Rroxscaffold_2G00104930

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001694
Physical Location & Seq
Reverse (-)
28322406 .. 28334860
12455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00104930.1

Sequence Viewer

Length: 453 bp
ATGCACCGTGTGAGCAGGAGGGGGACTAGGCGCGGATGGAGGATAGACCAAAGGCCGGGAGGCTCCGGTGAGGAGGCTGGATCAAAGGCCGGAATGCAGGCGGTTGAGATCGGTGCTGCAAGTGGTTGGGTAGTGGTAAACAATTCTGGTGTGGAAGAAAACAAGGAAAAGACAAGTGGTTATCTAAAGCTTGACTTTTCAAGGCCTATTGGCAATGACGTAATTAGCATTTATTTGGGAACAATCTTCTTGCACGGAAAGGAGAGGGCTGGAAATCCCGAGACATTGAGTCTAAAGCTAGGAAGTGACATTATTTTAACTGAAAAGGTTCCAGATGCAACTAGTGACCATGAAATTCGGAGAGAGTCCGTAGGAGAAGAAAGGAATCCAGATTCAAGCAGTGTGATAGAGCAATGGGCTGAGTTTCAATATGTTATTGCATATTTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.55

Weight (kDa)

5.67

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 33
AciI CCGC 2 cut(s) 33, 101
AclWI GGATC 1 cut(s) 88
AcsI RAATTY 1 cut(s) 354
AdeI CACNNNGTG 1 cut(s) 10
AfiI CCNNNNNNNGG 1 cut(s) 55
AgsI TTSAA 3 cut(s) 201, 396, 428
AhdI GACNNNNNGTC 1 cut(s) 288
AhlI ACTAGT 1 cut(s) 341
AluBI AGCT 2 cut(s) 190, 298
AluI AGCT 2 cut(s) 190, 298
Alw26I GTCTC 1 cut(s) 275
AlwI GGATC 1 cut(s) 88
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 3 cut(s) 53, 87, 203
ApeKI GCWGC 1 cut(s) 116
ApoI RAATTY 1 cut(s) 354
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 1 cut(s) 33
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 278
BbvI GCAGC 1 cut(s) 103
BccI CCATC 1 cut(s) 30
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 275
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 3 cut(s) 27, 299, 342
BisI GCNGC 1 cut(s) 117
BlsI GCNGC 1 cut(s) 118
Bme1390I CCNGG 1 cut(s) 57
BmeRI GACNNNNNGTC 1 cut(s) 288
BmeT110I CYCGRG 1 cut(s) 278
BmiI GGNNCC 2 cut(s) 64, 330
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 1 cut(s) 325
BpuMI CCSGG 1 cut(s) 57
BsaWI WCCGGW 1 cut(s) 65
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse3DI GCAATG 2 cut(s) 220, 419
BseGI GGATG 1 cut(s) 41
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMI GCAATG 2 cut(s) 220, 419
BseMII CTCAG 1 cut(s) 411
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 1 cut(s) 103
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 3 cut(s) 55, 89, 205
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 3 cut(s) 56, 66, 90
BslFI GGGAC 1 cut(s) 37
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 37
BsmI GAATGC 1 cut(s) 99
BsnI GGCC 3 cut(s) 55, 89, 205
BsoBI CYCGRG 1 cut(s) 278
Bsp143I GATC 2 cut(s) 80, 108
BspACI CCGC 2 cut(s) 33, 101
BspANI GGCC 3 cut(s) 55, 89, 205
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 64, 330
BspPI GGATC 1 cut(s) 88
BsrDI GCAATG 2 cut(s) 220, 419
BssMI GATC 2 cut(s) 80, 108
Bst4CI ACNGT 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 1 cut(s) 420
BstF5I GGATG 1 cut(s) 41
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 2 cut(s) 83, 111
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 2 cut(s) 80, 108
BstSCI CCNGG 1 cut(s) 55
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 103
BsuRI GGCC 3 cut(s) 55, 89, 205
BtsCI GGATG 1 cut(s) 41
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 1 cut(s) 99
CfoI GCGC 1 cut(s) 33
CviAII CATG 1 cut(s) 350
CviJI RGCY 9 cut(s) 55, 63, 77, 89, 190, 205, 269, 298, 419
CviKI_1 RGCY 9 cut(s) 55, 63, 77, 89, 190, 205, 269, 298, 419
DdeI CTNAG 1 cut(s) 420
DpnI GATC 2 cut(s) 82, 110
DpnII GATC 2 cut(s) 80, 108
DraIII CACNNNGTG 1 cut(s) 10
DriI GACNNNNNGTC 1 cut(s) 288
Eam1105I GACNNNNNGTC 1 cut(s) 288
Eco147I AGGCCT 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 278
FaeI CATG 1 cut(s) 353
FaiI YATR 3 cut(s) 351, 432, 442
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FaqI GGGAC 1 cut(s) 37
FatI CATG 1 cut(s) 349
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 1 cut(s) 48
Fsp4HI GCNGC 1 cut(s) 117
FspBI CTAG 3 cut(s) 27, 299, 342
GlaI GCGC 1 cut(s) 32
GluI GCNGC 1 cut(s) 117
HaeIII GGCC 3 cut(s) 55, 89, 205
HapII CCGG 3 cut(s) 56, 66, 90
HhaI GCGC 1 cut(s) 33
Hin1II CATG 1 cut(s) 353
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 4 cut(s) 289, 365, 385, 392
HpaII CCGG 3 cut(s) 56, 66, 90
HphI GGTGA 1 cut(s) 80
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 3 cut(s) 278, 332, 389
Hpy8I GTNNAC 1 cut(s) 139
HpyCH4III ACNGT 1 cut(s) 8
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 6 cut(s) 4, 97, 119, 253, 338, 440
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 1 cut(s) 353
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 2 cut(s) 80, 108
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 9 cut(s) 63, 69, 79, 83, 103, 132, 255, 345, 402
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 1 cut(s) 325
MaeI CTAG 3 cut(s) 27, 299, 342
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 2 cut(s) 305, 344
MalI GATC 2 cut(s) 82, 110
MboI GATC 2 cut(s) 80, 108
MboII GAAGA 3 cut(s) 167, 238, 389
MluCI AATT 3 cut(s) 142, 222, 354
MlyI GAGTC 2 cut(s) 298, 374
MnlI CCTC 6 cut(s) 12, 33, 53, 64, 67, 258
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 2 cut(s) 317, 451
MspI CCGG 3 cut(s) 56, 66, 90
MspR9I CCNGG 1 cut(s) 57
Mva1269I GAATGC 1 cut(s) 99
MvnI CGCG 1 cut(s) 33
NciI CCSGG 1 cut(s) 57
NdeII GATC 2 cut(s) 80, 108
NlaIII CATG 1 cut(s) 353
NlaIV GGNNCC 2 cut(s) 64, 330
NmuCI GTSAC 2 cut(s) 305, 344
PceI AGGCCT 1 cut(s) 205
PctI GAATGC 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 2 cut(s) 385, 392
PkrI GCNGC 1 cut(s) 118
PleI GAGTC 2 cut(s) 297, 373
PpsI GAGTC 2 cut(s) 297, 373
PspN4I GGNNCC 2 cut(s) 64, 330
SaqAI TTAA 2 cut(s) 317, 451
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 2 cut(s) 80, 108
SchI GAGTC 2 cut(s) 298, 374
ScrFI CCNGG 1 cut(s) 57
SetI ASST 4 cut(s) 192, 222, 300, 330
SfaNI GCATC 1 cut(s) 325
SpeI ACTAGT 1 cut(s) 341
Sse9I AATT 3 cut(s) 142, 222, 354
SseBI AGGCCT 1 cut(s) 205
SsiI CCGC 2 cut(s) 33, 101
SspMI CTAG 3 cut(s) 27, 299, 342
StuI AGGCCT 1 cut(s) 205
StyD4I CCNGG 1 cut(s) 55
TaaI ACNGT 1 cut(s) 8
TaiI ACGT 1 cut(s) 222
TasI AATT 3 cut(s) 142, 222, 354
TfiI GAWTC 2 cut(s) 385, 392
Tru1I TTAA 2 cut(s) 317, 451
Tru9I TTAA 2 cut(s) 317, 451
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 2 cut(s) 305, 344
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 2 cut(s) 305, 344
TspDTI ATGAA 1 cut(s) 366
TspGWI ACGGA 2 cut(s) 270, 358
TspRI CASTG 1 cut(s) 406
XapI RAATTY 1 cut(s) 354
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 3 cut(s) 27, 299, 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.