pycom05g14520

Epidermal growth factor receptor substrate 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001694
Physical Location & Seq
Forward (+)
18041049 .. 18041762
714 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g14520.1

Sequence Viewer

Length: 315 bp
ATGGCGTCGATGCAGAACCAATCGGCGAATGTGGATCTGTTCGATGCGTATTTCCGGCGGGCCGATTTGGACCGCGATGGCCGGATTAGTGGCAATGAAGCCGTCGCTTTCTTCCAGGGCTCTGGTCTCCCCAAACAGGTCCTTGCGCAGGATTGTTGGATTAAAAGTCCGAATATCTTCAGCTCCACTGGCTTCAGACCCATGTTCATCACTTTCATTTGCTTCAGGCTCATCTCCACCTTTGGAATCGTCATCTTGTCTTGTTTCTTCTTCTACTTTTGTTTCCCTCGGCATGAAGACATCGATCAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_4 EF-hand_5

Protein Analysis

105

Amino Acids

11.96

Weight (kDa)

5.62

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 147
AccII CGCG 1 cut(s) 75
AciI CCGC 2 cut(s) 58, 73
AclWI GGATC 1 cut(s) 42
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 1 cut(s) 309
AcuI CTGAAG 3 cut(s) 163, 178, 208
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 136, 148
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 1 cut(s) 183
AluI AGCT 1 cut(s) 183
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 42
AoxI GGCC 2 cut(s) 60, 79
ApoI RAATTY 1 cut(s) 309
Asp700I GAANNNNTTC 1 cut(s) 176
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 3 cut(s) 60, 70, 139
AvaII GGWCC 2 cut(s) 70, 139
BanII GRGCYC 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 303
BccI CCATC 1 cut(s) 71
BceAI ACGGC 1 cut(s) 86
BciT130I CCWGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 131
Bme1390I CCNGG 1 cut(s) 116
Bme18I GGWCC 2 cut(s) 70, 139
BmgT120I GGNCC 3 cut(s) 60, 70, 139
BmrFI CCNGG 1 cut(s) 116
BmsI GCATC 1 cut(s) 34
BpiI GAAGAC 1 cut(s) 303
Bsa29I ATCGAT 1 cut(s) 303
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 2 cut(s) 115, 287
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 148
Bse1I ACTGG 1 cut(s) 193
Bse3DI GCAATG 1 cut(s) 100
BseBI CCWGG 1 cut(s) 116
BseCI ATCGAT 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 115, 287
BseLI CCNNNNNNNGG 2 cut(s) 136, 148
BseMI GCAATG 1 cut(s) 100
BseNI ACTGG 1 cut(s) 193
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 2 cut(s) 62, 81
BshVI ATCGAT 1 cut(s) 303
BsiSI CCGG 2 cut(s) 55, 82
BslI CCNNNNNNNGG 2 cut(s) 136, 148
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 2 cut(s) 62, 81
Bso31I GGTCTC 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 122
Bsp143I GATC 2 cut(s) 34, 304
BspACI CCGC 2 cut(s) 58, 73
BspANI GGCC 2 cut(s) 62, 81
BspDI ATCGAT 1 cut(s) 303
BspFNI CGCG 1 cut(s) 75
BspPI GGATC 1 cut(s) 42
BspTNI GGTCTC 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 100
BsrI ACTGG 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 115, 287
BssMI GATC 2 cut(s) 34, 304
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 116
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 60
BstENI CCTNNNNNAGG 1 cut(s) 146
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 2 cut(s) 37, 307
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 2 cut(s) 34, 304
BstMWI GCNNNNNNNGC 1 cut(s) 189
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 114
BstUI CGCG 1 cut(s) 75
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 1 cut(s) 34
BstXI CCANNNNNNTGG 1 cut(s) 122
BstYI RGATCY 1 cut(s) 34
Bsu15I ATCGAT 1 cut(s) 303
BsuRI GGCC 2 cut(s) 62, 81
BsuTUI ATCGAT 1 cut(s) 303
BtgZI GCGATG 1 cut(s) 90
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 60
CfoI GCGC 1 cut(s) 148
Cfr13I GGNCC 3 cut(s) 60, 70, 139
ClaI ATCGAT 1 cut(s) 303
CviAII CATG 2 cut(s) 202, 293
CviJI RGCY 7 cut(s) 62, 81, 101, 120, 183, 192, 229
CviKI_1 RGCY 7 cut(s) 62, 81, 101, 120, 183, 192, 229
DpnI GATC 2 cut(s) 36, 306
DpnII GATC 2 cut(s) 34, 304
EaeI YGGCCR 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 122
Eco31I GGTCTC 1 cut(s) 131
Eco47I GGWCC 2 cut(s) 70, 139
Eco57I CTGAAG 3 cut(s) 163, 178, 208
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoO109I RGGNCCY 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 122
FaeI CATG 2 cut(s) 205, 296
FaiI YATR 2 cut(s) 203, 294
FatI CATG 2 cut(s) 201, 292
FauI CCCGC 1 cut(s) 51
FriOI GRGCYC 1 cut(s) 122
FspI TGCGCA 1 cut(s) 147
GlaI GCGC 1 cut(s) 147
HaeIII GGCC 2 cut(s) 62, 81
HapII CCGG 2 cut(s) 55, 82
HhaI GCGC 1 cut(s) 148
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 205, 296
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HinfI GANTC 1 cut(s) 246
HpaII CCGG 2 cut(s) 55, 82
Hpy188I TCNGA 2 cut(s) 171, 197
Hpy99I CGWCG 2 cut(s) 10, 107
HpyCH4V TGCA 1 cut(s) 13
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 205, 296
HspAI GCGC 1 cut(s) 146
Kzo9I GATC 2 cut(s) 34, 304
LmnI GCTCC 1 cut(s) 188
LpnPI CCDG 9 cut(s) 68, 95, 101, 108, 122, 128, 134, 174, 211
LweI GCATC 1 cut(s) 34
MalI GATC 2 cut(s) 36, 306
MboI GATC 2 cut(s) 34, 304
MboII GAAGA 5 cut(s) 103, 169, 259, 262, 308
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 122
MluCI AATT 1 cut(s) 309
MmeI TCCRAC 1 cut(s) 137
MnlI CCTC 1 cut(s) 297
MroXI GAANNNNTTC 1 cut(s) 176
MseI TTAA 1 cut(s) 162
MspI CCGG 2 cut(s) 55, 82
MspR9I CCNGG 1 cut(s) 116
MvaI CCWGG 1 cut(s) 116
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 1 cut(s) 189
NdeII GATC 2 cut(s) 34, 304
NlaIII CATG 2 cut(s) 205, 296
NmeAIII GCCGAG 1 cut(s) 268
NsbI TGCGCA 1 cut(s) 147
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 1 cut(s) 246
PpuMI RGGWCCY 1 cut(s) 139
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspPI GGNCC 3 cut(s) 60, 70, 139
PspPPI RGGWCCY 1 cut(s) 139
PsuI RGATCY 1 cut(s) 34
SaqAI TTAA 1 cut(s) 162
Sau3AI GATC 2 cut(s) 34, 304
Sau96I GGNCC 3 cut(s) 60, 70, 139
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 122
SetI ASST 3 cut(s) 141, 185, 242
SfaNI GCATC 1 cut(s) 34
SinI GGWCC 2 cut(s) 70, 139
Sse9I AATT 1 cut(s) 309
SsiI CCGC 2 cut(s) 58, 73
StyD4I CCNGG 1 cut(s) 114
TaqI TCGA 3 cut(s) 8, 42, 303
TasI AATT 1 cut(s) 309
TfiI GAWTC 1 cut(s) 246
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 1 cut(s) 193
TspDTI ATGAA 4 cut(s) 111, 196, 205, 309
TspRI CASTG 1 cut(s) 193
VpaK11BI GGWCC 2 cut(s) 70, 139
XagI CCTNNNNNAGG 1 cut(s) 146
XapI RAATTY 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.