MD04G1199300.v1.1

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000631
Physical Location & Seq
Forward (+)
28641232 .. 28641648
417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1199300.v1.1.491

Sequence Viewer

Length: 417 bp
ATGGCGGACGAGGTCCGGCCTCTTCCTCCTCAGCCTTCTCAAGAAAAAGCGGATTTAGGGTTTGATGTCCTTGACAGGGTCGATGTGTTGGACAACAAGGGTTGGTGGGTAGGTACCGTTACTGAGAAGAGAGGGGAGGACGAATACTGGGTGTACTTTGAAACGACTGCGGATCATATTGCGTACCCTAAGAGGATCCTGAGATTTCATAAGGACTGGATCGATGGAATGTGGGTGAATTACAACAAGAAGAAGAGAGCTTTTCGGGCCGACGGAAAGCCGGTGGAGTGCAAGAAGAGAACGCGCGGGTCGATGAAAGGTTTGAAGACTACAAGAAAGTGCGCATCGATGGAAAGTTGGTGGATTGCAAGAGCAAGTTATAAGAGTCAGCATTGTATCTCTTGTTTAAATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

139

Amino Acids

16.21

Weight (kDa)

9.3

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06340
fragaria_vesca FvH4_2g23230 FvH4_6g05270 FvH4_6g14470
malus_domestica MD04G1194400.v1.1 MD04G1194500.v1.1 MD04G1194700.v1.1 MD04G1194900.v1.1 MD04G1199300.v1.1 MD12G1207100.v1.1
prunus_persica Prupe.6G295700_v2.0.a1 Prupe.6G314400_v2.0.a1 Prupe.6G314500_v2.0.a1
pyrus_communis pycom04g17200 pycom12g19490
rosa_chinensis RchiOBHm_Chr1g0336721 RchiOBHm_Chr3g0454011 RchiOBHm_Chr3g0484961 RchiOBHm_Chr6g0290501 RchiOBHm_Chr6g0290521 RchiOBHm_Chr6g0290541 RchiOBHm_Chr7g0235761
rosa_laevigata RLG00000001142 RLG00000006528 RLG00000012205 RLG00000012207 RLG00000012209 RLG00000024490 RLG00000025490 RLG00000035862
rosa_multiflora Rmu_co8053086.1_g000001 Rmu_sc0000679.1_g000031 Rmu_sc0001089.1_g000020 Rmu_sc0001302.1_g000001 Rmu_sc0002477.1_g000009 Rmu_sc0002724.1_g000039 Rmu_sc0002890.1_g000020 Rmu_sc0002890.1_g000022 Rmu_sc0003556.1_g000036 Rmu_sc0009295.1_g000012 Rmu_sc0011632.1_g000017 Rmu_sc0019265.1_g000002
rosa_roxburghii Rroxscaffold_4G00326260 Rroxscaffold_6G00413590 Rroxscaffold_7G00176960 Rroxscaffold_7G00176980 Rroxscaffold_7G00177000
rosa_rugosa Rorug06G0212900
rosa_samantha Rh1AG103800 Rh1BG086300 Rh2AG280500 Rh2AG280600 Rh3AG054900 Rh3AG136100 Rh3BG056500 Rh3BG158000 Rh3CG055700 Rh3CG157800 Rh3DG057000 Rh3DG158600 Rh6AG323500 Rh6AG323700 Rh6BG330700 Rh6BG330900 Rh6BG331100 Rh6BG413200 Rh6CG337000 Rh6CG337100 Rh6CG337300 Rh6DG323900 Rh6DG324100 Rh6DG324300 Rh7DG436300
rosa_wichuraiana Rw3G004220 Rw3G012920 Rw6G028060 Rw6G028080 Rw7G037160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 381
Acc16I TGCGCA 1 cut(s) 343
Acc65I GGTACC 1 cut(s) 113
AccB1I GGYRCC 1 cut(s) 113
AccII CGCG 2 cut(s) 304, 306
AciI CCGC 4 cut(s) 5, 50, 170, 306
AclWI GGATC 4 cut(s) 180, 190, 203, 227
AfaI GTAC 3 cut(s) 115, 155, 185
AfiI CCNNNNNNNGG 1 cut(s) 76
AgsI TTSAA 2 cut(s) 161, 325
AluBI AGCT 1 cut(s) 260
AluI AGCT 1 cut(s) 260
AlwI GGATC 4 cut(s) 180, 190, 203, 227
AoxI GGCC 2 cut(s) 17, 267
Asp718I GGTACC 1 cut(s) 113
AspLEI GCGC 2 cut(s) 306, 344
AspS9I GGNCC 2 cut(s) 13, 267
AsuHPI GGTGA 1 cut(s) 247
AvaII GGWCC 1 cut(s) 13
BamHI GGATCC 1 cut(s) 195
BanI GGYRCC 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 332
BbvCI CCTCAGC 1 cut(s) 30
BccI CCATC 2 cut(s) 218, 343
Bme18I GGWCC 1 cut(s) 13
BmgT120I GGNCC 2 cut(s) 13, 267
BmiI GGNNCC 2 cut(s) 115, 197
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 353
BmuI ACTGGG 1 cut(s) 157
BpiI GAAGAC 1 cut(s) 332
Bpu10I CCTNAGC 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 24
Bsa29I ATCGAT 2 cut(s) 222, 347
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 2 cut(s) 152, 221
BseCI ATCGAT 2 cut(s) 222, 347
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMII CTCAG 3 cut(s) 44, 114, 191
BseNI ACTGG 2 cut(s) 152, 221
BseRI GAGGAG 1 cut(s) 18
Bsh1236I CGCG 2 cut(s) 304, 306
BshFI GGCC 2 cut(s) 19, 269
BshNI GGYRCC 1 cut(s) 113
BshVI ATCGAT 2 cut(s) 222, 347
BsiSI CCGG 2 cut(s) 16, 281
BslI CCNNNNNNNGG 1 cut(s) 76
BsnI GGCC 2 cut(s) 19, 269
Bsp143I GATC 3 cut(s) 172, 195, 219
BspACI CCGC 4 cut(s) 5, 50, 170, 306
BspANI GGCC 2 cut(s) 19, 269
BspCNI CTCAG 3 cut(s) 43, 115, 192
BspDI ATCGAT 2 cut(s) 222, 347
BspFNI CGCG 2 cut(s) 304, 306
BspLI GGNNCC 2 cut(s) 115, 197
BspPI GGATC 4 cut(s) 180, 190, 203, 227
BspT107I GGYRCC 1 cut(s) 113
BsrFI RCCGGY 1 cut(s) 280
BsrI ACTGG 2 cut(s) 152, 221
BssAI RCCGGY 1 cut(s) 280
BssMI GATC 3 cut(s) 172, 195, 219
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 4 cut(s) 27, 122, 248, 290
BstDEI CTNAG 4 cut(s) 30, 123, 189, 200
BstFNI CGCG 2 cut(s) 304, 306
BstHHI GCGC 2 cut(s) 306, 344
BstKTI GATC 3 cut(s) 175, 198, 222
BstMBI GATC 3 cut(s) 172, 195, 219
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstUI CGCG 2 cut(s) 304, 306
BstV2I GAAGAC 1 cut(s) 332
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
Bsu15I ATCGAT 2 cut(s) 222, 347
BsuRI GGCC 2 cut(s) 19, 269
BsuTUI ATCGAT 2 cut(s) 222, 347
CfoI GCGC 2 cut(s) 306, 344
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 2 cut(s) 13, 267
ClaI ATCGAT 2 cut(s) 222, 347
Csp6I GTAC 3 cut(s) 114, 154, 184
CviJI RGCY 5 cut(s) 19, 34, 260, 269, 280
CviKI_1 RGCY 5 cut(s) 19, 34, 260, 269, 280
CviQI GTAC 3 cut(s) 114, 154, 184
DdeI CTNAG 4 cut(s) 30, 123, 189, 200
DpnI GATC 3 cut(s) 174, 197, 221
DpnII GATC 3 cut(s) 172, 195, 219
DraI TTTAAA 1 cut(s) 408
Eam1104I CTCTTC 4 cut(s) 27, 122, 248, 290
EarI CTCTTC 4 cut(s) 27, 122, 248, 290
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 13
FaiI YATR 3 cut(s) 177, 210, 381
FauI CCCGC 1 cut(s) 299
FspAI RTGCGCAY 1 cut(s) 343
FspI TGCGCA 1 cut(s) 343
GlaI GCGC 2 cut(s) 305, 343
HaeIII GGCC 2 cut(s) 19, 269
HapII CCGG 2 cut(s) 16, 281
HhaI GCGC 2 cut(s) 306, 344
Hin6I GCGC 2 cut(s) 304, 342
HinP1I GCGC 2 cut(s) 304, 342
HinfI GANTC 1 cut(s) 385
HpaII CCGG 2 cut(s) 16, 281
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 154
Hpy188III TCNNGA 2 cut(s) 41, 199
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 1 cut(s) 45
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4V TGCA 2 cut(s) 291, 368
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 4 cut(s) 30, 123, 189, 200
HspAI GCGC 2 cut(s) 304, 342
KpnI GGTACC 1 cut(s) 117
Kzo9I GATC 3 cut(s) 172, 195, 219
LpnPI CCDG 6 cut(s) 29, 61, 133, 202, 212, 294
LweI GCATC 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 118
MalI GATC 3 cut(s) 174, 197, 221
MboI GATC 3 cut(s) 172, 195, 219
MboII GAAGA 6 cut(s) 14, 139, 262, 265, 307, 337
MflI RGATCY 1 cut(s) 195
MluCI AATT 1 cut(s) 238
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 69
MnlI CCTC 7 cut(s) 4, 30, 36, 39, 125, 130, 186
MseI TTAA 1 cut(s) 407
MspI CCGG 2 cut(s) 16, 281
MvnI CGCG 2 cut(s) 304, 306
MwoI GCNNNNNNNGC 1 cut(s) 266
NdeII GATC 3 cut(s) 172, 195, 219
NlaIV GGNNCC 2 cut(s) 115, 197
NsbI TGCGCA 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 308
PflFI GACNNNGTC 2 cut(s) 11, 77
PleI GAGTC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 393
PsiI TTATAA 1 cut(s) 381
PspN4I GGNNCC 2 cut(s) 115, 197
PspPI GGNCC 2 cut(s) 13, 267
PsuI RGATCY 1 cut(s) 195
PsyI GACNNNGTC 2 cut(s) 11, 77
RsaI GTAC 3 cut(s) 115, 155, 185
RsaNI GTAC 3 cut(s) 114, 154, 184
SaqAI TTAA 1 cut(s) 407
Sau3AI GATC 3 cut(s) 172, 195, 219
Sau96I GGNCC 2 cut(s) 13, 267
SchI GAGTC 1 cut(s) 394
SetI ASST 4 cut(s) 15, 115, 262, 322
SfaNI GCATC 1 cut(s) 353
SinI GGWCC 1 cut(s) 13
SmlI CTYRAG 1 cut(s) 39
SmoI CTYRAG 1 cut(s) 39
Sse9I AATT 1 cut(s) 238
SsiI CCGC 4 cut(s) 5, 50, 170, 306
TaaI ACNGT 1 cut(s) 118
TaqI TCGA 4 cut(s) 81, 222, 311, 347
TasI AATT 1 cut(s) 238
TatI WGTACW 1 cut(s) 153
Tru1I TTAA 1 cut(s) 407
Tru9I TTAA 1 cut(s) 407
TspDTI ATGAA 2 cut(s) 197, 329
TspGWI ACGGA 1 cut(s) 288
Tth111I GACNNNGTC 2 cut(s) 11, 77
VpaK11BI GGWCC 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.