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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 13 |
| AfaI | GTAC | 1 cut(s) | 115 |
| AluBI | AGCT | 2 cut(s) | 9, 86 |
| AluI | AGCT | 2 cut(s) | 9, 86 |
| ApoI | RAATTY | 1 cut(s) | 13 |
| AsuHPI | GGTGA | 1 cut(s) | 62 |
| BccI | CCATC | 1 cut(s) | 47 |
| BlpI | GCTNAGC | 1 cut(s) | 5 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 5 |
| Bse118I | RCCGGY | 1 cut(s) | 70 |
| BsiSI | CCGG | 2 cut(s) | 71, 89 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 5 |
| BsrFI | RCCGGY | 1 cut(s) | 70 |
| BssAI | RCCGGY | 1 cut(s) | 70 |
| BstDEI | CTNAG | 1 cut(s) | 5 |
| Cfr10I | RCCGGY | 1 cut(s) | 70 |
| Csp6I | GTAC | 1 cut(s) | 114 |
| CviJI | RGCY | 3 cut(s) | 9, 20, 86 |
| CviKI_1 | RGCY | 3 cut(s) | 9, 20, 86 |
| CviQI | GTAC | 1 cut(s) | 114 |
| DdeI | CTNAG | 1 cut(s) | 5 |
| FaiI | YATR | 2 cut(s) | 94, 96 |
| HapII | CCGG | 2 cut(s) | 71, 89 |
| HpaII | CCGG | 2 cut(s) | 71, 89 |
| HphI | GGTGA | 1 cut(s) | 62 |
| HpyCH4V | TGCA | 1 cut(s) | 43 |
| HpyF3I | CTNAG | 1 cut(s) | 5 |
| LmnI | GCTCC | 1 cut(s) | 91 |
| LpnPI | CCDG | 2 cut(s) | 84, 102 |
| MboII | GAAGA | 1 cut(s) | 90 |
| MluCI | AATT | 1 cut(s) | 13 |
| MnlI | CCTC | 1 cut(s) | 22 |
| MspI | CCGG | 2 cut(s) | 71, 89 |
| RsaI | GTAC | 1 cut(s) | 115 |
| RsaNI | GTAC | 1 cut(s) | 114 |
| SetI | ASST | 4 cut(s) | 11, 33, 42, 88 |
| SgeI | CNNG | 7 cut(s) | 22, 72, 83, 87, 101, 123, 129 |
| Sse9I | AATT | 1 cut(s) | 13 |
| TaqI | TCGA | 2 cut(s) | 11, 33 |
| TasI | AATT | 1 cut(s) | 13 |
| TatI | WGTACW | 1 cut(s) | 113 |
| XapI | RAATTY | 1 cut(s) | 13 |