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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 4 cut(s) | 66, 92, 180, 209 |
| AciI | CCGC | 6 cut(s) | 49, 137, 207, 271, 293, 315 |
| AclWI | GGATC | 2 cut(s) | 286, 308 |
| AcoI | YGGCCR | 5 cut(s) | 3, 72, 160, 186, 204 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 12 |
| AluBI | AGCT | 2 cut(s) | 11, 20 |
| AluI | AGCT | 2 cut(s) | 11, 20 |
| Alw26I | GTCTC | 1 cut(s) | 238 |
| AlwI | GGATC | 2 cut(s) | 286, 308 |
| AoxI | GGCC | 6 cut(s) | 3, 72, 160, 186, 204, 257 |
| ApeKI | GCWGC | 8 cut(s) | 61, 87, 106, 116, 149, 175, 286, 308 |
| AspLEI | GCGC | 8 cut(s) | 54, 66, 80, 92, 142, 168, 180, 194 |
| AsuHPI | GGTGA | 1 cut(s) | 250 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BauI | CACGAG | 1 cut(s) | 153 |
| BbvI | GCAGC | 8 cut(s) | 48, 74, 118, 128, 136, 162, 273, 295 |
| BccI | CCATC | 1 cut(s) | 219 |
| BcoDI | GTCTC | 1 cut(s) | 238 |
| BfmI | CTRYAG | 3 cut(s) | 114, 287, 309 |
| BfoI | RGCGCY | 5 cut(s) | 55, 81, 143, 169, 195 |
| BmiI | GGNNCC | 1 cut(s) | 234 |
| BsaWI | WCCGGW | 1 cut(s) | 235 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 12 |
| Bse118I | RCCGGY | 3 cut(s) | 74, 162, 188 |
| Bse1I | ACTGG | 1 cut(s) | 252 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 12 |
| BseMII | CTCAG | 1 cut(s) | 27 |
| BseNI | ACTGG | 1 cut(s) | 252 |
| BseRI | GAGGAG | 1 cut(s) | 256 |
| BseXI | GCAGC | 8 cut(s) | 48, 74, 118, 128, 136, 162, 273, 295 |
| BseYI | CCCAGC | 1 cut(s) | 11 |
| BsgI | GTGCAG | 1 cut(s) | 135 |
| Bsh1236I | CGCG | 4 cut(s) | 66, 92, 180, 209 |
| BshFI | GGCC | 6 cut(s) | 5, 74, 162, 188, 206, 259 |
| BsiSI | CCGG | 5 cut(s) | 75, 163, 189, 236, 260 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 12 |
| BsmAI | GTCTC | 1 cut(s) | 238 |
| BsmI | GAATGC | 1 cut(s) | 269 |
| BsnI | GGCC | 6 cut(s) | 5, 74, 162, 188, 206, 259 |
| Bsp143I | GATC | 5 cut(s) | 93, 110, 120, 278, 300 |
| BspACI | CCGC | 6 cut(s) | 49, 137, 207, 271, 293, 315 |
| BspANI | GGCC | 6 cut(s) | 5, 74, 162, 188, 206, 259 |
| BspCNI | CTCAG | 1 cut(s) | 28 |
| BspFNI | CGCG | 4 cut(s) | 66, 92, 180, 209 |
| BspLI | GGNNCC | 1 cut(s) | 234 |
| BspMAI | CTGCAG | 3 cut(s) | 118, 291, 313 |
| BspPI | GGATC | 2 cut(s) | 286, 308 |
| BsrFI | RCCGGY | 3 cut(s) | 74, 162, 188 |
| BsrI | ACTGG | 1 cut(s) | 252 |
| BssAI | RCCGGY | 3 cut(s) | 74, 162, 188 |
| BssMI | GATC | 5 cut(s) | 93, 110, 120, 278, 300 |
| BssSI | CACGAG | 1 cut(s) | 153 |
| Bst2BI | CACGAG | 1 cut(s) | 153 |
| BstC8I | GCNNGC | 8 cut(s) | 76, 135, 164, 190, 204, 269, 291, 313 |
| BstDEI | CTNAG | 1 cut(s) | 36 |
| BstFNI | CGCG | 4 cut(s) | 66, 92, 180, 209 |
| BstH2I | RGCGCY | 5 cut(s) | 55, 81, 143, 169, 195 |
| BstHHI | GCGC | 8 cut(s) | 54, 66, 80, 92, 142, 168, 180, 194 |
| BstKTI | GATC | 5 cut(s) | 96, 113, 123, 281, 303 |
| BstMAI | GTCTC | 1 cut(s) | 238 |
| BstMBI | GATC | 5 cut(s) | 93, 110, 120, 278, 300 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 17, 58, 84, 139, 146, 172 |
| BstSFI | CTRYAG | 3 cut(s) | 114, 287, 309 |
| BstUI | CGCG | 4 cut(s) | 66, 92, 180, 209 |
| BstV1I | GCAGC | 8 cut(s) | 48, 74, 118, 128, 136, 162, 273, 295 |
| BsuRI | GGCC | 6 cut(s) | 5, 74, 162, 188, 206, 259 |
| Cac8I | GCNNGC | 8 cut(s) | 76, 135, 164, 190, 204, 269, 291, 313 |
| CfoI | GCGC | 8 cut(s) | 54, 66, 80, 92, 142, 168, 180, 194 |
| Cfr10I | RCCGGY | 3 cut(s) | 74, 162, 188 |
| DdeI | CTNAG | 1 cut(s) | 36 |
| DpnI | GATC | 5 cut(s) | 95, 112, 122, 280, 302 |
| DpnII | GATC | 5 cut(s) | 93, 110, 120, 278, 300 |
| EaeI | YGGCCR | 5 cut(s) | 3, 72, 160, 186, 204 |
| GlaI | GCGC | 8 cut(s) | 53, 65, 79, 91, 141, 167, 179, 193 |
| GsaI | CCCAGC | 1 cut(s) | 15 |
| HaeII | RGCGCY | 5 cut(s) | 55, 81, 143, 169, 195 |
| HaeIII | GGCC | 6 cut(s) | 5, 74, 162, 188, 206, 259 |
| HapII | CCGG | 5 cut(s) | 75, 163, 189, 236, 260 |
| HhaI | GCGC | 8 cut(s) | 54, 66, 80, 92, 142, 168, 180, 194 |
| Hin6I | GCGC | 8 cut(s) | 52, 64, 78, 90, 140, 166, 178, 192 |
| HinP1I | GCGC | 8 cut(s) | 52, 64, 78, 90, 140, 166, 178, 192 |
| HpaII | CCGG | 5 cut(s) | 75, 163, 189, 236, 260 |
| HphI | GGTGA | 1 cut(s) | 250 |
| Hpy188I | TCNGA | 1 cut(s) | 37 |
| HpyAV | CCTTC | 1 cut(s) | 249 |
| HpyCH4V | TGCA | 5 cut(s) | 116, 152, 267, 289, 311 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 17, 58, 84, 139, 146, 172 |
| HpyF3I | CTNAG | 1 cut(s) | 36 |
| HspAI | GCGC | 8 cut(s) | 52, 64, 78, 90, 140, 166, 178, 192 |
| KroI | GCCGGC | 3 cut(s) | 74, 162, 188 |
| KroNI | GCCGGC | 3 cut(s) | 76, 164, 190 |
| Kzo9I | GATC | 5 cut(s) | 93, 110, 120, 278, 300 |
| LmnI | GCTCC | 1 cut(s) | 238 |
| Lsp1109I | GCAGC | 8 cut(s) | 48, 74, 118, 128, 136, 162, 273, 295 |
| MalI | GATC | 5 cut(s) | 95, 112, 122, 280, 302 |
| MboI | GATC | 5 cut(s) | 93, 110, 120, 278, 300 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MnlI | CCTC | 5 cut(s) | 31, 39, 212, 223, 234 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| MroNI | GCCGGC | 3 cut(s) | 74, 162, 188 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspI | CCGG | 5 cut(s) | 75, 163, 189, 236, 260 |
| Mva1269I | GAATGC | 1 cut(s) | 269 |
| MvnI | CGCG | 4 cut(s) | 66, 92, 180, 209 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 17, 58, 84, 139, 146, 172 |
| NaeI | GCCGGC | 3 cut(s) | 76, 164, 190 |
| NdeII | GATC | 5 cut(s) | 93, 110, 120, 278, 300 |
| NgoMIV | GCCGGC | 3 cut(s) | 74, 162, 188 |
| NlaIV | GGNNCC | 1 cut(s) | 234 |
| PctI | GAATGC | 1 cut(s) | 269 |
| PdiI | GCCGGC | 3 cut(s) | 76, 164, 190 |
| PspFI | CCCAGC | 1 cut(s) | 11 |
| PspN4I | GGNNCC | 1 cut(s) | 234 |
| PstI | CTGCAG | 3 cut(s) | 118, 291, 313 |
| Sau3AI | GATC | 5 cut(s) | 93, 110, 120, 278, 300 |
| SetI | ASST | 2 cut(s) | 13, 22 |
| SfcI | CTRYAG | 3 cut(s) | 114, 287, 309 |
| SsiI | CCGC | 6 cut(s) | 49, 137, 207, 271, 293, 315 |
| TauI | GCSGC | 3 cut(s) | 52, 140, 209 |
| TseI | GCWGC | 8 cut(s) | 61, 87, 106, 116, 149, 175, 286, 308 |