MD05G1136000.v1.1

Epidermal growth factor receptor substrate 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001694
Physical Location & Seq
Forward (+)
26425788 .. 26427791
2004 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1136000.v1.1.491

Sequence Viewer

Length: 417 bp
ATGGTCTGGTCTTCTGCGATTCGTAATCCGACGGTGGCGATGGCGTCGGGGCAGAAACAATCGGCGAATGTGGATCTCTTCGATGCGTATTTCCGACAAGCCGATTTGGACCGCGATGACCAGATTAGTGGCAATGAAGCCGTCGCTTTCTTCCAGGGCTCCGATCTCCCCAAACAGGTCCTTGCGCAGCAACCCATGCCTGGTCCTGGTGCTCGGCACATGGCAACCCCAGCTGGAGGAAGGCCACCGAAGCCAGTTGCTCCTTCTCATACTGAGGAAAATCAGCAGGCCAAGCAGCAGAAACCTAGAGTACCAGAATTGGAGAAACATCTTGTAAATCAATTGAGTACAGAGGAGGTTAACTCACTGAACTCAAAGTTCAAAGAAGCAACCGAAGCTGATAAAAGAGGTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_4 EF-hand_5

Protein Analysis

139

Amino Acids

15.09

Weight (kDa)

6.29

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 186
AccII CGCG 1 cut(s) 114
AciI CCGC 1 cut(s) 112
AclWI GGATC 1 cut(s) 81
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 3 cut(s) 312, 349, 412
AfiI CCNNNNNNNGG 4 cut(s) 175, 200, 206, 236
AgsI TTSAA 1 cut(s) 382
AjnI CCWGG 3 cut(s) 153, 199, 205
AluBI AGCT 2 cut(s) 233, 398
AluI AGCT 2 cut(s) 233, 398
Alw21I GWGCWC 1 cut(s) 214
AlwI GGATC 1 cut(s) 81
AoxI GGCC 2 cut(s) 242, 288
ApeKI GCWGC 2 cut(s) 187, 295
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 3 cut(s) 109, 178, 203
AvaII GGWCC 3 cut(s) 109, 178, 203
BanII GRGCYC 1 cut(s) 161
BbsI GAAGAC 1 cut(s) 3
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 2 cut(s) 199, 307
BccI CCATC 1 cut(s) 34
BceAI ACGGC 1 cut(s) 125
BciT130I CCWGG 3 cut(s) 155, 201, 207
BfaI CTAG 1 cut(s) 306
BisI GCNGC 2 cut(s) 188, 296
BlsI GCNGC 2 cut(s) 189, 297
Bme1390I CCNGG 3 cut(s) 155, 201, 207
Bme18I GGWCC 3 cut(s) 109, 178, 203
BmgT120I GGNCC 3 cut(s) 109, 178, 203
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 3 cut(s) 155, 201, 207
BmsI GCATC 1 cut(s) 73
BpiI GAAGAC 1 cut(s) 3
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 154
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 200, 206, 236
Bse1I ACTGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 139
BseBI CCWGG 3 cut(s) 155, 201, 207
BseDI CCNNGG 1 cut(s) 154
BseLI CCNNNNNNNGG 4 cut(s) 175, 200, 206, 236
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 254
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 2 cut(s) 199, 307
BseYI CCCAGC 1 cut(s) 229
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 2 cut(s) 244, 290
BsiHKAI GWGCWC 1 cut(s) 214
BslI CCNNNNNNNGG 4 cut(s) 175, 200, 206, 236
BsnI GGCC 2 cut(s) 244, 290
Bsp1286I GDGCHC 2 cut(s) 161, 214
Bsp143I GATC 2 cut(s) 73, 163
BspACI CCGC 1 cut(s) 112
BspANI GGCC 2 cut(s) 244, 290
BspCNI CTCAG 1 cut(s) 265
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 1 cut(s) 81
BsrDI GCAATG 1 cut(s) 139
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 2 cut(s) 73, 163
BssNI GRCGYC 1 cut(s) 44
Bst2UI CCWGG 3 cut(s) 155, 201, 207
Bst4CI ACNGT 1 cut(s) 34
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 1 cut(s) 44
BstAPI GCANNNNNTGC 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 288
BstDEI CTNAG 2 cut(s) 273, 414
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 2 cut(s) 76, 166
BstMBI GATC 2 cut(s) 73, 163
BstMWI GCNNNNNNNGC 5 cut(s) 196, 230, 250, 292, 395
BstNI CCWGG 3 cut(s) 155, 201, 207
BstSCI CCNGG 3 cut(s) 153, 199, 205
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 199, 307
BstV2I GAAGAC 1 cut(s) 3
BstX2I RGATCY 1 cut(s) 73
BstXI CCANNNNNNTGG 1 cut(s) 128
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 2 cut(s) 244, 290
BtgZI GCGATG 2 cut(s) 53, 129
BtsIMutI CAGTG 1 cut(s) 365
Cac8I GCNNGC 1 cut(s) 288
CfoI GCGC 1 cut(s) 187
Cfr13I GGNCC 3 cut(s) 109, 178, 203
CseI GACGC 1 cut(s) 33
Csp6I GTAC 3 cut(s) 311, 348, 411
CviAII CATG 2 cut(s) 196, 220
CviJI RGCY 8 cut(s) 101, 140, 159, 233, 244, 253, 290, 398
CviKI_1 RGCY 8 cut(s) 101, 140, 159, 233, 244, 253, 290, 398
CviQI GTAC 3 cut(s) 311, 348, 411
DdeI CTNAG 2 cut(s) 273, 414
DpnI GATC 2 cut(s) 75, 165
DpnII GATC 2 cut(s) 73, 163
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 161
Eco47I GGWCC 3 cut(s) 109, 178, 203
EcoO109I RGGNCCY 1 cut(s) 178
EcoRII CCWGG 3 cut(s) 153, 199, 205
EcoT38I GRGCYC 1 cut(s) 161
FaeI CATG 2 cut(s) 199, 223
FaiI YATR 3 cut(s) 197, 221, 270
FalI AAGNNNNNCTT 1 cut(s) 397
FatI CATG 2 cut(s) 195, 219
Fnu4HI GCNGC 2 cut(s) 188, 296
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 2 cut(s) 188, 296
FspBI CTAG 1 cut(s) 306
FspI TGCGCA 1 cut(s) 186
GlaI GCGC 1 cut(s) 186
GluI GCNGC 2 cut(s) 188, 296
GsaI CCCAGC 1 cut(s) 233
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 2 cut(s) 244, 290
HgaI GACGC 1 cut(s) 33
HhaI GCGC 1 cut(s) 187
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 2 cut(s) 199, 223
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 1 cut(s) 19
HpaI GTTAAC 1 cut(s) 361
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 3 cut(s) 30, 95, 163
Hpy8I GTNNAC 1 cut(s) 361
Hpy99I CGWCG 3 cut(s) 34, 49, 146
HpyAV CCTTC 2 cut(s) 234, 273
HpyCH4III ACNGT 1 cut(s) 34
HpyF10VI GCNNNNNNNGC 5 cut(s) 196, 230, 250, 292, 395
HpyF3I CTNAG 2 cut(s) 273, 414
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 2 cut(s) 199, 223
HspAI GCGC 1 cut(s) 185
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 2 cut(s) 73, 163
LmnI GCTCC 2 cut(s) 164, 265
Lsp1109I GCAGC 2 cut(s) 199, 307
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 306
MalI GATC 2 cut(s) 75, 165
MboI GATC 2 cut(s) 73, 163
MboII GAAGA 3 cut(s) 3, 70, 142
MfeI CAATTG 1 cut(s) 341
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 2 cut(s) 161, 214
MluCI AATT 2 cut(s) 317, 341
MmeI TCCRAC 2 cut(s) 53, 118
MnlI CCTC 5 cut(s) 230, 268, 346, 349, 401
MseI TTAA 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 233
MspR9I CCNGG 3 cut(s) 155, 201, 207
MunI CAATTG 1 cut(s) 341
MvaI CCWGG 3 cut(s) 155, 201, 207
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 5 cut(s) 196, 230, 250, 292, 395
NdeII GATC 2 cut(s) 73, 163
NlaIII CATG 2 cut(s) 199, 223
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 193
NsbI TGCGCA 1 cut(s) 186
PfeI GAWTC 1 cut(s) 19
PkrI GCNGC 2 cut(s) 189, 297
PpuMI RGGWCCY 1 cut(s) 178
Psp5II RGGWCCY 1 cut(s) 178
Psp6I CCWGG 3 cut(s) 153, 199, 205
PspFI CCCAGC 1 cut(s) 229
PspGI CCWGG 3 cut(s) 153, 199, 205
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 3 cut(s) 109, 178, 203
PspPPI RGGWCCY 1 cut(s) 178
PsuI RGATCY 1 cut(s) 73
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 3 cut(s) 312, 349, 412
RsaNI GTAC 3 cut(s) 311, 348, 411
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 2 cut(s) 188, 296
Sau3AI GATC 2 cut(s) 73, 163
Sau96I GGNCC 3 cut(s) 109, 178, 203
ScrFI CCNGG 3 cut(s) 155, 201, 207
SduI GDGCHC 2 cut(s) 161, 214
SetI ASST 6 cut(s) 180, 235, 307, 360, 400, 412
SfaNI GCATC 1 cut(s) 73
SinI GGWCC 3 cut(s) 109, 178, 203
Sse9I AATT 2 cut(s) 317, 341
SsiI CCGC 1 cut(s) 112
SspMI CTAG 1 cut(s) 306
StyD4I CCNGG 3 cut(s) 153, 199, 205
TaaI ACNGT 1 cut(s) 34
TaqI TCGA 1 cut(s) 81
TasI AATT 2 cut(s) 317, 341
TatI WGTACW 1 cut(s) 347
TfiI GAWTC 1 cut(s) 19
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 1 cut(s) 372
TseI GCWGC 2 cut(s) 187, 295
TspDTI ATGAA 1 cut(s) 150
TspRI CASTG 1 cut(s) 372
VpaK11BI GGWCC 3 cut(s) 109, 178, 203
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.