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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 20 |
| AciI | CCGC | 1 cut(s) | 20 |
| AclWI | GGATC | 1 cut(s) | 50 |
| AcsI | RAATTY | 1 cut(s) | 122 |
| AfaI | GTAC | 1 cut(s) | 396 |
| AluBI | AGCT | 2 cut(s) | 53, 104 |
| AluI | AGCT | 2 cut(s) | 53, 104 |
| Alw26I | GTCTC | 1 cut(s) | 139 |
| AlwI | GGATC | 1 cut(s) | 50 |
| AoxI | GGCC | 1 cut(s) | 57 |
| ApoI | RAATTY | 1 cut(s) | 122 |
| AsuHPI | GGTGA | 1 cut(s) | 103 |
| AxyI | CCTNAGG | 1 cut(s) | 11 |
| BauI | CACGAG | 1 cut(s) | 390 |
| BbvCI | CCTCAGC | 1 cut(s) | 54 |
| BccI | CCATC | 2 cut(s) | 29, 68 |
| BceAI | ACGGC | 1 cut(s) | 44 |
| BcoDI | GTCTC | 1 cut(s) | 139 |
| Bpu10I | CCTNAGC | 1 cut(s) | 54 |
| Bse21I | CCTNAGG | 1 cut(s) | 11 |
| Bse3DI | GCAATG | 1 cut(s) | 373 |
| BseGI | GGATG | 1 cut(s) | 196 |
| BseMI | GCAATG | 1 cut(s) | 373 |
| BseMII | CTCAG | 1 cut(s) | 45 |
| BseRI | GAGGAG | 4 cut(s) | 200, 215, 269, 272 |
| BsgI | GTGCAG | 2 cut(s) | 369, 397 |
| BshFI | GGCC | 1 cut(s) | 59 |
| BsiSI | CCGG | 1 cut(s) | 107 |
| BsmAI | GTCTC | 1 cut(s) | 139 |
| BsnI | GGCC | 1 cut(s) | 59 |
| Bsp143I | GATC | 1 cut(s) | 42 |
| BspACI | CCGC | 1 cut(s) | 20 |
| BspANI | GGCC | 1 cut(s) | 59 |
| BspCNI | CTCAG | 1 cut(s) | 46 |
| BspPI | GGATC | 1 cut(s) | 50 |
| BsrBI | CCGCTC | 1 cut(s) | 20 |
| BsrDI | GCAATG | 1 cut(s) | 373 |
| BssMI | GATC | 1 cut(s) | 42 |
| BssSI | CACGAG | 1 cut(s) | 390 |
| Bst2BI | CACGAG | 1 cut(s) | 390 |
| Bst6I | CTCTTC | 4 cut(s) | 177, 192, 231, 264 |
| BstDEI | CTNAG | 2 cut(s) | 11, 54 |
| BstF5I | GGATG | 1 cut(s) | 196 |
| BstKTI | GATC | 1 cut(s) | 45 |
| BstMAI | GTCTC | 1 cut(s) | 139 |
| BstMBI | GATC | 1 cut(s) | 42 |
| BstNSI | RCATGY | 1 cut(s) | 114 |
| Bsu36I | CCTNAGG | 1 cut(s) | 11 |
| BsuRI | GGCC | 1 cut(s) | 59 |
| BtsCI | GGATG | 1 cut(s) | 196 |
| Csp6I | GTAC | 1 cut(s) | 395 |
| CviAII | CATG | 1 cut(s) | 111 |
| CviJI | RGCY | 4 cut(s) | 5, 53, 59, 104 |
| CviKI_1 | RGCY | 4 cut(s) | 5, 53, 59, 104 |
| CviQI | GTAC | 1 cut(s) | 395 |
| DdeI | CTNAG | 2 cut(s) | 11, 54 |
| DpnI | GATC | 1 cut(s) | 44 |
| DpnII | GATC | 1 cut(s) | 42 |
| Eam1104I | CTCTTC | 4 cut(s) | 177, 192, 231, 264 |
| EarI | CTCTTC | 4 cut(s) | 177, 192, 231, 264 |
| Eco81I | CCTNAGG | 1 cut(s) | 11 |
| FaeI | CATG | 1 cut(s) | 114 |
| FaiI | YATR | 4 cut(s) | 112, 316, 338, 344 |
| FatI | CATG | 1 cut(s) | 110 |
| FokI | GGATG | 1 cut(s) | 203 |
| HaeIII | GGCC | 1 cut(s) | 59 |
| HapII | CCGG | 1 cut(s) | 107 |
| Hin1II | CATG | 1 cut(s) | 114 |
| HpaII | CCGG | 1 cut(s) | 107 |
| HphI | GGTGA | 1 cut(s) | 103 |
| HpyAV | CCTTC | 1 cut(s) | 211 |
| HpyCH4V | TGCA | 5 cut(s) | 64, 333, 350, 378, 403 |
| HpyF3I | CTNAG | 2 cut(s) | 11, 54 |
| Hsp92II | CATG | 1 cut(s) | 114 |
| Kzo9I | GATC | 1 cut(s) | 42 |
| LmnI | GCTCC | 2 cut(s) | 109, 281 |
| LpnPI | CCDG | 3 cut(s) | 120, 317, 342 |
| MalI | GATC | 1 cut(s) | 44 |
| MbiI | CCGCTC | 1 cut(s) | 20 |
| MboI | GATC | 1 cut(s) | 42 |
| MboII | GAAGA | 9 cut(s) | 108, 191, 194, 206, 209, 218, 221, 248, 281 |
| MluCI | AATT | 1 cut(s) | 122 |
| MspI | CCGG | 1 cut(s) | 107 |
| NdeII | GATC | 1 cut(s) | 42 |
| NlaIII | CATG | 1 cut(s) | 114 |
| NspI | RCATGY | 1 cut(s) | 114 |
| RsaI | GTAC | 1 cut(s) | 396 |
| RsaNI | GTAC | 1 cut(s) | 395 |
| Sau3AI | GATC | 1 cut(s) | 42 |
| SetI | ASST | 3 cut(s) | 12, 55, 106 |
| Sse9I | AATT | 1 cut(s) | 122 |
| SsiI | CCGC | 1 cut(s) | 20 |
| TaqI | TCGA | 1 cut(s) | 372 |
| TasI | AATT | 1 cut(s) | 122 |
| TatI | WGTACW | 1 cut(s) | 394 |
| TspDTI | ATGAA | 2 cut(s) | 207, 261 |
| TspGWI | ACGGA | 1 cut(s) | 45 |
| XapI | RAATTY | 1 cut(s) | 122 |
| XceI | RCATGY | 1 cut(s) | 114 |