD04G1199300.v1.1 MD12G1207100.v1.1
prunus_persica Prupe.6G295700_v2.0.a1 Prupe.6G314400_v2.0.a1 Prupe.6G314500_v2.0.a1
pyrus_communis pycom04g17200 pycom12g19490
rosa_chinensis RchiOBHm_Chr1g0336721 RchiOBHm_Chr3g0454011 RchiOBHm_Chr3g0484961 RchiOBHm_Chr6g0290501 RchiOBHm_Chr6g0290521 RchiOBHm_Chr6g0290541 RchiOBHm_Chr7g0235761
rosa_laevigata RLG00000001142 RLG00000006528 RLG00000012205 RLG00000012207 RLG00000012209 RLG00000024490 RLG00000025490 RLG00000035862
rosa_multiflora Rmu_co8053086.1_g000001 Rmu_sc0000679.1_g000031 Rmu_sc0001089.1_g000020 Rmu_sc0001302.1_g000001 Rmu_sc0002477.1_g000009 Rmu_sc0002724.1_g000039 Rmu_sc0002890.1_g000020 Rmu_sc0002890.1_g000022 Rmu_sc0003556.1_g000036 Rmu_sc0009295.1_g000012 Rmu_sc0011632.1_g000017 Rmu_sc0019265.1_g000002
rosa_roxburghii Rroxscaffold_4G00326260 Rroxscaffold_6G00413590 Rroxscaffold_7G00176960 Rroxscaffold_7G00176980 Rroxscaffold_7G00177000
rosa_rugosa Rorug06G0212900
rosa_samantha Rh1AG103800 Rh1BG086300 Rh2AG280500 Rh2AG280600 Rh3AG054900 Rh3AG136100 Rh3BG056500 Rh3BG158000 Rh3CG055700 Rh3CG157800 Rh3DG057000 Rh3DG158600 Rh6AG323500 Rh6AG323700 Rh6BG330700 Rh6BG330900 Rh6BG331100 Rh6BG413200 Rh6CG337000 Rh6CG337100 Rh6CG337300 Rh6DG323900 Rh6DG324100 Rh6DG324300 Rh7DG436300
rosa_wichuraiana Rw3G004220 Rw3G012920 Rw6G028060 Rw6G028080 Rw7G037160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 201
Acc16I TGCGCA 1 cut(s) 620
AccBSI CCGCTC 1 cut(s) 785
AccI GTMKAC 1 cut(s) 831
AccII CGCG 1 cut(s) 836
AciI CCGC 5 cut(s) 202, 209, 342, 470, 783
AclWI GGATC 4 cut(s) 88, 329, 384, 927
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 2 cut(s) 137, 296
AflIII ACRYGT 2 cut(s) 834, 924
AgsI TTSAA 3 cut(s) 234, 557, 597
AjnI CCWGG 3 cut(s) 95, 442, 872
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
Alw21I GWGCWC 1 cut(s) 914
Alw26I GTCTC 2 cut(s) 30, 111
AlwI GGATC 4 cut(s) 88, 329, 384, 927
AoxI GGCC 1 cut(s) 407
ApeKI GCWGC 3 cut(s) 92, 145, 621
AspLEI GCGC 2 cut(s) 165, 621
AsuC2I CCSGG 2 cut(s) 331, 589
AsuHPI GGTGA 4 cut(s) 319, 550, 805, 862
AsuII TTCGAA 2 cut(s) 30, 363
BbsI GAAGAC 1 cut(s) 723
Bbv12I GWGCWC 1 cut(s) 914
BbvI GCAGC 3 cut(s) 104, 132, 633
BccI CCATC 1 cut(s) 181
BceAI ACGGC 2 cut(s) 322, 865
BciT130I CCWGG 3 cut(s) 97, 444, 874
BciVI GTATCC 1 cut(s) 399
BcnI CCSGG 2 cut(s) 331, 589
BcoDI GTCTC 2 cut(s) 30, 111
BfuI GTATCC 1 cut(s) 399
BisI GCNGC 3 cut(s) 93, 146, 622
BlsI GCNGC 3 cut(s) 94, 147, 623
Bme1390I CCNGG 5 cut(s) 97, 331, 444, 589, 874
BmrFI CCNGG 5 cut(s) 97, 331, 444, 589, 874
BmsI GCATC 3 cut(s) 280, 580, 653
BpiI GAAGAC 1 cut(s) 723
BpmI CTGGAG 2 cut(s) 170, 437
Bpu14I TTCGAA 2 cut(s) 30, 363
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 2 cut(s) 331, 589
BsaAI YACGTR 2 cut(s) 127, 691
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 5 cut(s) 96, 239, 330, 454, 663
BsaWI WCCGGW 1 cut(s) 391
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 937
Bse3DI GCAATG 1 cut(s) 381
BseBI CCWGG 3 cut(s) 97, 444, 874
BseDI CCNNGG 5 cut(s) 96, 239, 330, 454, 663
BseGI GGATG 2 cut(s) 295, 644
BseMI GCAATG 1 cut(s) 381
BseNI ACTGG 1 cut(s) 937
BseRI GAGGAG 1 cut(s) 777
BseXI GCAGC 3 cut(s) 104, 132, 633
BsgI GTGCAG 1 cut(s) 877
Bsh1236I CGCG 1 cut(s) 836
BshFI GGCC 1 cut(s) 409
BsiHKAI GWGCWC 1 cut(s) 914
BsiSI CCGG 4 cut(s) 57, 330, 392, 588
BsiWI CGTACG 1 cut(s) 294
BslFI GGGAC 1 cut(s) 695
BsmAI GTCTC 2 cut(s) 30, 111
BsmFI GGGAC 1 cut(s) 695
BsmI GAATGC 1 cut(s) 8
BsnI GGCC 1 cut(s) 409
Bsp119I TTCGAA 2 cut(s) 30, 363
Bsp1286I GDGCHC 1 cut(s) 914
Bsp143I GATC 6 cut(s) 45, 80, 321, 376, 562, 919
BspACI CCGC 5 cut(s) 202, 209, 342, 470, 783
BspANI GGCC 1 cut(s) 409
BspFNI CGCG 1 cut(s) 836
BspHI TCATGA 2 cut(s) 193, 565
BspPI GGATC 4 cut(s) 88, 329, 384, 927
BspT104I TTCGAA 2 cut(s) 30, 363
BsrBI CCGCTC 1 cut(s) 785
BsrDI GCAATG 1 cut(s) 381
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 937
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 5 cut(s) 96, 239, 330, 454, 663
BssMI GATC 6 cut(s) 45, 80, 321, 376, 562, 919
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 239
Bst2UI CCWGG 3 cut(s) 97, 444, 874
Bst4CI ACNGT 6 cut(s) 173, 184, 355, 664, 748, 796
Bst6I CTCTTC 1 cut(s) 872
BstACI GRCGYC 1 cut(s) 198
BstBAI YACGTR 2 cut(s) 127, 691
BstBI TTCGAA 2 cut(s) 30, 363
BstC8I GCNNGC 2 cut(s) 167, 581
BstDEI CTNAG 1 cut(s) 712
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 2 cut(s) 295, 644
BstFNI CGCG 1 cut(s) 836
BstHHI GCGC 2 cut(s) 165, 621
BstKTI GATC 6 cut(s) 48, 83, 324, 379, 565, 922
BstMAI GTCTC 2 cut(s) 30, 111
BstMBI GATC 6 cut(s) 45, 80, 321, 376, 562, 919
BstNI CCWGG 3 cut(s) 97, 444, 874
BstNSI RCATGY 2 cut(s) 583, 928
BstSCI CCNGG 5 cut(s) 95, 329, 442, 587, 872
BstUI CGCG 1 cut(s) 836
BstV1I GCAGC 3 cut(s) 104, 132, 633
BstV2I GAAGAC 1 cut(s) 723
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 1 cut(s) 409
BtgI CCRYGG 1 cut(s) 663
BtgZI GCGATG 2 cut(s) 88, 560
BtsCI GGATG 2 cut(s) 295, 644
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 167, 581
CciI TCATGA 2 cut(s) 193, 565
CfoI GCGC 2 cut(s) 165, 621
Cfr10I RCCGGY 1 cut(s) 56
CseI GACGC 1 cut(s) 842
Csp6I GTAC 2 cut(s) 136, 295
CviAII CATG 8 cut(s) 112, 194, 372, 491, 566, 580, 676, 925
CviJI RGCY 8 cut(s) 95, 145, 191, 237, 309, 409, 636, 852
CviKI_1 RGCY 8 cut(s) 95, 145, 191, 237, 309, 409, 636, 852
CviQI GTAC 2 cut(s) 136, 295
DdeI CTNAG 1 cut(s) 712
DpnI GATC 6 cut(s) 47, 82, 323, 378, 564, 921
DpnII GATC 6 cut(s) 45, 80, 321, 376, 562, 919
Eam1104I CTCTTC 1 cut(s) 872
EarI CTCTTC 1 cut(s) 872
EciI GGCGGA 2 cut(s) 191, 485
Eco130I CCWWGG 1 cut(s) 239
Eco147I AGGCCT 1 cut(s) 409
EcoRII CCWGG 3 cut(s) 95, 442, 872
EcoT14I CCWWGG 1 cut(s) 239
ErhI CCWWGG 1 cut(s) 239
FaeI CATG 8 cut(s) 115, 197, 375, 494, 569, 583, 679, 928
FalI AAGNNNNNCTT 4 cut(s) 695, 727, 800, 832
FaqI GGGAC 1 cut(s) 695
FatI CATG 8 cut(s) 111, 193, 371, 490, 565, 579, 675, 924
FblI GTMKAC 1 cut(s) 831
Fnu4HI GCNGC 3 cut(s) 93, 146, 622
FokI GGATG 2 cut(s) 302, 631
Fsp4HI GCNGC 3 cut(s) 93, 146, 622
FspI TGCGCA 1 cut(s) 620
GlaI GCGC 2 cut(s) 164, 620
GluI GCNGC 3 cut(s) 93, 146, 622
GsuI CTGGAG 2 cut(s) 170, 437
HaeIII GGCC 1 cut(s) 409
HapII CCGG 4 cut(s) 57, 330, 392, 588
HgaI GACGC 1 cut(s) 842
HhaI GCGC 2 cut(s) 165, 621
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 8 cut(s) 115, 197, 375, 494, 569, 583, 679, 928
Hin6I GCGC 2 cut(s) 163, 619
HinP1I GCGC 2 cut(s) 163, 619
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HinfI GANTC 2 cut(s) 462, 864
HpaII CCGG 4 cut(s) 57, 330, 392, 588
HphI GGTGA 4 cut(s) 319, 550, 805, 862
Hpy166II GTNNAC 3 cut(s) 729, 813, 832
Hpy188I TCNGA 4 cut(s) 80, 550, 767, 907
Hpy188III TCNNGA 3 cut(s) 194, 416, 566
Hpy8I GTNNAC 3 cut(s) 729, 813, 832
Hpy99I CGWCG 4 cut(s) 308, 609, 836, 851
HpyAV CCTTC 5 cut(s) 240, 426, 789, 826, 907
HpyCH4III ACNGT 6 cut(s) 173, 184, 355, 664, 748, 796
HpyCH4IV ACGT 3 cut(s) 126, 198, 690
HpyCH4V TGCA 3 cut(s) 257, 583, 894
HpyF3I CTNAG 1 cut(s) 712
HpySE526I ACGT 3 cut(s) 126, 198, 690
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 8 cut(s) 115, 197, 375, 494, 569, 583, 679, 928
HspAI GCGC 2 cut(s) 163, 619
Kzo9I GATC 6 cut(s) 45, 80, 321, 376, 562, 919
LmnI GCTCC 3 cut(s) 142, 628, 790
Lsp1109I GCAGC 3 cut(s) 104, 132, 633
LweI GCATC 3 cut(s) 280, 580, 653
MaeII ACGT 3 cut(s) 126, 198, 690
MaeIII GTNAC 6 cut(s) 52, 325, 394, 868, 887, 939
MalI GATC 6 cut(s) 47, 82, 323, 378, 564, 921
MbiI CCGCTC 1 cut(s) 785
MboI GATC 6 cut(s) 45, 80, 321, 376, 562, 919
MboII GAAGA 3 cut(s) 553, 728, 889
MhlI GDGCHC 1 cut(s) 914
MluCI AATT 2 cut(s) 121, 494
MluI ACGCGT 1 cut(s) 834
MmeI TCCRAC 1 cut(s) 790
MspI CCGG 4 cut(s) 57, 330, 392, 588
MspR9I CCNGG 5 cut(s) 97, 331, 444, 589, 874
Mva1269I GAATGC 1 cut(s) 8
MvaI CCWGG 3 cut(s) 97, 444, 874
MvnI CGCG 1 cut(s) 836
NciI CCSGG 2 cut(s) 331, 589
NdeII GATC 6 cut(s) 45, 80, 321, 376, 562, 919
NlaIII CATG 8 cut(s) 115, 197, 375, 494, 569, 583, 679, 928
NmuCI GTSAC 4 cut(s) 52, 325, 868, 939
NsbI TGCGCA 1 cut(s) 620
NspI RCATGY 2 cut(s) 583, 928
NspV TTCGAA 2 cut(s) 30, 363
PaeI GCATGC 1 cut(s) 583
PagI TCATGA 2 cut(s) 193, 565
PceI AGGCCT 1 cut(s) 409
PciI ACATGT 1 cut(s) 924
PcsI WCGNNNNNNNCGW 1 cut(s) 660
PctI GAATGC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 462, 864
Pfl23II CGTACG 1 cut(s) 294
PfoI TCCNGGA 1 cut(s) 442
PkrI GCNGC 3 cut(s) 94, 147, 623
Ppu21I YACGTR 2 cut(s) 127, 691
PscI ACATGT 1 cut(s) 924
Psp6I CCWGG 3 cut(s) 95, 442, 872
PspGI CCWGG 3 cut(s) 95, 442, 872
PspLI CGTACG 1 cut(s) 294
RsaI GTAC 2 cut(s) 137, 296
RsaNI GTAC 2 cut(s) 136, 295
SalI GTCGAC 1 cut(s) 830
SatI GCNGC 3 cut(s) 93, 146, 622
Sau3AI GATC 6 cut(s) 45, 80, 321, 376, 562, 919
ScrFI CCNGG 5 cut(s) 97, 331, 444, 589, 874
SduI GDGCHC 1 cut(s) 914
SfaNI GCATC 3 cut(s) 280, 580, 653
SfuI TTCGAA 2 cut(s) 30, 363
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
SphI GCATGC 1 cut(s) 583
Sse9I AATT 2 cut(s) 121, 494
SseBI AGGCCT 1 cut(s) 409
SsiI CCGC 5 cut(s) 202, 209, 342, 470, 783
StuI AGGCCT 1 cut(s) 409
StyD4I CCNGG 5 cut(s) 95, 329, 442, 587, 872
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 6 cut(s) 173, 184, 355, 664, 748, 796
TaiI ACGT 3 cut(s) 129, 201, 693
TaqI TCGA 7 cut(s) 30, 48, 363, 460, 696, 772, 831
TasI AATT 2 cut(s) 121, 494
TatI WGTACW 1 cut(s) 135
TfiI GAWTC 2 cut(s) 462, 864
TscAI CASTG 1 cut(s) 176
TseFI GTSAC 4 cut(s) 52, 325, 868, 939
TseI GCWGC 3 cut(s) 92, 145, 621
Tsp45I GTSAC 4 cut(s) 52, 325, 868, 939
TspDTI ATGAA 2 cut(s) 507, 627
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 1 cut(s) 176
XceI RCATGY 2 cut(s) 583, 928
XmiI GTMKAC 1 cut(s) 831
ZraI GACGTC 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.