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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 40 |
| AccII | CGCG | 4 cut(s) | 97, 151, 187, 189 |
| AciI | CCGC | 5 cut(s) | 45, 48, 92, 97, 151 |
| AclWI | GGATC | 2 cut(s) | 123, 131 |
| AlwI | GGATC | 2 cut(s) | 123, 131 |
| AoxI | GGCC | 1 cut(s) | 196 |
| ApeKI | GCWGC | 5 cut(s) | 58, 71, 108, 141, 159 |
| AspLEI | GCGC | 5 cut(s) | 41, 97, 151, 189, 191 |
| BbvI | GCAGC | 5 cut(s) | 45, 83, 95, 128, 171 |
| BccI | CCATC | 3 cut(s) | 7, 21, 130 |
| BfmI | CTRYAG | 3 cut(s) | 69, 109, 157 |
| BisI | GCNGC | 7 cut(s) | 46, 59, 72, 93, 109, 142, 160 |
| BlsI | GCNGC | 7 cut(s) | 47, 60, 73, 94, 110, 143, 161 |
| Bse118I | RCCGGY | 1 cut(s) | 198 |
| BseGI | GGATG | 3 cut(s) | 18, 32, 141 |
| BsePI | GCGCGC | 1 cut(s) | 187 |
| BseXI | GCAGC | 5 cut(s) | 45, 83, 95, 128, 171 |
| BseYI | CCCAGC | 3 cut(s) | 8, 131, 144 |
| Bsh1236I | CGCG | 4 cut(s) | 97, 151, 187, 189 |
| BshFI | GGCC | 1 cut(s) | 198 |
| BsiSI | CCGG | 3 cut(s) | 62, 121, 199 |
| BslFI | GGGAC | 1 cut(s) | 196 |
| BsmFI | GGGAC | 1 cut(s) | 196 |
| BsnI | GGCC | 1 cut(s) | 198 |
| Bsp143I | GATC | 3 cut(s) | 75, 115, 123 |
| BspACI | CCGC | 5 cut(s) | 45, 48, 92, 97, 151 |
| BspANI | GGCC | 1 cut(s) | 198 |
| BspFNI | CGCG | 4 cut(s) | 97, 151, 187, 189 |
| BspMAI | CTGCAG | 3 cut(s) | 73, 113, 161 |
| BspPI | GGATC | 2 cut(s) | 123, 131 |
| BsrFI | RCCGGY | 1 cut(s) | 198 |
| BssAI | RCCGGY | 1 cut(s) | 198 |
| BssHII | GCGCGC | 1 cut(s) | 187 |
| BssMI | GATC | 3 cut(s) | 75, 115, 123 |
| BstC8I | GCNNGC | 4 cut(s) | 20, 43, 90, 189 |
| BstF5I | GGATG | 3 cut(s) | 18, 32, 141 |
| BstFNI | CGCG | 4 cut(s) | 97, 151, 187, 189 |
| BstHHI | GCGC | 5 cut(s) | 41, 97, 151, 189, 191 |
| BstKTI | GATC | 3 cut(s) | 78, 118, 126 |
| BstMBI | GATC | 3 cut(s) | 75, 115, 123 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 28, 45, 94, 117, 168, 195 |
| BstSFI | CTRYAG | 3 cut(s) | 69, 109, 157 |
| BstUI | CGCG | 4 cut(s) | 97, 151, 187, 189 |
| BstV1I | GCAGC | 5 cut(s) | 45, 83, 95, 128, 171 |
| BsuRI | GGCC | 1 cut(s) | 198 |
| BtsCI | GGATG | 3 cut(s) | 18, 32, 141 |
| Cac8I | GCNNGC | 4 cut(s) | 20, 43, 90, 189 |
| CfoI | GCGC | 5 cut(s) | 41, 97, 151, 189, 191 |
| Cfr10I | RCCGGY | 1 cut(s) | 198 |
| CseI | GACGC | 1 cut(s) | 193 |
| CviAII | CATG | 1 cut(s) | 172 |
| CviJI | RGCY | 5 cut(s) | 31, 58, 141, 177, 198 |
| CviKI_1 | RGCY | 5 cut(s) | 31, 58, 141, 177, 198 |
| DpnI | GATC | 3 cut(s) | 77, 117, 125 |
| DpnII | GATC | 3 cut(s) | 75, 115, 123 |
| FaeI | CATG | 1 cut(s) | 175 |
| FaiI | YATR | 1 cut(s) | 173 |
| FaqI | GGGAC | 1 cut(s) | 196 |
| FatI | CATG | 1 cut(s) | 171 |
| FauI | CCCGC | 3 cut(s) | 41, 90, 144 |
| Fnu4HI | GCNGC | 7 cut(s) | 46, 59, 72, 93, 109, 142, 160 |
| FokI | GGATG | 3 cut(s) | 25, 39, 148 |
| Fsp4HI | GCNGC | 7 cut(s) | 46, 59, 72, 93, 109, 142, 160 |
| FspI | TGCGCA | 1 cut(s) | 40 |
| GlaI | GCGC | 5 cut(s) | 40, 96, 150, 188, 190 |
| GluI | GCNGC | 7 cut(s) | 46, 59, 72, 93, 109, 142, 160 |
| GsaI | CCCAGC | 3 cut(s) | 12, 135, 148 |
| HaeIII | GGCC | 1 cut(s) | 198 |
| HapII | CCGG | 3 cut(s) | 62, 121, 199 |
| HgaI | GACGC | 1 cut(s) | 193 |
| HhaI | GCGC | 5 cut(s) | 41, 97, 151, 189, 191 |
| Hin1II | CATG | 1 cut(s) | 175 |
| Hin6I | GCGC | 5 cut(s) | 39, 95, 149, 187, 189 |
| HinP1I | GCGC | 5 cut(s) | 39, 95, 149, 187, 189 |
| HinfI | GANTC | 1 cut(s) | 65 |
| HpaII | CCGG | 3 cut(s) | 62, 121, 199 |
| HpyCH4V | TGCA | 4 cut(s) | 71, 111, 159, 171 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 28, 45, 94, 117, 168, 195 |
| Hsp92II | CATG | 1 cut(s) | 175 |
| HspAI | GCGC | 5 cut(s) | 39, 95, 149, 187, 189 |
| Kzo9I | GATC | 3 cut(s) | 75, 115, 123 |
| Lsp1109I | GCAGC | 5 cut(s) | 45, 83, 95, 128, 171 |
| MalI | GATC | 3 cut(s) | 77, 117, 125 |
| MboI | GATC | 3 cut(s) | 75, 115, 123 |
| MnlI | CCTC | 2 cut(s) | 157, 188 |
| MspI | CCGG | 3 cut(s) | 62, 121, 199 |
| MvnI | CGCG | 4 cut(s) | 97, 151, 187, 189 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 28, 45, 94, 117, 168, 195 |
| NdeII | GATC | 3 cut(s) | 75, 115, 123 |
| NlaIII | CATG | 1 cut(s) | 175 |
| NsbI | TGCGCA | 1 cut(s) | 40 |
| PauI | GCGCGC | 1 cut(s) | 187 |
| PfeI | GAWTC | 1 cut(s) | 65 |
| PkrI | GCNGC | 7 cut(s) | 47, 60, 73, 94, 110, 143, 161 |
| PspFI | CCCAGC | 3 cut(s) | 8, 131, 144 |
| PstI | CTGCAG | 3 cut(s) | 73, 113, 161 |
| PteI | GCGCGC | 1 cut(s) | 187 |
| SatI | GCNGC | 7 cut(s) | 46, 59, 72, 93, 109, 142, 160 |
| Sau3AI | GATC | 3 cut(s) | 75, 115, 123 |
| SetI | ASST | 1 cut(s) | 168 |
| SfcI | CTRYAG | 3 cut(s) | 69, 109, 157 |
| SsiI | CCGC | 5 cut(s) | 45, 48, 92, 97, 151 |
| TauI | GCSGC | 2 cut(s) | 48, 95 |
| TfiI | GAWTC | 1 cut(s) | 65 |
| TseI | GCWGC | 5 cut(s) | 58, 71, 108, 141, 159 |