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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 97 |
| Acc36I | ACCTGC | 1 cut(s) | 97 |
| AccII | CGCG | 1 cut(s) | 552 |
| AfaI | GTAC | 3 cut(s) | 452, 471, 535 |
| AjnI | CCWGG | 1 cut(s) | 205 |
| AluBI | AGCT | 1 cut(s) | 182 |
| AluI | AGCT | 1 cut(s) | 182 |
| Alw26I | GTCTC | 1 cut(s) | 217 |
| AoxI | GGCC | 1 cut(s) | 376 |
| ApeKI | GCWGC | 1 cut(s) | 506 |
| AspLEI | GCGC | 1 cut(s) | 515 |
| AsuHPI | GGTGA | 3 cut(s) | 35, 78, 149 |
| BauI | CACGAG | 1 cut(s) | 576 |
| BbvI | GCAGC | 1 cut(s) | 518 |
| BccI | CCATC | 1 cut(s) | 54 |
| BciT130I | CCWGG | 1 cut(s) | 207 |
| BcoDI | GTCTC | 1 cut(s) | 217 |
| BfuAI | ACCTGC | 1 cut(s) | 97 |
| BisI | GCNGC | 1 cut(s) | 507 |
| BlsI | GCNGC | 1 cut(s) | 508 |
| Bme1390I | CCNGG | 1 cut(s) | 207 |
| BmrFI | CCNGG | 1 cut(s) | 207 |
| BmsI | GCATC | 1 cut(s) | 23 |
| Bse1I | ACTGG | 1 cut(s) | 167 |
| BseBI | CCWGG | 1 cut(s) | 207 |
| BseGI | GGATG | 4 cut(s) | 46, 274, 298, 498 |
| BseMII | CTCAG | 1 cut(s) | 438 |
| BseNI | ACTGG | 1 cut(s) | 167 |
| BseRI | GAGGAG | 1 cut(s) | 278 |
| BseXI | GCAGC | 1 cut(s) | 518 |
| BsgI | GTGCAG | 4 cut(s) | 444, 497, 525, 583 |
| Bsh1236I | CGCG | 1 cut(s) | 552 |
| BshFI | GGCC | 1 cut(s) | 378 |
| BsiSI | CCGG | 1 cut(s) | 185 |
| BsmAI | GTCTC | 1 cut(s) | 217 |
| BsnI | GGCC | 1 cut(s) | 378 |
| BspANI | GGCC | 1 cut(s) | 378 |
| BspCNI | CTCAG | 1 cut(s) | 439 |
| BspFNI | CGCG | 1 cut(s) | 552 |
| BspMI | ACCTGC | 1 cut(s) | 97 |
| BsrI | ACTGG | 1 cut(s) | 167 |
| BssSI | CACGAG | 1 cut(s) | 576 |
| Bst2BI | CACGAG | 1 cut(s) | 576 |
| Bst2UI | CCWGG | 1 cut(s) | 207 |
| Bst4CI | ACNGT | 2 cut(s) | 81, 455 |
| Bst6I | CTCTTC | 4 cut(s) | 255, 282, 321, 339 |
| BstC8I | GCNNGC | 2 cut(s) | 6, 511 |
| BstDEI | CTNAG | 2 cut(s) | 191, 447 |
| BstF5I | GGATG | 4 cut(s) | 46, 274, 298, 498 |
| BstFNI | CGCG | 1 cut(s) | 552 |
| BstHHI | GCGC | 1 cut(s) | 515 |
| BstMAI | GTCTC | 1 cut(s) | 217 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 14, 512 |
| BstNI | CCWGG | 1 cut(s) | 207 |
| BstSCI | CCNGG | 1 cut(s) | 205 |
| BstUI | CGCG | 1 cut(s) | 552 |
| BstV1I | GCAGC | 1 cut(s) | 518 |
| BsuRI | GGCC | 1 cut(s) | 378 |
| BtgZI | GCGATG | 1 cut(s) | 567 |
| BtsCI | GGATG | 4 cut(s) | 46, 274, 298, 498 |
| BtsIMutI | CAGTG | 1 cut(s) | 174 |
| BveI | ACCTGC | 1 cut(s) | 97 |
| Cac8I | GCNNGC | 2 cut(s) | 6, 511 |
| CfoI | GCGC | 1 cut(s) | 515 |
| Csp6I | GTAC | 3 cut(s) | 451, 470, 534 |
| CviJI | RGCY | 3 cut(s) | 182, 378, 446 |
| CviKI_1 | RGCY | 3 cut(s) | 182, 378, 446 |
| CviQI | GTAC | 3 cut(s) | 451, 470, 534 |
| DdeI | CTNAG | 2 cut(s) | 191, 447 |
| Eam1104I | CTCTTC | 4 cut(s) | 255, 282, 321, 339 |
| EarI | CTCTTC | 4 cut(s) | 255, 282, 321, 339 |
| EcoRII | CCWGG | 1 cut(s) | 205 |
| FaiI | YATR | 5 cut(s) | 391, 413, 419, 500, 583 |
| Fnu4HI | GCNGC | 1 cut(s) | 507 |
| FokI | GGATG | 4 cut(s) | 33, 281, 305, 505 |
| Fsp4HI | GCNGC | 1 cut(s) | 507 |
| GlaI | GCGC | 1 cut(s) | 514 |
| GluI | GCNGC | 1 cut(s) | 507 |
| HaeIII | GGCC | 1 cut(s) | 378 |
| HapII | CCGG | 1 cut(s) | 185 |
| HhaI | GCGC | 1 cut(s) | 515 |
| Hin6I | GCGC | 1 cut(s) | 513 |
| HinP1I | GCGC | 1 cut(s) | 513 |
| HpaII | CCGG | 1 cut(s) | 185 |
| HphI | GGTGA | 3 cut(s) | 35, 78, 149 |
| HpyAV | CCTTC | 1 cut(s) | 301 |
| HpyCH4III | ACNGT | 2 cut(s) | 81, 455 |
| HpyCH4V | TGCA | 7 cut(s) | 8, 130, 408, 425, 478, 506, 564 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 14, 512 |
| HpyF3I | CTNAG | 2 cut(s) | 191, 447 |
| HspAI | GCGC | 1 cut(s) | 513 |
| LmnI | GCTCC | 2 cut(s) | 187, 356 |
| LpnPI | CCDG | 7 cut(s) | 102, 180, 192, 198, 219, 392, 507 |
| Lsp1109I | GCAGC | 1 cut(s) | 518 |
| LweI | GCATC | 1 cut(s) | 23 |
| MboII | GAAGA | 9 cut(s) | 186, 272, 284, 296, 299, 338, 350, 353, 356 |
| MluCI | AATT | 3 cut(s) | 123, 414, 434 |
| MnlI | CCTC | 7 cut(s) | 7, 250, 256, 259, 274, 283, 322 |
| MspI | CCGG | 1 cut(s) | 185 |
| MspR9I | CCNGG | 1 cut(s) | 207 |
| MvaI | CCWGG | 1 cut(s) | 207 |
| MvnI | CGCG | 1 cut(s) | 552 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 14, 512 |
| PaqCI | CACCTGC | 1 cut(s) | 97 |
| PkrI | GCNGC | 1 cut(s) | 508 |
| Psp6I | CCWGG | 1 cut(s) | 205 |
| PspGI | CCWGG | 1 cut(s) | 205 |
| RsaI | GTAC | 3 cut(s) | 452, 471, 535 |
| RsaNI | GTAC | 3 cut(s) | 451, 470, 534 |
| SatI | GCNGC | 1 cut(s) | 507 |
| ScrFI | CCNGG | 1 cut(s) | 207 |
| SetI | ASST | 4 cut(s) | 25, 91, 107, 184 |
| SfaNI | GCATC | 1 cut(s) | 23 |
| Sse9I | AATT | 3 cut(s) | 123, 414, 434 |
| StyD4I | CCNGG | 1 cut(s) | 205 |
| TaaI | ACNGT | 2 cut(s) | 81, 455 |
| TaqI | TCGA | 1 cut(s) | 157 |
| TasI | AATT | 3 cut(s) | 123, 414, 434 |
| TatI | WGTACW | 3 cut(s) | 450, 469, 533 |
| TscAI | CASTG | 1 cut(s) | 174 |
| TseI | GCWGC | 1 cut(s) | 506 |
| TspDTI | ATGAA | 3 cut(s) | 285, 309, 351 |
| TspRI | CASTG | 1 cut(s) | 174 |