Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0001111

Physical Location & Seq

Genomic Coordinates Forward (+)

20669037 .. 20679045

Gene Structure 10009 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000012628

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4320 bp

ATGGAGGAAGCGCTTGAACTGGCACGCGCCAAGGACACCAAGGAGCGCATGGCCGGAGTGGAGCGCCTCCACCAGCTTCTCGAGGCTTCCAGGAAGAGCCTCACTTCGGCCGAGGTCACTGCGCTCGTCGATTGCTGCATGGATCTTCTCAAGGACAACAACTTCCGAGTCTCCCAGGGCGCTCTTCAGGCCCTCGCCTCCGCCGCGGTCCTCTCCGGCGACCATCTCAAGCTTCATTTCAATGCGCTTGTCCCCGCCGTCGTCGAGCGCCTCGGCGATGCCAAGCAGCCCGTTCGCGACGCCGCCCGGAGGCTCCTGCTTACTCTTATGGAGGTTTCTTCTCCAACGCTCATTGTTGAAAGAGCAGGTTCTTATGCCTGGGCACATAAAAGTTGGAGAGTTAGAGAGGAGTTTGCACGAACCGTCACCTCAGCAATTGGACTTTTTGCATCAACTGAACTTCCTCTTCAGCGTGCTATACTTCCTCCGATTTTGCAGATGTTAAGTGATTCAAATCCCGGTGTAAGGGACGCAGCTATATTGTGCATTGAGGAGATGTATACGCAGGCAGGGACTCAGTTCCGTGATGAACTTCAGCGGCACCATCTTCCCATGTCCATGGTGAAGGATATTAATGCAAGGCTAGAGAGGATTGAACCTAAGAATCGCTCATCAGATGGACTTGCTGCTGTAGAAACAAAGCCCTTGAGCCATAACCCAAAGAGAAATAGTCCAAAGGCTAAGAGTTCCTCAAGGGAGGTATCTCTATTTGGAGCGGAAACTGATCCAACTGTAAAATCTGTAGACCCCATAAAAGTTTATTCTGAAAAGGAACTAATAAGAGAAGTGGAGAAAATAGCTTCTACCCTTGTACCAGAGAAAGACTGGTCAATTCGGATTGCTGCAATGCAGAGAATAGAAGGGCTTGTATATGGAGGTGCTGCTGATTATCAATCATTTCGGGGACTTTTGAAGCAACTTGTGAATCCTCTGAACACACAGTTATCTGATCGAAGGTCTAGCATAGTGAAGCAGGCTTGCCATCTGTTATGCTTTTTGTCGAAGGAGCTATTGGGAGATTTTGAGGCATATGCAGAGATTTTCATTCCAGTCCTTTTCAAGCTGGTTGTGATCACTGTACTTGTAATTGCAGAGTCTGCAGATAACTGCATAAAAACGATGTTGCGTAACTGTAAAGTTGCTCGGGTGCTCCCTCGTATTGCGGATTGTGCAAAGAATGACCGTAATGCAATACTTCGAGCAAGGTGCTGTGACTATGCACTGTTGATACTGGAGTATTGGGCCGATGAACCTGAAATACAGAGATCGGCAGATTTGTATGAAGATCTAATAAGATGTTGTGTTGCAGATGCAATGAGCGAGGTCCGTTCAACTGCAAGAATGTGCTATAGAATGTTTTCAAAAACCTGGCCAGAGCGTTCCCGACGACTATTTTCACTATTTGATCCCGTTATTCAAAGGTTAATCAATGAAGAGGATGGGGGGATACATAGGCGACATGCTTCTCCATCTGTTCGTGATAGGGGTACACCTGTCTCATTTACTCCCCAGCCATCTGCTTCTGCTAATCTACCTGGATATGGAACTTCGGCTATTGTTGCAATGGATAGAACTTCAAGTTTATCTTCTGGGACTTCTTTCTCTTCAGGACTTCATCTGTCACAAGCGAAGTCACATGGTAAAGGTACTGAGCGTAGTCTGGAAAGTGTGTTGCATGCAAGCAAACAGAAGGTCAGTGCAATTGAAAGCATGCTTAGAGGTTTGGAGTTGTCTGACAGACATAATTCTTCAACTCTCCGGTCATCTAGTCTGGATCTAGGAGTTGACCCTCCATCATCTCGTGATCCACCATTCCCTGCTGCTGTCCCAGCTTCTAATCATCTTTCAAACTCTCTAATGACAGATTCAACCACCTCAAGTAACAAAGGTAGTAACCGCAATGGTGGCCTGGTTTTGTCTGATATCATTACACAAATTCAAGCTTCCAAAGATTCTGCTAAATCATCATATCGCACTAATTTATCAGCTGAAACACTGCCAACTGTTTCATCCTACAACATAAAGAGGGCTTCTGAGAGAATCCACGAAAGGGGTTTCATTGAAGAGAATACCGACAGTAGGGAGGCTAGGCGAACTATGAATCATCAAGCTGATAGGCATTATTTGGACACATCTCACAGGGATGGAAACTTTAGGGATTCACATAATAACCATATCCCCAATTTTCAAAGGCCCCTCTTAAGAAAGAATGCTACAGGAAGGATATCTGCAGGCAGGAGGAGAAGTTTTGATGATAGCCAACTCTCACAGGAGATGGCAAATTATGTAGAAGGTCCAGCATCGCTAAATGATGCTCTGAGTGAAGGACTAAGTTCATCTTCAGACTGGTCAGCTAGGGTTGCTGCATTTAATTATCTTCGGTCTTTGTTGCAGCAAGGCCAAAGAGGAATTCAAGAAGTGATTCAGAATTTCGAGAAAGTAATGAAGTTGTTTTTCCAACACTTGGATGATCCGCACCACAAAGTTGCACAAGCAGCCCTTTCAACACTTGCAGACATTATTCCATCATGCCGTAAGCCCTTTGAAAGTTACATGGAAAGGATCCTACCTCATGTTTTCTCACGGTTAATTGACCCAAAGGAGTTAGTTAGGCAACCTTGCTCAACAACTTTGGTCATCGTTGGCAAGACTTACAGTGTAGATTCCCTGTTACCTGCTTTGCTTCGTTCACTAGATGAACAAAGGTCACCAAAGGCTAAATTAGCTGTTATTGAGTTTTCTATCGGTTCATTTAACAAGCATTCAGTGAATACTGAAGGTTCTGGTAATAGTGGCATCCTAAAATTATGGCTTTCGAAGTTGACACCATTGGTCCATGATAAAAACACTAAACTAAAGGAGGCAGCTATTACATGCATAATCTCTGTATACTCCCACTTTGATCCAACAGCTGTCTTGAATTTTATTCTTAGCTTGTCAGTTGAAGAACAAAATTCGTTGAGAAGAGCCTTGAAACAAAGTACTCCTCGCATCGAAGTGGACCTTATGAACTTTTTGCAAAACAAGAAAGAGCGACAGCGCAAGTCTTCTTATGATCCGTCTGATGCAGTTGGAACATCTTCCGAAGATGGATATGTTAGTGCCTCAAAGAAGAGTCATTTCTTTGGGAGGTATTCTGCTGGTTCAGTTGATGGTGATGGTGGCAGGAAGTGGAGTTCAACCCAAGAAACAGCCTTGGTCAGTGGCTCGGTTGGCCAAGCAGCTTCTGATCAGACTGGGGAAAACTTGTATCAGAATTTTGAGACTGGTTCTAACATTGATGTTCTCAATGCAAAATCCAAGGATCCAACTTACATGATCAGTGCCATGAATCAGAGCTCTGGTTCCTGGACTAGCCCTCTGGACAATGGCGAAGGTAGAGTAAATTTAGAAAATTTGCAGACTCATTCCCTGGATGTTAATGGGGTTTTGAACTCGGACCATATTGGGGCTGCTGAGAGCATTGGGCATAGTGAAGCTTCCACCGACTTGGACCAGAATCACCACCAACTTAAAGCTTTAAAGGTTAATTCCATTCCAGACTCAGGTCCCAGCATTCCTCAGATACTCCACCTGATTAGCACTGGAACTGAGGAAAGTCCTGCAGAAAGTAAGCGTGGTGCACTTCAACAGCTAATCGAAGCTTCTAAAACTAATGACCACTCTATTTGGACCAAGTACTTCAATCAAATTTTGACAGTTGTACTTGAGGTGTTGGATGATTTTGACTCCTCCATCCGGGAGCTCTCTCTTTCATTGATTATTGAAATGCTTAAGAACCAGAAAGCTGCAATGGAAGATTCTATTGAGATCGTAATTGAGAAGCTGCTGCATGTTACAAAGGACTTGGTTCCCCAGGTTTCAAATGAATCAGAGCACTGCTTGAGTATTGTTCTGTCGCAGTATGATCCATTCAGATGTTTAAGTGTCATTGTCCCTTTATTGGTAACTGAAGATGAGAAAACTCTTGTTACTTGCATAAACTGTTTGACAAAGCTTGTAGGCAGGCTTTCACAGGAGGAACTAATGGCTCAATTACCATCTTTTTTGCCTGCACTGTTCGAAGCATTTGGAAACCAGAGTGCTGATGTTCGCAAGACTGTTGTCTTCTGTTTAGTTGATATATATATCATGCTTGGAAAAGCATTCTTGCCATATCTGGAGGGGCTCAACAGCACACAGTTACGGTTGGTGACTATTTATGCTAATCGAATTTCACAGGCGAGGACAGGCACGCCAATAGACACTAACCATGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000902 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0005876 GO:0005886 GO:0005911 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007026 GO:0007049 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009653 GO:0009826 GO:0009987 GO:0010639 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0022607 GO:0030054 GO:0030865 GO:0031023 GO:0031109 GO:0031110 GO:0031111 GO:0031114 GO:0031122 GO:0031647 GO:0032502 GO:0032886 GO:0032989 GO:0033043 GO:0034453 GO:0034622 GO:0040007 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043622 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0046785 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050821 GO:0051010 GO:0051128 GO:0051129 GO:0051258 GO:0051302 GO:0051493 GO:0051494 GO:0051781 GO:0055044 GO:0060560 GO:0065003 GO:0065007 GO:0065008 GO:0070507 GO:0071840 GO:0071944 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1901879 GO:1901880 GO:1902903 GO:1902904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CLASP_N HEAT Vac14_Fab1_bd

Protein Analysis

1440

Amino Acids

159.38

Weight (kDa)

6.73

Isoelectric Point (pI)

46.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CEP104-like_TOG	PF21040	10 - 180	7e-10	Centrosomal protein CEP104-like, TOG domain
XMAP215_CLASP_TOG	PF21041	11 - 213	6.4e-10	XMAP215/Dis1/CLASP, TOG domain
HEAT_GCN1_C	PF25786	18 - 116	2.1e-06	GCN1-like, C-terminal HEAT repeats
CLASP_N	PF12348	280 - 496	3.5e-48	CLASP N terminal
CLASP_N	PF12348	796 - 987	4.1e-09	CLASP N terminal
CEP104-like_TOG	PF21040	800 - 943	1.6e-08	Centrosomal protein CEP104-like, TOG domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001111)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20190
fragaria_vesca	FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_4g18130
malus_domestica	MD05G1182800.v1.1 MD10G1170900.v1.1
prunus_persica	Prupe.8G209400_v2.0.a1 Prupe.8G209400_v2.0.a1 Prupe.8G209400_v2.0.a1
pyrus_communis	pycom05g16790 pycom10g14730
rosa_chinensis	RchiOBHm_Chr1g0330771 RchiOBHm_Chr1g0330781 RchiOBHm_Chr1g0330801 RchiOBHm_Chr6g0285551
rosa_laevigata	RLG00000012628 RLG00000029789 RLG00000029790 RLG00000029796
rosa_multiflora	Rmu_co8432325.1_g000001 Rmu_sc0000723.1_g000001 Rmu_sc0000723.1_g000002 Rmu_sc0001491.1_g000006 Rmu_sc0002725.1_g000020 Rmu_sc0012495.1_g000004 Rmu_sc0017770.1_g000001 Rmu_sc0031058.1_g000001
rosa_roxburghii	Rroxscaffold_4G00319710 Rroxscaffold_4G00319750 Rroxscaffold_4G00319790 Rroxscaffold_4G00319800 Rroxscaffold_4G00319810 Rroxscaffold_7G00183030
rosa_rugosa	Rorug06G0175400
rosa_samantha	Rh1AG108200 Rh1AG109200 Rh1AG110500 Rh1AG111400 Rh1CG105100 Rh1CG105400 Rh1CG105500 Rh1CG106800 Rh2BG359900 Rh2DG376700 Rh4CG127100 Rh6AG287000 Rh6BG288200 Rh6CG289400 Rh6DG282200
rosa_wichuraiana	Rw0G004360 Rw1G008500 Rw1G008580 Rw1G008750 Rw1G008840 Rw6G024670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	356, 2743
AccB1I	GGYRCC	1 cut(s)	600
AccB7I	CCANNNNNTGG	1 cut(s)	2524
AccBSI	CCGCTC	1 cut(s)	776
AccI	GTMKAC	3 cut(s)	560, 804, 2949
AccII	CGCG	3 cut(s)	27, 206, 297
AcoI	YGGCCR	4 cut(s)	51, 108, 1430, 3274
AcuI	CTGAAG	7 cut(s)	170, 452, 578, 1650, 2385, 2856, 4032
AcyI	GRCGYC	1 cut(s)	300
AfaI	GTAC	7 cut(s)	873, 1140, 1549, 1708, 3043, 3740, 3765
AfeI	AGCGCT	1 cut(s)	12
AflII	CTTAAG	2 cut(s)	2260, 3833
AjnI	CCWGG	9 cut(s)	89, 174, 377, 1427, 1594, 1968, 3407, 3471, 3915
AjuI	GAANNNNNNNTTGG	4 cut(s)	1055, 1087, 2454, 2486
AloI	GAACNNNNNNTCC	2 cut(s)	3220, 3252
Alw21I	GWGCWC	5 cut(s)	1212, 3401, 3685, 3807, 3939
Alw26I	GTCTC	3 cut(s)	175, 1561, 3317
Alw44I	GTGCAC	1 cut(s)	3681
AlwNI	CAGNNNCTG	2 cut(s)	1157, 3287
Ama87I	CYCGRG	2 cut(s)	80, 1203
Aor51HI	AGCGCT	1 cut(s)	12
AoxI	GGCC	9 cut(s)	51, 108, 189, 1302, 1430, 1966, 2252, 2458, 3274
ApaLI	GTGCAC	1 cut(s)	3681
ArsI	GACNNNNNNTTYG	2 cut(s)	1737, 1769
AseI	ATTAAT	1 cut(s)	633
Asp700I	GAANNNNTTC	3 cut(s)	1417, 2481, 3139
AspLEI	GCGC	9 cut(s)	13, 29, 48, 66, 124, 182, 247, 270, 3102
AsuC2I	CCSGG	3 cut(s)	307, 519, 3800
AsuHPI	GGTGA	6 cut(s)	418, 634, 2760, 3227, 3554, 4264
AsuII	TTCGAA	2 cut(s)	2876, 4122
AvaI	CYCGRG	2 cut(s)	80, 1203
AvaII	GGWCC	9 cut(s)	208, 1384, 2354, 2893, 3061, 3499, 3553, 3608, 3732
BaeGI	GKGCMC	2 cut(s)	385, 3685
BaeI	ACNNNNGTAYC	6 cut(s)	855, 888, 1280, 1313, 1539, 1572
BalI	TGGCCA	2 cut(s)	1432, 3276
BamHI	GGATCC	2 cut(s)	2622, 3364
BanI	GGYRCC	1 cut(s)	600
BanII	GRGCYC	3 cut(s)	3401, 3807, 4230
BauI	CACGAG	1 cut(s)	1860
BbsI	GAAGAC	2 cut(s)	3099, 4159
Bbv12I	GWGCWC	5 cut(s)	1212, 3401, 3685, 3807, 3939
BbvCI	CCTCAGC	1 cut(s)	430
BceAI	ACGGC	2 cut(s)	242, 2577
BcgI	CGANNNNNNTGC	6 cut(s)	101, 135, 275, 309, 1248, 1282
BciT130I	CCWGG	9 cut(s)	91, 176, 379, 1429, 1596, 1970, 3409, 3473, 3917
BciVI	GTATCC	1 cut(s)	1500
BclI	TGATCA	3 cut(s)	1131, 3289, 3378
BcnI	CCSGG	3 cut(s)	307, 519, 3800
BcoDI	GTCTC	3 cut(s)	175, 1561, 3317
BfaI	CTAG	8 cut(s)	644, 1020, 1827, 1838, 2148, 2415, 2753, 3414
BfmI	CTRYAG	7 cut(s)	690, 801, 1158, 1408, 2274, 2289, 3663
BfoI	RGCGCY	4 cut(s)	14, 67, 183, 271
BfrI	CTTAAG	2 cut(s)	2260, 3833
BfuAI	ACCTGC	2 cut(s)	356, 2743
BfuI	GTATCC	1 cut(s)	1500
BglII	AGATCT	1 cut(s)	1345
BmcAI	AGTACT	2 cut(s)	3043, 3740
Bme18I	GGWCC	9 cut(s)	208, 1384, 2354, 2893, 3061, 3499, 3553, 3608, 3732
BmeT110I	CYCGRG	2 cut(s)	80, 1203
BmiI	GGNNCC	8 cut(s)	314, 602, 2255, 2624, 3366, 3406, 3610, 3912
BmrI	ACTGGG	1 cut(s)	3306
BmsI	GCATC	8 cut(s)	268, 458, 1360, 2362, 2369, 2865, 3060, 3115
BmuI	ACTGGG	1 cut(s)	3306
BoxI	GACNNNNGTC	2 cut(s)	3606, 4163
BpiI	GAAGAC	2 cut(s)	3099, 4159
BpmI	CTGGAG	2 cut(s)	1313, 4241
Bpu10I	CCTNAGC	1 cut(s)	430
Bpu14I	TTCGAA	2 cut(s)	2876, 4122
BpuEI	CTTGAG	7 cut(s)	134, 212, 727, 736, 1921, 3788, 3964
BpuMI	CCSGG	3 cut(s)	307, 519, 3800
BsaBI	GATNNNNATC	1 cut(s)	513
BsaHI	GRCGYC	1 cut(s)	300
BsaWI	WCCGGW	1 cut(s)	1818
BsaXI	ACNNNNNCTCC	2 cut(s)	2290, 2320
Bse1I	ACTGG	8 cut(s)	24, 890, 1109, 1296, 2411, 3301, 3331, 3649
Bse3DI	GCAATG	5 cut(s)	912, 1380, 1629, 1966, 3858
Bse8I	GATNNNNATC	1 cut(s)	513
BseBI	CCWGG	9 cut(s)	91, 176, 379, 1429, 1596, 1970, 3409, 3473, 3917
BseGI	GGATG	8 cut(s)	1504, 2069, 2209, 2533, 2856, 3481, 3784, 3795
BseJI	GATNNNNATC	1 cut(s)	513
BseMI	GCAATG	5 cut(s)	912, 1380, 1629, 1966, 3858
BseMII	CTCAG	9 cut(s)	444, 590, 1701, 2085, 2369, 3507, 3618, 3635, 3642
BseNI	ACTGG	8 cut(s)	24, 890, 1109, 1296, 2411, 3301, 3331, 3649
BseRI	GAGGAG	5 cut(s)	422, 566, 2314, 3036, 3781
BseSI	GKGCMC	2 cut(s)	385, 3685
BseX3I	CGGCCG	1 cut(s)	108
BseYI	CCCAGC	3 cut(s)	1569, 1888, 3611
BsgI	GTGCAG	1 cut(s)	4098
Bsh1236I	CGCG	3 cut(s)	27, 206, 297
Bsh1285I	CGRYCG	1 cut(s)	111
BshFI	GGCC	9 cut(s)	53, 110, 191, 1304, 1432, 1968, 2254, 2460, 3276
BshNI	GGYRCC	1 cut(s)	600
BsiEI	CGRYCG	1 cut(s)	111
BsiHKAI	GWGCWC	5 cut(s)	1212, 3401, 3685, 3807, 3939
BsiHKCI	CYCGRG	2 cut(s)	80, 1203
BsiSI	CCGG	6 cut(s)	54, 216, 307, 519, 1819, 3799
BslFI	GGGAC	8 cut(s)	236, 542, 586, 978, 1666, 1871, 3594, 3980
BsmAI	GTCTC	3 cut(s)	175, 1561, 3317
BsmFI	GGGAC	8 cut(s)	236, 542, 586, 978, 1666, 1871, 3594, 3980
BsmI	GAATGC	4 cut(s)	2275, 2821, 3615, 4205
BsnI	GGCC	9 cut(s)	53, 110, 191, 1304, 1432, 1968, 2254, 2460, 3276
BsoBI	CYCGRG	2 cut(s)	80, 1203
Bsp119I	TTCGAA	2 cut(s)	2876, 4122
Bsp1286I	GDGCHC	7 cut(s)	385, 1212, 3401, 3685, 3807, 3939, 4230
Bsp19I	CCATGG	1 cut(s)	618
Bsp68I	TCGCGA	1 cut(s)	297
BspANI	GGCC	9 cut(s)	53, 110, 191, 1304, 1432, 1968, 2254, 2460, 3276
BspCNI	CTCAG	9 cut(s)	443, 589, 1702, 2086, 2370, 3508, 3617, 3634, 3643
BspFNI	CGCG	3 cut(s)	27, 206, 297
BspLI	GGNNCC	8 cut(s)	314, 602, 2255, 2624, 3366, 3406, 3610, 3912
BspMAI	CTGCAG	3 cut(s)	1162, 2293, 3667
BspMI	ACCTGC	2 cut(s)	356, 2743
BspQI	GCTCTTC	3 cut(s)	89, 189, 3019
BspT104I	TTCGAA	2 cut(s)	2876, 4122
BspT107I	GGYRCC	1 cut(s)	600
BspTI	CTTAAG	2 cut(s)	2260, 3833
BsrBI	CCGCTC	1 cut(s)	776
BsrDI	GCAATG	5 cut(s)	912, 1380, 1629, 1966, 3858
BsrI	ACTGG	8 cut(s)	24, 890, 1109, 1296, 2411, 3301, 3331, 3649
BssNAI	GTATAC	2 cut(s)	561, 2950
BssNI	GRCGYC	1 cut(s)	300
BssSI	CACGAG	1 cut(s)	1860
BssT1I	CCWWGG	5 cut(s)	30, 39, 618, 3255, 3360
Bst1107I	GTATAC	2 cut(s)	561, 2950
Bst2BI	CACGAG	1 cut(s)	1860
Bst2UI	CCWGG	9 cut(s)	91, 176, 379, 1429, 1596, 1970, 3409, 3473, 3917
Bst6I	CTCTTC	8 cut(s)	89, 189, 471, 1488, 1669, 2118, 3019, 3167
BstACI	GRCGYC	1 cut(s)	300
BstAFI	CTTAAG	2 cut(s)	2260, 3833
BstAPI	GCANNNNNTGC	1 cut(s)	1157
BstBI	TTCGAA	2 cut(s)	2876, 4122
BstDSI	CCRYGG	2 cut(s)	204, 618
BstEII	GGTNACC	1 cut(s)	2766
BstF5I	GGATG	8 cut(s)	1504, 2069, 2209, 2533, 2856, 3481, 3784, 3795
BstFNI	CGCG	3 cut(s)	27, 206, 297
BstH2I	RGCGCY	4 cut(s)	14, 67, 183, 271
BstHHI	GCGC	9 cut(s)	13, 29, 48, 66, 124, 182, 247, 270, 3102
BstMAI	GTCTC	3 cut(s)	175, 1561, 3317
BstMCI	CGRYCG	1 cut(s)	111
BstNI	CCWGG	9 cut(s)	91, 176, 379, 1429, 1596, 1970, 3409, 3473, 3917
BstNSI	RCATGY	5 cut(s)	1523, 1739, 1774, 2937, 3896
BstPAI	GACNNNNGTC	2 cut(s)	3606, 4163
BstPI	GGTNACC	1 cut(s)	2766
BstSFI	CTRYAG	7 cut(s)	690, 801, 1158, 1408, 2274, 2289, 3663
BstSLI	GKGCMC	2 cut(s)	385, 3685
BstUI	CGCG	3 cut(s)	27, 206, 297
BstV2I	GAAGAC	2 cut(s)	3099, 4159
BstX2I	RGATCY	5 cut(s)	142, 1345, 1834, 2622, 3364
BstXI	CCANNNNNNTGG	2 cut(s)	619, 3550
BstYI	RGATCY	5 cut(s)	142, 1345, 1834, 2622, 3364
BstZ17I	GTATAC	2 cut(s)	561, 2950
BstZI	CGGCCG	1 cut(s)	108
BsuI	GTATCC	1 cut(s)	1500
BsuRI	GGCC	9 cut(s)	53, 110, 191, 1304, 1432, 1968, 2254, 2460, 3276
BtgI	CCRYGG	2 cut(s)	204, 618
BtgZI	GCGATG	2 cut(s)	291, 2346
BtsCI	GGATG	8 cut(s)	1504, 2069, 2209, 2533, 2856, 3481, 3784, 3795
BtsI	GCAGTG	3 cut(s)	117, 2054, 3937
BtuMI	TCGCGA	1 cut(s)	297
BveI	ACCTGC	2 cut(s)	356, 2743
CaiI	CAGNNNCTG	2 cut(s)	1157, 3287
CfoI	GCGC	9 cut(s)	13, 29, 48, 66, 124, 182, 247, 270, 3102
Cfr42I	CCGCGG	1 cut(s)	207
CseI	GACGC	2 cut(s)	308, 539
Csp6I	GTAC	7 cut(s)	872, 1139, 1548, 1707, 3042, 3739, 3764
CspCI	CAANNNNNGTGG	2 cut(s)	3710, 3745
CviQI	GTAC	7 cut(s)	872, 1139, 1548, 1707, 3042, 3739, 3764
DraI	TTTAAA	1 cut(s)	3582
EaeI	YGGCCR	4 cut(s)	51, 108, 1430, 3274
EagI	CGGCCG	1 cut(s)	108
Eam1104I	CTCTTC	8 cut(s)	89, 189, 471, 1488, 1669, 2118, 3019, 3167
EarI	CTCTTC	8 cut(s)	89, 189, 471, 1488, 1669, 2118, 3019, 3167
EciI	GGCGGA	1 cut(s)	190
Ecl136II	GAGCTC	2 cut(s)	3399, 3805
EclXI	CGGCCG	1 cut(s)	108
Eco130I	CCWWGG	5 cut(s)	30, 39, 618, 3255, 3360
Eco24I	GRGCYC	3 cut(s)	3401, 3807, 4230
Eco32I	GATATC	2 cut(s)	1984, 2286
Eco47I	GGWCC	9 cut(s)	208, 1384, 2354, 2893, 3061, 3499, 3553, 3608, 3732
Eco47III	AGCGCT	1 cut(s)	12
Eco52I	CGGCCG	1 cut(s)	108
Eco53kI	GAGCTC	2 cut(s)	3399, 3805
Eco57I	CTGAAG	7 cut(s)	170, 452, 578, 1650, 2385, 2856, 4032
Eco88I	CYCGRG	2 cut(s)	80, 1203
Eco91I	GGTNACC	1 cut(s)	2766
EcoICRI	GAGCTC	2 cut(s)	3399, 3805
EcoO109I	RGGNCCY	3 cut(s)	190, 2253, 3608
EcoO65I	GGTNACC	1 cut(s)	2766
EcoRI	GAATTC	1 cut(s)	2469
EcoRII	CCWGG	9 cut(s)	89, 174, 377, 1427, 1594, 1968, 3407, 3471, 3915
EcoRV	GATATC	2 cut(s)	1984, 2286
EcoT14I	CCWWGG	5 cut(s)	30, 39, 618, 3255, 3360
EcoT22I	ATGCAT	1 cut(s)	2939
EcoT38I	GRGCYC	3 cut(s)	3401, 3807, 4230
ErhI	CCWWGG	5 cut(s)	30, 39, 618, 3255, 3360
FalI	AAGNNNNNCTT	8 cut(s)	1630, 1662, 2383, 2415, 2544, 2576, 3048, 3080
FaqI	GGGAC	8 cut(s)	236, 542, 586, 978, 1666, 1871, 3594, 3980
FauI	CCCGC	1 cut(s)	262
FauNDI	CATATG	1 cut(s)	1090
FbaI	TGATCA	3 cut(s)	1131, 3289, 3378
FblI	GTMKAC	3 cut(s)	560, 804, 2949
FokI	GGATG	8 cut(s)	1511, 2056, 2216, 2540, 2843, 3488, 3782, 3791
FriOI	GRGCYC	3 cut(s)	3401, 3807, 4230
FspBI	CTAG	8 cut(s)	644, 1020, 1827, 1838, 2148, 2415, 2753, 3414
GlaI	GCGC	9 cut(s)	12, 28, 47, 65, 123, 181, 246, 269, 3101
GsaI	CCCAGC	3 cut(s)	1573, 1892, 3615
GsuI	CTGGAG	2 cut(s)	1313, 4241
HaeII	RGCGCY	4 cut(s)	14, 67, 183, 271
HaeIII	GGCC	9 cut(s)	53, 110, 191, 1304, 1432, 1968, 2254, 2460, 3276
HapII	CCGG	6 cut(s)	54, 216, 307, 519, 1819, 3799
HgaI	GACGC	2 cut(s)	308, 539
HhaI	GCGC	9 cut(s)	13, 29, 48, 66, 124, 182, 247, 270, 3102
Hin1I	GRCGYC	1 cut(s)	300
Hin6I	GCGC	9 cut(s)	11, 27, 46, 64, 122, 180, 245, 268, 3100
HinP1I	GCGC	9 cut(s)	11, 27, 46, 64, 122, 180, 245, 268, 3100
HincII	GTYRAC	2 cut(s)	1846, 2883
HindII	GTYRAC	2 cut(s)	1846, 2883
HindIII	AAGCTT	6 cut(s)	230, 2001, 3537, 3576, 3702, 4055
HpaII	CCGG	6 cut(s)	54, 216, 307, 519, 1819, 3799
HphI	GGTGA	6 cut(s)	418, 634, 2760, 3227, 3554, 4264
Hpy166II	GTNNAC	9 cut(s)	561, 805, 1550, 1846, 2750, 2883, 2950, 3061, 3683
Hpy8I	GTNNAC	9 cut(s)	561, 805, 1550, 1846, 2750, 2883, 2950, 3061, 3683
Hpy99I	CGWCG	5 cut(s)	131, 263, 266, 302, 1449
Hsp92I	GRCGYC	1 cut(s)	300
HspAI	GCGC	9 cut(s)	11, 27, 46, 64, 122, 180, 245, 268, 3100
Ksp22I	TGATCA	3 cut(s)	1131, 3289, 3378
KspI	CCGCGG	1 cut(s)	207
LguI	GCTCTTC	3 cut(s)	89, 189, 3019
LmnI	GCTCC	7 cut(s)	43, 61, 318, 773, 1066, 1215, 3802
LweI	GCATC	8 cut(s)	268, 458, 1360, 2362, 2369, 2865, 3060, 3115
MaeI	CTAG	8 cut(s)	644, 1020, 1827, 1838, 2148, 2415, 2753, 3414
MbiI	CCGCTC	1 cut(s)	776
MfeI	CAATTG	2 cut(s)	435, 1761
MflI	RGATCY	5 cut(s)	142, 1345, 1834, 2622, 3364
MhlI	GDGCHC	7 cut(s)	385, 1212, 3401, 3685, 3807, 3939, 4230
MlsI	TGGCCA	2 cut(s)	1432, 3276
MluNI	TGGCCA	2 cut(s)	1432, 3276
MlyI	GAGTC	7 cut(s)	177, 568, 1163, 3184, 3457, 3596, 3782
MmeI	TCCRAC	8 cut(s)	368, 374, 812, 2542, 2990, 3112, 3392, 3756
Mox20I	TGGCCA	2 cut(s)	1432, 3276
Mph1103I	ATGCAT	1 cut(s)	2939
MroXI	GAANNNNTTC	3 cut(s)	1417, 2481, 3139
MscI	TGGCCA	2 cut(s)	1432, 3276
MslI	CAYNNNNRTG	6 cut(s)	240, 617, 2202, 2526, 3056, 3976
Msp20I	TGGCCA	2 cut(s)	1432, 3276
MspA1I	CMGCKG	4 cut(s)	206, 598, 2048, 2972
MspCI	CTTAAG	2 cut(s)	2260, 3833
MspI	CCGG	6 cut(s)	54, 216, 307, 519, 1819, 3799
MunI	CAATTG	2 cut(s)	435, 1761
Mva1269I	GAATGC	4 cut(s)	2275, 2821, 3615, 4205
MvaI	CCWGG	9 cut(s)	91, 176, 379, 1429, 1596, 1970, 3409, 3473, 3917
MvnI	CGCG	3 cut(s)	27, 206, 297
NciI	CCSGG	3 cut(s)	307, 519, 3800
NcoI	CCATGG	1 cut(s)	618
NdeI	CATATG	1 cut(s)	1090
NlaIV	GGNNCC	8 cut(s)	314, 602, 2255, 2624, 3366, 3406, 3610, 3912
NmeAIII	GCCGAG	2 cut(s)	136, 252
NmuCI	GTSAC	7 cut(s)	115, 424, 1271, 1680, 1692, 2766, 4252
NruI	TCGCGA	1 cut(s)	297
NsiI	ATGCAT	1 cut(s)	2939
NspI	RCATGY	5 cut(s)	1523, 1739, 1774, 2937, 3896
NspV	TTCGAA	2 cut(s)	2876, 4122
PaeI	GCATGC	2 cut(s)	1739, 1774
PaeR7I	CTCGAG	1 cut(s)	80
PasI	CCCWGGG	1 cut(s)	175
PciSI	GCTCTTC	3 cut(s)	89, 189, 3019
PctI	GAATGC	4 cut(s)	2275, 2821, 3615, 4205
PdmI	GAANNNNTTC	3 cut(s)	1417, 2481, 3139
PflMI	CCANNNNNTGG	1 cut(s)	2524
PfoI	TCCNGGA	3 cut(s)	89, 3407, 3798
PleI	GAGTC	7 cut(s)	176, 568, 1162, 3183, 3457, 3596, 3782
PpsI	GAGTC	7 cut(s)	176, 568, 1162, 3183, 3457, 3596, 3782
PpuMI	RGGWCCY	1 cut(s)	3608
PshAI	GACNNNNGTC	2 cut(s)	3606, 4163
PshBI	ATTAAT	1 cut(s)	633
Psp124BI	GAGCTC	2 cut(s)	3401, 3807
Psp5II	RGGWCCY	1 cut(s)	3608
Psp6I	CCWGG	9 cut(s)	89, 174, 377, 1427, 1594, 1968, 3407, 3471, 3915
PspEI	GGTNACC	1 cut(s)	2766
PspFI	CCCAGC	3 cut(s)	1569, 1888, 3611
PspGI	CCWGG	9 cut(s)	89, 174, 377, 1427, 1594, 1968, 3407, 3471, 3915
PspN4I	GGNNCC	8 cut(s)	314, 602, 2255, 2624, 3366, 3406, 3610, 3912
PspPPI	RGGWCCY	1 cut(s)	3608
PsrI	GAACNNNNNNTAC	2 cut(s)	1302, 1334
PstI	CTGCAG	3 cut(s)	1162, 2293, 3667
PstNI	CAGNNNCTG	2 cut(s)	1157, 3287
PsuI	RGATCY	5 cut(s)	142, 1345, 1834, 2622, 3364
PvuII	CAGCTG	2 cut(s)	2048, 2972
RruI	TCGCGA	1 cut(s)	297
RsaI	GTAC	7 cut(s)	873, 1140, 1549, 1708, 3043, 3740, 3765
RsaNI	GTAC	7 cut(s)	872, 1139, 1548, 1707, 3042, 3739, 3764
RseI	CAYNNNNRTG	6 cut(s)	240, 617, 2202, 2526, 3056, 3976
SacI	GAGCTC	2 cut(s)	3401, 3807
SacII	CCGCGG	1 cut(s)	207
SapI	GCTCTTC	3 cut(s)	89, 189, 3019
ScaI	AGTACT	2 cut(s)	3043, 3740
SchI	GAGTC	7 cut(s)	177, 568, 1163, 3184, 3457, 3596, 3782
SduI	GDGCHC	7 cut(s)	385, 1212, 3401, 3685, 3807, 3939, 4230
SfaNI	GCATC	8 cut(s)	268, 458, 1360, 2362, 2369, 2865, 3060, 3115
SfcI	CTRYAG	7 cut(s)	690, 801, 1158, 1408, 2274, 2289, 3663
Sfr274I	CTCGAG	1 cut(s)	80
Sfr303I	CCGCGG	1 cut(s)	207
SfuI	TTCGAA	2 cut(s)	2876, 4122
SgrBI	CCGCGG	1 cut(s)	207
SinI	GGWCC	9 cut(s)	208, 1384, 2354, 2893, 3061, 3499, 3553, 3608, 3732
SlaI	CTCGAG	1 cut(s)	80
SmiMI	CAYNNNNRTG	6 cut(s)	240, 617, 2202, 2526, 3056, 3976
SphI	GCATGC	2 cut(s)	1739, 1774
SspMI	CTAG	8 cut(s)	644, 1020, 1827, 1838, 2148, 2415, 2753, 3414
SstI	GAGCTC	2 cut(s)	3401, 3807
StyI	CCWWGG	5 cut(s)	30, 39, 618, 3255, 3360
TaqII	GACCGA	1 cut(s)	2430
TatI	WGTACW	4 cut(s)	1138, 3041, 3738, 3763
TauI	GCSGC	3 cut(s)	206, 305, 601
TseFI	GTSAC	7 cut(s)	115, 424, 1271, 1680, 1692, 2766, 4252
Tsp45I	GTSAC	7 cut(s)	115, 424, 1271, 1680, 1692, 2766, 4252
TspGWI	ACGGA	3 cut(s)	572, 1376, 3108
Van91I	CCANNNNNTGG	1 cut(s)	2524
Vha464I	CTTAAG	2 cut(s)	2260, 3833
VneI	GTGCAC	1 cut(s)	3681
VpaK11BI	GGWCC	9 cut(s)	208, 1384, 2354, 2893, 3061, 3499, 3553, 3608, 3732
VspI	ATTAAT	1 cut(s)	633
XceI	RCATGY	5 cut(s)	1523, 1739, 1774, 2937, 3896
XcmI	CCANNNNNNNNNTGG	2 cut(s)	46, 882
XhoI	CTCGAG	1 cut(s)	80
XmiI	GTMKAC	3 cut(s)	560, 804, 2949
XmnI	GAANNNNTTC	3 cut(s)	1417, 2481, 3139
XspI	CTAG	8 cut(s)	644, 1020, 1827, 1838, 2148, 2415, 2753, 3414
ZrmI	AGTACT	2 cut(s)	3043, 3740
Zsp2I	ATGCAT	1 cut(s)	2939

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.