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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 102 |
| AfaI | GTAC | 1 cut(s) | 547 |
| AgsI | TTSAA | 1 cut(s) | 89 |
| AluBI | AGCT | 1 cut(s) | 164 |
| AluI | AGCT | 1 cut(s) | 164 |
| Alw21I | GWGCWC | 1 cut(s) | 194 |
| Alw26I | GTCTC | 1 cut(s) | 199 |
| AoxI | GGCC | 2 cut(s) | 19, 373 |
| ApeKI | GCWGC | 1 cut(s) | 485 |
| AsuHPI | GGTGA | 1 cut(s) | 131 |
| BauI | CACGAG | 1 cut(s) | 439 |
| Bbv12I | GWGCWC | 1 cut(s) | 194 |
| BbvI | GCAGC | 1 cut(s) | 497 |
| BcoDI | GTCTC | 1 cut(s) | 199 |
| BfmI | CTRYAG | 1 cut(s) | 102 |
| BfuAI | ACCTGC | 1 cut(s) | 102 |
| BisI | GCNGC | 1 cut(s) | 486 |
| BlsI | GCNGC | 1 cut(s) | 487 |
| Bse1I | ACTGG | 1 cut(s) | 149 |
| BseGI | GGATG | 2 cut(s) | 256, 280 |
| BseMII | CTCAG | 1 cut(s) | 30 |
| BseNI | ACTGG | 1 cut(s) | 149 |
| BseRI | GAGGAG | 3 cut(s) | 260, 341, 344 |
| BseXI | GCAGC | 1 cut(s) | 497 |
| BsgI | GTGCAG | 4 cut(s) | 471, 504, 534, 562 |
| BshFI | GGCC | 2 cut(s) | 21, 375 |
| BsiHKAI | GWGCWC | 1 cut(s) | 194 |
| BsiSI | CCGG | 1 cut(s) | 167 |
| BsmAI | GTCTC | 1 cut(s) | 199 |
| BsnI | GGCC | 2 cut(s) | 21, 375 |
| Bsp1286I | GDGCHC | 1 cut(s) | 194 |
| BspANI | GGCC | 2 cut(s) | 21, 375 |
| BspCNI | CTCAG | 1 cut(s) | 29 |
| BspMI | ACCTGC | 1 cut(s) | 102 |
| BsrI | ACTGG | 1 cut(s) | 149 |
| BssSI | CACGAG | 1 cut(s) | 439 |
| Bst2BI | CACGAG | 1 cut(s) | 439 |
| Bst6I | CTCTTC | 5 cut(s) | 237, 264, 303, 318, 336 |
| BstC8I | GCNNGC | 2 cut(s) | 99, 490 |
| BstDEI | CTNAG | 1 cut(s) | 16 |
| BstF5I | GGATG | 2 cut(s) | 256, 280 |
| BstMAI | GTCTC | 1 cut(s) | 199 |
| BstNSI | RCATGY | 2 cut(s) | 53, 174 |
| BstSFI | CTRYAG | 1 cut(s) | 102 |
| BstV1I | GCAGC | 1 cut(s) | 497 |
| BsuRI | GGCC | 2 cut(s) | 21, 375 |
| BtgZI | GCGATG | 1 cut(s) | 546 |
| BtsCI | GGATG | 2 cut(s) | 256, 280 |
| BtsIMutI | CAGTG | 1 cut(s) | 156 |
| BveI | ACCTGC | 1 cut(s) | 102 |
| Cac8I | GCNNGC | 2 cut(s) | 99, 490 |
| Csp6I | GTAC | 1 cut(s) | 546 |
| CviAII | CATG | 3 cut(s) | 50, 171, 472 |
| CviJI | RGCY | 3 cut(s) | 21, 164, 375 |
| CviKI_1 | RGCY | 3 cut(s) | 21, 164, 375 |
| CviQI | GTAC | 1 cut(s) | 546 |
| DdeI | CTNAG | 1 cut(s) | 16 |
| Eam1104I | CTCTTC | 5 cut(s) | 237, 264, 303, 318, 336 |
| EarI | CTCTTC | 5 cut(s) | 237, 264, 303, 318, 336 |
| FaeI | CATG | 3 cut(s) | 53, 174, 475 |
| FatI | CATG | 3 cut(s) | 49, 170, 471 |
| Fnu4HI | GCNGC | 1 cut(s) | 486 |
| FokI | GGATG | 2 cut(s) | 263, 287 |
| Fsp4HI | GCNGC | 1 cut(s) | 486 |
| GluI | GCNGC | 1 cut(s) | 486 |
| HaeIII | GGCC | 2 cut(s) | 21, 375 |
| HapII | CCGG | 1 cut(s) | 167 |
| Hin1II | CATG | 3 cut(s) | 53, 174, 475 |
| HpaII | CCGG | 1 cut(s) | 167 |
| HphI | GGTGA | 1 cut(s) | 131 |
| Hpy166II | GTNNAC | 1 cut(s) | 557 |
| Hpy188I | TCNGA | 1 cut(s) | 189 |
| Hpy8I | GTNNAC | 1 cut(s) | 557 |
| HpyAV | CCTTC | 1 cut(s) | 283 |
| HpyCH4V | TGCA | 7 cut(s) | 112, 405, 452, 471, 485, 515, 543 |
| HpyF3I | CTNAG | 1 cut(s) | 16 |
| Hsp92II | CATG | 3 cut(s) | 53, 174, 475 |
| LmnI | GCTCC | 1 cut(s) | 169 |
| LpnPI | CCDG | 9 cut(s) | 3, 107, 162, 180, 227, 389, 486, 507, 562 |
| Lsp1109I | GCAGC | 1 cut(s) | 497 |
| MhlI | GDGCHC | 1 cut(s) | 194 |
| MnlI | CCTC | 9 cut(s) | 232, 238, 241, 256, 265, 304, 319, 322, 325 |
| MseI | TTAA | 2 cut(s) | 54, 527 |
| MslI | CAYNNNNRTG | 2 cut(s) | 43, 476 |
| MspI | CCGG | 1 cut(s) | 167 |
| NlaIII | CATG | 3 cut(s) | 53, 174, 475 |
| NspI | RCATGY | 2 cut(s) | 53, 174 |
| PkrI | GCNGC | 1 cut(s) | 487 |
| RsaI | GTAC | 1 cut(s) | 547 |
| RsaNI | GTAC | 1 cut(s) | 546 |
| RseI | CAYNNNNRTG | 2 cut(s) | 43, 476 |
| SaqAI | TTAA | 2 cut(s) | 54, 527 |
| SatI | GCNGC | 1 cut(s) | 486 |
| SduI | GDGCHC | 1 cut(s) | 194 |
| SetI | ASST | 2 cut(s) | 96, 166 |
| SfcI | CTRYAG | 1 cut(s) | 102 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 43, 476 |
| TaqI | TCGA | 1 cut(s) | 139 |
| Tru1I | TTAA | 2 cut(s) | 54, 527 |
| Tru9I | TTAA | 2 cut(s) | 54, 527 |
| TscAI | CASTG | 1 cut(s) | 156 |
| TseI | GCWGC | 1 cut(s) | 485 |
| TspDTI | ATGAA | 3 cut(s) | 267, 291, 333 |
| TspRI | CASTG | 1 cut(s) | 156 |
| XceI | RCATGY | 2 cut(s) | 53, 174 |