Rroxscaffold_4G00319750

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001111
Physical Location & Seq
Forward (+)
47923890 .. 47924249
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00319750.1

Sequence Viewer

Length: 360 bp
ATGCGTAGCTATCCGAATGGGGATTTTTGGAAAGATGAATACGACAAGCATGGAACATGCACTGTTAAAAAATTTCCTACACCTACCGAGTACTTCCAGAAGGCAAATGACTTGTGGATAGCCAACCCAGTAGAGGATTGGTTTCAGCGTGATGGTCGCTTCACAGATACAGATGTCCAGCTCGCTGAGCTGAGAGGCGCGATCATGAACGAGTTCGGTTCTGTGCCATGGTTTCAGTGTCAGGACAAATTTGTGCGGCAGATAGGCTTGTGTTTTGATGAGGGTGGAGGGCAGGCTGCGAATTGTCCTAAAGAATATCAAAAGAATGAATGTCCCGTTACTGTACAGTATAGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

119

Amino Acids

13.9

Weight (kDa)

4.95

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 6 - 102 2.9e-15 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 200
AciI CCGC 1 cut(s) 256
AcsI RAATTY 2 cut(s) 71, 248
AfaI GTAC 2 cut(s) 92, 345
AfiI CCNNNNNNNGG 1 cut(s) 133
AluBI AGCT 3 cut(s) 9, 181, 190
AluI AGCT 3 cut(s) 9, 181, 190
ApeKI GCWGC 1 cut(s) 296
ApoI RAATTY 2 cut(s) 71, 248
Asp700I GAANNNNTTC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 200
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BbvI GCAGC 1 cut(s) 283
BccI CCATC 1 cut(s) 146
BisI GCNGC 2 cut(s) 257, 297
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 2 cut(s) 258, 298
BmcAI AGTACT 1 cut(s) 92
BmrI ACTGGG 1 cut(s) 122
BmuI ACTGGG 1 cut(s) 122
Bpu1102I GCTNAGC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 227
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 128
BseDI CCNNGG 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMII CTCAG 2 cut(s) 177, 182
BseNI ACTGG 1 cut(s) 128
BseXI GCAGC 1 cut(s) 283
Bsh1236I CGCG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 318
BslI CCNNNNNNNGG 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 318
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 1 cut(s) 201
Bsp1720I GCTNAGC 1 cut(s) 186
Bsp19I CCATGG 1 cut(s) 227
BspACI CCGC 1 cut(s) 256
BspCNI CTCAG 2 cut(s) 178, 183
BspFNI CGCG 1 cut(s) 200
BspHI TCATGA 1 cut(s) 204
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 1 cut(s) 128
BssECI CCNNGG 1 cut(s) 227
BssMI GATC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 227
Bst4CI ACNGT 3 cut(s) 64, 343, 348
BstAUI TGTACA 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 183, 294
BstDEI CTNAG 2 cut(s) 186, 191
BstDSI CCRYGG 1 cut(s) 227
BstFNI CGCG 1 cut(s) 200
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 1 cut(s) 204
BstMBI GATC 1 cut(s) 201
BstMWI GCNNNNNNNGC 1 cut(s) 187
BstNSI RCATGY 1 cut(s) 60
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 1 cut(s) 283
BtgI CCRYGG 1 cut(s) 227
BtsIMutI CAGTG 2 cut(s) 60, 242
Cac8I GCNNGC 2 cut(s) 183, 294
CciI TCATGA 1 cut(s) 204
CfoI GCGC 1 cut(s) 200
Csp6I GTAC 2 cut(s) 91, 344
CviAII CATG 4 cut(s) 50, 57, 205, 228
CviJI RGCY 6 cut(s) 9, 122, 181, 190, 267, 296
CviKI_1 RGCY 6 cut(s) 9, 122, 181, 190, 267, 296
CviQI GTAC 2 cut(s) 91, 344
DdeI CTNAG 2 cut(s) 186, 191
DpnI GATC 1 cut(s) 203
DpnII GATC 1 cut(s) 201
Eco130I CCWWGG 1 cut(s) 227
EcoT14I CCWWGG 1 cut(s) 227
ErhI CCWWGG 1 cut(s) 227
FaeI CATG 4 cut(s) 53, 60, 208, 231
FaiI YATR 5 cut(s) 51, 58, 206, 229, 351
FaqI GGGAC 1 cut(s) 318
FatI CATG 4 cut(s) 49, 56, 204, 227
Fnu4HI GCNGC 2 cut(s) 257, 297
Fsp4HI GCNGC 2 cut(s) 257, 297
GlaI GCGC 1 cut(s) 199
GluI GCNGC 2 cut(s) 257, 297
HhaI GCGC 1 cut(s) 200
Hin1II CATG 4 cut(s) 53, 60, 208, 231
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 3 cut(s) 97, 205, 242
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 3 cut(s) 64, 343, 348
HpyCH4V TGCA 1 cut(s) 60
HpyF10VI GCNNNNNNNGC 1 cut(s) 187
HpyF3I CTNAG 2 cut(s) 186, 191
Hsp92II CATG 4 cut(s) 53, 60, 208, 231
HspAI GCGC 1 cut(s) 198
Kzo9I GATC 1 cut(s) 201
LpnPI CCDG 5 cut(s) 110, 141, 191, 227, 278
Lsp1109I GCAGC 1 cut(s) 283
MaeIII GTNAC 1 cut(s) 337
MalI GATC 1 cut(s) 203
MboI GATC 1 cut(s) 201
MluCI AATT 3 cut(s) 71, 248, 301
MnlI CCTC 4 cut(s) 127, 188, 274, 281
MroXI GAANNNNTTC 1 cut(s) 212
MseI TTAA 1 cut(s) 66
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 1 cut(s) 187
NcoI CCATGG 1 cut(s) 227
NdeII GATC 1 cut(s) 201
NlaIII CATG 4 cut(s) 53, 60, 208, 231
NspI RCATGY 1 cut(s) 60
PagI TCATGA 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 212
PkrI GCNGC 2 cut(s) 258, 298
RsaI GTAC 2 cut(s) 92, 345
RsaNI GTAC 2 cut(s) 91, 344
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 2 cut(s) 257, 297
Sau3AI GATC 1 cut(s) 201
ScaI AGTACT 1 cut(s) 92
SetI ASST 4 cut(s) 11, 85, 183, 192
Sse9I AATT 3 cut(s) 71, 248, 301
SsiI CCGC 1 cut(s) 256
StyI CCWWGG 1 cut(s) 227
TaaI ACNGT 3 cut(s) 64, 343, 348
TasI AATT 3 cut(s) 71, 248, 301
TatI WGTACW 2 cut(s) 90, 343
TauI GCSGC 1 cut(s) 259
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TscAI CASTG 2 cut(s) 67, 242
TseI GCWGC 1 cut(s) 296
TspDTI ATGAA 3 cut(s) 51, 221, 342
TspRI CASTG 2 cut(s) 67, 242
XapI RAATTY 2 cut(s) 71, 248
XceI RCATGY 1 cut(s) 60
XcmI CCANNNNNNNNNTGG 1 cut(s) 135
XmnI GAANNNNTTC 1 cut(s) 212
ZrmI AGTACT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.