MD05G1137100.v1.1

Epidermal growth factor receptor substrate 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001694
Physical Location & Seq
Forward (+)
26523981 .. 26524936
956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1137100.v1.1.491

Sequence Viewer

Length: 249 bp
ATGCCTGGTCCTGGTGCTCGACACATGGCGCCCCCAGCTGGAGGAAGGCCACCGAAGCCAGTTGCTCCTTCTCATGCTGTGGAAAGGCAGCAGGCCAATCAGCAGAAACCCAGAGTACCAGAATTGGAGAAACATCTTGTAAATCAACTGAGTACAGATGAGGTTAACTCGCTGAATTCAAAGTTCAAAGAAGCAACCGAAGTTGATAAAAAAGGTAGAAGAACTGGAGAAAATAATTTTGGATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_4 EF-hand_5

Protein Analysis

83

Amino Acids

8.99

Weight (kDa)

9.86

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 28
AcsI RAATTY 1 cut(s) 175
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 2 cut(s) 117, 154
AfiI CCNNNNNNNGG 3 cut(s) 11, 38, 41
AgsI TTSAA 2 cut(s) 180, 187
AjnI CCWGG 2 cut(s) 4, 10
AjuI GAANNNNNNNTTGG 1 cut(s) 222
AluBI AGCT 1 cut(s) 38
AluI AGCT 1 cut(s) 38
Alw21I GWGCWC 1 cut(s) 19
AoxI GGCC 2 cut(s) 47, 93
ApeKI GCWGC 1 cut(s) 88
ApoI RAATTY 1 cut(s) 175
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 8
AvaII GGWCC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 100
BciT130I CCWGG 2 cut(s) 6, 12
BfaI CTAG 1 cut(s) 247
BfoI RGCGCY 1 cut(s) 32
BisI GCNGC 1 cut(s) 89
BlsI GCNGC 1 cut(s) 90
Bme1390I CCNGG 2 cut(s) 6, 12
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 6, 12
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BpmI CTGGAG 2 cut(s) 60, 246
BsaHI GRCGYC 1 cut(s) 29
Bsc4I CCNNNNNNNGG 3 cut(s) 11, 38, 41
Bse1I ACTGG 2 cut(s) 59, 229
BseBI CCWGG 2 cut(s) 6, 12
BseLI CCNNNNNNNGG 3 cut(s) 11, 38, 41
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 2 cut(s) 59, 229
BseXI GCAGC 1 cut(s) 100
BseYI CCCAGC 1 cut(s) 34
BshFI GGCC 2 cut(s) 49, 95
BshNI GGYRCC 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 19
BslI CCNNNNNNNGG 3 cut(s) 11, 38, 41
BsnI GGCC 2 cut(s) 49, 95
Bsp1286I GDGCHC 1 cut(s) 19
BspANI GGCC 2 cut(s) 49, 95
BspCNI CTCAG 1 cut(s) 141
BspLI GGNNCC 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 28
BsrI ACTGG 2 cut(s) 59, 229
BssNI GRCGYC 1 cut(s) 29
Bst2UI CCWGG 2 cut(s) 6, 12
BstACI GRCGYC 1 cut(s) 29
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 1 cut(s) 149
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 1 cut(s) 31
BstMWI GCNNNNNNNGC 2 cut(s) 35, 55
BstNI CCWGG 2 cut(s) 6, 12
BstSCI CCNGG 2 cut(s) 4, 10
BstV1I GCAGC 1 cut(s) 100
BsuRI GGCC 2 cut(s) 49, 95
Cac8I GCNNGC 1 cut(s) 93
CfoI GCGC 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 8
Csp6I GTAC 2 cut(s) 116, 153
CviAII CATG 2 cut(s) 25, 74
CviJI RGCY 4 cut(s) 38, 49, 58, 95
CviKI_1 RGCY 4 cut(s) 38, 49, 58, 95
CviQI GTAC 2 cut(s) 116, 153
DdeI CTNAG 1 cut(s) 149
DinI GGCGCC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 8
EcoRI GAATTC 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 4, 10
EgeI GGCGCC 1 cut(s) 30
EheI GGCGCC 1 cut(s) 30
FaeI CATG 2 cut(s) 28, 77
FaiI YATR 2 cut(s) 26, 75
FatI CATG 2 cut(s) 24, 73
Fnu4HI GCNGC 1 cut(s) 89
Fsp4HI GCNGC 1 cut(s) 89
FspBI CTAG 1 cut(s) 247
GlaI GCGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 89
GsaI CCCAGC 1 cut(s) 38
GsuI CTGGAG 2 cut(s) 60, 246
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 2 cut(s) 49, 95
HhaI GCGC 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 2 cut(s) 28, 77
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 1 cut(s) 243
HpaI GTTAAC 1 cut(s) 166
Hpy166II GTNNAC 1 cut(s) 166
Hpy8I GTNNAC 1 cut(s) 166
HpyAV CCTTC 2 cut(s) 39, 78
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 55
HpyF3I CTNAG 1 cut(s) 149
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 2 cut(s) 28, 77
HspAI GCGC 1 cut(s) 29
KasI GGCGCC 1 cut(s) 28
KspAI GTTAAC 1 cut(s) 166
LmnI GCTCC 1 cut(s) 70
LpnPI CCDG 9 cut(s) 18, 24, 24, 48, 72, 77, 124, 132, 210
Lsp1109I GCAGC 1 cut(s) 100
MaeI CTAG 1 cut(s) 247
MboII GAAGA 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 3 cut(s) 122, 175, 235
Mly113I GGCGCC 1 cut(s) 29
MnlI CCTC 2 cut(s) 35, 154
MseI TTAA 1 cut(s) 165
MspA1I CMGCKG 1 cut(s) 38
MspR9I CCNGG 2 cut(s) 6, 12
MvaI CCWGG 2 cut(s) 6, 12
MwoI GCNNNNNNNGC 2 cut(s) 35, 55
NarI GGCGCC 1 cut(s) 29
NlaIII CATG 2 cut(s) 28, 77
NlaIV GGNNCC 1 cut(s) 30
PfeI GAWTC 1 cut(s) 243
PkrI GCNGC 1 cut(s) 90
PluTI GGCGCC 1 cut(s) 32
Psp6I CCWGG 2 cut(s) 4, 10
PspFI CCCAGC 1 cut(s) 34
PspGI CCWGG 2 cut(s) 4, 10
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 8
PvuII CAGCTG 1 cut(s) 38
RsaI GTAC 2 cut(s) 117, 154
RsaNI GTAC 2 cut(s) 116, 153
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 1 cut(s) 89
Sau96I GGNCC 1 cut(s) 8
ScrFI CCNGG 2 cut(s) 6, 12
SduI GDGCHC 1 cut(s) 19
SetI ASST 3 cut(s) 40, 165, 217
SfoI GGCGCC 1 cut(s) 30
SinI GGWCC 1 cut(s) 8
Sse9I AATT 3 cut(s) 122, 175, 235
SspDI GGCGCC 1 cut(s) 28
SspMI CTAG 1 cut(s) 247
StyD4I CCNGG 2 cut(s) 4, 10
TaqI TCGA 1 cut(s) 19
TasI AATT 3 cut(s) 122, 175, 235
TatI WGTACW 1 cut(s) 152
TfiI GAWTC 1 cut(s) 243
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TseI GCWGC 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 8
XapI RAATTY 1 cut(s) 175
XspI CTAG 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.