pycom05g09410

Epidermal growth factor receptor substrate 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001694
Physical Location & Seq
Forward (+)
12582056 .. 12585366
3311 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g09410.3

Sequence Viewer

Length: 258 bp
ATGGCGTCGGCACAGAAACAATCCGCAAATGTAGATCTCTTCGATGCGTATTTCGGACAAGCGGATATGGACCGCGATGGCCGGATTAGTGGCAATGAAGCAGTCGCTTTCTTCCAGGGCTCAGGTCTCCCCAAACAGGATTTGTACAAGAGCAGATGTGACAATCGCCTTACTGAGATCACAGAAAGGGCCTCTGCTGATAAATATGAGGAGAAGAAAATGGAGCTAAACAGGGCAGTTGTCAAGATGGAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_4 EF-hand_5

Protein Analysis

86

Amino Acids

9.61

Weight (kDa)

5.09

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 75
AciI CCGC 3 cut(s) 24, 62, 73
AcoI YGGCCR 1 cut(s) 79
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 1 cut(s) 136
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 1 cut(s) 226
AluI AGCT 1 cut(s) 226
Alw26I GTCTC 1 cut(s) 131
AoxI GGCC 2 cut(s) 79, 189
AspS9I GGNCC 2 cut(s) 70, 189
AvaII GGWCC 1 cut(s) 70
BanII GRGCYC 1 cut(s) 122
BccI CCATC 2 cut(s) 71, 241
BciT130I CCWGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 131
BglII AGATCT 1 cut(s) 34
Bme1390I CCNGG 1 cut(s) 116
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 70, 189
BmrFI CCNGG 1 cut(s) 116
BmsI GCATC 1 cut(s) 34
Bpu10I CCTNAGC 1 cut(s) 121
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 115
Bsc4I CCNNNNNNNGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 100
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 136
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 2 cut(s) 135, 165
BseRI GAGGAG 1 cut(s) 224
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 2 cut(s) 81, 191
BsiSI CCGG 1 cut(s) 82
BslI CCNNNNNNNGG 1 cut(s) 136
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 2 cut(s) 81, 191
Bso31I GGTCTC 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 122
Bsp1407I TGTACA 1 cut(s) 144
Bsp143I GATC 2 cut(s) 34, 177
BspACI CCGC 3 cut(s) 24, 62, 73
BspANI GGCC 2 cut(s) 81, 191
BspCNI CTCAG 2 cut(s) 134, 166
BspFNI CGCG 1 cut(s) 75
BspTNI GGTCTC 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 100
BsrGI TGTACA 1 cut(s) 144
BssECI CCNNGG 1 cut(s) 115
BssMI GATC 2 cut(s) 34, 177
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 116
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 144
BstDEI CTNAG 2 cut(s) 121, 174
BstFNI CGCG 1 cut(s) 75
BstKTI GATC 2 cut(s) 37, 180
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 2 cut(s) 34, 177
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 114
BstUI CGCG 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuRI GGCC 2 cut(s) 81, 191
BtgZI GCGATG 1 cut(s) 90
Cfr13I GGNCC 2 cut(s) 70, 189
Csp6I GTAC 1 cut(s) 145
CviJI RGCY 4 cut(s) 81, 120, 191, 226
CviKI_1 RGCY 4 cut(s) 81, 120, 191, 226
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 2 cut(s) 121, 174
DpnI GATC 2 cut(s) 36, 179
DpnII GATC 2 cut(s) 34, 177
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco24I GRGCYC 1 cut(s) 122
Eco31I GGTCTC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 122
FaiI YATR 2 cut(s) 68, 207
FriOI GRGCYC 1 cut(s) 122
HaeIII GGCC 2 cut(s) 81, 191
HapII CCGG 1 cut(s) 82
Hin1I GRCGYC 1 cut(s) 5
HpaII CCGG 1 cut(s) 82
Hpy188I TCNGA 1 cut(s) 56
Hpy188III TCNNGA 1 cut(s) 244
Hpy99I CGWCG 1 cut(s) 10
HpyF3I CTNAG 2 cut(s) 121, 174
Hsp92I GRCGYC 1 cut(s) 5
Kzo9I GATC 2 cut(s) 34, 177
LmnI GCTCC 2 cut(s) 223, 250
LpnPI CCDG 6 cut(s) 95, 101, 108, 122, 128, 217
LweI GCATC 1 cut(s) 34
MaeIII GTNAC 1 cut(s) 158
MalI GATC 2 cut(s) 36, 179
MboI GATC 2 cut(s) 34, 177
MboII GAAGA 3 cut(s) 31, 103, 226
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 122
MnlI CCTC 2 cut(s) 202, 202
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 1 cut(s) 116
MvaI CCWGG 1 cut(s) 116
MvnI CGCG 1 cut(s) 75
NdeII GATC 2 cut(s) 34, 177
NmuCI GTSAC 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspPI GGNCC 2 cut(s) 70, 189
PsuI RGATCY 1 cut(s) 34
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
Sau3AI GATC 2 cut(s) 34, 177
Sau96I GGNCC 2 cut(s) 70, 189
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 122
SetI ASST 2 cut(s) 127, 228
SfaNI GCATC 1 cut(s) 34
SgeI CNNG 9 cut(s) 71, 86, 94, 127, 128, 135, 149, 160, 244
SinI GGWCC 1 cut(s) 70
SsiI CCGC 3 cut(s) 24, 62, 73
StyD4I CCNGG 1 cut(s) 114
TaqI TCGA 1 cut(s) 42
TatI WGTACW 1 cut(s) 144
TseFI GTSAC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 158
TspDTI ATGAA 1 cut(s) 111
VpaK11BI GGWCC 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.