MD05G1225800.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000423
Physical Location & Seq
Forward (+)
35870713 .. 35872166
1454 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1225800.v1.1.491

Sequence Viewer

Length: 714 bp
ATGGAGCTTAAGCAGCTGTTTGTGATGGCCTCAATTCCGGTGTTGAAAGTGCTCTTGATAACAGCACTTGGCTTATATCTTGCTCTTGATCGTGTCAATATTTTGGGGGAAGTTACTAGAAAGAACTTAAACACTGTTGTGTTTTATGTATTCAGTCCTGCTCTGGTGGGTTCCAACATTGCGCAGACAATTACATATCAAAGCATGGTTAAAATGTGGTTCATGCCCGTGAATATCCTCATCACATTTGTAATTGGTTCCATGCTCGGATGGCTTCTCATCCAATTAACAAGGCCTCCTGCCCATCTGCGAGGCCTTGTACTGGGTTGCTGTGCTGCAGGTAATTTGGGTATGATGCTCCTCGTTATAGTCCCAGCGGTTTGTATAGAAAAAGGGAGCCCATTTGGAGCTTCTGATGTCTGTCACATGTATGCCATGGGTTATGCTTCACTCTCAATGGCGCTATCAGGGATTTCTATCTTTGCTTTTTGTAATGCAATTGTGTTCATGCTTCAGATAGGAGCCGTTTATTTGTGGTCCTATGTGTATAATATTGTGCGGATATCTTCAAGAAGGGGCACCCAAGATTCTAATCAGTCCTCCGAGAGGTCCTCTACATCCGACCAAGTAAGTTGCACTGAGCCTCTGCTATCTTCAAAGGAATGTCATAGTAGAGGAAAAGCAAAGGTTGCTAACTCCATTAAACTACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0044425 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

238

Amino Acids

25.87

Weight (kDa)

9.24

Isoelectric Point (pI)

60.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 11 - 215 1.6e-35 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000423)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76520 AT1G76520 AT1G76530 AT1G76530 AT1G76530
fragaria_vesca FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26370 FvH4_5g26370
malus_domestica MD05G1225800.v1.1 MD06G1002600.v1.1 MD06G1002700.v1.1
prunus_persica Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004300_v2.0.a1
pyrus_communis pycom05g20790 pycom06g00270 pycom06g00280
rosa_chinensis RchiOBHm_Chr7g0217021 RchiOBHm_Chr7g0217081 RchiOBHm_Chr7g0217111 RchiOBHm_Chr7g0217141 RchiOBHm_Chr7g0217181 RchiOBHm_Chr7g0219341
rosa_laevigata RLG00000002504 RLG00000002506 RLG00000002510 RLG00000002512
rosa_multiflora Rmu_sc0001725.1_g000022 Rmu_sc0002460.1_g000002 Rmu_sc0003132.1_g000001 Rmu_sc0007359.1_g000005 Rmu_sc0007359.1_g000009 Rmu_sc0018492.1_g000001 Rmu_sc0039353.1_g000001
rosa_roxburghii Rroxscaffold_3G00242520 Rroxscaffold_3G00242540 Rroxscaffold_3G00242560 Rroxscaffold_3G00242600
rosa_rugosa Rorug07G0165700 Rorug07G0165700 Rorug07G0165800 Rorug07G0165800 Rorug07G0166400 Rorug07G0166500 Rorug07G0166600 Rorug07G0166700 Rorug07G0166800 Rorug07G0166900 Rorug07G0167000 Rorug07G0167000 Rorug07G0167100
rosa_samantha Rh7AG305200 Rh7AG305300 Rh7AG305700 Rh7AG305900 Rh7AG327400 Rh7CG324200 Rh7CG324400 Rh7CG324900 Rh7CG344700 Rh7DG305400 Rh7DG305500 Rh7DG305800 Rh7DG323000
rosa_wichuraiana Rw0G004530 Rw3G011620 Rw7G025930 Rw7G025960 Rw7G025970 Rw7G025990 Rw7G027580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 183
Acc36I ACCTGC 1 cut(s) 329
AccB1I GGYRCC 1 cut(s) 578
AciI CCGC 2 cut(s) 377, 559
AcuI CTGAAG 1 cut(s) 497
AfaI GTAC 1 cut(s) 321
AfiI CCNNNNNNNGG 2 cut(s) 322, 606
AflII CTTAAG 1 cut(s) 8
AflIII ACRYGT 1 cut(s) 426
AgsI TTSAA 3 cut(s) 46, 570, 657
AleI CACNNNNGTG 1 cut(s) 137
AluBI AGCT 3 cut(s) 7, 16, 410
AluI AGCT 3 cut(s) 7, 16, 410
Alw21I GWGCWC 1 cut(s) 54
AoxI GGCC 3 cut(s) 27, 293, 313
ApeKI GCWGC 2 cut(s) 13, 335
AspLEI GCGC 2 cut(s) 184, 463
AspS9I GGNCC 2 cut(s) 537, 609
AvaII GGWCC 2 cut(s) 537, 609
BaeGI GKGCMC 1 cut(s) 581
BanI GGYRCC 1 cut(s) 578
BanII GRGCYC 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 2 cut(s) 25, 322
BccI CCATC 3 cut(s) 19, 264, 312
BceAI ACGGC 1 cut(s) 509
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 1 cut(s) 336
BfoI RGCGCY 1 cut(s) 464
BfrI CTTAAG 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 329
BisI GCNGC 2 cut(s) 14, 336
BlsI GCNGC 2 cut(s) 15, 337
Bme18I GGWCC 2 cut(s) 537, 609
BmgT120I GGNCC 2 cut(s) 537, 609
BmiI GGNNCC 5 cut(s) 172, 259, 398, 523, 580
BmrI ACTGGG 1 cut(s) 332
BmsI GCATC 1 cut(s) 345
BmuI ACTGGG 1 cut(s) 332
BplI GAGNNNNNCTC 2 cut(s) 596, 628
BsaBI GATNNNNATC 2 cut(s) 476, 591
BsaJI CCNNGG 1 cut(s) 435
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 606
Bse1I ACTGG 1 cut(s) 327
Bse3DI GCAATG 1 cut(s) 177
Bse8I GATNNNNATC 2 cut(s) 476, 591
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 3 cut(s) 275, 279, 617
BseJI GATNNNNATC 2 cut(s) 476, 591
BseLI CCNNNNNNNGG 2 cut(s) 322, 606
BseMI GCAATG 1 cut(s) 177
BseMII CTCAG 1 cut(s) 630
BseNI ACTGG 1 cut(s) 327
BseRI GAGGAG 1 cut(s) 350
BseSI GKGCMC 1 cut(s) 581
BseXI GCAGC 2 cut(s) 25, 322
BseYI CCCAGC 1 cut(s) 373
BshFI GGCC 3 cut(s) 29, 295, 315
BshNI GGYRCC 1 cut(s) 578
BsiHKAI GWGCWC 1 cut(s) 54
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 2 cut(s) 322, 606
BsmFI GGGAC 1 cut(s) 356
BsnI GGCC 3 cut(s) 29, 295, 315
Bsp1286I GDGCHC 3 cut(s) 54, 401, 581
Bsp143I GATC 1 cut(s) 88
Bsp19I CCATGG 1 cut(s) 435
BspACI CCGC 2 cut(s) 377, 559
BspANI GGCC 3 cut(s) 29, 295, 315
BspCNI CTCAG 1 cut(s) 631
BspLI GGNNCC 5 cut(s) 172, 259, 398, 523, 580
BspMAI CTGCAG 1 cut(s) 340
BspMI ACCTGC 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 578
BspTI CTTAAG 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 177
BsrI ACTGG 1 cut(s) 327
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 1 cut(s) 88
BssT1I CCWWGG 1 cut(s) 435
Bst4CI ACNGT 1 cut(s) 136
BstAFI CTTAAG 1 cut(s) 8
BstAPI GCANNNNNTGC 1 cut(s) 689
BstDEI CTNAG 1 cut(s) 639
BstDSI CCRYGG 1 cut(s) 435
BstENI CCTNNNNNAGG 1 cut(s) 604
BstF5I GGATG 3 cut(s) 275, 279, 617
BstH2I RGCGCY 1 cut(s) 464
BstHHI GCGC 2 cut(s) 184, 463
BstKTI GATC 1 cut(s) 91
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 3 cut(s) 13, 271, 689
BstNSI RCATGY 1 cut(s) 430
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 581
BstV1I GCAGC 2 cut(s) 25, 322
BsuRI GGCC 3 cut(s) 29, 295, 315
BtgI CCRYGG 1 cut(s) 435
BtsCI GGATG 3 cut(s) 275, 279, 617
BtsIMutI CAGTG 2 cut(s) 132, 636
BveI ACCTGC 1 cut(s) 329
CfoI GCGC 2 cut(s) 184, 463
Cfr13I GGNCC 2 cut(s) 537, 609
Csp6I GTAC 1 cut(s) 320
CviAII CATG 6 cut(s) 205, 223, 262, 427, 436, 508
CviQI GTAC 1 cut(s) 320
DdeI CTNAG 1 cut(s) 639
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 435
Eco147I AGGCCT 2 cut(s) 295, 315
Eco24I GRGCYC 1 cut(s) 401
Eco32I GATATC 1 cut(s) 564
Eco47I GGWCC 2 cut(s) 537, 609
Eco57I CTGAAG 1 cut(s) 497
EcoNI CCTNNNNNAGG 1 cut(s) 604
EcoO109I RGGNCCY 1 cut(s) 609
EcoRV GATATC 1 cut(s) 564
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 401
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 6 cut(s) 208, 226, 265, 430, 439, 511
FaqI GGGAC 1 cut(s) 356
FatI CATG 6 cut(s) 204, 222, 261, 426, 435, 507
Fnu4HI GCNGC 2 cut(s) 14, 336
FokI GGATG 3 cut(s) 266, 282, 604
FriOI GRGCYC 1 cut(s) 401
Fsp4HI GCNGC 2 cut(s) 14, 336
FspBI CTAG 1 cut(s) 117
FspI TGCGCA 1 cut(s) 183
GlaI GCGC 2 cut(s) 183, 462
GluI GCNGC 2 cut(s) 14, 336
GsaI CCCAGC 1 cut(s) 377
HaeII RGCGCY 1 cut(s) 464
HaeIII GGCC 3 cut(s) 29, 295, 315
HapII CCGG 1 cut(s) 38
HhaI GCGC 2 cut(s) 184, 463
Hin1II CATG 6 cut(s) 208, 226, 265, 430, 439, 511
Hin6I GCGC 2 cut(s) 182, 461
HinP1I GCGC 2 cut(s) 182, 461
HinfI GANTC 1 cut(s) 587
HpaII CCGG 1 cut(s) 38
Hpy188I TCNGA 5 cut(s) 269, 415, 516, 604, 622
Hpy188III TCNNGA 3 cut(s) 55, 86, 570
HpyAV CCTTC 1 cut(s) 567
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4V TGCA 3 cut(s) 338, 497, 636
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 271, 689
HpyF3I CTNAG 1 cut(s) 639
Hsp92II CATG 6 cut(s) 208, 226, 265, 430, 439, 511
HspAI GCGC 2 cut(s) 182, 461
Kzo9I GATC 1 cut(s) 88
LmnI GCTCC 5 cut(s) 4, 363, 396, 407, 521
LpnPI CCDG 8 cut(s) 51, 149, 171, 308, 312, 324, 387, 453
Lsp1109I GCAGC 2 cut(s) 25, 322
LweI GCATC 1 cut(s) 345
MaeI CTAG 1 cut(s) 117
MaeIII GTNAC 2 cut(s) 112, 422
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MboII GAAGA 2 cut(s) 558, 645
MfeI CAATTG 1 cut(s) 498
MhlI GDGCHC 3 cut(s) 54, 401, 581
MluCI AATT 6 cut(s) 33, 189, 252, 284, 343, 498
MmeI TCCRAC 2 cut(s) 198, 645
MseI TTAA 5 cut(s) 9, 128, 210, 287, 702
MslI CAYNNNNRTG 3 cut(s) 137, 227, 429
MspA1I CMGCKG 2 cut(s) 16, 377
MspCI CTTAAG 1 cut(s) 8
MspI CCGG 1 cut(s) 38
MunI CAATTG 1 cut(s) 498
MwoI GCNNNNNNNGC 3 cut(s) 13, 271, 689
NcoI CCATGG 1 cut(s) 435
NdeII GATC 1 cut(s) 88
NlaIII CATG 6 cut(s) 208, 226, 265, 430, 439, 511
NlaIV GGNNCC 5 cut(s) 172, 259, 398, 523, 580
NmuCI GTSAC 1 cut(s) 422
NsbI TGCGCA 1 cut(s) 183
NspI RCATGY 1 cut(s) 430
OliI CACNNNNGTG 1 cut(s) 137
PceI AGGCCT 2 cut(s) 295, 315
PciI ACATGT 1 cut(s) 426
PfeI GAWTC 1 cut(s) 587
PkrI GCNGC 2 cut(s) 15, 337
PpuMI RGGWCCY 1 cut(s) 609
PscI ACATGT 1 cut(s) 426
Psp5II RGGWCCY 1 cut(s) 609
PspFI CCCAGC 1 cut(s) 373
PspN4I GGNNCC 5 cut(s) 172, 259, 398, 523, 580
PspPI GGNCC 2 cut(s) 537, 609
PspPPI RGGWCCY 1 cut(s) 609
PstI CTGCAG 1 cut(s) 340
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 1 cut(s) 321
RsaNI GTAC 1 cut(s) 320
RseI CAYNNNNRTG 3 cut(s) 137, 227, 429
SaqAI TTAA 5 cut(s) 9, 128, 210, 287, 702
SatI GCNGC 2 cut(s) 14, 336
Sau3AI GATC 1 cut(s) 88
Sau96I GGNCC 2 cut(s) 537, 609
SduI GDGCHC 3 cut(s) 54, 401, 581
SetI ASST 6 cut(s) 9, 18, 343, 412, 611, 690
SfaNI GCATC 1 cut(s) 345
SfcI CTRYAG 1 cut(s) 336
SinI GGWCC 2 cut(s) 537, 609
SmiMI CAYNNNNRTG 3 cut(s) 137, 227, 429
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 6 cut(s) 33, 189, 252, 284, 343, 498
SseBI AGGCCT 2 cut(s) 295, 315
SsiI CCGC 2 cut(s) 377, 559
SspI AATATT 2 cut(s) 100, 553
SspMI CTAG 1 cut(s) 117
StuI AGGCCT 2 cut(s) 295, 315
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 1 cut(s) 136
TasI AATT 6 cut(s) 33, 189, 252, 284, 343, 498
TatI WGTACW 1 cut(s) 319
TfiI GAWTC 1 cut(s) 587
Tru1I TTAA 5 cut(s) 9, 128, 210, 287, 702
Tru9I TTAA 5 cut(s) 9, 128, 210, 287, 702
TscAI CASTG 2 cut(s) 139, 643
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 2 cut(s) 13, 335
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 2 cut(s) 211, 496
TspRI CASTG 2 cut(s) 139, 643
Vha464I CTTAAG 1 cut(s) 8
VpaK11BI GGWCC 2 cut(s) 537, 609
XagI CCTNNNNNAGG 1 cut(s) 604
XceI RCATGY 1 cut(s) 430
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.