Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001111

Physical Location & Seq

Genomic Coordinates Reverse (-)

26355036 .. 26364860

Gene Structure 9825 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1170900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4317 bp

ATGGAGGAAGCGCTGGAGCTCGCGCGTGCCAAGGACACCAAGGAGCGCATGGCGGGAGTGGAGCGCCTGCATCAGCTTCTGGAAGCGTCCAGAAAGAGCCTGACTTCGTCGGACGTGACGTCGCTCGTCGACTGCTGCTTGGATCTTCTCAAGGACAACAACTTCAGAGTCTCCCAGGGCGCTCTGCAGGCCCTTGCCTCCGCCGCAGTGCTCTCCGGCGACCACCTCAAGCTCCATTTCAATGCGCTTGTTCCCGCCGTCGTTGAGCGTTTGGGCGATGGCAAACAGCCCGTCCGCGACGCCGCTAGGAGGCTCCTGCTTACTCTTATGGAGGTTTCTTCTCCGACAATAATCGTTGAAAGAGCAGGATCTTATGCCTGGGCGCACAAAAGCTGGAGAGTTCGAGAAGAGTTTGCACGAACGGTCACCTCAGCAATTGGACTTTTTGCATCAACTGAACTTCCACTTCAGCGGACTATACTTCCTCCGATTCTACAGATGTTAAGTGATCCAAACCCTGGTGTAAGGGATGCAGCTATAGCGTGCATTGAGGAGATGTATACACAGGCAGGACCTCAATTCCGTGATGAACTTCAGAGGCACCACCTTCCTATGTCTATGCTGAAGGATATTAATGCAAGGTTAGAGAGGATTGAACCTAAGATTCGCTCATCAGATGGACTGAGTGCTGTAGAAGCGAAGCCTGTGAACCTTAACCACAAGAAAAGTAGTCCAAAGGCCAAGAGTTCCTCGAGGGAGGCATCTCTCTTTGGAGCGGAAACAGATGCTTCTGAAAAATCCGTAGACCCTATTAAAGTTTATTCAGAAAAGGAACTAATAAGGGAAATTGAGAAAATTGCTTCTACACTTGTACCAGAGAAAGACTGGTCAATTCGCATTGCTGCAATGCAGAGAATTGAAGGACTTGTATATGGAGGTGCTGCTGACTACCAATGTTTCCGGGGACTCCTGAAGCAACTTGTTGGGCCTCTGAGCACACAATTATCTGATCGACGGTCCAGCATAGTGAAACAGGCTTGCCATCTGTTATGCTTTTTGTCAAAGGAGTTATTAGGAGATTTTGAGGCATGTGCGGAGATGTTCATTCCAGTTCTTTTCAAGCTGGTTGTGATTACTGTGCTTGTAATTGCAGAGTCTGCAGATAACTGCATAAAAACGATGTTGCGTAATTGTAAAGTTGCTCGCGTGCTTCCACGAATTGCTGAATGTGCAAAGAATGACCGTAATGCAATACTTCGTGCAAGGTGCTGTGATTATGCACTGTTGATACTAGAGTATTGGGCTGATGCACCTGAAATACAGCGCTCGGCAGATTTGTATGAAGATCTAATAAGGTGTTGCGTGGCCGATGCAATGAGTGAGGTCCGATCAACTGCAAGAATGTGCTATAGAATGTTCTCAAAAACGTGGCCAGAGCGTTCCCGACGGTTATTTTCGCTATTTGATCCTGTTATTCAAAGGTTAATCAATGAAGAGGATGGGGGAATACATAGGCGTCATGCTTCTCCTTCCGTTCGTGATAGGGGTGTGTCACATACTCCGCAGCCATCTGCTGCCTCAAATTTGCCTGGATATGGAACTTCGGCTATTGTTGCAATGGATAGAAGTTCAAGCTTATCATCTGGCAATTCTATCTCATCGGGACTACTTCTGTCGCAAGCTAAGTCTCTTGGTAAAGGTACTGAACGTAGTCTGGAAAGTGTGTTGCATGCAAGCAAACAGAAGGTCAGTGCAATTGAAAGCATGCTTAGAGGTTTGGATTTGTCTGAGAAACATAATTCAACTCTCCAGTCATCAAGCCTGGATCTAGGAGTTGACCCTCCATCATCTCGTGATCCACCATTCCCTGCTGCTGCCCCAGCTTCTAATCATCTCTCAAACTCTTTAATGGCAGATTCAACTACCTCAAGTATCCATAAAAGTAGTAGCCGCAATGGTGGATTGGTTTTGTCCGATATCATTACACAAATTCAAGCTTCCAAAGATTCTGGTAAATCGTCATATCGCAGTAATCTATCAGCAGAAGCTGTGTCAACTGTTTCTTCATACGCAATGAGGAGAGCTTCCGAGAGAACTCAAGAAAGAGGTTCGATCGAAGAGAACAATGACACTAGGGAGGCTAGACGGTTTATGAACTCTCAAATCGATAGGCACTATGACACGTCTCACAGAGATGGGAACTTTAGGGATTCACATAGCAATCATGTGCCGAATTTTCAAAGGCCACTCTTAAGAAAGAATGTGACTGGAAGGATGTCTGCAGGCAGGAGGAGAAGTTTTGATGATAGCCAACTCTCACTCAGGGAAATGTCAAATTATGTAGAAGGTCCCGCATCTCTAAATGATGCTCTGAGTGAGGGACTGAGTCCATCGTCTGACTGGAATGCTAGGGTTGCTGCATTTAATTATCTACGATCTTTGCTGCAGCAAGGCCCAAGGGGAATTCAAGAAGTGATTCAGAATTTTGAGAAAGTAATGAAGTTATTTTTCCAACACTTGGATGATCCCCACCATAAAGTTGCTCAGGCAGCACTTACAACACTAGCAGATATTATTCCATCTTGCCGTAAGCCCTTTGAAAGTTACATGGAAAGGATCCTACCCCATGTTTTCTCACGGTTAATTGACCCAAAGGAGTTAGTTAGGCAGCCGTGTTCGACAACGTTAGATATTGTTAGCAAAACGTACAGTGTAGATTCCCTCTTGCCTGCGTTGCTCCGTTCACTTGATGAACAGCGATCACCAAAGGCCAAACTAGCTGTTATTGAGTTTTCCATTGGTTCTTTAAACAAGCATTCACTGAATCCTGAAGGTTCTGGTAATAGTGGCATCCTTAAATTATGGCTTTCTAAGTTGGCACCATTGGCCCATGAGAAAAATACTAAACTAAAGGAGGCAGCCATTACGTGCATAATATCCGTTTACTCTCACTTTGATTCAGTAGCTGTCTTGAATTTTATTCTTAGTTTGTCAGTTGAAGAACAAAATTCTTTGAGAAGGGCCCTGAAACAGTACACTCCTCGCATTGAAGTGGACCTTATGAACTTCTTGCAAAACAAGAAAGAGCGACAGCGCCTCAAGTCTTATGATCCATCTGATGTAGTTGGAACATCTTCTGAAGAAGGATATGTTAGCGCCTCAAAGAAAAGTCACTTCTTTGGGAGGTATTCTGCTGGTTCAGTTGATAGTGATGGCGGCAGGAAGTGGAGTTCAACCCAAGAATCAGCCATGGTCACCGGCCCTGTTGGCCAAGCAGCTTCTGATGACACACGGGAGAACTTGTATCAGAATTTTGAGACTGGTTCCAACACTGATGTTCTCAATCCAAAATCCAAGGATATGTCTTACACTATGAATTCCATGTGTCAGAACTTCTGCTCCTGGACTAGCCCAGTGGATAAAGTTGATGGTAGAGTAAATTTAGAAAGCTTGTCTACTTCTTGCCTGGACGTTAATGGTCTTATGAGCTTGGACCATATAGGGGTTGCTGAGACTCTTGGGCATGATAGTGAAGCTCCCACCGACTTGGACCCTAATCAATATAAACTTACAGCTGTGAAGGTTAATTCTGCACCAGAATCAGGTCCCAGCATTCCTCAAATACTTCATCTGATCAGCAATGGAAGTGAGGAAAGCCCTACAGCAAGCAAACGTGGTGCACTTCAACAATTAATTGATGCTTCCATAACTAATGACCACTCTCTTTGGACCAAGTACTTCAATCAGATTTTGACTGTTATACTCGAGGTGTTGGATGATTTTGAGTCCTCGATTCGGGAACTCTCTCTTTCCTTGATTGTTGAAATGCTTAAGAACCAGAAAGATGCAATGGAAGATTCTGTTGAGGTCGTAATTGAGAAGCTGCTTCATGTTACGAAGGATGTCGTTCCCAAGGTTTCAAATGAATCCGAGCACTGCTTGAGTATCGTTTTGGCCCAGTATGATCCATTCAGATGTCTAAGCGTTATTGTCCCTCTGTTGGTAACTGAAGATGAGAAAACTCTTGTTACTTGCATCAACTGTTTGACAAAGCTTGTGGGCCGGCTTTCACAAGAGGAATTGATGGCTCAGTTGCCATCATTTTTGCCTGCACTTTTCGAAGCGTTTGGAAACCAGAGTGCTGACGTTCGCAAGACTGTTGTCTTCTGTTTAGTCGATATATACATCATGCTAGGAAAAGCGTTCTTGCCATACCTGGAGGGACTCAACAGCACGCAGTTACGGCTGGTGACTATTTACGCCAACAGAATTTCACAGGCCAGGACGGGCACGTCAATTGACACCAACCATGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000902 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0005876 GO:0005886 GO:0005911 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007026 GO:0007049 GO:0008017 GO:0008092 GO:0008150 GO:0009506 GO:0009524 GO:0009653 GO:0009826 GO:0009987 GO:0010639 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0022607 GO:0030054 GO:0030865 GO:0031023 GO:0031109 GO:0031110 GO:0031111 GO:0031114 GO:0031122 GO:0031647 GO:0032502 GO:0032886 GO:0032989 GO:0033043 GO:0034453 GO:0034622 GO:0040007 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043622 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0046785 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050821 GO:0051010 GO:0051128 GO:0051129 GO:0051258 GO:0051302 GO:0051493 GO:0051494 GO:0051781 GO:0055044 GO:0060560 GO:0065003 GO:0065007 GO:0065008 GO:0070507 GO:0071840 GO:0071944 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1901879 GO:1901880 GO:1902903 GO:1902904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CLASP_N HEAT Vac14_Fab1_bd

Protein Analysis

1439

Amino Acids

159.19

Weight (kDa)

6.6

Isoelectric Point (pI)

47.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CEP104-like_TOG	PF21040	10 - 180	6.9e-10	Centrosomal protein CEP104-like, TOG domain
XMAP215_CLASP_TOG	PF21041	11 - 213	2.4e-09	XMAP215/Dis1/CLASP, TOG domain
HEAT_GCN1_C	PF25786	18 - 116	5.2e-07	GCN1-like, C-terminal HEAT repeats
HEAT	PF02985	160 - 187	7.6e-06	HEAT repeat
CLASP_N	PF12348	280 - 496	1.8e-48	CLASP N terminal
CLASP_N	PF12348	796 - 984	3.9e-06	CLASP N terminal
CEP104-like_TOG	PF21040	798 - 941	5.5e-08	Centrosomal protein CEP104-like, TOG domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001111)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20190
fragaria_vesca	FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_2g19850 FvH4_4g18130
malus_domestica	MD05G1182800.v1.1 MD10G1170900.v1.1
prunus_persica	Prupe.8G209400_v2.0.a1 Prupe.8G209400_v2.0.a1 Prupe.8G209400_v2.0.a1
pyrus_communis	pycom05g16790 pycom10g14730
rosa_chinensis	RchiOBHm_Chr1g0330771 RchiOBHm_Chr1g0330781 RchiOBHm_Chr1g0330801 RchiOBHm_Chr6g0285551
rosa_laevigata	RLG00000012628 RLG00000029789 RLG00000029790 RLG00000029796
rosa_multiflora	Rmu_co8432325.1_g000001 Rmu_sc0000723.1_g000001 Rmu_sc0000723.1_g000002 Rmu_sc0001491.1_g000006 Rmu_sc0002725.1_g000020 Rmu_sc0012495.1_g000004 Rmu_sc0017770.1_g000001 Rmu_sc0031058.1_g000001
rosa_roxburghii	Rroxscaffold_4G00319710 Rroxscaffold_4G00319750 Rroxscaffold_4G00319790 Rroxscaffold_4G00319800 Rroxscaffold_4G00319810 Rroxscaffold_7G00183030
rosa_rugosa	Rorug06G0175400
rosa_samantha	Rh1AG108200 Rh1AG109200 Rh1AG110500 Rh1AG111400 Rh1CG105100 Rh1CG105400 Rh1CG105500 Rh1CG106800 Rh2BG359900 Rh2DG376700 Rh4CG127100 Rh6AG287000 Rh6BG288200 Rh6CG289400 Rh6DG282200
rosa_wichuraiana	Rw0G004360 Rw1G008500 Rw1G008580 Rw1G008750 Rw1G008840 Rw6G024670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	4291
AatII	GACGTC	1 cut(s)	122
AbsI	CCTCGAGG	1 cut(s)	751
AccB1I	GGYRCC	2 cut(s)	600, 2878
AccB7I	CCANNNNNTGG	2 cut(s)	518, 2518
AccBSI	CCGCTC	1 cut(s)	776
AccI	GTMKAC	4 cut(s)	129, 560, 804, 3455
AccII	CGCG	4 cut(s)	23, 25, 297, 1206
AclI	AACGTT	1 cut(s)	2684
AcoI	YGGCCR	3 cut(s)	1365, 1430, 3268
AcuI	CTGAAG	8 cut(s)	148, 452, 578, 644, 992, 2850, 3159, 4029
AcyI	GRCGYC	3 cut(s)	119, 300, 1516
AfaI	GTAC	5 cut(s)	873, 1702, 2708, 3035, 3737
AfeI	AGCGCT	2 cut(s)	12, 1325
AfiI	CCNNNNNNNGG	8 cut(s)	309, 518, 1595, 2518, 3502, 3602, 3795, 4000
AflII	CTTAAG	2 cut(s)	2251, 3830
AflIII	ACRYGT	1 cut(s)	2181
AhdI	GACNNNNNGTC	1 cut(s)	118
AjiI	CACGTC	3 cut(s)	115, 2184, 4293
AjnI	CCWGG	9 cut(s)	174, 377, 517, 1588, 1821, 3401, 3465, 4215, 4280
AloI	GAACNNNNNNTCC	4 cut(s)	3214, 3246, 3383, 3415
Alw21I	GWGCWC	5 cut(s)	21, 213, 998, 3682, 3936
Alw26I	GTCTC	5 cut(s)	175, 1692, 2190, 3311, 3506
Alw44I	GTGCAC	1 cut(s)	3678
AlwNI	CAGNNNCTG	5 cut(s)	79, 1157, 2048, 2966, 3281
Ama87I	CYCGRG	2 cut(s)	751, 3764
Aor51HI	AGCGCT	2 cut(s)	12, 1325
ApaI	GGGCCC	1 cut(s)	3025
ApaLI	GTGCAC	1 cut(s)	3678
ArsI	GACNNNNNNTTYG	4 cut(s)	276, 308, 1731, 1763
AseI	ATTAAT	2 cut(s)	633, 3692
Asp700I	GAANNNNTTC	2 cut(s)	2475, 3133
AsuC2I	CCSGG	1 cut(s)	962
AsuHPI	GGTGA	4 cut(s)	418, 2754, 3247, 4261
AsuII	TTCGAA	1 cut(s)	4119
AvaI	CYCGRG	2 cut(s)	751, 3764
AvaII	GGWCC	9 cut(s)	572, 1017, 1384, 2348, 3055, 3493, 3550, 3605, 3729
BaeGI	GKGCMC	3 cut(s)	3025, 3682, 4292
BaeI	ACNNNNGTAYC	4 cut(s)	855, 888, 1280, 1313
BalI	TGGCCA	2 cut(s)	1432, 3270
BamHI	GGATCC	1 cut(s)	2616
BanI	GGYRCC	2 cut(s)	600, 2878
BanII	GRGCYC	2 cut(s)	21, 3025
BauI	CACGAG	1 cut(s)	1851
BbsI	GAAGAC	1 cut(s)	4156
Bbv12I	GWGCWC	5 cut(s)	21, 213, 998, 3682, 3936
BbvCI	CCTCAGC	1 cut(s)	430
BceAI	ACGGC	4 cut(s)	242, 2571, 2656, 4259
BciT130I	CCWGG	9 cut(s)	176, 379, 519, 1590, 1823, 3403, 3467, 4217, 4282
BciVI	GTATCC	1 cut(s)	1943
BclI	TGATCA	1 cut(s)	3633
BcnI	CCSGG	1 cut(s)	962
BcoDI	GTCTC	5 cut(s)	175, 1692, 2190, 3311, 3506
BfmI	CTRYAG	9 cut(s)	185, 494, 537, 690, 1158, 1408, 2280, 2444, 3660
BfoI	RGCGCY	6 cut(s)	14, 67, 183, 1327, 3097, 3159
BfrI	CTTAAG	2 cut(s)	2251, 3830
BfuI	GTATCC	1 cut(s)	1943
BglI	GCCNNNNNGGC	1 cut(s)	3267
BglII	AGATCT	1 cut(s)	1345
BmcAI	AGTACT	1 cut(s)	3737
Bme18I	GGWCC	9 cut(s)	572, 1017, 1384, 2348, 3055, 3493, 3550, 3605, 3729
BmeRI	GACNNNNNGTC	1 cut(s)	118
BmeT110I	CYCGRG	2 cut(s)	751, 3764
BmgBI	CACGTC	3 cut(s)	115, 2184, 4293
BmiI	GGNNCC	9 cut(s)	314, 602, 2350, 2618, 2880, 3023, 3325, 3552, 3607
BmrI	ACTGGG	2 cut(s)	3407, 3952
BmuI	ACTGGG	2 cut(s)	3407, 3952
BoxI	GACNNNNGTC	1 cut(s)	4160
BpiI	GAAGAC	1 cut(s)	4156
BpmI	CTGGAG	4 cut(s)	35, 415, 1793, 4238
Bpu10I	CCTNAGC	2 cut(s)	430, 2544
Bpu14I	TTCGAA	1 cut(s)	4119
BpuEI	CTTGAG	6 cut(s)	134, 212, 1912, 2082, 3083, 3961
BpuMI	CCSGG	1 cut(s)	962
Bsa29I	ATCGAT	1 cut(s)	2166
BsaAI	YACGTR	1 cut(s)	2928
BsaHI	GRCGYC	3 cut(s)	119, 300, 1516
BsaXI	ACNNNNNCTCC	6 cut(s)	2281, 2311, 3287, 3317, 3383, 3413
Bsc4I	CCNNNNNNNGG	8 cut(s)	309, 518, 1595, 2518, 3502, 3602, 3795, 4000
Bse118I	RCCGGY	2 cut(s)	3257, 4062
Bse1I	ACTGG	8 cut(s)	890, 1109, 1810, 2272, 2405, 3325, 3413, 3958
Bse3DI	GCAATG	8 cut(s)	897, 912, 1380, 1623, 1960, 2079, 3646, 3855
BseBI	CCWGG	9 cut(s)	176, 379, 519, 1590, 1823, 3403, 3467, 4217, 4282
BseCI	ATCGAT	1 cut(s)	2166
BseGI	GGATG	7 cut(s)	535, 1504, 2280, 2527, 2850, 3781, 3907
BseLI	CCNNNNNNNGG	8 cut(s)	309, 518, 1595, 2518, 3502, 3602, 3795, 4000
BseMI	GCAATG	8 cut(s)	897, 912, 1380, 1623, 1960, 2079, 3646, 3855
BseNI	ACTGG	8 cut(s)	890, 1109, 1810, 2272, 2405, 3325, 3413, 3958
BseRI	GAGGAG	4 cut(s)	566, 2092, 2305, 3030
BseSI	GKGCMC	3 cut(s)	3025, 3682, 4292
BseYI	CCCAGC	2 cut(s)	1879, 3608
BsgI	GTGCAG	2 cut(s)	3576, 4095
Bsh1236I	CGCG	4 cut(s)	23, 25, 297, 1206
Bsh1285I	CGRYCG	1 cut(s)	2115
BshNI	GGYRCC	2 cut(s)	600, 2878
BshVI	ATCGAT	1 cut(s)	2166
BsiEI	CGRYCG	1 cut(s)	2115
BsiHKAI	GWGCWC	5 cut(s)	21, 213, 998, 3682, 3936
BsiHKCI	CYCGRG	2 cut(s)	751, 3764
BsiSI	CCGG	4 cut(s)	216, 961, 3258, 4063
BslFI	GGGAC	7 cut(s)	978, 1677, 2334, 2394, 3591, 3977, 4236
BslI	CCNNNNNNNGG	8 cut(s)	309, 518, 1595, 2518, 3502, 3602, 3795, 4000
BsmAI	GTCTC	5 cut(s)	175, 1692, 2190, 3311, 3506
BsmBI	CGTCTC	1 cut(s)	2190
BsmFI	GGGAC	7 cut(s)	978, 1677, 2334, 2394, 3591, 3977, 4236
BsmI	GAATGC	3 cut(s)	2410, 2815, 3612
BsoBI	CYCGRG	2 cut(s)	751, 3764
Bsp119I	TTCGAA	1 cut(s)	4119
Bsp120I	GGGCCC	1 cut(s)	3021
Bsp1286I	GDGCHC	7 cut(s)	21, 213, 998, 3025, 3682, 3936, 4292
Bsp19I	CCATGG	1 cut(s)	3249
BspDI	ATCGAT	1 cut(s)	2166
BspFNI	CGCG	4 cut(s)	23, 25, 297, 1206
BspLI	GGNNCC	9 cut(s)	314, 602, 2350, 2618, 2880, 3023, 3325, 3552, 3607
BspMAI	CTGCAG	4 cut(s)	189, 1162, 2284, 2448
BspT104I	TTCGAA	1 cut(s)	4119
BspT107I	GGYRCC	2 cut(s)	600, 2878
BspTI	CTTAAG	2 cut(s)	2251, 3830
BsrBI	CCGCTC	1 cut(s)	776
BsrDI	GCAATG	8 cut(s)	897, 912, 1380, 1623, 1960, 2079, 3646, 3855
BsrFI	RCCGGY	2 cut(s)	3257, 4062
BsrI	ACTGG	8 cut(s)	890, 1109, 1810, 2272, 2405, 3325, 3413, 3958
BssAI	RCCGGY	2 cut(s)	3257, 4062
BssNAI	GTATAC	1 cut(s)	561
BssNI	GRCGYC	3 cut(s)	119, 300, 1516
BssSI	CACGAG	1 cut(s)	1851
BssT1I	CCWWGG	6 cut(s)	30, 39, 2456, 3249, 3354, 3912
Bst1107I	GTATAC	1 cut(s)	561
Bst2BI	CACGAG	1 cut(s)	1851
Bst2UI	CCWGG	9 cut(s)	176, 379, 519, 1590, 1823, 3403, 3467, 4217, 4282
Bst6I	CTCTTC	3 cut(s)	402, 1488, 2112
BstACI	GRCGYC	3 cut(s)	119, 300, 1516
BstAFI	CTTAAG	2 cut(s)	2251, 3830
BstAPI	GCANNNNNTGC	1 cut(s)	1157
BstBAI	YACGTR	1 cut(s)	2928
BstBI	TTCGAA	1 cut(s)	4119
BstDSI	CCRYGG	1 cut(s)	3249
BstEII	GGTNACC	2 cut(s)	424, 3253
BstF5I	GGATG	7 cut(s)	535, 1504, 2280, 2527, 2850, 3781, 3907
BstFNI	CGCG	4 cut(s)	23, 25, 297, 1206
BstH2I	RGCGCY	6 cut(s)	14, 67, 183, 1327, 3097, 3159
BstMAI	GTCTC	5 cut(s)	175, 1692, 2190, 3311, 3506
BstMCI	CGRYCG	1 cut(s)	2115
BstNI	CCWGG	9 cut(s)	176, 379, 519, 1590, 1823, 3403, 3467, 4217, 4282
BstNSI	RCATGY	3 cut(s)	1092, 1733, 1768
BstPAI	GACNNNNGTC	1 cut(s)	4160
BstPI	GGTNACC	2 cut(s)	424, 3253
BstSFI	CTRYAG	9 cut(s)	185, 494, 537, 690, 1158, 1408, 2280, 2444, 3660
BstSLI	GKGCMC	3 cut(s)	3025, 3682, 4292
BstUI	CGCG	4 cut(s)	23, 25, 297, 1206
BstV2I	GAAGAC	1 cut(s)	4156
BstX2I	RGATCY	5 cut(s)	142, 368, 1345, 1825, 2616
BstXI	CCANNNNNNTGG	1 cut(s)	3547
BstYI	RGATCY	5 cut(s)	142, 368, 1345, 1825, 2616
BstZ17I	GTATAC	1 cut(s)	561
Bsu15I	ATCGAT	1 cut(s)	2166
BsuI	GTATCC	1 cut(s)	1943
BsuTUI	ATCGAT	1 cut(s)	2166
BtgI	CCRYGG	1 cut(s)	3249
BtgZI	GCGATG	1 cut(s)	291
BtrI	CACGTC	3 cut(s)	115, 2184, 4293
BtsCI	GGATG	7 cut(s)	535, 1504, 2280, 2527, 2850, 3781, 3907
BtsI	GCAGTG	2 cut(s)	213, 3934
BtsIMutI	CAGTG	8 cut(s)	213, 1280, 1756, 2716, 2819, 3330, 3420, 3934
CaiI	CAGNNNCTG	5 cut(s)	79, 1157, 2048, 2966, 3281
Cfr10I	RCCGGY	2 cut(s)	3257, 4062
ClaI	ATCGAT	1 cut(s)	2166
CseI	GACGC	3 cut(s)	75, 308, 1505
Csp6I	GTAC	5 cut(s)	872, 1701, 2707, 3034, 3736
CspCI	CAANNNNNGTGG	4 cut(s)	3707, 3742, 4038, 4073
CviQI	GTAC	5 cut(s)	872, 1701, 2707, 3034, 3736
DraI	TTTAAA	1 cut(s)	2808
DrdI	GACNNNNNNGTC	1 cut(s)	4291
DriI	GACNNNNNGTC	1 cut(s)	118
DseDI	GACNNNNNNGTC	1 cut(s)	4291
EaeI	YGGCCR	3 cut(s)	1365, 1430, 3268
Eam1104I	CTCTTC	3 cut(s)	402, 1488, 2112
Eam1105I	GACNNNNNGTC	1 cut(s)	118
EarI	CTCTTC	3 cut(s)	402, 1488, 2112
EciI	GGCGGA	1 cut(s)	190
Ecl136II	GAGCTC	1 cut(s)	19
Eco130I	CCWWGG	6 cut(s)	30, 39, 2456, 3249, 3354, 3912
Eco24I	GRGCYC	2 cut(s)	21, 3025
Eco32I	GATATC	1 cut(s)	1978
Eco47I	GGWCC	9 cut(s)	572, 1017, 1384, 2348, 3055, 3493, 3550, 3605, 3729
Eco47III	AGCGCT	2 cut(s)	12, 1325
Eco53kI	GAGCTC	1 cut(s)	19
Eco57I	CTGAAG	8 cut(s)	148, 452, 578, 644, 992, 2850, 3159, 4029
Eco88I	CYCGRG	2 cut(s)	751, 3764
Eco91I	GGTNACC	2 cut(s)	424, 3253
EcoICRI	GAGCTC	1 cut(s)	19
EcoO109I	RGGNCCY	6 cut(s)	190, 572, 2348, 3021, 3022, 3605
EcoO65I	GGTNACC	2 cut(s)	424, 3253
EcoRI	GAATTC	2 cut(s)	2463, 3376
EcoRII	CCWGG	9 cut(s)	174, 377, 517, 1588, 1821, 3401, 3465, 4215, 4280
EcoRV	GATATC	1 cut(s)	1978
EcoT14I	CCWWGG	6 cut(s)	30, 39, 2456, 3249, 3354, 3912
EcoT38I	GRGCYC	2 cut(s)	21, 3025
ErhI	CCWWGG	6 cut(s)	30, 39, 2456, 3249, 3354, 3912
Esp3I	CGTCTC	1 cut(s)	2190
FalI	AAGNNNNNCTT	2 cut(s)	3042, 3074
FaqI	GGGAC	7 cut(s)	978, 1677, 2334, 2394, 3591, 3977, 4236
FauI	CCCGC	3 cut(s)	46, 262, 2359
FbaI	TGATCA	1 cut(s)	3633
FblI	GTMKAC	4 cut(s)	129, 560, 804, 3455
FokI	GGATG	7 cut(s)	542, 1511, 2287, 2534, 2837, 3788, 3914
FriOI	GRGCYC	2 cut(s)	21, 3025
GsaI	CCCAGC	2 cut(s)	1883, 3612
GsuI	CTGGAG	4 cut(s)	35, 415, 1793, 4238
HaeII	RGCGCY	6 cut(s)	14, 67, 183, 1327, 3097, 3159
HapII	CCGG	4 cut(s)	216, 961, 3258, 4063
HgaI	GACGC	3 cut(s)	75, 308, 1505
Hin1I	GRCGYC	3 cut(s)	119, 300, 1516
HincII	GTYRAC	3 cut(s)	130, 1837, 2055
HindII	GTYRAC	3 cut(s)	130, 1837, 2055
HindIII	AAGCTT	4 cut(s)	1633, 1995, 3448, 4052
HpaII	CCGG	4 cut(s)	216, 961, 3258, 4063
HphI	GGTGA	4 cut(s)	418, 2754, 3247, 4261
Hpy99I	CGWCG	7 cut(s)	112, 124, 131, 263, 302, 1017, 1449
Hsp92I	GRCGYC	3 cut(s)	119, 300, 1516
KroI	GCCGGC	1 cut(s)	4062
KroNI	GCCGGC	1 cut(s)	4064
Ksp22I	TGATCA	1 cut(s)	3633
LmnI	GCTCC	9 cut(s)	16, 43, 61, 237, 318, 773, 2742, 3404, 3541
MbiI	CCGCTC	1 cut(s)	776
MfeI	CAATTG	3 cut(s)	435, 1755, 4296
MflI	RGATCY	5 cut(s)	142, 368, 1345, 1825, 2616
MhlI	GDGCHC	7 cut(s)	21, 213, 998, 3025, 3682, 3936, 4292
MlsI	TGGCCA	2 cut(s)	1432, 3270
MluNI	TGGCCA	2 cut(s)	1432, 3270
MlyI	GAGTC	7 cut(s)	177, 960, 1163, 2395, 3508, 3794, 4218
MmeI	TCCRAC	6 cut(s)	90, 368, 2536, 3106, 3351, 3753
Mox20I	TGGCCA	2 cut(s)	1432, 3270
MroNI	GCCGGC	1 cut(s)	4062
MroXI	GAANNNNTTC	2 cut(s)	2475, 3133
MscI	TGGCCA	2 cut(s)	1432, 3270
MslI	CAYNNNNRTG	6 cut(s)	240, 2193, 2520, 3050, 3528, 3973
Msp20I	TGGCCA	2 cut(s)	1432, 3270
MspA1I	CMGCKG	2 cut(s)	472, 3575
MspCI	CTTAAG	2 cut(s)	2251, 3830
MspI	CCGG	4 cut(s)	216, 961, 3258, 4063
MunI	CAATTG	3 cut(s)	435, 1755, 4296
Mva1269I	GAATGC	3 cut(s)	2410, 2815, 3612
MvaI	CCWGG	9 cut(s)	176, 379, 519, 1590, 1823, 3403, 3467, 4217, 4282
MvnI	CGCG	4 cut(s)	23, 25, 297, 1206
NaeI	GCCGGC	1 cut(s)	4064
NciI	CCSGG	1 cut(s)	962
NcoI	CCATGG	1 cut(s)	3249
NgoMIV	GCCGGC	1 cut(s)	4062
NlaIV	GGNNCC	9 cut(s)	314, 602, 2350, 2618, 2880, 3023, 3325, 3552, 3607
NmeAIII	GCCGAG	1 cut(s)	1307
NmuCI	GTSAC	7 cut(s)	115, 424, 1551, 2263, 3170, 3253, 4249
NspI	RCATGY	3 cut(s)	1092, 1733, 1768
NspV	TTCGAA	1 cut(s)	4119
PaeI	GCATGC	2 cut(s)	1733, 1768
PaeR7I	CTCGAG	2 cut(s)	751, 3764
PasI	CCCWGGG	1 cut(s)	175
PcsI	WCGNNNNNNNCGW	1 cut(s)	116
PctI	GAATGC	3 cut(s)	2410, 2815, 3612
PdiI	GCCGGC	1 cut(s)	4064
PdmI	GAANNNNTTC	2 cut(s)	2475, 3133
PflFI	GACNNNGTC	2 cut(s)	106, 2385
PflMI	CCANNNNNTGG	2 cut(s)	518, 2518
PfoI	TCCNGGA	1 cut(s)	3401
Ple19I	CGATCG	1 cut(s)	2115
PleI	GAGTC	7 cut(s)	176, 960, 1162, 2394, 3508, 3793, 4218
PpsI	GAGTC	7 cut(s)	176, 960, 1162, 2394, 3508, 3793, 4218
Ppu21I	YACGTR	1 cut(s)	2928
PpuMI	RGGWCCY	3 cut(s)	572, 2348, 3605
PshAI	GACNNNNGTC	1 cut(s)	4160
PshBI	ATTAAT	2 cut(s)	633, 3692
Psp124BI	GAGCTC	1 cut(s)	21
Psp1406I	AACGTT	1 cut(s)	2684
Psp5II	RGGWCCY	3 cut(s)	572, 2348, 3605
Psp6I	CCWGG	9 cut(s)	174, 377, 517, 1588, 1821, 3401, 3465, 4215, 4280
PspEI	GGTNACC	2 cut(s)	424, 3253
PspFI	CCCAGC	2 cut(s)	1879, 3608
PspGI	CCWGG	9 cut(s)	174, 377, 517, 1588, 1821, 3401, 3465, 4215, 4280
PspN4I	GGNNCC	9 cut(s)	314, 602, 2350, 2618, 2880, 3023, 3325, 3552, 3607
PspOMI	GGGCCC	1 cut(s)	3021
PspPPI	RGGWCCY	3 cut(s)	572, 2348, 3605
PspXI	VCTCGAGB	2 cut(s)	751, 3764
PstI	CTGCAG	4 cut(s)	189, 1162, 2284, 2448
PstNI	CAGNNNCTG	5 cut(s)	79, 1157, 2048, 2966, 3281
PsuI	RGATCY	5 cut(s)	142, 368, 1345, 1825, 2616
PsyI	GACNNNGTC	2 cut(s)	106, 2385
PvuI	CGATCG	1 cut(s)	2115
PvuII	CAGCTG	1 cut(s)	3575
RsaI	GTAC	5 cut(s)	873, 1702, 2708, 3035, 3737
RsaNI	GTAC	5 cut(s)	872, 1701, 2707, 3034, 3736
RseI	CAYNNNNRTG	6 cut(s)	240, 2193, 2520, 3050, 3528, 3973
SacI	GAGCTC	1 cut(s)	21
SalI	GTCGAC	1 cut(s)	128
ScaI	AGTACT	1 cut(s)	3737
SchI	GAGTC	7 cut(s)	177, 960, 1163, 2395, 3508, 3794, 4218
SduI	GDGCHC	7 cut(s)	21, 213, 998, 3025, 3682, 3936, 4292
SfcI	CTRYAG	9 cut(s)	185, 494, 537, 690, 1158, 1408, 2280, 2444, 3660
SfiI	GGCCNNNNNGGCC	1 cut(s)	3267
Sfr274I	CTCGAG	2 cut(s)	751, 3764
SfuI	TTCGAA	1 cut(s)	4119
SinI	GGWCC	9 cut(s)	572, 1017, 1384, 2348, 3055, 3493, 3550, 3605, 3729
SlaI	CTCGAG	2 cut(s)	751, 3764
SmiMI	CAYNNNNRTG	6 cut(s)	240, 2193, 2520, 3050, 3528, 3973
SphI	GCATGC	2 cut(s)	1733, 1768
SstI	GAGCTC	1 cut(s)	21
StyI	CCWWGG	6 cut(s)	30, 39, 2456, 3249, 3354, 3912
TatI	WGTACW	2 cut(s)	3033, 3735
TauI	GCSGC	4 cut(s)	206, 305, 1953, 3219
TscAI	CASTG	8 cut(s)	213, 1287, 1756, 2716, 2826, 3337, 3420, 3941
TseFI	GTSAC	7 cut(s)	115, 424, 1551, 2263, 3170, 3253, 4249
Tsp45I	GTSAC	7 cut(s)	115, 424, 1551, 2263, 3170, 3253, 4249
TspGWI	ACGGA	5 cut(s)	572, 790, 1522, 2729, 2929
TspRI	CASTG	8 cut(s)	213, 1287, 1756, 2716, 2826, 3337, 3420, 3941
Tth111I	GACNNNGTC	2 cut(s)	106, 2385
Van91I	CCANNNNNTGG	2 cut(s)	518, 2518
Vha464I	CTTAAG	2 cut(s)	2251, 3830
VneI	GTGCAC	1 cut(s)	3678
VpaK11BI	GGWCC	9 cut(s)	572, 1017, 1384, 2348, 3055, 3493, 3550, 3605, 3729
VspI	ATTAAT	2 cut(s)	633, 3692
XceI	RCATGY	3 cut(s)	1092, 1733, 1768
XcmI	CCANNNNNNNNNTGG	3 cut(s)	46, 882, 2397
XhoI	CTCGAG	2 cut(s)	751, 3764
XmiI	GTMKAC	4 cut(s)	129, 560, 804, 3455
XmnI	GAANNNNTTC	2 cut(s)	2475, 3133
ZraI	GACGTC	1 cut(s)	120
ZrmI	AGTACT	1 cut(s)	3737

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.