MD04G1194700.v1.1

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000631
Physical Location & Seq
Reverse (-)
28383094 .. 28383726
633 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1194700.v1.1.491

Sequence Viewer

Length: 633 bp
ATGGAGGCATTCCGTATGGAAAGGTTTTTCGAAAGAGACAACGAGATCGAAGTGACCGGCAAGGAAAAGGGGTTTCTCGGATCTTACTACGCAGCCAGGGTCATCGCCAACATGGGAGACAATTACGTGGTGGAGTACAAGGAGCTGCTGGAGGACGACGAGCGCACGCACTGTAAAGAAACCGTGATGGCTCATGACGTCCGCCCTCCGCACCCGAACCAAACCCTACCTTCAAGCCCAAGGGACTACTCATCTGCAAAGAAAAAGTTTGGTTTCGGCGAACAAGTGGATGCGTACGACAACGACGGCTGGTGGGTGGGGATCGTCACCGGGGAGAAAGGCGGTTTCTGCACCGTGTTTTTCGAAACAACATGGGATCACATTGCGTATCCGGTTACTCACTTGAGGCCTCATCTGGAGTGGAGGAACGGAAGGTGGTTTCCTGGAAAAAACCGAGGCCGATTCGTGGTGGAGAACTCGTATTTTTCAAGTGAATTGGTGGAGTGTATCTTGGACGAAAAGATTGAGCAGGAAAAGTTGAAGAAATTGGAAGTTCAACGAGATCATGAGCATCATCGCATGCAGTCCGGCTGGGAAGAAAAGATGGTGCAGTCAAGAACTGTTTGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

211

Amino Acids

24.8

Weight (kDa)

5.64

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 14 - 72 1.2e-14 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06340
fragaria_vesca FvH4_2g23230 FvH4_6g05270 FvH4_6g14470
malus_domestica MD04G1194400.v1.1 MD04G1194500.v1.1 MD04G1194700.v1.1 MD04G1194900.v1.1 MD04G1199300.v1.1 MD12G1207100.v1.1
prunus_persica Prupe.6G295700_v2.0.a1 Prupe.6G314400_v2.0.a1 Prupe.6G314500_v2.0.a1
pyrus_communis pycom04g17200 pycom12g19490
rosa_chinensis RchiOBHm_Chr1g0336721 RchiOBHm_Chr3g0454011 RchiOBHm_Chr3g0484961 RchiOBHm_Chr6g0290501 RchiOBHm_Chr6g0290521 RchiOBHm_Chr6g0290541 RchiOBHm_Chr7g0235761
rosa_laevigata RLG00000001142 RLG00000006528 RLG00000012205 RLG00000012207 RLG00000012209 RLG00000024490 RLG00000025490 RLG00000035862
rosa_multiflora Rmu_co8053086.1_g000001 Rmu_sc0000679.1_g000031 Rmu_sc0001089.1_g000020 Rmu_sc0001302.1_g000001 Rmu_sc0002477.1_g000009 Rmu_sc0002724.1_g000039 Rmu_sc0002890.1_g000020 Rmu_sc0002890.1_g000022 Rmu_sc0003556.1_g000036 Rmu_sc0009295.1_g000012 Rmu_sc0011632.1_g000017 Rmu_sc0019265.1_g000002
rosa_roxburghii Rroxscaffold_4G00326260 Rroxscaffold_6G00413590 Rroxscaffold_7G00176960 Rroxscaffold_7G00176980 Rroxscaffold_7G00177000
rosa_rugosa Rorug06G0212900
rosa_samantha Rh1AG103800 Rh1BG086300 Rh2AG280500 Rh2AG280600 Rh3AG054900 Rh3AG136100 Rh3BG056500 Rh3BG158000 Rh3CG055700 Rh3CG157800 Rh3DG057000 Rh3DG158600 Rh6AG323500 Rh6AG323700 Rh6BG330700 Rh6BG330900 Rh6BG331100 Rh6BG413200 Rh6CG337000 Rh6CG337100 Rh6CG337300 Rh6DG323900 Rh6DG324100 Rh6DG324300 Rh7DG436300
rosa_wichuraiana Rw3G004220 Rw3G012920 Rw6G028060 Rw6G028080 Rw7G037160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 201
AciI CCGC 3 cut(s) 202, 209, 342
AclWI GGATC 3 cut(s) 88, 329, 384
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 2 cut(s) 137, 296
AfiI CCNNNNNNNGG 1 cut(s) 466
AgsI TTSAA 4 cut(s) 234, 489, 541, 557
AjnI CCWGG 2 cut(s) 95, 442
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
Alw26I GTCTC 2 cut(s) 30, 111
AlwI GGATC 3 cut(s) 88, 329, 384
AoxI GGCC 2 cut(s) 407, 457
ApeKI GCWGC 2 cut(s) 92, 145
AspLEI GCGC 1 cut(s) 165
AsuC2I CCSGG 1 cut(s) 331
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 2 cut(s) 30, 363
BbvI GCAGC 2 cut(s) 104, 132
BccI CCATC 2 cut(s) 181, 598
BceAI ACGGC 1 cut(s) 322
BciT130I CCWGG 2 cut(s) 97, 444
BciVI GTATCC 1 cut(s) 399
BcnI CCSGG 1 cut(s) 331
BcoDI GTCTC 2 cut(s) 30, 111
BfuI GTATCC 1 cut(s) 399
BisI GCNGC 2 cut(s) 93, 146
BlsI GCNGC 2 cut(s) 94, 147
Bme1390I CCNGG 3 cut(s) 97, 331, 444
BmrFI CCNGG 3 cut(s) 97, 331, 444
BmsI GCATC 2 cut(s) 280, 580
BpmI CTGGAG 2 cut(s) 170, 437
Bpu14I TTCGAA 2 cut(s) 30, 363
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 1 cut(s) 331
BsaAI YACGTR 1 cut(s) 127
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 4 cut(s) 96, 239, 330, 454
BsaWI WCCGGW 1 cut(s) 391
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse118I RCCGGY 1 cut(s) 56
Bse3DI GCAATG 1 cut(s) 381
BseBI CCWGG 2 cut(s) 97, 444
BseDI CCNNGG 4 cut(s) 96, 239, 330, 454
BseGI GGATG 1 cut(s) 295
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMI GCAATG 1 cut(s) 381
BseXI GCAGC 2 cut(s) 104, 132
BseYI CCCAGC 1 cut(s) 591
BsgI GTGCAG 2 cut(s) 334, 629
BshFI GGCC 2 cut(s) 409, 459
BsiSI CCGG 4 cut(s) 57, 330, 392, 588
BsiWI CGTACG 1 cut(s) 294
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 2 cut(s) 30, 111
BsmFI GGGAC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 8
BsnI GGCC 2 cut(s) 409, 459
Bsp119I TTCGAA 2 cut(s) 30, 363
Bsp143I GATC 5 cut(s) 45, 80, 321, 376, 562
BspACI CCGC 3 cut(s) 202, 209, 342
BspANI GGCC 2 cut(s) 409, 459
BspHI TCATGA 2 cut(s) 193, 565
BspPI GGATC 3 cut(s) 88, 329, 384
BspT104I TTCGAA 2 cut(s) 30, 363
BsrDI GCAATG 1 cut(s) 381
BsrFI RCCGGY 1 cut(s) 56
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 4 cut(s) 96, 239, 330, 454
BssMI GATC 5 cut(s) 45, 80, 321, 376, 562
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 239
Bst2UI CCWGG 2 cut(s) 97, 444
Bst4CI ACNGT 4 cut(s) 173, 184, 355, 622
BstACI GRCGYC 1 cut(s) 198
BstBAI YACGTR 1 cut(s) 127
BstBI TTCGAA 2 cut(s) 30, 363
BstC8I GCNNGC 2 cut(s) 167, 581
BstF5I GGATG 1 cut(s) 295
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 5 cut(s) 48, 83, 324, 379, 565
BstMAI GTCTC 2 cut(s) 30, 111
BstMBI GATC 5 cut(s) 45, 80, 321, 376, 562
BstMWI GCNNNNNNNGC 1 cut(s) 348
BstNI CCWGG 2 cut(s) 97, 444
BstNSI RCATGY 1 cut(s) 583
BstSCI CCNGG 3 cut(s) 95, 329, 442
BstV1I GCAGC 2 cut(s) 104, 132
BstX2I RGATCY 1 cut(s) 80
BstYI RGATCY 1 cut(s) 80
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 2 cut(s) 409, 459
BtgZI GCGATG 2 cut(s) 88, 560
BtsCI GGATG 1 cut(s) 295
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 167, 581
CciI TCATGA 2 cut(s) 193, 565
CfoI GCGC 1 cut(s) 165
Cfr10I RCCGGY 1 cut(s) 56
Csp6I GTAC 2 cut(s) 136, 295
CviAII CATG 5 cut(s) 112, 194, 372, 566, 580
CviJI RGCY 9 cut(s) 95, 145, 191, 237, 309, 409, 459, 591, 629
CviKI_1 RGCY 9 cut(s) 95, 145, 191, 237, 309, 409, 459, 591, 629
CviQI GTAC 2 cut(s) 136, 295
DpnI GATC 5 cut(s) 47, 82, 323, 378, 564
DpnII GATC 5 cut(s) 45, 80, 321, 376, 562
EciI GGCGGA 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 239
Eco147I AGGCCT 1 cut(s) 409
EcoRII CCWGG 2 cut(s) 95, 442
EcoT14I CCWWGG 1 cut(s) 239
ErhI CCWWGG 1 cut(s) 239
FaeI CATG 5 cut(s) 115, 197, 375, 569, 583
FaiI YATR 6 cut(s) 17, 113, 195, 373, 567, 581
FaqI GGGAC 1 cut(s) 257
FatI CATG 5 cut(s) 111, 193, 371, 565, 579
Fnu4HI GCNGC 2 cut(s) 93, 146
FokI GGATG 1 cut(s) 302
Fsp4HI GCNGC 2 cut(s) 93, 146
GlaI GCGC 1 cut(s) 164
GluI GCNGC 2 cut(s) 93, 146
GsaI CCCAGC 1 cut(s) 595
GsuI CTGGAG 2 cut(s) 170, 437
HaeIII GGCC 2 cut(s) 409, 459
HapII CCGG 4 cut(s) 57, 330, 392, 588
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 5 cut(s) 115, 197, 375, 569, 583
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HinfI GANTC 1 cut(s) 462
HpaII CCGG 4 cut(s) 57, 330, 392, 588
HphI GGTGA 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 80
Hpy188III TCNNGA 4 cut(s) 194, 416, 566, 615
Hpy99I CGWCG 2 cut(s) 161, 308
HpyAV CCTTC 2 cut(s) 240, 426
HpyCH4III ACNGT 4 cut(s) 173, 184, 355, 622
HpyCH4IV ACGT 2 cut(s) 126, 198
HpyCH4V TGCA 4 cut(s) 257, 351, 583, 610
HpyF10VI GCNNNNNNNGC 1 cut(s) 348
HpySE526I ACGT 2 cut(s) 126, 198
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 5 cut(s) 115, 197, 375, 569, 583
HspAI GCGC 1 cut(s) 163
Kzo9I GATC 5 cut(s) 45, 80, 321, 376, 562
LmnI GCTCC 1 cut(s) 142
Lsp1109I GCAGC 2 cut(s) 104, 132
LweI GCATC 2 cut(s) 280, 580
MaeII ACGT 2 cut(s) 126, 198
MaeIII GTNAC 3 cut(s) 52, 325, 394
MalI GATC 5 cut(s) 47, 82, 323, 378, 564
MboI GATC 5 cut(s) 45, 80, 321, 376, 562
MboII GAAGA 2 cut(s) 553, 608
MflI RGATCY 1 cut(s) 80
MluCI AATT 3 cut(s) 121, 494, 545
MnlI CCTC 6 cut(s) 145, 216, 399, 417, 420, 449
MspI CCGG 4 cut(s) 57, 330, 392, 588
MspR9I CCNGG 3 cut(s) 97, 331, 444
Mva1269I GAATGC 1 cut(s) 8
MvaI CCWGG 2 cut(s) 97, 444
MwoI GCNNNNNNNGC 1 cut(s) 348
NciI CCSGG 1 cut(s) 331
NdeII GATC 5 cut(s) 45, 80, 321, 376, 562
NlaIII CATG 5 cut(s) 115, 197, 375, 569, 583
NmuCI GTSAC 2 cut(s) 52, 325
NspI RCATGY 1 cut(s) 583
NspV TTCGAA 2 cut(s) 30, 363
PaeI GCATGC 1 cut(s) 583
PagI TCATGA 2 cut(s) 193, 565
PceI AGGCCT 1 cut(s) 409
PctI GAATGC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 462
Pfl23II CGTACG 1 cut(s) 294
PfoI TCCNGGA 1 cut(s) 442
PkrI GCNGC 2 cut(s) 94, 147
Ppu21I YACGTR 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 95, 442
PspFI CCCAGC 1 cut(s) 591
PspGI CCWGG 2 cut(s) 95, 442
PspLI CGTACG 1 cut(s) 294
PsuI RGATCY 1 cut(s) 80
RsaI GTAC 2 cut(s) 137, 296
RsaNI GTAC 2 cut(s) 136, 295
SatI GCNGC 2 cut(s) 93, 146
Sau3AI GATC 5 cut(s) 45, 80, 321, 376, 562
ScrFI CCNGG 3 cut(s) 97, 331, 444
SetI ASST 6 cut(s) 26, 129, 147, 201, 232, 437
SfaNI GCATC 2 cut(s) 280, 580
SfuI TTCGAA 2 cut(s) 30, 363
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
SphI GCATGC 1 cut(s) 583
Sse9I AATT 3 cut(s) 121, 494, 545
SseBI AGGCCT 1 cut(s) 409
SsiI CCGC 3 cut(s) 202, 209, 342
StuI AGGCCT 1 cut(s) 409
StyD4I CCNGG 3 cut(s) 95, 329, 442
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 4 cut(s) 173, 184, 355, 622
TaiI ACGT 2 cut(s) 129, 201
TaqI TCGA 3 cut(s) 30, 48, 363
TasI AATT 3 cut(s) 121, 494, 545
TatI WGTACW 1 cut(s) 135
TfiI GAWTC 1 cut(s) 462
TscAI CASTG 1 cut(s) 176
TseFI GTSAC 2 cut(s) 52, 325
TseI GCWGC 2 cut(s) 92, 145
Tsp45I GTSAC 2 cut(s) 52, 325
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 1 cut(s) 176
XceI RCATGY 1 cut(s) 583
ZraI GACGTC 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.