RchiOBHm_Chr1g0330801

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001111
Physical Location & Seq
Reverse (-)
20527975 .. 20528985
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55992

Sequence Viewer

Length: 579 bp
ATGATTAATACGAGAGCAAAAGAATCTCGTTTCACTCTTCACGGGCTTTGGCCCTTCAACGCCACTCAATTAGATCCAAACAATATTAATTCTGATTTCCAGTATAGACCTATCATAGAGGGTGCCCAGGTTCCTAGTGCCCAGGTTCCTACTAGCTTTCAGGCATCACAGTTACCTATTGTCTTACTATATAAACTCGCAAAATCTTGGAAAAGTTATATGCGTAGCTATCCGAATGGGGATTTTTGGAAAGATGAATACGACAAGCATGGAACATGCACCGTTAAAAAATTTCGTACACCTGTCGAGTACTTCCAGAAGGCAAATGACTTGTGGGAAGCCAACCCAATAGAGGATTGGTTTCGGCGTGATGGTCGCTTCACAGATACAGATGTCCAGCTCGCTGAGCTGAGAGGCGCGATCATGAACAAGTTCGGTTCTGTGCCATGGTTTCAGTGTGAGGACAAATTTGTGCGGCAGATAGGCTTGTGTTTTGATGAGGTTGGAGGGTTGGGTGCGAATTGTCCTAAAGAATATCAAAAGAAGCAATGCCCCCCTACTATACAGTATAGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

192

Amino Acids

22.38

Weight (kDa)

8.54

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 6 - 167 2.3e-24 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AccII CGCG 1 cut(s) 419
AciI CCGC 1 cut(s) 475
AclWI GGATC 1 cut(s) 68
AcsI RAATTY 2 cut(s) 290, 467
AfaI GTAC 2 cut(s) 298, 311
AfiI CCNNNNNNNGG 1 cut(s) 352
AgsI TTSAA 1 cut(s) 58
AjnI CCWGG 2 cut(s) 126, 141
AluBI AGCT 4 cut(s) 156, 228, 400, 409
AluI AGCT 4 cut(s) 156, 228, 400, 409
AlwI GGATC 1 cut(s) 68
AoxI GGCC 1 cut(s) 50
ApoI RAATTY 2 cut(s) 290, 467
AseI ATTAAT 2 cut(s) 6, 87
Asp700I GAANNNNTTC 1 cut(s) 431
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 51
BaeGI GKGCMC 2 cut(s) 127, 142
BaeI ACNNNNGTAYC 2 cut(s) 378, 411
BanI GGYRCC 1 cut(s) 122
BccI CCATC 1 cut(s) 365
BciT130I CCWGG 2 cut(s) 128, 143
BfaI CTAG 2 cut(s) 135, 153
BisI GCNGC 1 cut(s) 476
BlpI GCTNAGC 1 cut(s) 405
BlsI GCNGC 1 cut(s) 477
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 2 cut(s) 128, 143
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 3 cut(s) 124, 132, 147
BmrFI CCNGG 2 cut(s) 128, 143
BmsI GCATC 1 cut(s) 173
Bpu1102I GCTNAGC 1 cut(s) 405
BsaJI CCNNGG 3 cut(s) 126, 141, 446
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse1I ACTGG 1 cut(s) 100
Bse3DI GCAATG 1 cut(s) 554
BseBI CCWGG 2 cut(s) 128, 143
BseDI CCNNGG 3 cut(s) 126, 141, 446
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMI GCAATG 1 cut(s) 554
BseMII CTCAG 2 cut(s) 396, 401
BseNI ACTGG 1 cut(s) 100
BseSI GKGCMC 2 cut(s) 127, 142
Bsh1236I CGCG 1 cut(s) 419
BshFI GGCC 1 cut(s) 52
BshNI GGYRCC 1 cut(s) 122
BslI CCNNNNNNNGG 1 cut(s) 352
BsnI GGCC 1 cut(s) 52
Bsp1286I GDGCHC 2 cut(s) 127, 142
Bsp143I GATC 2 cut(s) 73, 420
Bsp1720I GCTNAGC 1 cut(s) 405
Bsp19I CCATGG 1 cut(s) 446
BspACI CCGC 1 cut(s) 475
BspANI GGCC 1 cut(s) 52
BspCNI CTCAG 2 cut(s) 397, 402
BspFNI CGCG 1 cut(s) 419
BspHI TCATGA 1 cut(s) 423
BspLI GGNNCC 3 cut(s) 124, 132, 147
BspPI GGATC 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 554
BsrI ACTGG 1 cut(s) 100
BssECI CCNNGG 3 cut(s) 126, 141, 446
BssMI GATC 2 cut(s) 73, 420
BssT1I CCWWGG 1 cut(s) 446
Bst2UI CCWGG 2 cut(s) 128, 143
Bst4CI ACNGT 3 cut(s) 171, 283, 567
Bst6I CTCTTC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 2 cut(s) 405, 410
BstDSI CCRYGG 1 cut(s) 446
BstFNI CGCG 1 cut(s) 419
BstHHI GCGC 1 cut(s) 419
BstKTI GATC 2 cut(s) 76, 423
BstMBI GATC 2 cut(s) 73, 420
BstMWI GCNNNNNNNGC 1 cut(s) 406
BstNI CCWGG 2 cut(s) 128, 143
BstNSI RCATGY 1 cut(s) 279
BstSCI CCNGG 2 cut(s) 126, 141
BstSLI GKGCMC 2 cut(s) 127, 142
BstUI CGCG 1 cut(s) 419
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 1 cut(s) 52
BtgI CCRYGG 1 cut(s) 446
BtsIMutI CAGTG 1 cut(s) 461
Cac8I GCNNGC 1 cut(s) 402
CciI TCATGA 1 cut(s) 423
CfoI GCGC 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 51
Csp6I GTAC 2 cut(s) 297, 310
CviAII CATG 4 cut(s) 269, 276, 424, 447
CviJI RGCY 8 cut(s) 46, 52, 156, 228, 341, 400, 409, 486
CviKI_1 RGCY 8 cut(s) 46, 52, 156, 228, 341, 400, 409, 486
CviQI GTAC 2 cut(s) 297, 310
DdeI CTNAG 2 cut(s) 405, 410
DpnI GATC 2 cut(s) 75, 422
DpnII GATC 2 cut(s) 73, 420
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 446
EcoRII CCWGG 2 cut(s) 126, 141
EcoT14I CCWWGG 1 cut(s) 446
ErhI CCWWGG 1 cut(s) 446
FaeI CATG 4 cut(s) 272, 279, 427, 450
FatI CATG 4 cut(s) 268, 275, 423, 446
Fnu4HI GCNGC 1 cut(s) 476
Fsp4HI GCNGC 1 cut(s) 476
FspBI CTAG 2 cut(s) 135, 153
GlaI GCGC 1 cut(s) 418
GluI GCNGC 1 cut(s) 476
HaeIII GGCC 1 cut(s) 52
HhaI GCGC 1 cut(s) 419
Hin1II CATG 4 cut(s) 272, 279, 427, 450
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HinfI GANTC 1 cut(s) 23
Hpy166II GTNNAC 1 cut(s) 299
Hpy188I TCNGA 2 cut(s) 94, 234
Hpy188III TCNNGA 2 cut(s) 316, 424
Hpy8I GTNNAC 1 cut(s) 299
HpyAV CCTTC 2 cut(s) 64, 313
HpyCH4III ACNGT 3 cut(s) 171, 283, 567
HpyCH4V TGCA 1 cut(s) 279
HpyF10VI GCNNNNNNNGC 1 cut(s) 406
HpyF3I CTNAG 2 cut(s) 405, 410
Hsp92II CATG 4 cut(s) 272, 279, 427, 450
HspAI GCGC 1 cut(s) 417
Kzo9I GATC 2 cut(s) 73, 420
LpnPI CCDG 9 cut(s) 113, 113, 128, 140, 146, 155, 315, 329, 410
LweI GCATC 1 cut(s) 173
MaeI CTAG 2 cut(s) 135, 153
MaeIII GTNAC 1 cut(s) 171
MalI GATC 2 cut(s) 75, 422
MboI GATC 2 cut(s) 73, 420
MboII GAAGA 1 cut(s) 29
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 2 cut(s) 127, 142
MluCI AATT 5 cut(s) 68, 88, 290, 467, 520
MmeI TCCRAC 1 cut(s) 484
MnlI CCTC 6 cut(s) 112, 346, 407, 454, 493, 500
MroXI GAANNNNTTC 1 cut(s) 431
MseI TTAA 3 cut(s) 6, 87, 285
MspR9I CCNGG 2 cut(s) 128, 143
MvaI CCWGG 2 cut(s) 128, 143
MvnI CGCG 1 cut(s) 419
MwoI GCNNNNNNNGC 1 cut(s) 406
NcoI CCATGG 1 cut(s) 446
NdeII GATC 2 cut(s) 73, 420
NlaIII CATG 4 cut(s) 272, 279, 427, 450
NlaIV GGNNCC 3 cut(s) 124, 132, 147
NspI RCATGY 1 cut(s) 279
PagI TCATGA 1 cut(s) 423
PdmI GAANNNNTTC 1 cut(s) 431
PfeI GAWTC 1 cut(s) 23
PkrI GCNGC 1 cut(s) 477
PshBI ATTAAT 2 cut(s) 6, 87
Psp6I CCWGG 2 cut(s) 126, 141
PspGI CCWGG 2 cut(s) 126, 141
PspN4I GGNNCC 3 cut(s) 124, 132, 147
PspPI GGNCC 1 cut(s) 51
PsuI RGATCY 1 cut(s) 73
RsaI GTAC 2 cut(s) 298, 311
RsaNI GTAC 2 cut(s) 297, 310
SaqAI TTAA 3 cut(s) 6, 87, 285
SatI GCNGC 1 cut(s) 476
Sau3AI GATC 2 cut(s) 73, 420
Sau96I GGNCC 1 cut(s) 51
ScaI AGTACT 1 cut(s) 311
ScrFI CCNGG 2 cut(s) 128, 143
SduI GDGCHC 2 cut(s) 127, 142
SfaNI GCATC 1 cut(s) 173
Sse9I AATT 5 cut(s) 68, 88, 290, 467, 520
SsiI CCGC 1 cut(s) 475
SspI AATATT 1 cut(s) 85
SspMI CTAG 2 cut(s) 135, 153
StyD4I CCNGG 2 cut(s) 126, 141
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 3 cut(s) 171, 283, 567
TaqI TCGA 1 cut(s) 306
TasI AATT 5 cut(s) 68, 88, 290, 467, 520
TatI WGTACW 1 cut(s) 309
TauI GCSGC 1 cut(s) 478
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 3 cut(s) 6, 87, 285
Tru9I TTAA 3 cut(s) 6, 87, 285
TscAI CASTG 1 cut(s) 461
TspDTI ATGAA 2 cut(s) 270, 440
TspRI CASTG 1 cut(s) 461
VspI ATTAAT 2 cut(s) 6, 87
XapI RAATTY 2 cut(s) 290, 467
XceI RCATGY 1 cut(s) 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 354
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 2 cut(s) 135, 153
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.