Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 413 |
| AccB1I | GGYRCC | 2 cut(s) | 752, 814 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 55 |
| AccII | CGCG | 2 cut(s) | 845, 1059 |
| AciI | CCGC | 1 cut(s) | 114 |
| AclWI | GGATC | 1 cut(s) | 36 |
| AcoI | YGGCCR | 2 cut(s) | 193, 625 |
| AcyI | GRCGYC | 1 cut(s) | 753 |
| AfaI | GTAC | 4 cut(s) | 723, 734, 826, 994 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 55, 191, 279, 634 |
| AgsI | TTSAA | 2 cut(s) | 364, 1064 |
| AjiI | CACGTC | 1 cut(s) | 962 |
| AjnI | CCWGG | 3 cut(s) | 24, 272, 735 |
| AluBI | AGCT | 1 cut(s) | 587 |
| AluI | AGCT | 1 cut(s) | 587 |
| Alw26I | GTCTC | 2 cut(s) | 502, 922 |
| AlwI | GGATC | 1 cut(s) | 36 |
| AoxI | GGCC | 3 cut(s) | 193, 625, 939 |
| ApeKI | GCWGC | 3 cut(s) | 382, 665, 668 |
| AspLEI | GCGC | 2 cut(s) | 89, 755 |
| AspS9I | GGNCC | 1 cut(s) | 483 |
| AsuC2I | CCSGG | 1 cut(s) | 192 |
| AvaII | GGWCC | 1 cut(s) | 483 |
| BanI | GGYRCC | 2 cut(s) | 752, 814 |
| BbsI | GAAGAC | 1 cut(s) | 276 |
| BbvI | GCAGC | 3 cut(s) | 369, 677, 680 |
| BccI | CCATC | 5 cut(s) | 49, 288, 519, 617, 1016 |
| BceAI | ACGGC | 1 cut(s) | 612 |
| BciT130I | CCWGG | 3 cut(s) | 26, 274, 737 |
| BcnI | CCSGG | 1 cut(s) | 192 |
| BcoDI | GTCTC | 2 cut(s) | 502, 922 |
| BfaI | CTAG | 3 cut(s) | 315, 435, 704 |
| BfmI | CTRYAG | 1 cut(s) | 909 |
| BfoI | RGCGCY | 1 cut(s) | 756 |
| BglII | AGATCT | 1 cut(s) | 997 |
| BisI | GCNGC | 4 cut(s) | 114, 383, 666, 669 |
| BlsI | GCNGC | 4 cut(s) | 115, 384, 667, 670 |
| BmcAI | AGTACT | 1 cut(s) | 826 |
| Bme1390I | CCNGG | 4 cut(s) | 26, 192, 274, 737 |
| Bme18I | GGWCC | 1 cut(s) | 483 |
| BmgBI | CACGTC | 1 cut(s) | 962 |
| BmgT120I | GGNCC | 1 cut(s) | 483 |
| BmiI | GGNNCC | 2 cut(s) | 754, 816 |
| BmrFI | CCNGG | 4 cut(s) | 26, 192, 274, 737 |
| BmsI | GCATC | 3 cut(s) | 147, 364, 681 |
| BpiI | GAAGAC | 1 cut(s) | 276 |
| BpmI | CTGGAG | 1 cut(s) | 526 |
| BpuEI | CTTGAG | 1 cut(s) | 761 |
| BpuMI | CCSGG | 1 cut(s) | 192 |
| BsaHI | GRCGYC | 1 cut(s) | 753 |
| BsaI | GGTCTC | 2 cut(s) | 502, 922 |
| BsaJI | CCNNGG | 2 cut(s) | 24, 272 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 55, 191, 279, 634 |
| Bse1I | ACTGG | 3 cut(s) | 14, 196, 832 |
| BseBI | CCWGG | 3 cut(s) | 26, 274, 737 |
| BseDI | CCNNGG | 2 cut(s) | 24, 272 |
| BseGI | GGATG | 5 cut(s) | 60, 299, 355, 530, 628 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 55, 191, 279, 634 |
| BseMII | CTCAG | 2 cut(s) | 933, 1097 |
| BseNI | ACTGG | 3 cut(s) | 14, 196, 832 |
| BseXI | GCAGC | 3 cut(s) | 369, 677, 680 |
| Bsh1236I | CGCG | 2 cut(s) | 845, 1059 |
| BshFI | GGCC | 3 cut(s) | 195, 627, 941 |
| BshNI | GGYRCC | 2 cut(s) | 752, 814 |
| BsiSI | CCGG | 2 cut(s) | 192, 772 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 55, 191, 279, 634 |
| BsmAI | GTCTC | 2 cut(s) | 502, 922 |
| BsmI | GAATGC | 1 cut(s) | 983 |
| BsnI | GGCC | 3 cut(s) | 195, 627, 941 |
| Bso31I | GGTCTC | 2 cut(s) | 502, 922 |
| Bsp1407I | TGTACA | 1 cut(s) | 992 |
| Bsp143I | GATC | 4 cut(s) | 16, 28, 572, 997 |
| BspACI | CCGC | 1 cut(s) | 114 |
| BspANI | GGCC | 3 cut(s) | 195, 627, 941 |
| BspCNI | CTCAG | 2 cut(s) | 932, 1096 |
| BspFNI | CGCG | 2 cut(s) | 845, 1059 |
| BspLI | GGNNCC | 2 cut(s) | 754, 816 |
| BspPI | GGATC | 1 cut(s) | 36 |
| BspT107I | GGYRCC | 2 cut(s) | 752, 814 |
| BspTNI | GGTCTC | 2 cut(s) | 502, 922 |
| BsrGI | TGTACA | 1 cut(s) | 992 |
| BsrI | ACTGG | 3 cut(s) | 14, 196, 832 |
| BssECI | CCNNGG | 2 cut(s) | 24, 272 |
| BssMI | GATC | 4 cut(s) | 16, 28, 572, 997 |
| BssNI | GRCGYC | 1 cut(s) | 753 |
| Bst2UI | CCWGG | 3 cut(s) | 26, 274, 737 |
| Bst4CI | ACNGT | 1 cut(s) | 762 |
| BstACI | GRCGYC | 1 cut(s) | 753 |
| BstAUI | TGTACA | 1 cut(s) | 992 |
| BstDEI | CTNAG | 3 cut(s) | 821, 919, 1083 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 277 |
| BstF5I | GGATG | 5 cut(s) | 60, 299, 355, 530, 628 |
| BstFNI | CGCG | 2 cut(s) | 845, 1059 |
| BstH2I | RGCGCY | 1 cut(s) | 756 |
| BstHHI | GCGC | 2 cut(s) | 89, 755 |
| BstKTI | GATC | 4 cut(s) | 19, 31, 575, 1000 |
| BstMAI | GTCTC | 2 cut(s) | 502, 922 |
| BstMBI | GATC | 4 cut(s) | 16, 28, 572, 997 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 157 |
| BstNI | CCWGG | 3 cut(s) | 26, 274, 737 |
| BstSCI | CCNGG | 4 cut(s) | 24, 190, 272, 735 |
| BstSFI | CTRYAG | 1 cut(s) | 909 |
| BstUI | CGCG | 2 cut(s) | 845, 1059 |
| BstV1I | GCAGC | 3 cut(s) | 369, 677, 680 |
| BstV2I | GAAGAC | 1 cut(s) | 276 |
| BstX2I | RGATCY | 2 cut(s) | 28, 997 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1077 |
| BstYI | RGATCY | 2 cut(s) | 28, 997 |
| BsuRI | GGCC | 3 cut(s) | 195, 627, 941 |
| BtgZI | GCGATG | 1 cut(s) | 289 |
| BtrI | CACGTC | 1 cut(s) | 962 |
| BtsCI | GGATG | 5 cut(s) | 60, 299, 355, 530, 628 |
| BtsI | GCAGTG | 1 cut(s) | 146 |
| BtsIMutI | CAGTG | 3 cut(s) | 146, 203, 1091 |
| CfoI | GCGC | 2 cut(s) | 89, 755 |
| Cfr13I | GGNCC | 1 cut(s) | 483 |
| CseI | GACGC | 1 cut(s) | 851 |
| CsiI | ACCWGGT | 1 cut(s) | 735 |
| Csp6I | GTAC | 4 cut(s) | 722, 733, 825, 993 |
| CviAII | CATG | 4 cut(s) | 64, 102, 368, 466 |
| CviJI | RGCY | 8 cut(s) | 5, 195, 298, 587, 627, 775, 875, 941 |
| CviKI_1 | RGCY | 8 cut(s) | 5, 195, 298, 587, 627, 775, 875, 941 |
| CviQI | GTAC | 4 cut(s) | 722, 733, 825, 993 |
| DdeI | CTNAG | 3 cut(s) | 821, 919, 1083 |
| DinI | GGCGCC | 1 cut(s) | 754 |
| DpnI | GATC | 4 cut(s) | 18, 30, 574, 999 |
| DpnII | GATC | 4 cut(s) | 16, 28, 572, 997 |
| DraI | TTTAAA | 1 cut(s) | 891 |
| EaeI | YGGCCR | 2 cut(s) | 193, 625 |
| Eco31I | GGTCTC | 2 cut(s) | 502, 922 |
| Eco47I | GGWCC | 1 cut(s) | 483 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 277 |
| EcoRII | CCWGG | 3 cut(s) | 24, 272, 735 |
| EgeI | GGCGCC | 1 cut(s) | 754 |
| EheI | GGCGCC | 1 cut(s) | 754 |
| FaeI | CATG | 4 cut(s) | 67, 105, 371, 469 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 410, 442 |
| FatI | CATG | 4 cut(s) | 63, 101, 367, 465 |
| FauNDI | CATATG | 2 cut(s) | 577, 1072 |
| Fnu4HI | GCNGC | 4 cut(s) | 114, 383, 666, 669 |
| FokI | GGATG | 5 cut(s) | 67, 306, 342, 537, 635 |
| Fsp4HI | GCNGC | 4 cut(s) | 114, 383, 666, 669 |
| FspBI | CTAG | 3 cut(s) | 315, 435, 704 |
| GlaI | GCGC | 2 cut(s) | 88, 754 |
| GluI | GCNGC | 4 cut(s) | 114, 383, 666, 669 |
| GsuI | CTGGAG | 1 cut(s) | 526 |
| HaeII | RGCGCY | 1 cut(s) | 756 |
| HaeIII | GGCC | 3 cut(s) | 195, 627, 941 |
| HapII | CCGG | 2 cut(s) | 192, 772 |
| HgaI | GACGC | 1 cut(s) | 851 |
| HhaI | GCGC | 2 cut(s) | 89, 755 |
| Hin1I | GRCGYC | 1 cut(s) | 753 |
| Hin1II | CATG | 4 cut(s) | 67, 105, 371, 469 |
| Hin6I | GCGC | 2 cut(s) | 87, 753 |
| HinP1I | GCGC | 2 cut(s) | 87, 753 |
| HinfI | GANTC | 6 cut(s) | 59, 332, 560, 743, 868, 1081 |
| HpaII | CCGG | 2 cut(s) | 192, 772 |
| Hpy166II | GTNNAC | 1 cut(s) | 1089 |
| Hpy188III | TCNNGA | 6 cut(s) | 228, 315, 336, 505, 778, 789 |
| Hpy8I | GTNNAC | 1 cut(s) | 1089 |
| Hpy99I | CGWCG | 1 cut(s) | 264 |
| HpyAV | CCTTC | 3 cut(s) | 450, 508, 841 |
| HpyCH4III | ACNGT | 1 cut(s) | 762 |
| HpyCH4IV | ACGT | 2 cut(s) | 259, 961 |
| HpyCH4V | TGCA | 5 cut(s) | 67, 355, 948, 959, 981 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 157 |
| HpyF3I | CTNAG | 3 cut(s) | 821, 919, 1083 |
| HpySE526I | ACGT | 2 cut(s) | 259, 961 |
| Hsp92I | GRCGYC | 1 cut(s) | 753 |
| Hsp92II | CATG | 4 cut(s) | 67, 105, 371, 469 |
| HspAI | GCGC | 2 cut(s) | 87, 753 |
| KasI | GGCGCC | 1 cut(s) | 752 |
| Kzo9I | GATC | 4 cut(s) | 16, 28, 572, 997 |
| Lsp1109I | GCAGC | 3 cut(s) | 369, 677, 680 |
| LweI | GCATC | 3 cut(s) | 147, 364, 681 |
| MabI | ACCWGGT | 1 cut(s) | 735 |
| MaeI | CTAG | 3 cut(s) | 315, 435, 704 |
| MaeII | ACGT | 2 cut(s) | 259, 961 |
| MaeIII | GTNAC | 2 cut(s) | 200, 641 |
| MalI | GATC | 4 cut(s) | 18, 30, 574, 999 |
| MboI | GATC | 4 cut(s) | 16, 28, 572, 997 |
| MboII | GAAGA | 5 cut(s) | 11, 276, 383, 891, 959 |
| MfeI | CAATTG | 3 cut(s) | 728, 832, 943 |
| MflI | RGATCY | 2 cut(s) | 28, 997 |
| MluCI | AATT | 7 cut(s) | 375, 553, 602, 728, 832, 895, 943 |
| Mly113I | GGCGCC | 1 cut(s) | 753 |
| MlyI | GAGTC | 1 cut(s) | 1075 |
| MmeI | TCCRAC | 2 cut(s) | 819, 1010 |
| MnlI | CCTC | 7 cut(s) | 163, 218, 319, 805, 844, 907, 1021 |
| MseI | TTAA | 2 cut(s) | 234, 890 |
| MslI | CAYNNNNRTG | 2 cut(s) | 464, 1075 |
| MspI | CCGG | 2 cut(s) | 192, 772 |
| MspR9I | CCNGG | 4 cut(s) | 26, 192, 274, 737 |
| MunI | CAATTG | 3 cut(s) | 728, 832, 943 |
| Mva1269I | GAATGC | 1 cut(s) | 983 |
| MvaI | CCWGG | 3 cut(s) | 26, 274, 737 |
| MvnI | CGCG | 2 cut(s) | 845, 1059 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 157 |
| NarI | GGCGCC | 1 cut(s) | 753 |
| NciI | CCSGG | 1 cut(s) | 192 |
| NdeI | CATATG | 2 cut(s) | 577, 1072 |
| NdeII | GATC | 4 cut(s) | 16, 28, 572, 997 |
| NlaIII | CATG | 4 cut(s) | 67, 105, 371, 469 |
| NlaIV | GGNNCC | 2 cut(s) | 754, 816 |
| NmuCI | GTSAC | 1 cut(s) | 641 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 625 |
| PctI | GAATGC | 1 cut(s) | 983 |
| PfeI | GAWTC | 5 cut(s) | 59, 332, 560, 743, 868 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 55 |
| PkrI | GCNGC | 4 cut(s) | 115, 384, 667, 670 |
| PleI | GAGTC | 1 cut(s) | 1075 |
| PluTI | GGCGCC | 1 cut(s) | 756 |
| PpsI | GAGTC | 1 cut(s) | 1075 |
| PsiI | TTATAA | 1 cut(s) | 413 |
| Psp6I | CCWGG | 3 cut(s) | 24, 272, 735 |
| PspGI | CCWGG | 3 cut(s) | 24, 272, 735 |
| PspN4I | GGNNCC | 2 cut(s) | 754, 816 |
| PspPI | GGNCC | 1 cut(s) | 483 |
| PsuI | RGATCY | 2 cut(s) | 28, 997 |
| RsaI | GTAC | 4 cut(s) | 723, 734, 826, 994 |
| RsaNI | GTAC | 4 cut(s) | 722, 733, 825, 993 |
| RseI | CAYNNNNRTG | 2 cut(s) | 464, 1075 |
| SaqAI | TTAA | 2 cut(s) | 234, 890 |
| SatI | GCNGC | 4 cut(s) | 114, 383, 666, 669 |
| Sau3AI | GATC | 4 cut(s) | 16, 28, 572, 997 |
| Sau96I | GGNCC | 1 cut(s) | 483 |
| ScaI | AGTACT | 1 cut(s) | 826 |
| SchI | GAGTC | 1 cut(s) | 1075 |
| ScrFI | CCNGG | 4 cut(s) | 26, 192, 274, 737 |
| SexAI | ACCWGGT | 1 cut(s) | 735 |
| SfaNI | GCATC | 3 cut(s) | 147, 364, 681 |
| SfcI | CTRYAG | 1 cut(s) | 909 |
| SfoI | GGCGCC | 1 cut(s) | 754 |
| SinI | GGWCC | 1 cut(s) | 483 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 464, 1075 |
| SmlI | CTYRAG | 1 cut(s) | 776 |
| SmoI | CTYRAG | 1 cut(s) | 776 |
| Sse9I | AATT | 7 cut(s) | 375, 553, 602, 728, 832, 895, 943 |
| SsiI | CCGC | 1 cut(s) | 114 |
| SspDI | GGCGCC | 1 cut(s) | 752 |
| SspMI | CTAG | 3 cut(s) | 315, 435, 704 |
| StyD4I | CCNGG | 4 cut(s) | 24, 190, 272, 735 |
| TaaI | ACNGT | 1 cut(s) | 762 |
| TaiI | ACGT | 2 cut(s) | 262, 964 |
| TaqI | TCGA | 4 cut(s) | 615, 790, 871, 1032 |
| TaqII | GACCGA | 1 cut(s) | 526 |
| TasI | AATT | 7 cut(s) | 375, 553, 602, 728, 832, 895, 943 |
| TatI | WGTACW | 2 cut(s) | 824, 992 |
| TauI | GCSGC | 1 cut(s) | 116 |
| TfiI | GAWTC | 5 cut(s) | 59, 332, 560, 743, 868 |
| Tru1I | TTAA | 2 cut(s) | 234, 890 |
| Tru9I | TTAA | 2 cut(s) | 234, 890 |
| TscAI | CASTG | 3 cut(s) | 153, 203, 1091 |
| TseFI | GTSAC | 1 cut(s) | 641 |
| TseI | GCWGC | 3 cut(s) | 382, 665, 668 |
| Tsp45I | GTSAC | 1 cut(s) | 641 |
| TspDTI | ATGAA | 4 cut(s) | 64, 384, 891, 1031 |
| TspRI | CASTG | 3 cut(s) | 153, 203, 1091 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 55 |
| VpaK11BI | GGWCC | 1 cut(s) | 483 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 277 |
| XbaI | TCTAGA | 1 cut(s) | 314 |
| XspI | CTAG | 3 cut(s) | 315, 435, 704 |
| ZrmI | AGTACT | 1 cut(s) | 826 |