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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 102 |
| AfaI | GTAC | 1 cut(s) | 477 |
| AgsI | TTSAA | 1 cut(s) | 89 |
| AluBI | AGCT | 1 cut(s) | 164 |
| AluI | AGCT | 1 cut(s) | 164 |
| Alw26I | GTCTC | 1 cut(s) | 199 |
| AoxI | GGCC | 2 cut(s) | 19, 373 |
| ApeKI | GCWGC | 1 cut(s) | 422 |
| AsuHPI | GGTGA | 1 cut(s) | 131 |
| BbvI | GCAGC | 1 cut(s) | 434 |
| BcoDI | GTCTC | 1 cut(s) | 199 |
| BfmI | CTRYAG | 2 cut(s) | 102, 420 |
| BfuAI | ACCTGC | 1 cut(s) | 102 |
| BisI | GCNGC | 1 cut(s) | 423 |
| BlsI | GCNGC | 1 cut(s) | 424 |
| BsaAI | YACGTR | 1 cut(s) | 173 |
| Bse1I | ACTGG | 1 cut(s) | 149 |
| Bse3DI | GCAATG | 1 cut(s) | 454 |
| BseGI | GGATG | 2 cut(s) | 256, 280 |
| BseMI | GCAATG | 1 cut(s) | 454 |
| BseMII | CTCAG | 1 cut(s) | 30 |
| BseNI | ACTGG | 1 cut(s) | 149 |
| BseRI | GAGGAG | 3 cut(s) | 260, 341, 344 |
| BseXI | GCAGC | 1 cut(s) | 434 |
| BsgI | GTGCAG | 1 cut(s) | 478 |
| BshFI | GGCC | 2 cut(s) | 21, 375 |
| BsiSI | CCGG | 1 cut(s) | 167 |
| BsmAI | GTCTC | 1 cut(s) | 199 |
| BsnI | GGCC | 2 cut(s) | 21, 375 |
| BspANI | GGCC | 2 cut(s) | 21, 375 |
| BspCNI | CTCAG | 1 cut(s) | 29 |
| BspMAI | CTGCAG | 1 cut(s) | 424 |
| BspMI | ACCTGC | 1 cut(s) | 102 |
| BsrDI | GCAATG | 1 cut(s) | 454 |
| BsrI | ACTGG | 1 cut(s) | 149 |
| Bst6I | CTCTTC | 5 cut(s) | 237, 264, 303, 318, 336 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 428 |
| BstBAI | YACGTR | 1 cut(s) | 173 |
| BstC8I | GCNNGC | 1 cut(s) | 99 |
| BstDEI | CTNAG | 1 cut(s) | 16 |
| BstF5I | GGATG | 2 cut(s) | 256, 280 |
| BstMAI | GTCTC | 1 cut(s) | 199 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 428 |
| BstNSI | RCATGY | 1 cut(s) | 53 |
| BstSFI | CTRYAG | 2 cut(s) | 102, 420 |
| BstV1I | GCAGC | 1 cut(s) | 434 |
| BsuRI | GGCC | 2 cut(s) | 21, 375 |
| BtsCI | GGATG | 2 cut(s) | 256, 280 |
| BtsIMutI | CAGTG | 1 cut(s) | 156 |
| BveI | ACCTGC | 1 cut(s) | 102 |
| Cac8I | GCNNGC | 1 cut(s) | 99 |
| Csp6I | GTAC | 1 cut(s) | 476 |
| CviAII | CATG | 1 cut(s) | 50 |
| CviJI | RGCY | 3 cut(s) | 21, 164, 375 |
| CviKI_1 | RGCY | 3 cut(s) | 21, 164, 375 |
| CviQI | GTAC | 1 cut(s) | 476 |
| DdeI | CTNAG | 1 cut(s) | 16 |
| Eam1104I | CTCTTC | 5 cut(s) | 237, 264, 303, 318, 336 |
| EarI | CTCTTC | 5 cut(s) | 237, 264, 303, 318, 336 |
| FaeI | CATG | 1 cut(s) | 53 |
| FaiI | YATR | 5 cut(s) | 51, 104, 388, 410, 416 |
| FatI | CATG | 1 cut(s) | 49 |
| Fnu4HI | GCNGC | 1 cut(s) | 423 |
| FokI | GGATG | 2 cut(s) | 263, 287 |
| Fsp4HI | GCNGC | 1 cut(s) | 423 |
| GluI | GCNGC | 1 cut(s) | 423 |
| HaeIII | GGCC | 2 cut(s) | 21, 375 |
| HapII | CCGG | 1 cut(s) | 167 |
| Hin1II | CATG | 1 cut(s) | 53 |
| HpaII | CCGG | 1 cut(s) | 167 |
| HphI | GGTGA | 1 cut(s) | 131 |
| HpyAV | CCTTC | 1 cut(s) | 283 |
| HpyCH4IV | ACGT | 1 cut(s) | 172 |
| HpyCH4V | TGCA | 6 cut(s) | 112, 405, 422, 431, 459, 484 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 428 |
| HpyF3I | CTNAG | 1 cut(s) | 16 |
| HpySE526I | ACGT | 1 cut(s) | 172 |
| Hsp92II | CATG | 1 cut(s) | 53 |
| LmnI | GCTCC | 1 cut(s) | 169 |
| LpnPI | CCDG | 5 cut(s) | 3, 107, 162, 180, 389 |
| Lsp1109I | GCAGC | 1 cut(s) | 434 |
| MaeII | ACGT | 1 cut(s) | 172 |
| MnlI | CCTC | 9 cut(s) | 232, 238, 241, 256, 265, 304, 319, 322, 325 |
| MseI | TTAA | 1 cut(s) | 54 |
| MslI | CAYNNNNRTG | 1 cut(s) | 43 |
| MspI | CCGG | 1 cut(s) | 167 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 428 |
| NlaIII | CATG | 1 cut(s) | 53 |
| NspI | RCATGY | 1 cut(s) | 53 |
| PkrI | GCNGC | 1 cut(s) | 424 |
| Ppu21I | YACGTR | 1 cut(s) | 173 |
| PstI | CTGCAG | 1 cut(s) | 424 |
| RsaI | GTAC | 1 cut(s) | 477 |
| RsaNI | GTAC | 1 cut(s) | 476 |
| RseI | CAYNNNNRTG | 1 cut(s) | 43 |
| SaqAI | TTAA | 1 cut(s) | 54 |
| SatI | GCNGC | 1 cut(s) | 423 |
| SetI | ASST | 3 cut(s) | 96, 166, 175 |
| SfcI | CTRYAG | 2 cut(s) | 102, 420 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 43 |
| TaiI | ACGT | 1 cut(s) | 175 |
| TaqI | TCGA | 2 cut(s) | 139, 453 |
| TatI | WGTACW | 1 cut(s) | 475 |
| Tru1I | TTAA | 1 cut(s) | 54 |
| Tru9I | TTAA | 1 cut(s) | 54 |
| TscAI | CASTG | 1 cut(s) | 156 |
| TseI | GCWGC | 1 cut(s) | 422 |
| TspDTI | ATGAA | 3 cut(s) | 267, 291, 333 |
| TspRI | CASTG | 1 cut(s) | 156 |
| XceI | RCATGY | 1 cut(s) | 53 |