RchiOBHm_Chr7g0217181

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000423
Physical Location & Seq
Forward (+)
35064240 .. 35066925
2686 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19435

Sequence Viewer

Length: 1245 bp
ATGGCGCTTTTGCAGCTTTTCATCACAGCCTCAATTCCGGTCTTGAAAGTGCTCTTGATCACTGCACTAGGTTCCTATCTTGCACTTGAGCGCGTTAATATTCTGGGGGAAGAAACCAGGAAGAACATGAACACTGTTGTTTTTTATGTATTCAGTCCAGCTCTGATTGGAACTAACCTTGCCCAGACTATTACATATGACAGTATGATTAAACTGTGGTTTATGCCTGTGAACATCTTAATCACGTTTATTGTTGGTTCTGTACTTGGATGGATACTCATCCAATTAGCAAGACCACCTCCACATCTCCGAGGCCTCATTTTGGGTTGCTGTGCCGCTGGAAATTTGGGGAACTTGCTCCTCATTATAATCCCAGCAGTCTGTAAAGAGAAGGGAAGCCCATTTGGAGCACCTGATGTCTGTCACACTTATGGAATGGCTTATGCATCACTTTCACTGGCGATAGGAGCCATTTACCTTTGGTCCTATGTGTATAACGTTGTGCGGATATCTTCAAAGAGGAGTACCCAAGACTCCCCCCAGTCTTTGGAGGAACCCTCCGAATCAAATCAAGTAAGTTGCACTGAGCCTCTGCTTTCTTCCAAGGAATATGGGATAGTGGAGGACAATACCGATCAGTATGCACTGCCCTGCACTATATCTCAACAAGACTCTAAGATAGAACCTCCGAGTAAATCCAAGAACTGTATAGTGAGGATGTGCCAAATGCTTAATCTTAAAACAATATTTGCCCCATCAACTACTGGAGCGATTGTTGGGTTTACAATTGGAGTCATCCCTCAGATTCGAAAACTAATGATAGGAGATGATGCTCCTCTGCGGGTGATCCAAGACACTACTTACTTGTTGGGAGATGGAGCTATTCCCGCTCTCACTTTGATAATTGGAGGAAACCTCCTTAAAGGTTTGAGAGGGTCAGGAATACAGAAATCTATTGTTGTTGGGATCATATTCGTTCGTTACATGTTCCTGCCTCTTACAGGCATATTGATCATTAAAGGGGCGCTAAAATTCGGCCTAGTGCACTCTGATCCTTTATATCTATTTGTTCTCCTGATTCAGTATACACTCCCACCTGCAATGAACATAGGAACCATCACCCAATTGTTCGGAGCGGGAGAGAACGAATGTTCAGTAATCATGCTGTGGACTTATGTATTTGCTTCAGTATCACTTACTTTATGGTCTGCCTTCTTTATGTGGTTTGTGGCCAGGCAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0044425 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

414

Amino Acids

45.23

Weight (kDa)

8.49

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 10 - 402 4.9e-80 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000423)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76520 AT1G76520 AT1G76530 AT1G76530 AT1G76530
fragaria_vesca FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26311 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26330 FvH4_5g26370 FvH4_5g26370
malus_domestica MD05G1225800.v1.1 MD06G1002600.v1.1 MD06G1002700.v1.1
prunus_persica Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004000_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004100_v2.0.a1 Prupe.5G004300_v2.0.a1
pyrus_communis pycom05g20790 pycom06g00270 pycom06g00280
rosa_chinensis RchiOBHm_Chr7g0217021 RchiOBHm_Chr7g0217081 RchiOBHm_Chr7g0217111 RchiOBHm_Chr7g0217141 RchiOBHm_Chr7g0217181 RchiOBHm_Chr7g0219341
rosa_laevigata RLG00000002504 RLG00000002506 RLG00000002510 RLG00000002512
rosa_multiflora Rmu_sc0001725.1_g000022 Rmu_sc0002460.1_g000002 Rmu_sc0003132.1_g000001 Rmu_sc0007359.1_g000005 Rmu_sc0007359.1_g000009 Rmu_sc0018492.1_g000001 Rmu_sc0039353.1_g000001
rosa_roxburghii Rroxscaffold_3G00242520 Rroxscaffold_3G00242540 Rroxscaffold_3G00242560 Rroxscaffold_3G00242600
rosa_rugosa Rorug07G0165700 Rorug07G0165700 Rorug07G0165800 Rorug07G0165800 Rorug07G0166400 Rorug07G0166500 Rorug07G0166600 Rorug07G0166700 Rorug07G0166800 Rorug07G0166900 Rorug07G0167000 Rorug07G0167000 Rorug07G0167100
rosa_samantha Rh7AG305200 Rh7AG305300 Rh7AG305700 Rh7AG305900 Rh7AG327400 Rh7CG324200 Rh7CG324400 Rh7CG324900 Rh7CG344700 Rh7DG305400 Rh7DG305500 Rh7DG305800 Rh7DG323000
rosa_wichuraiana Rw0G004530 Rw3G011620 Rw7G025930 Rw7G025960 Rw7G025970 Rw7G025990 Rw7G027580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AarI CACCTGC 1 cut(s) 1105
Acc36I ACCTGC 1 cut(s) 1105
AccB7I CCANNNNNTGG 1 cut(s) 547
AccBSI CCGCTC 2 cut(s) 890, 1136
AccI GTMKAC 1 cut(s) 1085
AccII CGCG 1 cut(s) 93
AciI CCGC 5 cut(s) 336, 505, 841, 888, 1136
AclI AACGTT 1 cut(s) 498
AclWI GGATC 3 cut(s) 841, 974, 1046
AcoI YGGCCR 1 cut(s) 1230
AcsI RAATTY 2 cut(s) 343, 1031
AcuI CTGAAG 1 cut(s) 1170
AfaI GTAC 2 cut(s) 264, 526
AfiI CCNNNNNNNGG 3 cut(s) 322, 547, 1001
AflIII ACRYGT 1 cut(s) 984
AgsI TTSAA 2 cut(s) 46, 516
AjnI CCWGG 2 cut(s) 116, 1232
AluBI AGCT 3 cut(s) 16, 161, 881
AluI AGCT 3 cut(s) 16, 161, 881
Alw21I GWGCWC 3 cut(s) 54, 412, 1047
Alw44I GTGCAC 1 cut(s) 1043
AlwI GGATC 3 cut(s) 841, 974, 1046
AoxI GGCC 3 cut(s) 313, 1036, 1230
ApaLI GTGCAC 1 cut(s) 1043
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 2 cut(s) 343, 1031
AspLEI GCGC 3 cut(s) 7, 93, 1027
AspS9I GGNCC 1 cut(s) 483
AsuHPI GGTGA 2 cut(s) 856, 1111
AsuII TTCGAA 1 cut(s) 808
AvaII GGWCC 1 cut(s) 483
BaeGI GKGCMC 1 cut(s) 1047
BalI TGGCCA 1 cut(s) 1232
Bbv12I GWGCWC 3 cut(s) 54, 412, 1047
BbvI GCAGC 1 cut(s) 25
BccI CCATC 4 cut(s) 264, 763, 869, 1124
BcgI CGANNNNNNTGC 2 cut(s) 623, 657
BciT130I CCWGG 2 cut(s) 118, 1234
BciVI GTATCC 1 cut(s) 267
BclI TGATCA 2 cut(s) 57, 1011
BfaI CTAG 2 cut(s) 68, 1040
BfoI RGCGCY 2 cut(s) 8, 1028
BfuAI ACCTGC 1 cut(s) 1105
BfuI GTATCC 1 cut(s) 267
BisI GCNGC 2 cut(s) 14, 336
BlsI GCNGC 2 cut(s) 15, 337
Bme1390I CCNGG 2 cut(s) 118, 1234
Bme18I GGWCC 1 cut(s) 483
BmgT120I GGNCC 1 cut(s) 483
BmiI GGNNCC 4 cut(s) 73, 469, 555, 1114
BmrFI CCNGG 2 cut(s) 118, 1234
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 2 cut(s) 455, 820
BmuI ACTGGG 1 cut(s) 535
BplI GAGNNNNNCTC 4 cut(s) 542, 574, 900, 932
BpmI CTGGAG 1 cut(s) 786
Bpu14I TTCGAA 1 cut(s) 808
BpuEI CTTGAG 1 cut(s) 107
BsaBI GATNNNNATC 1 cut(s) 278
BsaJI CCNNGG 2 cut(s) 310, 603
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 759, 789
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 547, 1001
Bse1I ACTGG 3 cut(s) 462, 541, 769
Bse3DI GCAATG 1 cut(s) 1107
Bse8I GATNNNNATC 1 cut(s) 278
BseBI CCWGG 2 cut(s) 118, 1234
BseDI CCNNGG 2 cut(s) 310, 603
BseGI GGATG 4 cut(s) 275, 279, 723, 795
BseJI GATNNNNATC 1 cut(s) 278
BseLI CCNNNNNNNGG 3 cut(s) 322, 547, 1001
BseMI GCAATG 1 cut(s) 1107
BseMII CTCAG 2 cut(s) 576, 815
BseNI ACTGG 3 cut(s) 462, 541, 769
BseRI GAGGAG 3 cut(s) 350, 535, 825
BseSI GKGCMC 1 cut(s) 1047
BseXI GCAGC 1 cut(s) 25
BseYI CCCAGC 1 cut(s) 373
BsgI GTGCAG 2 cut(s) 48, 637
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 3 cut(s) 315, 1038, 1232
BsiHKAI GWGCWC 3 cut(s) 54, 412, 1047
BsiSI CCGG 1 cut(s) 38
BslI CCNNNNNNNGG 3 cut(s) 322, 547, 1001
BsnI GGCC 3 cut(s) 315, 1038, 1232
Bsp119I TTCGAA 1 cut(s) 808
Bsp1286I GDGCHC 3 cut(s) 54, 412, 1047
Bsp143I GATC 6 cut(s) 57, 634, 846, 966, 1011, 1051
BspACI CCGC 5 cut(s) 336, 505, 841, 888, 1136
BspANI GGCC 3 cut(s) 315, 1038, 1232
BspCNI CTCAG 2 cut(s) 577, 814
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 4 cut(s) 73, 469, 555, 1114
BspMI ACCTGC 1 cut(s) 1105
BspPI GGATC 3 cut(s) 841, 974, 1046
BspT104I TTCGAA 1 cut(s) 808
BsrBI CCGCTC 2 cut(s) 890, 1136
BsrDI GCAATG 1 cut(s) 1107
BsrI ACTGG 3 cut(s) 462, 541, 769
BssECI CCNNGG 2 cut(s) 310, 603
BssMI GATC 6 cut(s) 57, 634, 846, 966, 1011, 1051
BssNAI GTATAC 1 cut(s) 1086
BssT1I CCWWGG 1 cut(s) 603
Bst1107I GTATAC 1 cut(s) 1086
Bst2UI CCWGG 2 cut(s) 118, 1234
Bst4CI ACNGT 4 cut(s) 136, 203, 216, 707
BstBI TTCGAA 1 cut(s) 808
BstDEI CTNAG 3 cut(s) 585, 675, 801
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 4 cut(s) 275, 279, 723, 795
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 2 cut(s) 8, 1028
BstHHI GCGC 3 cut(s) 7, 93, 1027
BstKTI GATC 6 cut(s) 60, 637, 849, 969, 1014, 1054
BstMBI GATC 6 cut(s) 57, 634, 846, 966, 1011, 1051
BstMWI GCNNNNNNNGC 3 cut(s) 13, 467, 887
BstNI CCWGG 2 cut(s) 118, 1234
BstNSI RCATGY 1 cut(s) 988
BstSCI CCNGG 2 cut(s) 116, 1232
BstSLI GKGCMC 1 cut(s) 1047
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 25
BstZ17I GTATAC 1 cut(s) 1086
BsuI GTATCC 1 cut(s) 267
BsuRI GGCC 3 cut(s) 315, 1038, 1232
BtsCI GGATG 4 cut(s) 275, 279, 723, 795
BtsI GCAGTG 2 cut(s) 60, 644
BtsIMutI CAGTG 5 cut(s) 60, 132, 455, 582, 644
BveI ACCTGC 1 cut(s) 1105
CfoI GCGC 3 cut(s) 7, 93, 1027
Cfr13I GGNCC 1 cut(s) 483
Csp6I GTAC 2 cut(s) 263, 525
CviAII CATG 3 cut(s) 127, 985, 1162
CviQI GTAC 2 cut(s) 263, 525
DdeI CTNAG 3 cut(s) 585, 675, 801
DpnI GATC 6 cut(s) 59, 636, 848, 968, 1013, 1053
DpnII GATC 6 cut(s) 57, 634, 846, 966, 1011, 1051
EaeI YGGCCR 1 cut(s) 1230
Eco130I CCWWGG 1 cut(s) 603
Eco147I AGGCCT 1 cut(s) 315
Eco32I GATATC 1 cut(s) 510
Eco47I GGWCC 1 cut(s) 483
Eco57I CTGAAG 1 cut(s) 1170
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoRII CCWGG 2 cut(s) 116, 1232
EcoRV GATATC 1 cut(s) 510
EcoT14I CCWWGG 1 cut(s) 603
EcoT22I ATGCAT 1 cut(s) 448
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 3 cut(s) 130, 988, 1165
FatI CATG 3 cut(s) 126, 984, 1161
FauI CCCGC 3 cut(s) 834, 895, 1129
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 2 cut(s) 57, 1011
FblI GTMKAC 1 cut(s) 1085
Fnu4HI GCNGC 2 cut(s) 14, 336
FokI GGATG 4 cut(s) 266, 282, 730, 782
Fsp4HI GCNGC 2 cut(s) 14, 336
FspBI CTAG 2 cut(s) 68, 1040
GlaI GCGC 3 cut(s) 6, 92, 1026
GluI GCNGC 2 cut(s) 14, 336
GsaI CCCAGC 1 cut(s) 377
GsuI CTGGAG 1 cut(s) 786
HaeII RGCGCY 2 cut(s) 8, 1028
HaeIII GGCC 3 cut(s) 315, 1038, 1232
HapII CCGG 1 cut(s) 38
HhaI GCGC 3 cut(s) 7, 93, 1027
Hin1II CATG 3 cut(s) 130, 988, 1165
Hin6I GCGC 3 cut(s) 5, 91, 1025
HinP1I GCGC 3 cut(s) 5, 91, 1025
HinfI GANTC 6 cut(s) 533, 563, 671, 792, 805, 1078
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 856, 1111
Hpy166II GTNNAC 5 cut(s) 232, 783, 1045, 1086, 1170
Hpy188I TCNGA 7 cut(s) 165, 311, 562, 690, 804, 1051, 1133
Hpy188III TCNNGA 4 cut(s) 43, 55, 939, 1075
Hpy8I GTNNAC 5 cut(s) 232, 783, 1045, 1086, 1170
HpyAV CCTTC 2 cut(s) 385, 1222
HpyCH4III ACNGT 4 cut(s) 136, 203, 216, 707
HpyCH4IV ACGT 2 cut(s) 245, 498
HpyCH4V TGCA 9 cut(s) 13, 65, 83, 446, 582, 644, 654, 1045, 1100
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 467, 887
HpyF3I CTNAG 3 cut(s) 585, 675, 801
HpySE526I ACGT 2 cut(s) 245, 498
Hsp92II CATG 3 cut(s) 130, 988, 1165
HspAI GCGC 3 cut(s) 5, 91, 1025
Ksp22I TGATCA 2 cut(s) 57, 1011
Kzo9I GATC 6 cut(s) 57, 634, 846, 966, 1011, 1051
LmnI GCTCC 7 cut(s) 363, 407, 467, 767, 838, 878, 1133
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 2 cut(s) 455, 820
MaeI CTAG 2 cut(s) 68, 1040
MaeII ACGT 2 cut(s) 245, 498
MaeIII GTNAC 2 cut(s) 422, 980
MalI GATC 6 cut(s) 59, 636, 848, 968, 1013, 1053
MbiI CCGCTC 2 cut(s) 890, 1136
MboI GATC 6 cut(s) 57, 634, 846, 966, 1011, 1051
MboII GAAGA 4 cut(s) 122, 133, 504, 591
MfeI CAATTG 2 cut(s) 786, 1124
MhlI GDGCHC 3 cut(s) 54, 412, 1047
MlsI TGGCCA 1 cut(s) 1232
MluCI AATT 7 cut(s) 33, 284, 343, 786, 903, 1031, 1124
MluNI TGGCCA 1 cut(s) 1232
MlyI GAGTC 3 cut(s) 527, 665, 801
Mox20I TGGCCA 1 cut(s) 1232
Mph1103I ATGCAT 1 cut(s) 448
MscI TGGCCA 1 cut(s) 1232
MseI TTAA 7 cut(s) 96, 210, 239, 732, 738, 921, 1017
MslI CAYNNNNRTG 1 cut(s) 429
Msp20I TGGCCA 1 cut(s) 1232
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 2 cut(s) 118, 1234
MunI CAATTG 2 cut(s) 786, 1124
MvaI CCWGG 2 cut(s) 118, 1234
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 13, 467, 887
NdeI CATATG 1 cut(s) 196
NdeII GATC 6 cut(s) 57, 634, 846, 966, 1011, 1051
NlaIII CATG 3 cut(s) 130, 988, 1165
NlaIV GGNNCC 4 cut(s) 73, 469, 555, 1114
NmuCI GTSAC 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 448
NspI RCATGY 1 cut(s) 988
NspV TTCGAA 1 cut(s) 808
PaqCI CACCTGC 1 cut(s) 1105
PceI AGGCCT 1 cut(s) 315
PciI ACATGT 1 cut(s) 984
PfeI GAWTC 3 cut(s) 563, 805, 1078
PflMI CCANNNNNTGG 1 cut(s) 547
PkrI GCNGC 2 cut(s) 15, 337
PleI GAGTC 3 cut(s) 527, 665, 800
PpsI GAGTC 3 cut(s) 527, 665, 800
PscI ACATGT 1 cut(s) 984
PsiI TTATAA 1 cut(s) 368
Psp1406I AACGTT 1 cut(s) 498
Psp6I CCWGG 2 cut(s) 116, 1232
PspFI CCCAGC 1 cut(s) 373
PspGI CCWGG 2 cut(s) 116, 1232
PspN4I GGNNCC 4 cut(s) 73, 469, 555, 1114
PspPI GGNCC 1 cut(s) 483
RsaI GTAC 2 cut(s) 264, 526
RsaNI GTAC 2 cut(s) 263, 525
RseI CAYNNNNRTG 1 cut(s) 429
SaqAI TTAA 7 cut(s) 96, 210, 239, 732, 738, 921, 1017
SatI GCNGC 2 cut(s) 14, 336
Sau3AI GATC 6 cut(s) 57, 634, 846, 966, 1011, 1051
Sau96I GGNCC 1 cut(s) 483
SchI GAGTC 3 cut(s) 527, 665, 801
ScrFI CCNGG 2 cut(s) 118, 1234
SduI GDGCHC 3 cut(s) 54, 412, 1047
SfaNI GCATC 2 cut(s) 455, 820
SfuI TTCGAA 1 cut(s) 808
SinI GGWCC 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 429
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 7 cut(s) 33, 284, 343, 786, 903, 1031, 1124
SseBI AGGCCT 1 cut(s) 315
SsiI CCGC 5 cut(s) 336, 505, 841, 888, 1136
SspI AATATT 2 cut(s) 100, 747
SspMI CTAG 2 cut(s) 68, 1040
StuI AGGCCT 1 cut(s) 315
StyD4I CCNGG 2 cut(s) 116, 1232
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 4 cut(s) 136, 203, 216, 707
TaiI ACGT 2 cut(s) 248, 501
TaqI TCGA 1 cut(s) 808
TasI AATT 7 cut(s) 33, 284, 343, 786, 903, 1031, 1124
TatI WGTACW 1 cut(s) 262
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 3 cut(s) 563, 805, 1078
Tru1I TTAA 7 cut(s) 96, 210, 239, 732, 738, 921, 1017
Tru9I TTAA 7 cut(s) 96, 210, 239, 732, 738, 921, 1017
TscAI CASTG 5 cut(s) 67, 139, 462, 589, 651
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 3 cut(s) 10, 143, 1118
TspRI CASTG 5 cut(s) 67, 139, 462, 589, 651
Van91I CCANNNNNTGG 1 cut(s) 547
VneI GTGCAC 1 cut(s) 1043
VpaK11BI GGWCC 1 cut(s) 483
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 2 cut(s) 343, 1031
XceI RCATGY 1 cut(s) 988
XmiI GTMKAC 1 cut(s) 1085
XspI CTAG 2 cut(s) 68, 1040
Zsp2I ATGCAT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.