Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001111

Physical Location & Seq

Genomic Coordinates Reverse (-)

31134341 .. 31142980

Gene Structure 8640 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1182800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4089 bp

ATGGAGGAAGCGCTGGAGCTCGCGCGTGCCAAGGACACCAAGGAGCGCATGGCGGGAGTGGAGCGCCTGCATCAGCTTCTGGAAGCGTCCAGAAAGAGCCTGAGTTCGTCGGAGGTGACGTCGCTCGTCGACTGCTGCTTGGATCTTCTCAAGGACAACAACTTCAGAGTCTCCCAGGGCGCTCTGCAGGCACTGGCCTCCGCCGCCGTGCTCTCCGGCGACCACCTCAAGCTCCATTTCAATGCGCTTGTTCCCGCCGTCGTCGAGCGTTTGGGCGATGGCAAACAGCCCGTTCGCGATGCCGCTCGGAGGCTCCTGCTTACTCTCATGGAGGTTTCTTCTCCGACAATCATTGTTGAAAGAGCAGGATCTTATGCCTGGGCGCACAAAAGCTGGAGAGTTCGAGAAGAGTTTGCGCGGACGGTCACCTCAGCAATTGGGCTTTTTGCATCAACTGAACTTCCACTTCAGCGTACTATACTTCCTCCGATTCTACAGATGTTAAGTGATCCAAATCCTGGTGTAAGGGATGCAGCTATAGCATGCATTGAGGAGATGTATACGCAGGCAGGGCCTCAATTCCGTGATGAACTTCAGAGGCACCACCTTCCTATGTCTATGCTGAAGGATATTAATGCGAGGTTAGAGAGGATTGAACCTAAGATTCGCTCGTCAGATGGACTTAGTGCTGTAGAAGCGAAGCCTGTGAACCTTAACCACAAGAAAAGTAGTCCAAAGGCGAAAAGTTCCTCAAGGGAGGCGTCTCTTTTTGGAGCCGAAACTGATGCTGCTGAAAAAGCCGCAGACCCTATTAAAGTTTATTCAGAAAAGGAACTAATAAGGGAAATTGAGAAAATTGCTTCCACCCTTGTACCAGAGAAAGACTGGTCAATTCGCATTGCTGCAATGCAGAGAATTGAAGGACTTGTATATGGAGGTGCTGCTGACTACCAATGTTTCCGGGGACTCCTGAAGCAACTTGTTGGGCCTCTGAGCACACAATTATCTGATCGAAGGTCCAGCATAGTGAAACAGGCTTGCCATCTGTTATGCTTTTTGTCAAAGGAGTTACTAGGAGATTTTGAGACATGTGCGGAGGTGTTCATTCCAGTTCTTTTCAAGCTGGTTGTGATTACTGTACTTGTAATTGCAGAGTCTGCAGATAACTGCATAAAAACGATGTTGCGTAATTGTAAAGTTGCTCGCGTGCTCCCTCGTATTGCTGATTGTGCAAAGAATGACCGTAATGCAATACTTCGTGCAAGGTGCTGTGATTATGCACTGTTGATACTAGAGTATTGGGCTGATGCACCTGAAATACAGCGATCAGCGGATTTGTATGAGGATCTAATAAGGTGTTGTGTGGCAGATGCAATGAGTGAGGTCCGATCAACTGCAAGAATGTGCTATAGAATGTTCTCGAAAACATGGCCAGAGCGTTCCCGACGGTTATTTTCTCTATTTGATCCTGTTATTCAAAGGTTAATCAATGAAGAGGATGGGGGAGTACATAGGCGTCATGCTTCTCCTTCTGTTCGTGATAGGGGTGTGTCACATACTCCCCAGCTTTCTGCTACTTCAAATTTGCCTGGGTATGGAACTTCAGCTATTGTTGCAATGGATAGAAGTTCAAGTTTATCAACTGGCAATTCTATCTCTTCGGGACTACTTCTGTCGCAAGCAAAGTCACTTGGTAAAGGTACTGAACGTAGTCTGGAAAGTGTGTTGCATGCAAGCAAACAGAAGGTCAGTGCAATTGAAAGCATGCTTAGAGGTTTGGATTTGTCTGAGAAACATAATTCAACTCTCCGGTCATCAAGCCTGGATCTAGGAGTTGACCCTCCATCATCTCGTGATCCACCATTCCCTGCTGCTGCCCCAGCTTCTAATCATCTCTCAAACTCTTTAATGGCAGATTCAACTACCTCAAGTATCCATAAAAGTAGTAGCCGCAATGGTGGCTTGGTTTTGTCTGATATCATTACACAAATTCAAGCTTCCAAAGATTCGGGTAAATCGTCATATCGCAGTAATCAATCGGCAGAAGCTATGCCAACTGTTTCGTCATACGCAATGAGGAGACCTTCCGAGAGAACCCATGAAAGGGGTTCCATTGAAGAGAACAATGACACTAGGGAGGCTAGACGATTCATGAACTCTCAAATCGATAGGCACTATGACACGTCTCACAGAGATGGGAACTTTAGGGATTCAAATAGCAATCACGTGCCCAATTTCCAAAGGCCACTCTTAAGGAAGAATGTGACTGGAAGGATGTCCGCAGGCAGGAGGAGAAGTTTTGATGATAGTCAACTCTCACTTGGGGAAATGTCCAATTATGTAGAAGGTCCCACATCTCTAAATGATGCTTTGAGTGAGGGACTGAATCCATCGTCTGACTGGAATGCTAGGGTTGCTGCATTTAATTATCTACGATCTTTGCTGCAGCAAGGCCCAAGGGGAATTCAAGAAGTGATTCAGAATTTTGAGAAAGTAATGAAGTTGTTTTTCCAACACTTGGATGATCCCCACCATAAAGTTGCTCAGGCAGCTCTTTCAACACTAGCAGATATTATTCCATCTTGCCGTAAGCCCTTTGAAAGTTACATGGAAAGGATCCTACCCCACGTTTTCTCACGGTTAATTGACCCAAAGGAGTTAGTTAGGCAGCCGTGCTCGACAACGTTGGATATTGTTAGCAAAACTTACAGCGTAGATTCCCTCTTGCCTGCTTTGCTCCGTTCACTAGATGAGCAGCGATCACCAAAGGCCAAACTAGCTGTTATTGAGTTTTCCATTAGTTCTTTTAACAAGCATTCACTGAATCCTGAAGGTTCTGGTAATACTGGCATCCTTAAATTATGGCTTTCTAAGTTGGCGCCATTGGTCCATGATAAAAATACTAAACTAAAGGAGGCAGCCATTACGTGCATAATATCTGTTTACTCCCACTTTGATTCTGTAGCTGTCTTGAATTTTATTCTTAGTTTGTCAGTTGAAGAACAAAATTCTTTGAGAAGGGCCCTGAAACAGTACACTCCTCGTATTGAAGTGGACCTTATGAACTTTTTGCAAAACAAGAAAGAGCGACAGCGCCCTAAATCTTCTTATGATCCATCTGATGTAGTTGGAACATCTTCTGAAGAAGGATATGTTAGTGCCTCAAAGAAGAGTCACTTTGTTGGGAGGTATTCTGCTGGTTCAGTTGATATTGATGGTGGCAGGAAGTGGAGTTCAACCCAAGAATCGGCCATGGTCACCAGTACTATTGGTCAAGCAGCTTCTGATGAAACACGGGAGAACTTGTATCAGAATTTTGAGACTGGTTCCAACACTGATATTCTCAATTCAAAATTCAAGGATATGTCTTACACTATGAATTCTGTGAGTCAGAACTTGGGCTCCTGGAGTAGCCCAGTGGATAAAGTTGATGGTAGAACAAATTTAGAAGGCTTGTCTACTACTTGCCTAGATGTTAATGGTCTTATGAGCTTGGACCATATAGGGGTTGCTGAGAGTACTGGGCATGATAGTGAAGCTTCCACCGACTTGGACTCTAATCATTATAATCTTACAGCTTTGAAGGTTAATTCTACACCAGAATCAGGTCCCAGCATTCCTCAAATACTTCATCTGATCGGCAATGGAACTGAGGAAAGCCCTACAGCAAGCAAACGTGGTGCACTTCAGCAATTAATTGATGCTTCCATAGCTAATGACCACTCTGTTTGGACCAAGTACTTCAATCAGATTTTGACTGTTGTACTTGAGGTGTTGGATGATTTGAATTCCTCGATTCGGGAACTCTCTCTTTCCTTGATTGTCGAAATGCTTAAAAACCAGAAAGATGCAATGGAAGATTCTGTTGAGATCGTAATTGAGAAACTGCTTCATGTTACGAAGGATGTAGTTCCCAAGGTTTCAAATGAATCTGAGCACTGCTTGAGCATCGTTTTGGCCCAGTATGATCCATTCAGATGCCTAAGTGTTATTGTCCCTCTGTTGGTAACTGAAGATGAGAAAATTCTTGTTACTTGCATCAACTGTTTGACAAAGGTATTATGCTTCCATGTATTGGAGCTGTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000902 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0005876 GO:0005886 GO:0005911 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007026 GO:0007049 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009653 GO:0009826 GO:0009987 GO:0010639 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0022607 GO:0030054 GO:0030865 GO:0031023 GO:0031109 GO:0031110 GO:0031111 GO:0031114 GO:0031122 GO:0031647 GO:0032502 GO:0032886 GO:0032989 GO:0033043 GO:0034453 GO:0034622 GO:0040007 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043622 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0046785 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050821 GO:0051010 GO:0051128 GO:0051129 GO:0051258 GO:0051302 GO:0051493 GO:0051494 GO:0051781 GO:0055044 GO:0060560 GO:0065003 GO:0065007 GO:0065008 GO:0070507 GO:0071840 GO:0071944 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1901879 GO:1901880 GO:1902903 GO:1902904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CLASP_N HEAT Vac14_Fab1_bd

Protein Analysis

1363

Amino Acids

150.69

Weight (kDa)

6.61

Isoelectric Point (pI)

47.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CEP104-like_TOG	PF21040	10 - 180	9e-10	Centrosomal protein CEP104-like, TOG domain
XMAP215_CLASP_TOG	PF21041	11 - 213	1.2e-09	XMAP215/Dis1/CLASP, TOG domain
HEAT_GCN1_C	PF25786	18 - 116	3.9e-07	GCN1-like, C-terminal HEAT repeats
HEAT	PF02985	160 - 187	7.2e-06	HEAT repeat
CLASP_N	PF12348	280 - 496	5.1e-48	CLASP N terminal
CLASP_N	PF12348	793 - 987	9.3e-10	CLASP N terminal
CEP104-like_TOG	PF21040	798 - 942	2.7e-08	Centrosomal protein CEP104-like, TOG domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001111)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20190
fragaria_vesca	FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_4g18130
malus_domestica	MD05G1182800.v1.1 MD10G1170900.v1.1
prunus_persica	Prupe.8G209400_v2.0.a1 Prupe.8G209400_v2.0.a1 Prupe.8G209400_v2.0.a1
pyrus_communis	pycom05g16790 pycom10g14730
rosa_chinensis	RchiOBHm_Chr1g0330771 RchiOBHm_Chr1g0330781 RchiOBHm_Chr1g0330801 RchiOBHm_Chr6g0285551
rosa_laevigata	RLG00000012628 RLG00000029789 RLG00000029790 RLG00000029796
rosa_multiflora	Rmu_co8432325.1_g000001 Rmu_sc0000723.1_g000001 Rmu_sc0000723.1_g000002 Rmu_sc0001491.1_g000006 Rmu_sc0002725.1_g000020 Rmu_sc0012495.1_g000004 Rmu_sc0017770.1_g000001 Rmu_sc0031058.1_g000001
rosa_roxburghii	Rroxscaffold_4G00319710 Rroxscaffold_4G00319750 Rroxscaffold_4G00319790 Rroxscaffold_4G00319800 Rroxscaffold_4G00319810 Rroxscaffold_7G00183030
rosa_rugosa	Rorug06G0175400
rosa_samantha	Rh1AG108200 Rh1AG109200 Rh1AG110500 Rh1AG111400 Rh1CG105100 Rh1CG105400 Rh1CG105500 Rh1CG106800 Rh2BG359900 Rh2DG376700 Rh4CG127100 Rh6AG287000 Rh6BG288200 Rh6CG289400 Rh6DG282200
rosa_wichuraiana	Rw0G004360 Rw1G008500 Rw1G008580 Rw1G008750 Rw1G008840 Rw6G024670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3567
AatII	GACGTC	1 cut(s)	122
AccB1I	GGYRCC	2 cut(s)	600, 2878
AccB7I	CCANNNNNTGG	3 cut(s)	518, 2518, 4075
AccBSI	CCGCTC	1 cut(s)	305
AccI	GTMKAC	3 cut(s)	129, 560, 3458
AccII	CGCG	5 cut(s)	23, 25, 297, 418, 1206
AclI	AACGTT	1 cut(s)	2684
AcoI	YGGCCR	2 cut(s)	1430, 3249
AcvI	CACGTG	1 cut(s)	2227
AcyI	GRCGYC	4 cut(s)	119, 761, 1516, 2879
AfeI	AGCGCT	1 cut(s)	12
AflII	CTTAAG	1 cut(s)	2251
AflIII	ACRYGT	2 cut(s)	1088, 2181
AjiI	CACGTC	1 cut(s)	2184
AjnI	CCWGG	6 cut(s)	174, 377, 517, 1588, 1821, 3404
AloI	GAACNNNNNNTCC	4 cut(s)	3217, 3249, 3386, 3418
Alw21I	GWGCWC	7 cut(s)	21, 213, 998, 1212, 2678, 3685, 3939
Alw26I	GTCTC	6 cut(s)	175, 768, 1079, 2074, 2190, 3314
Alw44I	GTGCAC	1 cut(s)	3681
AlwNI	CAGNNNCTG	4 cut(s)	79, 193, 1157, 3284
Aor51HI	AGCGCT	1 cut(s)	12
ApaI	GGGCCC	1 cut(s)	3025
ApaLI	GTGCAC	1 cut(s)	3681
ArsI	GACNNNNNNTTYG	2 cut(s)	1731, 1763
AseI	ATTAAT	2 cut(s)	633, 3695
Asp700I	GAANNNNTTC	2 cut(s)	2475, 3136
AsuC2I	CCSGG	1 cut(s)	962
AsuHPI	GGTGA	4 cut(s)	127, 418, 2754, 3250
AvaII	GGWCC	8 cut(s)	1017, 1384, 2348, 2887, 3055, 3496, 3608, 3732
BaeGI	GKGCMC	3 cut(s)	2232, 3025, 3685
BaeI	ACNNNNGTAYC	4 cut(s)	855, 888, 1280, 1313
BalI	TGGCCA	1 cut(s)	1432
BamHI	GGATCC	1 cut(s)	2616
BanI	GGYRCC	2 cut(s)	600, 2878
BanII	GRGCYC	3 cut(s)	21, 3025, 3404
BauI	CACGAG	1 cut(s)	1851
BbrPI	CACGTG	1 cut(s)	2227
Bbv12I	GWGCWC	7 cut(s)	21, 213, 998, 1212, 2678, 3685, 3939
BbvCI	CCTCAGC	1 cut(s)	430
BceAI	ACGGC	4 cut(s)	191, 242, 2571, 2656
BcgI	CGANNNNNNTGC	2 cut(s)	767, 801
BciT130I	CCWGG	6 cut(s)	176, 379, 519, 1590, 1823, 3406
BciVI	GTATCC	1 cut(s)	1943
BcnI	CCSGG	1 cut(s)	962
BcoDI	GTCTC	6 cut(s)	175, 768, 1079, 2074, 2190, 3314
BfmI	CTRYAG	9 cut(s)	185, 494, 537, 690, 1158, 1408, 2444, 2961, 3663
BfoI	RGCGCY	5 cut(s)	14, 67, 183, 2882, 3097
BfrI	CTTAAG	1 cut(s)	2251
BfuI	GTATCC	1 cut(s)	1943
BmcAI	AGTACT	3 cut(s)	3265, 3520, 3740
Bme1390I	CCNGG	7 cut(s)	176, 379, 519, 962, 1590, 1823, 3406
Bme18I	GGWCC	8 cut(s)	1017, 1384, 2348, 2887, 3055, 3496, 3608, 3732
BmgBI	CACGTC	1 cut(s)	2184
BmrFI	CCNGG	7 cut(s)	176, 379, 519, 962, 1590, 1823, 3406
BmrI	ACTGGG	3 cut(s)	3410, 3531, 3955
BmuI	ACTGGG	3 cut(s)	3410, 3531, 3955
BpmI	CTGGAG	3 cut(s)	35, 415, 3427
Bpu10I	CCTNAGC	2 cut(s)	430, 2544
BpuEI	CTTGAG	6 cut(s)	134, 212, 736, 1912, 3788, 3964
BpuMI	CCSGG	1 cut(s)	962
Bsa29I	ATCGAT	1 cut(s)	2166
BsaAI	YACGTR	2 cut(s)	2227, 2928
BsaBI	GATNNNNATC	1 cut(s)	513
BsaHI	GRCGYC	4 cut(s)	119, 761, 1516, 2879
BsaI	GGTCTC	1 cut(s)	2074
BsaWI	WCCGGW	1 cut(s)	1809
BsaXI	ACNNNNNCTCC	8 cut(s)	104, 134, 2281, 2311, 3290, 3320, 3386, 3416
Bse3DI	GCAATG	8 cut(s)	897, 912, 1380, 1623, 1960, 2079, 3649, 3858
Bse8I	GATNNNNATC	1 cut(s)	513
BseBI	CCWGG	6 cut(s)	176, 379, 519, 1590, 1823, 3406
BseCI	ATCGAT	1 cut(s)	2166
BseGI	GGATG	7 cut(s)	535, 1504, 2280, 2527, 2850, 3784, 3910
BseJI	GATNNNNATC	1 cut(s)	513
BseMI	GCAATG	8 cut(s)	897, 912, 1380, 1623, 1960, 2079, 3649, 3858
BseMII	CTCAG	8 cut(s)	92, 444, 983, 1779, 2558, 3504, 3642, 3924
BseRI	GAGGAG	4 cut(s)	566, 2092, 2305, 3030
BseSI	GKGCMC	3 cut(s)	2232, 3025, 3685
BseYI	CCCAGC	3 cut(s)	1563, 1879, 3611
Bsh1236I	CGCG	5 cut(s)	23, 25, 297, 418, 1206
BshNI	GGYRCC	2 cut(s)	600, 2878
BshVI	ATCGAT	1 cut(s)	2166
BsiHKAI	GWGCWC	7 cut(s)	21, 213, 998, 1212, 2678, 3685, 3939
BsiSI	CCGG	3 cut(s)	216, 961, 1810
BslFI	GGGAC	6 cut(s)	978, 1677, 2334, 2394, 3594, 3980
BsmAI	GTCTC	6 cut(s)	175, 768, 1079, 2074, 2190, 3314
BsmBI	CGTCTC	2 cut(s)	768, 2190
BsmFI	GGGAC	6 cut(s)	978, 1677, 2334, 2394, 3594, 3980
BsmI	GAATGC	3 cut(s)	2410, 2815, 3615
Bso31I	GGTCTC	1 cut(s)	2074
Bsp120I	GGGCCC	1 cut(s)	3021
Bsp19I	CCATGG	1 cut(s)	3252
Bsp68I	TCGCGA	1 cut(s)	297
BspCNI	CTCAG	8 cut(s)	93, 443, 984, 1780, 2557, 3505, 3643, 3925
BspDI	ATCGAT	1 cut(s)	2166
BspFNI	CGCG	5 cut(s)	23, 25, 297, 418, 1206
BspHI	TCATGA	1 cut(s)	2151
BspMAI	CTGCAG	3 cut(s)	189, 1162, 2448
BspT107I	GGYRCC	2 cut(s)	600, 2878
BspTI	CTTAAG	1 cut(s)	2251
BspTNI	GGTCTC	1 cut(s)	2074
BsrBI	CCGCTC	1 cut(s)	305
BsrDI	GCAATG	8 cut(s)	897, 912, 1380, 1623, 1960, 2079, 3649, 3858
BssNAI	GTATAC	1 cut(s)	561
BssNI	GRCGYC	4 cut(s)	119, 761, 1516, 2879
BssSI	CACGAG	1 cut(s)	1851
BssT1I	CCWWGG	5 cut(s)	30, 39, 2456, 3252, 3915
Bst1107I	GTATAC	1 cut(s)	561
Bst2BI	CACGAG	1 cut(s)	1851
Bst2UI	CCWGG	6 cut(s)	176, 379, 519, 1590, 1823, 3406
Bst6I	CTCTTC	5 cut(s)	402, 1488, 1663, 2112, 3164
BstACI	GRCGYC	4 cut(s)	119, 761, 1516, 2879
BstAFI	CTTAAG	1 cut(s)	2251
BstAPI	GCANNNNNTGC	1 cut(s)	1157
BstBAI	YACGTR	2 cut(s)	2227, 2928
BstDSI	CCRYGG	1 cut(s)	3252
BstEII	GGTNACC	2 cut(s)	424, 3256
BstF5I	GGATG	7 cut(s)	535, 1504, 2280, 2527, 2850, 3784, 3910
BstFNI	CGCG	5 cut(s)	23, 25, 297, 418, 1206
BstH2I	RGCGCY	5 cut(s)	14, 67, 183, 2882, 3097
BstMAI	GTCTC	6 cut(s)	175, 768, 1079, 2074, 2190, 3314
BstNI	CCWGG	6 cut(s)	176, 379, 519, 1590, 1823, 3406
BstNSI	RCATGY	4 cut(s)	546, 1092, 1733, 1768
BstPI	GGTNACC	2 cut(s)	424, 3256
BstSCI	CCNGG	7 cut(s)	174, 377, 517, 960, 1588, 1821, 3404
BstSFI	CTRYAG	9 cut(s)	185, 494, 537, 690, 1158, 1408, 2444, 2961, 3663
BstSLI	GKGCMC	3 cut(s)	2232, 3025, 3685
BstUI	CGCG	5 cut(s)	23, 25, 297, 418, 1206
BstX2I	RGATCY	5 cut(s)	142, 368, 1345, 1825, 2616
BstXI	CCANNNNNNTGG	1 cut(s)	3550
BstYI	RGATCY	5 cut(s)	142, 368, 1345, 1825, 2616
BstZ17I	GTATAC	1 cut(s)	561
Bsu15I	ATCGAT	1 cut(s)	2166
BsuI	GTATCC	1 cut(s)	1943
BsuTUI	ATCGAT	1 cut(s)	2166
BtgI	CCRYGG	1 cut(s)	3252
BtgZI	GCGATG	2 cut(s)	291, 312
BtrI	CACGTC	1 cut(s)	2184
BtsCI	GGATG	7 cut(s)	535, 1504, 2280, 2527, 2850, 3784, 3910
BtsI	GCAGTG	1 cut(s)	3937
BtsIMutI	CAGTG	7 cut(s)	191, 1280, 1756, 2819, 3333, 3423, 3937
BtuMI	TCGCGA	1 cut(s)	297
CaiI	CAGNNNCTG	4 cut(s)	79, 193, 1157, 3284
CciI	TCATGA	1 cut(s)	2151
ClaI	ATCGAT	1 cut(s)	2166
CseI	GACGC	3 cut(s)	75, 750, 1505
CspCI	CAANNNNNGTGG	2 cut(s)	3710, 3745
DinI	GGCGCC	1 cut(s)	2880
EaeI	YGGCCR	2 cut(s)	1430, 3249
Eam1104I	CTCTTC	5 cut(s)	402, 1488, 1663, 2112, 3164
EarI	CTCTTC	5 cut(s)	402, 1488, 1663, 2112, 3164
EciI	GGCGGA	1 cut(s)	190
Ecl136II	GAGCTC	1 cut(s)	19
Eco130I	CCWWGG	5 cut(s)	30, 39, 2456, 3252, 3915
Eco24I	GRGCYC	3 cut(s)	21, 3025, 3404
Eco31I	GGTCTC	1 cut(s)	2074
Eco32I	GATATC	1 cut(s)	1978
Eco47I	GGWCC	8 cut(s)	1017, 1384, 2348, 2887, 3055, 3496, 3608, 3732
Eco47III	AGCGCT	1 cut(s)	12
Eco53kI	GAGCTC	1 cut(s)	19
Eco72I	CACGTG	1 cut(s)	2227
Eco91I	GGTNACC	2 cut(s)	424, 3256
EcoICRI	GAGCTC	1 cut(s)	19
EcoO109I	RGGNCCY	5 cut(s)	572, 2348, 3021, 3022, 3608
EcoO65I	GGTNACC	2 cut(s)	424, 3256
EcoRI	GAATTC	3 cut(s)	2463, 3379, 3787
EcoRII	CCWGG	6 cut(s)	174, 377, 517, 1588, 1821, 3404
EcoRV	GATATC	1 cut(s)	1978
EcoT14I	CCWWGG	5 cut(s)	30, 39, 2456, 3252, 3915
EcoT22I	ATGCAT	1 cut(s)	548
EcoT38I	GRGCYC	3 cut(s)	21, 3025, 3404
EgeI	GGCGCC	1 cut(s)	2880
EheI	GGCGCC	1 cut(s)	2880
ErhI	CCWWGG	5 cut(s)	30, 39, 2456, 3252, 3915
Esp3I	CGTCTC	2 cut(s)	768, 2190
FalI	AAGNNNNNCTT	4 cut(s)	3042, 3074, 3161, 3193
FaqI	GGGAC	6 cut(s)	978, 1677, 2334, 2394, 3594, 3980
FauI	CCCGC	2 cut(s)	46, 262
FblI	GTMKAC	3 cut(s)	129, 560, 3458
FokI	GGATG	7 cut(s)	542, 1511, 2287, 2534, 2837, 3791, 3917
FriOI	GRGCYC	3 cut(s)	21, 3025, 3404
GsaI	CCCAGC	3 cut(s)	1567, 1883, 3615
GsuI	CTGGAG	3 cut(s)	35, 415, 3427
HaeII	RGCGCY	5 cut(s)	14, 67, 183, 2882, 3097
HapII	CCGG	3 cut(s)	216, 961, 1810
HgaI	GACGC	3 cut(s)	75, 750, 1505
Hin1I	GRCGYC	4 cut(s)	119, 761, 1516, 2879
HincII	GTYRAC	3 cut(s)	130, 1837, 2312
HindII	GTYRAC	3 cut(s)	130, 1837, 2312
HindIII	AAGCTT	2 cut(s)	1995, 3537
HpaII	CCGG	3 cut(s)	216, 961, 1810
HphI	GGTGA	4 cut(s)	127, 418, 2754, 3250
Hpy99I	CGWCG	6 cut(s)	112, 124, 131, 263, 266, 1449
HpyCH4IV	ACGT	8 cut(s)	119, 1708, 2183, 2226, 2628, 2684, 2927, 3676
HpySE526I	ACGT	8 cut(s)	119, 1708, 2183, 2226, 2628, 2684, 2927, 3676
Hsp92I	GRCGYC	4 cut(s)	119, 761, 1516, 2879
KasI	GGCGCC	1 cut(s)	2878
MaeII	ACGT	8 cut(s)	119, 1708, 2183, 2226, 2628, 2684, 2927, 3676
MbiI	CCGCTC	1 cut(s)	305
MfeI	CAATTG	2 cut(s)	435, 1755
MflI	RGATCY	5 cut(s)	142, 368, 1345, 1825, 2616
MlsI	TGGCCA	1 cut(s)	1432
MluNI	TGGCCA	1 cut(s)	1432
Mly113I	GGCGCC	1 cut(s)	2879
MlyI	GAGTC	6 cut(s)	177, 960, 1163, 3181, 3397, 3548
MmeI	TCCRAC	7 cut(s)	90, 368, 2536, 2667, 3109, 3354, 3756
Mox20I	TGGCCA	1 cut(s)	1432
Mph1103I	ATGCAT	1 cut(s)	548
MroXI	GAANNNNTTC	2 cut(s)	2475, 3136
MscI	TGGCCA	1 cut(s)	1432
MslI	CAYNNNNRTG	5 cut(s)	240, 2193, 2520, 3531, 3976
Msp20I	TGGCCA	1 cut(s)	1432
MspA1I	CMGCKG	1 cut(s)	1331
MspCI	CTTAAG	1 cut(s)	2251
MspI	CCGG	3 cut(s)	216, 961, 1810
MspR9I	CCNGG	7 cut(s)	176, 379, 519, 962, 1590, 1823, 3406
MunI	CAATTG	2 cut(s)	435, 1755
Mva1269I	GAATGC	3 cut(s)	2410, 2815, 3615
MvaI	CCWGG	6 cut(s)	176, 379, 519, 1590, 1823, 3406
MvnI	CGCG	5 cut(s)	23, 25, 297, 418, 1206
NarI	GGCGCC	1 cut(s)	2879
NciI	CCSGG	1 cut(s)	962
NcoI	CCATGG	1 cut(s)	3252
NmuCI	GTSAC	7 cut(s)	115, 424, 1551, 1686, 2263, 3173, 3256
NruI	TCGCGA	1 cut(s)	297
NsiI	ATGCAT	1 cut(s)	548
NspI	RCATGY	4 cut(s)	546, 1092, 1733, 1768
PaeI	GCATGC	3 cut(s)	546, 1733, 1768
PagI	TCATGA	1 cut(s)	2151
PasI	CCCWGGG	1 cut(s)	175
PciI	ACATGT	1 cut(s)	1088
PcsI	WCGNNNNNNNCGW	2 cut(s)	116, 2015
PctI	GAATGC	3 cut(s)	2410, 2815, 3615
PdmI	GAANNNNTTC	2 cut(s)	2475, 3136
PflMI	CCANNNNNTGG	3 cut(s)	518, 2518, 4075
PfoI	TCCNGGA	1 cut(s)	3404
PleI	GAGTC	6 cut(s)	176, 960, 1162, 3180, 3396, 3548
PluTI	GGCGCC	1 cut(s)	2882
PmaCI	CACGTG	1 cut(s)	2227
PmlI	CACGTG	1 cut(s)	2227
PpsI	GAGTC	6 cut(s)	176, 960, 1162, 3180, 3396, 3548
Ppu21I	YACGTR	2 cut(s)	2227, 2928
PpuMI	RGGWCCY	2 cut(s)	2348, 3608
PscI	ACATGT	1 cut(s)	1088
PshBI	ATTAAT	2 cut(s)	633, 3695
PsiI	TTATAA	1 cut(s)	3567
Psp124BI	GAGCTC	1 cut(s)	21
Psp1406I	AACGTT	1 cut(s)	2684
Psp5II	RGGWCCY	2 cut(s)	2348, 3608
Psp6I	CCWGG	6 cut(s)	174, 377, 517, 1588, 1821, 3404
PspCI	CACGTG	1 cut(s)	2227
PspEI	GGTNACC	2 cut(s)	424, 3256
PspFI	CCCAGC	3 cut(s)	1563, 1879, 3611
PspGI	CCWGG	6 cut(s)	174, 377, 517, 1588, 1821, 3404
PspOMI	GGGCCC	1 cut(s)	3021
PspPPI	RGGWCCY	2 cut(s)	2348, 3608
PstI	CTGCAG	3 cut(s)	189, 1162, 2448
PstNI	CAGNNNCTG	4 cut(s)	79, 193, 1157, 3284
PsuI	RGATCY	5 cut(s)	142, 368, 1345, 1825, 2616
RruI	TCGCGA	1 cut(s)	297
RseI	CAYNNNNRTG	5 cut(s)	240, 2193, 2520, 3531, 3976
SacI	GAGCTC	1 cut(s)	21
SalI	GTCGAC	1 cut(s)	128
ScaI	AGTACT	3 cut(s)	3265, 3520, 3740
SchI	GAGTC	6 cut(s)	177, 960, 1163, 3181, 3397, 3548
ScrFI	CCNGG	7 cut(s)	176, 379, 519, 962, 1590, 1823, 3406
SfcI	CTRYAG	9 cut(s)	185, 494, 537, 690, 1158, 1408, 2444, 2961, 3663
SfoI	GGCGCC	1 cut(s)	2880
SinI	GGWCC	8 cut(s)	1017, 1384, 2348, 2887, 3055, 3496, 3608, 3732
SmiMI	CAYNNNNRTG	5 cut(s)	240, 2193, 2520, 3531, 3976
SmlI	CTYRAG	7 cut(s)	149, 227, 751, 1927, 2251, 3767, 3943
SmoI	CTYRAG	7 cut(s)	149, 227, 751, 1927, 2251, 3767, 3943
SphI	GCATGC	3 cut(s)	546, 1733, 1768
SspDI	GGCGCC	1 cut(s)	2878
SstI	GAGCTC	1 cut(s)	21
StyD4I	CCNGG	7 cut(s)	174, 377, 517, 960, 1588, 1821, 3404
StyI	CCWWGG	5 cut(s)	30, 39, 2456, 3252, 3915
TaiI	ACGT	8 cut(s)	122, 1711, 2186, 2229, 2631, 2687, 2930, 3679
TaqI	TCGA	9 cut(s)	129, 264, 403, 1012, 1421, 2166, 2678, 3794, 3825
TatI	WGTACW	7 cut(s)	1138, 1507, 3033, 3263, 3518, 3738, 3763
TauI	GCSGC	4 cut(s)	206, 305, 803, 1953
TscAI	CASTG	7 cut(s)	198, 1287, 1756, 2826, 3340, 3423, 3944
TseFI	GTSAC	7 cut(s)	115, 424, 1551, 1686, 2263, 3173, 3256
Tsp45I	GTSAC	7 cut(s)	115, 424, 1551, 1686, 2263, 3173, 3256
TspGWI	ACGGA	2 cut(s)	572, 2729
TspRI	CASTG	7 cut(s)	198, 1287, 1756, 2826, 3340, 3423, 3944
Van91I	CCANNNNNTGG	3 cut(s)	518, 2518, 4075
Vha464I	CTTAAG	1 cut(s)	2251
VneI	GTGCAC	1 cut(s)	3681
VpaK11BI	GGWCC	8 cut(s)	1017, 1384, 2348, 2887, 3055, 3496, 3608, 3732
VspI	ATTAAT	2 cut(s)	633, 3695
XceI	RCATGY	4 cut(s)	546, 1092, 1733, 1768
XcmI	CCANNNNNNNNNTGG	3 cut(s)	46, 882, 2397
XmiI	GTMKAC	3 cut(s)	129, 560, 3458
XmnI	GAANNNNTTC	2 cut(s)	2475, 3136
ZraI	GACGTC	1 cut(s)	120
ZrmI	AGTACT	3 cut(s)	3265, 3520, 3740
Zsp2I	ATGCAT	1 cut(s)	548

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.