Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 368 |
| AarI | CACCTGC | 1 cut(s) | 1153 |
| Acc36I | ACCTGC | 1 cut(s) | 1153 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 547 |
| AccBSI | CCGCTC | 2 cut(s) | 938, 1352 |
| AccI | GTMKAC | 1 cut(s) | 1133 |
| AccII | CGCG | 1 cut(s) | 93 |
| AciI | CCGC | 5 cut(s) | 27, 335, 623, 936, 1352 |
| AclI | AACGTT | 1 cut(s) | 177 |
| AclWI | GGATC | 3 cut(s) | 889, 1022, 1094 |
| AcsI | RAATTY | 3 cut(s) | 343, 774, 1079 |
| AcuI | CTGAAG | 1 cut(s) | 1466 |
| AfaI | GTAC | 2 cut(s) | 264, 526 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 547, 1049, 1270 |
| AflIII | ACRYGT | 1 cut(s) | 1032 |
| AgsI | TTSAA | 7 cut(s) | 46, 130, 456, 516, 773, 807, 1220 |
| AjnI | CCWGG | 2 cut(s) | 116, 985 |
| AluBI | AGCT | 7 cut(s) | 7, 16, 161, 410, 679, 929, 1333 |
| AluI | AGCT | 7 cut(s) | 7, 16, 161, 410, 679, 929, 1333 |
| Alw21I | GWGCWC | 2 cut(s) | 412, 1095 |
| Alw26I | GTCTC | 1 cut(s) | 1244 |
| Alw44I | GTGCAC | 1 cut(s) | 1091 |
| AlwI | GGATC | 3 cut(s) | 889, 1022, 1094 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1283 |
| AoxI | GGCC | 4 cut(s) | 983, 1084, 1250, 1474 |
| ApaLI | GTGCAC | 1 cut(s) | 1091 |
| ApeKI | GCWGC | 1 cut(s) | 13 |
| ApoI | RAATTY | 3 cut(s) | 343, 774, 1079 |
| AseI | ATTAAT | 1 cut(s) | 696 |
| AspLEI | GCGC | 3 cut(s) | 93, 881, 1075 |
| AspS9I | GGNCC | 5 cut(s) | 483, 983, 1230, 1251, 1475 |
| AsuC2I | CCSGG | 1 cut(s) | 1271 |
| AsuHPI | GGTGA | 5 cut(s) | 16, 235, 904, 1285, 1450 |
| AvaII | GGWCC | 2 cut(s) | 483, 1230 |
| BaeGI | GKGCMC | 1 cut(s) | 1095 |
| BanII | GRGCYC | 1 cut(s) | 412 |
| BbsI | GAAGAC | 1 cut(s) | 1319 |
| Bbv12I | GWGCWC | 2 cut(s) | 412, 1095 |
| BbvI | GCAGC | 1 cut(s) | 25 |
| BccI | CCATC | 3 cut(s) | 264, 869, 917 |
| BceAI | ACGGC | 1 cut(s) | 1332 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 623, 657 |
| BciT130I | CCWGG | 2 cut(s) | 118, 987 |
| BciVI | GTATCC | 1 cut(s) | 267 |
| BclI | TGATCA | 2 cut(s) | 57, 1059 |
| BcnI | CCSGG | 1 cut(s) | 1271 |
| BcoDI | GTCTC | 1 cut(s) | 1244 |
| BfaI | CTAG | 1 cut(s) | 1088 |
| BfmI | CTRYAG | 2 cut(s) | 11, 1493 |
| BfoI | RGCGCY | 2 cut(s) | 882, 1076 |
| BfuAI | ACCTGC | 1 cut(s) | 1153 |
| BfuI | GTATCC | 1 cut(s) | 267 |
| BisI | GCNGC | 3 cut(s) | 14, 336, 1353 |
| BlsI | GCNGC | 3 cut(s) | 15, 337, 1354 |
| Bme1390I | CCNGG | 3 cut(s) | 118, 987, 1271 |
| Bme18I | GGWCC | 2 cut(s) | 483, 1230 |
| BmgT120I | GGNCC | 5 cut(s) | 483, 983, 1230, 1251, 1475 |
| BmiI | GGNNCC | 5 cut(s) | 73, 469, 555, 1434, 1477 |
| BmrFI | CCNGG | 3 cut(s) | 118, 987, 1271 |
| BmrI | ACTGGG | 1 cut(s) | 535 |
| BmsI | GCATC | 2 cut(s) | 317, 455 |
| BmuI | ACTGGG | 1 cut(s) | 535 |
| BpiI | GAAGAC | 1 cut(s) | 1319 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 542, 574, 948, 980 |
| BpmI | CTGGAG | 1 cut(s) | 1419 |
| BpuEI | CTTGAG | 2 cut(s) | 107, 1355 |
| BpuMI | CCSGG | 1 cut(s) | 1271 |
| BsaAI | YACGTR | 1 cut(s) | 1033 |
| BsaBI | GATNNNNATC | 2 cut(s) | 278, 1364 |
| BsaJI | CCNNGG | 3 cut(s) | 310, 898, 1299 |
| BsaWI | WCCGGW | 1 cut(s) | 37 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 807, 837 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 547, 1049, 1270 |
| Bse1I | ACTGG | 2 cut(s) | 541, 1436 |
| Bse3DI | GCAATG | 1 cut(s) | 1155 |
| Bse8I | GATNNNNATC | 2 cut(s) | 278, 1364 |
| BseBI | CCWGG | 2 cut(s) | 118, 987 |
| BseDI | CCNNGG | 3 cut(s) | 310, 898, 1299 |
| BseGI | GGATG | 5 cut(s) | 234, 275, 279, 332, 843 |
| BseJI | GATNNNNATC | 2 cut(s) | 278, 1364 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 547, 1049, 1270 |
| BseMI | GCAATG | 1 cut(s) | 1155 |
| BseMII | CTCAG | 4 cut(s) | 576, 749, 863, 1255 |
| BseNI | ACTGG | 2 cut(s) | 541, 1436 |
| BseRI | GAGGAG | 4 cut(s) | 350, 535, 873, 1481 |
| BseSI | GKGCMC | 1 cut(s) | 1095 |
| BseXI | GCAGC | 1 cut(s) | 25 |
| BseYI | CCCAGC | 1 cut(s) | 373 |
| BsgI | GTGCAG | 2 cut(s) | 48, 637 |
| Bsh1236I | CGCG | 1 cut(s) | 93 |
| BshFI | GGCC | 4 cut(s) | 985, 1086, 1252, 1476 |
| BsiHKAI | GWGCWC | 2 cut(s) | 412, 1095 |
| BsiSI | CCGG | 2 cut(s) | 38, 1270 |
| BslFI | GGGAC | 1 cut(s) | 1413 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 547, 1049, 1270 |
| BsmAI | GTCTC | 1 cut(s) | 1244 |
| BsmFI | GGGAC | 1 cut(s) | 1413 |
| BsnI | GGCC | 4 cut(s) | 985, 1086, 1252, 1476 |
| Bsp1286I | GDGCHC | 2 cut(s) | 412, 1095 |
| Bsp143I | GATC | 7 cut(s) | 57, 634, 894, 1014, 1059, 1099, 1359 |
| Bsp19I | CCATGG | 1 cut(s) | 898 |
| BspACI | CCGC | 5 cut(s) | 27, 335, 623, 936, 1352 |
| BspANI | GGCC | 4 cut(s) | 985, 1086, 1252, 1476 |
| BspCNI | CTCAG | 4 cut(s) | 577, 748, 862, 1254 |
| BspFNI | CGCG | 1 cut(s) | 93 |
| BspLI | GGNNCC | 5 cut(s) | 73, 469, 555, 1434, 1477 |
| BspMAI | CTGCAG | 1 cut(s) | 15 |
| BspMI | ACCTGC | 1 cut(s) | 1153 |
| BspPI | GGATC | 3 cut(s) | 889, 1022, 1094 |
| BspQI | GCTCTTC | 1 cut(s) | 23 |
| BsrBI | CCGCTC | 2 cut(s) | 938, 1352 |
| BsrDI | GCAATG | 1 cut(s) | 1155 |
| BsrI | ACTGG | 2 cut(s) | 541, 1436 |
| BssECI | CCNNGG | 3 cut(s) | 310, 898, 1299 |
| BssMI | GATC | 7 cut(s) | 57, 634, 894, 1014, 1059, 1099, 1359 |
| BssNAI | GTATAC | 1 cut(s) | 1134 |
| BssT1I | CCWWGG | 2 cut(s) | 898, 1299 |
| Bst1107I | GTATAC | 1 cut(s) | 1134 |
| Bst2UI | CCWGG | 2 cut(s) | 118, 987 |
| Bst4CI | ACNGT | 4 cut(s) | 136, 203, 216, 755 |
| Bst6I | CTCTTC | 1 cut(s) | 23 |
| BstBAI | YACGTR | 1 cut(s) | 1033 |
| BstC8I | GCNNGC | 1 cut(s) | 1474 |
| BstDEI | CTNAG | 6 cut(s) | 585, 675, 735, 849, 887, 1241 |
| BstDSI | CCRYGG | 1 cut(s) | 898 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1047 |
| BstF5I | GGATG | 5 cut(s) | 234, 275, 279, 332, 843 |
| BstFNI | CGCG | 1 cut(s) | 93 |
| BstH2I | RGCGCY | 2 cut(s) | 882, 1076 |
| BstHHI | GCGC | 3 cut(s) | 93, 881, 1075 |
| BstKTI | GATC | 7 cut(s) | 60, 637, 897, 1017, 1062, 1102, 1362 |
| BstMAI | GTCTC | 1 cut(s) | 1244 |
| BstMBI | GATC | 7 cut(s) | 57, 634, 894, 1014, 1059, 1099, 1359 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 13, 467, 629, 935 |
| BstNI | CCWGG | 2 cut(s) | 118, 987 |
| BstSCI | CCNGG | 3 cut(s) | 116, 985, 1269 |
| BstSFI | CTRYAG | 2 cut(s) | 11, 1493 |
| BstSLI | GKGCMC | 1 cut(s) | 1095 |
| BstUI | CGCG | 1 cut(s) | 93 |
| BstV1I | GCAGC | 1 cut(s) | 25 |
| BstV2I | GAAGAC | 1 cut(s) | 1319 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1306 |
| BstZ17I | GTATAC | 1 cut(s) | 1134 |
| BsuI | GTATCC | 1 cut(s) | 267 |
| BsuRI | GGCC | 4 cut(s) | 985, 1086, 1252, 1476 |
| BtgI | CCRYGG | 1 cut(s) | 898 |
| BtsCI | GGATG | 5 cut(s) | 234, 275, 279, 332, 843 |
| BtsI | GCAGTG | 2 cut(s) | 60, 644 |
| BtsIMutI | CAGTG | 4 cut(s) | 60, 582, 644, 1389 |
| BveI | ACCTGC | 1 cut(s) | 1153 |
| Cac8I | GCNNGC | 1 cut(s) | 1474 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1283 |
| CfoI | GCGC | 3 cut(s) | 93, 881, 1075 |
| Cfr13I | GGNCC | 5 cut(s) | 483, 983, 1230, 1251, 1475 |
| CseI | GACGC | 1 cut(s) | 1418 |
| Csp6I | GTAC | 2 cut(s) | 263, 525 |
| CviAII | CATG | 2 cut(s) | 704, 899 |
| CviQI | GTAC | 2 cut(s) | 263, 525 |
| DdeI | CTNAG | 6 cut(s) | 585, 675, 735, 849, 887, 1241 |
| DpnI | GATC | 7 cut(s) | 59, 636, 896, 1016, 1061, 1101, 1361 |
| DpnII | GATC | 7 cut(s) | 57, 634, 894, 1014, 1059, 1099, 1359 |
| Eam1104I | CTCTTC | 1 cut(s) | 23 |
| EarI | CTCTTC | 1 cut(s) | 23 |
| EciI | GGCGGA | 1 cut(s) | 638 |
| Ecl136II | GAGCTC | 1 cut(s) | 410 |
| Eco130I | CCWWGG | 2 cut(s) | 898, 1299 |
| Eco24I | GRGCYC | 1 cut(s) | 412 |
| Eco32I | GATATC | 1 cut(s) | 510 |
| Eco47I | GGWCC | 2 cut(s) | 483, 1230 |
| Eco53kI | GAGCTC | 1 cut(s) | 410 |
| Eco57I | CTGAAG | 1 cut(s) | 1466 |
| EcoICRI | GAGCTC | 1 cut(s) | 410 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1047 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1475 |
| EcoRII | CCWGG | 2 cut(s) | 116, 985 |
| EcoRV | GATATC | 1 cut(s) | 510 |
| EcoT14I | CCWWGG | 2 cut(s) | 898, 1299 |
| EcoT22I | ATGCAT | 1 cut(s) | 448 |
| EcoT38I | GRGCYC | 1 cut(s) | 412 |
| ErhI | CCWWGG | 2 cut(s) | 898, 1299 |
| FaeI | CATG | 2 cut(s) | 707, 902 |
| FaqI | GGGAC | 1 cut(s) | 1413 |
| FatI | CATG | 2 cut(s) | 703, 898 |
| FauI | CCCGC | 1 cut(s) | 943 |
| FauNDI | CATATG | 1 cut(s) | 196 |
| FbaI | TGATCA | 2 cut(s) | 57, 1059 |
| FblI | GTMKAC | 1 cut(s) | 1133 |
| Fnu4HI | GCNGC | 3 cut(s) | 14, 336, 1353 |
| FokI | GGATG | 5 cut(s) | 221, 266, 282, 339, 830 |
| FriOI | GRGCYC | 1 cut(s) | 412 |
| Fsp4HI | GCNGC | 3 cut(s) | 14, 336, 1353 |
| FspBI | CTAG | 1 cut(s) | 1088 |
| GlaI | GCGC | 3 cut(s) | 92, 880, 1074 |
| GluI | GCNGC | 3 cut(s) | 14, 336, 1353 |
| GsaI | CCCAGC | 1 cut(s) | 377 |
| GsuI | CTGGAG | 1 cut(s) | 1419 |
| HaeII | RGCGCY | 2 cut(s) | 882, 1076 |
| HaeIII | GGCC | 4 cut(s) | 985, 1086, 1252, 1476 |
| HapII | CCGG | 2 cut(s) | 38, 1270 |
| HgaI | GACGC | 1 cut(s) | 1418 |
| HhaI | GCGC | 3 cut(s) | 93, 881, 1075 |
| Hin1II | CATG | 2 cut(s) | 707, 902 |
| Hin6I | GCGC | 3 cut(s) | 91, 879, 1073 |
| HinP1I | GCGC | 3 cut(s) | 91, 879, 1073 |
| HincII | GTYRAC | 1 cut(s) | 1486 |
| HindII | GTYRAC | 1 cut(s) | 1486 |
| HindIII | AAGCTT | 2 cut(s) | 677, 1331 |
| HinfI | GANTC | 9 cut(s) | 34, 315, 563, 671, 840, 853, 1126, 1266, 1295 |
| HpaII | CCGG | 2 cut(s) | 38, 1270 |
| HphI | GGTGA | 5 cut(s) | 16, 235, 904, 1285, 1450 |
| Hpy166II | GTNNAC | 5 cut(s) | 232, 860, 1093, 1134, 1486 |
| Hpy188III | TCNNGA | 4 cut(s) | 43, 55, 413, 1123 |
| Hpy8I | GTNNAC | 5 cut(s) | 232, 860, 1093, 1134, 1486 |
| HpyAV | CCTTC | 4 cut(s) | 256, 813, 1226, 1444 |
| HpyCH4III | ACNGT | 4 cut(s) | 136, 203, 216, 755 |
| HpyCH4IV | ACGT | 2 cut(s) | 177, 1032 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 13, 467, 629, 935 |
| HpyF3I | CTNAG | 6 cut(s) | 585, 675, 735, 849, 887, 1241 |
| HpySE526I | ACGT | 2 cut(s) | 177, 1032 |
| Hsp92II | CATG | 2 cut(s) | 707, 902 |
| HspAI | GCGC | 3 cut(s) | 91, 879, 1073 |
| Ksp22I | TGATCA | 2 cut(s) | 57, 1059 |
| Kzo9I | GATC | 7 cut(s) | 57, 634, 894, 1014, 1059, 1099, 1359 |
| LguI | GCTCTTC | 1 cut(s) | 23 |
| Lsp1109I | GCAGC | 1 cut(s) | 25 |
| LweI | GCATC | 2 cut(s) | 317, 455 |
| MaeI | CTAG | 1 cut(s) | 1088 |
| MaeII | ACGT | 2 cut(s) | 177, 1032 |
| MaeIII | GTNAC | 4 cut(s) | 422, 1028, 1273, 1456 |
| MalI | GATC | 7 cut(s) | 59, 636, 896, 1016, 1061, 1101, 1361 |
| MbiI | CCGCTC | 2 cut(s) | 938, 1352 |
| MboI | GATC | 7 cut(s) | 57, 634, 894, 1014, 1059, 1099, 1359 |
| MboII | GAAGA | 9 cut(s) | 10, 122, 133, 504, 591, 1252, 1324, 1369, 1459 |
| MfeI | CAATTG | 1 cut(s) | 1172 |
| MhlI | GDGCHC | 2 cut(s) | 412, 1095 |
| MluCI | AATT | 8 cut(s) | 284, 343, 697, 774, 951, 1020, 1079, 1172 |
| MlyI | GAGTC | 4 cut(s) | 309, 665, 849, 1289 |
| Mph1103I | ATGCAT | 1 cut(s) | 448 |
| MseI | TTAA | 7 cut(s) | 96, 210, 696, 710, 969, 1065, 1206 |
| MslI | CAYNNNNRTG | 1 cut(s) | 429 |
| MspI | CCGG | 2 cut(s) | 38, 1270 |
| MspR9I | CCNGG | 3 cut(s) | 118, 987, 1271 |
| MunI | CAATTG | 1 cut(s) | 1172 |
| MvaI | CCWGG | 2 cut(s) | 118, 987 |
| MvnI | CGCG | 1 cut(s) | 93 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 13, 467, 629, 935 |
| NciI | CCSGG | 1 cut(s) | 1271 |
| NcoI | CCATGG | 1 cut(s) | 898 |
| NdeI | CATATG | 1 cut(s) | 196 |
| NdeII | GATC | 7 cut(s) | 57, 634, 894, 1014, 1059, 1099, 1359 |
| NlaIII | CATG | 2 cut(s) | 707, 902 |
| NlaIV | GGNNCC | 5 cut(s) | 73, 469, 555, 1434, 1477 |
| NmuCI | GTSAC | 3 cut(s) | 422, 1273, 1456 |
| NsiI | ATGCAT | 1 cut(s) | 448 |
| PaqCI | CACCTGC | 1 cut(s) | 1153 |
| PciSI | GCTCTTC | 1 cut(s) | 23 |
| PfeI | GAWTC | 5 cut(s) | 34, 563, 853, 1126, 1266 |
| PflFI | GACNNNGTC | 1 cut(s) | 1279 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 547 |
| PkrI | GCNGC | 3 cut(s) | 15, 337, 1354 |
| PleI | GAGTC | 4 cut(s) | 309, 665, 848, 1289 |
| PpsI | GAGTC | 4 cut(s) | 309, 665, 848, 1289 |
| Ppu21I | YACGTR | 1 cut(s) | 1033 |
| PshBI | ATTAAT | 1 cut(s) | 696 |
| PsiI | TTATAA | 1 cut(s) | 368 |
| Psp124BI | GAGCTC | 1 cut(s) | 412 |
| Psp1406I | AACGTT | 1 cut(s) | 177 |
| Psp6I | CCWGG | 2 cut(s) | 116, 985 |
| PspFI | CCCAGC | 1 cut(s) | 373 |
| PspGI | CCWGG | 2 cut(s) | 116, 985 |
| PspN4I | GGNNCC | 5 cut(s) | 73, 469, 555, 1434, 1477 |
| PspPI | GGNCC | 5 cut(s) | 483, 983, 1230, 1251, 1475 |
| PstI | CTGCAG | 1 cut(s) | 15 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1283 |
| PsyI | GACNNNGTC | 1 cut(s) | 1279 |
| RsaI | GTAC | 2 cut(s) | 264, 526 |
| RsaNI | GTAC | 2 cut(s) | 263, 525 |
| RseI | CAYNNNNRTG | 1 cut(s) | 429 |
| SacI | GAGCTC | 1 cut(s) | 412 |
| SapI | GCTCTTC | 1 cut(s) | 23 |
| SaqAI | TTAA | 7 cut(s) | 96, 210, 696, 710, 969, 1065, 1206 |
| SatI | GCNGC | 3 cut(s) | 14, 336, 1353 |
| Sau3AI | GATC | 7 cut(s) | 57, 634, 894, 1014, 1059, 1099, 1359 |
| Sau96I | GGNCC | 5 cut(s) | 483, 983, 1230, 1251, 1475 |
| SchI | GAGTC | 4 cut(s) | 309, 665, 849, 1289 |
| ScrFI | CCNGG | 3 cut(s) | 118, 987, 1271 |
| SduI | GDGCHC | 2 cut(s) | 412, 1095 |
| SfaNI | GCATC | 2 cut(s) | 317, 455 |
| SfcI | CTRYAG | 2 cut(s) | 11, 1493 |
| SinI | GGWCC | 2 cut(s) | 483, 1230 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 429 |
| SmlI | CTYRAG | 2 cut(s) | 86, 1334 |
| SmoI | CTYRAG | 2 cut(s) | 86, 1334 |
| Sse9I | AATT | 8 cut(s) | 284, 343, 697, 774, 951, 1020, 1079, 1172 |
| SsiI | CCGC | 5 cut(s) | 27, 335, 623, 936, 1352 |
| SspI | AATATT | 1 cut(s) | 100 |
| SspMI | CTAG | 1 cut(s) | 1088 |
| SstI | GAGCTC | 1 cut(s) | 412 |
| StyD4I | CCNGG | 3 cut(s) | 116, 985, 1269 |
| StyI | CCWWGG | 2 cut(s) | 898, 1299 |
| TaaI | ACNGT | 4 cut(s) | 136, 203, 216, 755 |
| TaiI | ACGT | 2 cut(s) | 180, 1035 |
| TaqI | TCGA | 1 cut(s) | 1202 |
| TasI | AATT | 8 cut(s) | 284, 343, 697, 774, 951, 1020, 1079, 1172 |
| TatI | WGTACW | 1 cut(s) | 262 |
| TauI | GCSGC | 2 cut(s) | 338, 1355 |
| TfiI | GAWTC | 5 cut(s) | 34, 563, 853, 1126, 1266 |
| Tru1I | TTAA | 7 cut(s) | 96, 210, 696, 710, 969, 1065, 1206 |
| Tru9I | TTAA | 7 cut(s) | 96, 210, 696, 710, 969, 1065, 1206 |
| TscAI | CASTG | 4 cut(s) | 67, 589, 651, 1396 |
| TseFI | GTSAC | 3 cut(s) | 422, 1273, 1456 |
| TseI | GCWGC | 1 cut(s) | 13 |
| Tsp45I | GTSAC | 3 cut(s) | 422, 1273, 1456 |
| TspDTI | ATGAA | 4 cut(s) | 10, 238, 621, 1166 |
| TspGWI | ACGGA | 1 cut(s) | 1362 |
| TspRI | CASTG | 4 cut(s) | 67, 589, 651, 1396 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1279 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 547 |
| VneI | GTGCAC | 1 cut(s) | 1091 |
| VpaK11BI | GGWCC | 2 cut(s) | 483, 1230 |
| VspI | ATTAAT | 1 cut(s) | 696 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1047 |
| XapI | RAATTY | 3 cut(s) | 343, 774, 1079 |
| XmiI | GTMKAC | 1 cut(s) | 1133 |
| XspI | CTAG | 1 cut(s) | 1088 |
| Zsp2I | ATGCAT | 1 cut(s) | 448 |