Rw7G025960

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000423
Physical Location & Seq
Forward (+)
32813705 .. 32816240
2536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025960.1

Sequence Viewer

Length: 1212 bp
ATGGCGCTTTTGCAGCTCTTCATCACCGCATCGATTCCGGTCTTGAAAGTGCTCTTGATCACTGCACTTGGCTCCTATCTTGCACTTGGGCGCGTTAATATTCTGGGGGAAGATACCAGGAAGAACTTGAACACTGTTGTATTTTATGTATTCAGTCCAGCTCTGGTGGTAACTAACCTTGCTCAGACTATTACATATGACAGTTTGATTAAACTGTGGTTTATGCCATTGAACATCTTAATCACCTTCATTGTTGGTTCTGTACTTGGATGGATACTCATCCAATTAGCAAGACCACCTCCACATCTCCGAGGCCTCATTTTGGGTTGCTGTGCGGCTGGAAATTTGGGGAACTTGCTCCTCATTATAGTCCCAGCAGTCTGTAAAGAGAAGGGAAGCCCATTTGGAGCTCCTGATGTCTGTCACACTTATGGAATGGCTTATGCATCACTTTCAATGGCAACAGGAGTCATTTATATTTGGTCCTATGTGTATAACATTGTGAGGATATCTTCAAAGAGGAGTACCCAAGACTCCCCCCAGTCTTTGGAGGAACCCTCCGAATCAAACCAAGTAAGTTGCACCGAGCCTCTGCTTTCTTCCAAGGAATATGGGATAGTAGAGGACAATGCCGATCAGTATGCACTGCCCTGCACTATATCTCAACAAGACTCTAAGATAGAACCTCCGAGTAAATCCAAGAACTGTCTAGTGAGACTGTTTCAAAAGCTTAATCTGAAAACGATATTTGCCCCTTCAACTATTGGAGCGATTCGTAAACTAATAATAGGAGATGGTGCTCCTCTACGGGTGATCCAAGACACTACTAAGTTGTTGGGAGATGGAGCTATCCCCGCTCTCACTTTGATAATTGGAGGAAACCTCCTTAAAGGTTTGAGAGAGTCAGGGATACAGAAATCTATTGTTGTTGGGATCATATTCGTCCGTTACGTGTTCCTGCCTCTTATAGGCATATTGATCATTAAAGGGGCGCTAAAATTTGGCTTAGTGCACTCTGATCCATTATATCTATTTGTTCTCCTGATTCAGTATACACTACCACCTGCAATGAACATAGGAACGATCACCCAATTGTTCGGAGCGGGAGAGAACGAATGTTCAGTAATACTGCTGTGGACTTACGCATTTGCATCAGTATCACTTACTTTATGGTCTGCCTTCTTCATGTGGTTTGTGGCCAGGCAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0044425 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

403

Amino Acids

43.95

Weight (kDa)

8.91

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 10 - 391 2.2e-76 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000423)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76520 AT1G76520 AT1G76530 AT1G76530 AT1G76530
fragaria_vesca FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26370 FvH4_5g26370
malus_domestica MD05G1225800.v1.1 MD06G1002600.v1.1 MD06G1002700.v1.1
prunus_persica Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004300_v2.0.a1
pyrus_communis pycom05g20790 pycom06g00270 pycom06g00280
rosa_chinensis RchiOBHm_Chr7g0217021 RchiOBHm_Chr7g0217081 RchiOBHm_Chr7g0217111 RchiOBHm_Chr7g0217141 RchiOBHm_Chr7g0217181 RchiOBHm_Chr7g0219341
rosa_laevigata RLG00000002504 RLG00000002506 RLG00000002510 RLG00000002512
rosa_multiflora Rmu_sc0001725.1_g000022 Rmu_sc0002460.1_g000002 Rmu_sc0003132.1_g000001 Rmu_sc0007359.1_g000005 Rmu_sc0007359.1_g000009 Rmu_sc0018492.1_g000001 Rmu_sc0039353.1_g000001
rosa_roxburghii Rroxscaffold_3G00242520 Rroxscaffold_3G00242540 Rroxscaffold_3G00242560 Rroxscaffold_3G00242600
rosa_rugosa Rorug07G0165700 Rorug07G0165700 Rorug07G0165800 Rorug07G0165800 Rorug07G0166400 Rorug07G0166500 Rorug07G0166600 Rorug07G0166700 Rorug07G0166800 Rorug07G0166900 Rorug07G0167000 Rorug07G0167000 Rorug07G0167100
rosa_samantha Rh7AG305200 Rh7AG305300 Rh7AG305700 Rh7AG305900 Rh7AG327400 Rh7CG324200 Rh7CG324400 Rh7CG324900 Rh7CG344700 Rh7DG305400 Rh7DG305500 Rh7DG305800 Rh7DG323000
rosa_wichuraiana Rw0G004530 Rw3G011620 Rw7G025930 Rw7G025960 Rw7G025970 Rw7G025990 Rw7G027580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1072
Acc36I ACCTGC 1 cut(s) 1072
AccB7I CCANNNNNTGG 1 cut(s) 547
AccBSI CCGCTC 2 cut(s) 857, 1103
AccI GTMKAC 1 cut(s) 1052
AccII CGCG 1 cut(s) 93
AciI CCGC 4 cut(s) 27, 335, 855, 1103
AclWI GGATC 3 cut(s) 808, 941, 1013
AcoI YGGCCR 1 cut(s) 1197
AcsI RAATTY 2 cut(s) 343, 998
AfaI GTAC 2 cut(s) 264, 526
AfiI CCNNNNNNNGG 3 cut(s) 322, 547, 968
AflIII ACRYGT 1 cut(s) 951
AgsI TTSAA 7 cut(s) 46, 130, 232, 456, 516, 725, 759
AjnI CCWGG 2 cut(s) 116, 1199
AluBI AGCT 5 cut(s) 16, 161, 410, 730, 848
AluI AGCT 5 cut(s) 16, 161, 410, 730, 848
Alw21I GWGCWC 4 cut(s) 54, 412, 802, 1014
Alw26I GTCTC 1 cut(s) 709
Alw44I GTGCAC 1 cut(s) 1010
AlwI GGATC 3 cut(s) 808, 941, 1013
AoxI GGCC 2 cut(s) 313, 1197
ApaLI GTGCAC 1 cut(s) 1010
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 2 cut(s) 343, 998
AspLEI GCGC 3 cut(s) 7, 93, 994
AspS9I GGNCC 1 cut(s) 483
AsuHPI GGTGA 4 cut(s) 16, 235, 823, 1078
AvaII GGWCC 1 cut(s) 483
BaeGI GKGCMC 1 cut(s) 1014
BalI TGGCCA 1 cut(s) 1199
BanII GRGCYC 1 cut(s) 412
Bbv12I GWGCWC 4 cut(s) 54, 412, 802, 1014
BbvI GCAGC 1 cut(s) 25
BccI CCATC 3 cut(s) 264, 788, 836
BcgI CGANNNNNNTGC 2 cut(s) 623, 657
BciT130I CCWGG 2 cut(s) 118, 1201
BciVI GTATCC 2 cut(s) 267, 903
BclI TGATCA 2 cut(s) 57, 978
BcoDI GTCTC 1 cut(s) 709
BfaI CTAG 1 cut(s) 710
BfoI RGCGCY 2 cut(s) 8, 995
BfuAI ACCTGC 1 cut(s) 1072
BfuI GTATCC 2 cut(s) 267, 903
BisI GCNGC 2 cut(s) 14, 336
BlsI GCNGC 2 cut(s) 15, 337
Bme1390I CCNGG 2 cut(s) 118, 1201
Bme18I GGWCC 1 cut(s) 483
BmgT120I GGNCC 1 cut(s) 483
BmiI GGNNCC 2 cut(s) 73, 555
BmrFI CCNGG 2 cut(s) 118, 1201
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 3 cut(s) 38, 455, 1160
BmuI ACTGGG 1 cut(s) 535
BplI GAGNNNNNCTC 4 cut(s) 542, 574, 867, 899
Bsa29I ATCGAT 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 952
BsaBI GATNNNNATC 1 cut(s) 278
BsaJI CCNNGG 2 cut(s) 310, 603
BsaWI WCCGGW 1 cut(s) 37
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 547, 968
Bse1I ACTGG 1 cut(s) 541
Bse3DI GCAATG 1 cut(s) 1074
Bse8I GATNNNNATC 1 cut(s) 278
BseBI CCWGG 2 cut(s) 118, 1201
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 310, 603
BseGI GGATG 2 cut(s) 275, 279
BseJI GATNNNNATC 1 cut(s) 278
BseLI CCNNNNNNNGG 3 cut(s) 322, 547, 968
BseMI GCAATG 1 cut(s) 1074
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 1 cut(s) 541
BseRI GAGGAG 3 cut(s) 350, 535, 792
BseSI GKGCMC 1 cut(s) 1014
BseXI GCAGC 1 cut(s) 25
BseYI CCCAGC 1 cut(s) 373
BsgI GTGCAG 2 cut(s) 48, 637
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 315, 1199
BshVI ATCGAT 1 cut(s) 32
BsiHKAI GWGCWC 4 cut(s) 54, 412, 802, 1014
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 3 cut(s) 322, 547, 968
BsmAI GTCTC 1 cut(s) 709
BsmFI GGGAC 1 cut(s) 356
BsnI GGCC 2 cut(s) 315, 1199
Bsp1286I GDGCHC 4 cut(s) 54, 412, 802, 1014
Bsp143I GATC 7 cut(s) 57, 634, 813, 933, 978, 1018, 1083
BspACI CCGC 4 cut(s) 27, 335, 855, 1103
BspANI GGCC 2 cut(s) 315, 1199
BspCNI CTCAG 1 cut(s) 196
BspDI ATCGAT 1 cut(s) 32
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 73, 555
BspMI ACCTGC 1 cut(s) 1072
BspPI GGATC 3 cut(s) 808, 941, 1013
BspQI GCTCTTC 1 cut(s) 23
BsrBI CCGCTC 2 cut(s) 857, 1103
BsrDI GCAATG 1 cut(s) 1074
BsrI ACTGG 1 cut(s) 541
BssECI CCNNGG 2 cut(s) 310, 603
BssMI GATC 7 cut(s) 57, 634, 813, 933, 978, 1018, 1083
BssNAI GTATAC 1 cut(s) 1053
BssT1I CCWWGG 1 cut(s) 603
Bst1107I GTATAC 1 cut(s) 1053
Bst2UI CCWGG 2 cut(s) 118, 1201
Bst4CI ACNGT 5 cut(s) 136, 203, 216, 707, 720
Bst6I CTCTTC 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 952
BstDEI CTNAG 4 cut(s) 183, 675, 828, 1006
BstENI CCTNNNNNAGG 1 cut(s) 966
BstF5I GGATG 2 cut(s) 275, 279
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 2 cut(s) 8, 995
BstHHI GCGC 3 cut(s) 7, 93, 994
BstKTI GATC 7 cut(s) 60, 637, 816, 936, 981, 1021, 1086
BstMAI GTCTC 1 cut(s) 709
BstMBI GATC 7 cut(s) 57, 634, 813, 933, 978, 1018, 1083
BstMWI GCNNNNNNNGC 2 cut(s) 13, 854
BstNI CCWGG 2 cut(s) 118, 1201
BstSCI CCNGG 2 cut(s) 116, 1199
BstSLI GKGCMC 1 cut(s) 1014
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 25
BstZ17I GTATAC 1 cut(s) 1053
Bsu15I ATCGAT 1 cut(s) 32
BsuI GTATCC 2 cut(s) 267, 903
BsuRI GGCC 2 cut(s) 315, 1199
BsuTUI ATCGAT 1 cut(s) 32
BtsCI GGATG 2 cut(s) 275, 279
BtsI GCAGTG 2 cut(s) 60, 644
BtsIMutI CAGTG 3 cut(s) 60, 132, 644
BveI ACCTGC 1 cut(s) 1072
CfoI GCGC 3 cut(s) 7, 93, 994
Cfr13I GGNCC 1 cut(s) 483
ClaI ATCGAT 1 cut(s) 32
Csp6I GTAC 2 cut(s) 263, 525
CviAII CATG 1 cut(s) 1186
CviQI GTAC 2 cut(s) 263, 525
DdeI CTNAG 4 cut(s) 183, 675, 828, 1006
DpnI GATC 7 cut(s) 59, 636, 815, 935, 980, 1020, 1085
DpnII GATC 7 cut(s) 57, 634, 813, 933, 978, 1018, 1083
EaeI YGGCCR 1 cut(s) 1197
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 603
Eco147I AGGCCT 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 412
Eco32I GATATC 1 cut(s) 510
Eco47I GGWCC 1 cut(s) 483
Eco53kI GAGCTC 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 410
EcoNI CCTNNNNNAGG 1 cut(s) 966
EcoRII CCWGG 2 cut(s) 116, 1199
EcoRV GATATC 1 cut(s) 510
EcoT14I CCWWGG 1 cut(s) 603
EcoT22I ATGCAT 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 1 cut(s) 1189
FaqI GGGAC 1 cut(s) 356
FatI CATG 1 cut(s) 1185
FauI CCCGC 2 cut(s) 862, 1096
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 2 cut(s) 57, 978
FblI GTMKAC 1 cut(s) 1052
Fnu4HI GCNGC 2 cut(s) 14, 336
FokI GGATG 2 cut(s) 266, 282
FriOI GRGCYC 1 cut(s) 412
Fsp4HI GCNGC 2 cut(s) 14, 336
FspBI CTAG 1 cut(s) 710
GlaI GCGC 3 cut(s) 6, 92, 993
GluI GCNGC 2 cut(s) 14, 336
GsaI CCCAGC 1 cut(s) 377
HaeII RGCGCY 2 cut(s) 8, 995
HaeIII GGCC 2 cut(s) 315, 1199
HapII CCGG 1 cut(s) 38
HhaI GCGC 3 cut(s) 7, 93, 994
Hin1II CATG 1 cut(s) 1189
Hin6I GCGC 3 cut(s) 5, 91, 992
HinP1I GCGC 3 cut(s) 5, 91, 992
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 8 cut(s) 34, 468, 533, 563, 671, 772, 902, 1045
HpaII CCGG 1 cut(s) 38
HphI GGTGA 4 cut(s) 16, 235, 823, 1078
Hpy166II GTNNAC 4 cut(s) 779, 1012, 1053, 1137
Hpy188I TCNGA 7 cut(s) 186, 311, 562, 690, 738, 1018, 1100
Hpy188III TCNNGA 4 cut(s) 43, 55, 413, 1042
Hpy8I GTNNAC 4 cut(s) 779, 1012, 1053, 1137
HpyAV CCTTC 4 cut(s) 256, 385, 765, 1189
HpyCH4III ACNGT 5 cut(s) 136, 203, 216, 707, 720
HpyCH4IV ACGT 1 cut(s) 951
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 854
HpyF3I CTNAG 4 cut(s) 183, 675, 828, 1006
HpySE526I ACGT 1 cut(s) 951
Hsp92II CATG 1 cut(s) 1189
HspAI GCGC 3 cut(s) 5, 91, 992
Ksp22I TGATCA 2 cut(s) 57, 978
Kzo9I GATC 7 cut(s) 57, 634, 813, 933, 978, 1018, 1083
LguI GCTCTTC 1 cut(s) 23
LmnI GCTCC 8 cut(s) 77, 363, 407, 415, 767, 805, 845, 1100
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 3 cut(s) 38, 455, 1160
MaeI CTAG 1 cut(s) 710
MaeII ACGT 1 cut(s) 951
MaeIII GTNAC 3 cut(s) 169, 422, 947
MalI GATC 7 cut(s) 59, 636, 815, 935, 980, 1020, 1085
MbiI CCGCTC 2 cut(s) 857, 1103
MboI GATC 7 cut(s) 57, 634, 813, 933, 978, 1018, 1083
MboII GAAGA 6 cut(s) 10, 122, 133, 504, 591, 1174
MfeI CAATTG 1 cut(s) 1091
MhlI GDGCHC 4 cut(s) 54, 412, 802, 1014
MlsI TGGCCA 1 cut(s) 1199
MluCI AATT 5 cut(s) 284, 343, 870, 998, 1091
MluNI TGGCCA 1 cut(s) 1199
MlyI GAGTC 4 cut(s) 477, 527, 665, 911
Mox20I TGGCCA 1 cut(s) 1199
Mph1103I ATGCAT 1 cut(s) 448
MscI TGGCCA 1 cut(s) 1199
MseI TTAA 6 cut(s) 96, 210, 239, 732, 888, 984
MslI CAYNNNNRTG 1 cut(s) 429
Msp20I TGGCCA 1 cut(s) 1199
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 2 cut(s) 118, 1201
MunI CAATTG 1 cut(s) 1091
MvaI CCWGG 2 cut(s) 118, 1201
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 2 cut(s) 13, 854
NdeI CATATG 1 cut(s) 196
NdeII GATC 7 cut(s) 57, 634, 813, 933, 978, 1018, 1083
NlaIII CATG 1 cut(s) 1189
NlaIV GGNNCC 2 cut(s) 73, 555
NmuCI GTSAC 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 448
PaqCI CACCTGC 1 cut(s) 1072
PceI AGGCCT 1 cut(s) 315
PciSI GCTCTTC 1 cut(s) 23
PcsI WCGNNNNNNNCGW 1 cut(s) 948
PfeI GAWTC 4 cut(s) 34, 563, 772, 1045
PflMI CCANNNNNTGG 1 cut(s) 547
PkrI GCNGC 2 cut(s) 15, 337
PleI GAGTC 4 cut(s) 476, 527, 665, 910
PpsI GAGTC 4 cut(s) 476, 527, 665, 910
Ppu21I YACGTR 1 cut(s) 952
Psp124BI GAGCTC 1 cut(s) 412
Psp6I CCWGG 2 cut(s) 116, 1199
PspFI CCCAGC 1 cut(s) 373
PspGI CCWGG 2 cut(s) 116, 1199
PspN4I GGNNCC 2 cut(s) 73, 555
PspPI GGNCC 1 cut(s) 483
RsaI GTAC 2 cut(s) 264, 526
RsaNI GTAC 2 cut(s) 263, 525
RseI CAYNNNNRTG 1 cut(s) 429
SacI GAGCTC 1 cut(s) 412
SapI GCTCTTC 1 cut(s) 23
SaqAI TTAA 6 cut(s) 96, 210, 239, 732, 888, 984
SatI GCNGC 2 cut(s) 14, 336
Sau3AI GATC 7 cut(s) 57, 634, 813, 933, 978, 1018, 1083
Sau96I GGNCC 1 cut(s) 483
SchI GAGTC 4 cut(s) 477, 527, 665, 911
ScrFI CCNGG 2 cut(s) 118, 1201
SduI GDGCHC 4 cut(s) 54, 412, 802, 1014
SfaNI GCATC 3 cut(s) 38, 455, 1160
SinI GGWCC 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 429
Sse9I AATT 5 cut(s) 284, 343, 870, 998, 1091
SseBI AGGCCT 1 cut(s) 315
SsiI CCGC 4 cut(s) 27, 335, 855, 1103
SspI AATATT 1 cut(s) 100
SspMI CTAG 1 cut(s) 710
SstI GAGCTC 1 cut(s) 412
StuI AGGCCT 1 cut(s) 315
StyD4I CCNGG 2 cut(s) 116, 1199
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 5 cut(s) 136, 203, 216, 707, 720
TaiI ACGT 1 cut(s) 954
TaqI TCGA 1 cut(s) 32
TasI AATT 5 cut(s) 284, 343, 870, 998, 1091
TatI WGTACW 1 cut(s) 262
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 4 cut(s) 34, 563, 772, 1045
Tru1I TTAA 6 cut(s) 96, 210, 239, 732, 888, 984
Tru9I TTAA 6 cut(s) 96, 210, 239, 732, 888, 984
TscAI CASTG 3 cut(s) 67, 139, 651
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 4 cut(s) 10, 238, 1085, 1174
TspGWI ACGGA 1 cut(s) 935
TspRI CASTG 3 cut(s) 67, 139, 651
Van91I CCANNNNNTGG 1 cut(s) 547
VneI GTGCAC 1 cut(s) 1010
VpaK11BI GGWCC 1 cut(s) 483
XagI CCTNNNNNAGG 1 cut(s) 966
XapI RAATTY 2 cut(s) 343, 998
XmiI GTMKAC 1 cut(s) 1052
XspI CTAG 1 cut(s) 710
Zsp2I ATGCAT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.