Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 218 |
| AccBSI | CCGCTC | 1 cut(s) | 509 |
| AccI | GTMKAC | 1 cut(s) | 612 |
| AccII | CGCG | 1 cut(s) | 115 |
| AciI | CCGC | 1 cut(s) | 507 |
| AclWI | GGATC | 2 cut(s) | 159, 424 |
| AcoI | YGGCCR | 1 cut(s) | 81 |
| AcsI | RAATTY | 1 cut(s) | 581 |
| AdeI | CACNNNGTG | 1 cut(s) | 302 |
| AfaI | GTAC | 1 cut(s) | 518 |
| AgsI | TTSAA | 2 cut(s) | 318, 529 |
| AjnI | CCWGG | 1 cut(s) | 412 |
| AjuI | GAANNNNNNNTTGG | 1 cut(s) | 653 |
| AluBI | AGCT | 3 cut(s) | 255, 331, 404 |
| AluI | AGCT | 3 cut(s) | 255, 331, 404 |
| Alw21I | GWGCWC | 1 cut(s) | 406 |
| AlwI | GGATC | 2 cut(s) | 159, 424 |
| AoxI | GGCC | 3 cut(s) | 81, 270, 459 |
| ApeKI | GCWGC | 1 cut(s) | 50 |
| ApoI | RAATTY | 1 cut(s) | 581 |
| AspS9I | GGNCC | 1 cut(s) | 271 |
| AsuHPI | GGTGA | 3 cut(s) | 34, 509, 566 |
| AsuNHI | GCTAGC | 1 cut(s) | 182 |
| BanII | GRGCYC | 1 cut(s) | 406 |
| BbsI | GAAGAC | 1 cut(s) | 132 |
| Bbv12I | GWGCWC | 1 cut(s) | 406 |
| BbvI | GCAGC | 1 cut(s) | 37 |
| BccI | CCATC | 1 cut(s) | 479 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 123, 157, 592, 602, 626, 636 |
| BciT130I | CCWGG | 1 cut(s) | 414 |
| BciVI | GTATCC | 1 cut(s) | 477 |
| BfaI | CTAG | 1 cut(s) | 183 |
| BfuI | GTATCC | 1 cut(s) | 477 |
| BisI | GCNGC | 2 cut(s) | 51, 507 |
| BlsI | GCNGC | 2 cut(s) | 52, 508 |
| Bme1390I | CCNGG | 1 cut(s) | 414 |
| BmgT120I | GGNCC | 1 cut(s) | 271 |
| BmiI | GGNNCC | 1 cut(s) | 411 |
| BmrFI | CCNGG | 1 cut(s) | 414 |
| BmsI | GCATC | 4 cut(s) | 217, 302, 570, 654 |
| BmtI | GCTAGC | 1 cut(s) | 186 |
| BpiI | GAAGAC | 1 cut(s) | 132 |
| Bpu10I | CCTNAGC | 1 cut(s) | 405 |
| BpuEI | CTTGAG | 1 cut(s) | 345 |
| BsaBI | GATNNNNATC | 1 cut(s) | 246 |
| BsaJI | CCNNGG | 1 cut(s) | 231 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 619, 649 |
| Bse1I | ACTGG | 1 cut(s) | 187 |
| Bse3DI | GCAATG | 2 cut(s) | 244, 610 |
| Bse8I | GATNNNNATC | 1 cut(s) | 246 |
| BseBI | CCWGG | 1 cut(s) | 414 |
| BseDI | CCNNGG | 1 cut(s) | 231 |
| BseGI | GGATG | 3 cut(s) | 232, 493, 661 |
| BseJI | GATNNNNATC | 1 cut(s) | 246 |
| BseMI | GCAATG | 2 cut(s) | 244, 610 |
| BseMII | CTCAG | 3 cut(s) | 265, 350, 419 |
| BseNI | ACTGG | 1 cut(s) | 187 |
| BseRI | GAGGAG | 1 cut(s) | 627 |
| BseX3I | CGGCCG | 1 cut(s) | 81 |
| BseXI | GCAGC | 1 cut(s) | 37 |
| Bsh1236I | CGCG | 1 cut(s) | 115 |
| Bsh1285I | CGRYCG | 2 cut(s) | 84, 616 |
| BshFI | GGCC | 3 cut(s) | 83, 272, 461 |
| BsiEI | CGRYCG | 2 cut(s) | 84, 616 |
| BsiHKAI | GWGCWC | 1 cut(s) | 406 |
| BsmI | GAATGC | 1 cut(s) | 380 |
| BsnI | GGCC | 3 cut(s) | 83, 272, 461 |
| Bsp1286I | GDGCHC | 1 cut(s) | 406 |
| Bsp1407I | TGTACA | 1 cut(s) | 516 |
| Bsp143I | GATC | 4 cut(s) | 126, 164, 247, 416 |
| BspACI | CCGC | 1 cut(s) | 507 |
| BspANI | GGCC | 3 cut(s) | 83, 272, 461 |
| BspCNI | CTCAG | 3 cut(s) | 264, 349, 418 |
| BspFNI | CGCG | 1 cut(s) | 115 |
| BspLI | GGNNCC | 1 cut(s) | 411 |
| BspOI | GCTAGC | 1 cut(s) | 186 |
| BspPI | GGATC | 2 cut(s) | 159, 424 |
| BsrBI | CCGCTC | 1 cut(s) | 509 |
| BsrDI | GCAATG | 2 cut(s) | 244, 610 |
| BsrGI | TGTACA | 1 cut(s) | 516 |
| BsrI | ACTGG | 1 cut(s) | 187 |
| BssECI | CCNNGG | 1 cut(s) | 231 |
| BssMI | GATC | 4 cut(s) | 126, 164, 247, 416 |
| BssT1I | CCWWGG | 1 cut(s) | 231 |
| Bst2UI | CCWGG | 1 cut(s) | 414 |
| Bst4CI | ACNGT | 4 cut(s) | 46, 220, 617, 669 |
| Bst6I | CTCTTC | 1 cut(s) | 32 |
| BstAUI | TGTACA | 1 cut(s) | 516 |
| BstC8I | GCNNGC | 3 cut(s) | 184, 333, 445 |
| BstDEI | CTNAG | 4 cut(s) | 251, 336, 405, 641 |
| BstF5I | GGATG | 3 cut(s) | 232, 493, 661 |
| BstFNI | CGCG | 1 cut(s) | 115 |
| BstKTI | GATC | 4 cut(s) | 129, 167, 250, 419 |
| BstMBI | GATC | 4 cut(s) | 126, 164, 247, 416 |
| BstMCI | CGRYCG | 2 cut(s) | 84, 616 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 112 |
| BstNI | CCWGG | 1 cut(s) | 414 |
| BstSCI | CCNGG | 1 cut(s) | 412 |
| BstUI | CGCG | 1 cut(s) | 115 |
| BstV1I | GCAGC | 1 cut(s) | 37 |
| BstV2I | GAAGAC | 1 cut(s) | 132 |
| BstX2I | RGATCY | 1 cut(s) | 416 |
| BstYI | RGATCY | 1 cut(s) | 416 |
| BstZI | CGGCCG | 1 cut(s) | 81 |
| BsuI | GTATCC | 1 cut(s) | 477 |
| BsuRI | GGCC | 3 cut(s) | 83, 272, 461 |
| BtsCI | GGATG | 3 cut(s) | 232, 493, 661 |
| BtsIMutI | CAGTG | 1 cut(s) | 216 |
| Cac8I | GCNNGC | 3 cut(s) | 184, 333, 445 |
| Cfr13I | GGNCC | 1 cut(s) | 271 |
| Csp6I | GTAC | 1 cut(s) | 517 |
| CviAII | CATG | 1 cut(s) | 681 |
| CviQI | GTAC | 1 cut(s) | 517 |
| DdeI | CTNAG | 4 cut(s) | 251, 336, 405, 641 |
| DpnI | GATC | 4 cut(s) | 128, 166, 249, 418 |
| DpnII | GATC | 4 cut(s) | 126, 164, 247, 416 |
| DraIII | CACNNNGTG | 1 cut(s) | 302 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 218 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 218 |
| EaeI | YGGCCR | 1 cut(s) | 81 |
| EagI | CGGCCG | 1 cut(s) | 81 |
| Eam1104I | CTCTTC | 1 cut(s) | 32 |
| EarI | CTCTTC | 1 cut(s) | 32 |
| Ecl136II | GAGCTC | 1 cut(s) | 404 |
| EclXI | CGGCCG | 1 cut(s) | 81 |
| Eco130I | CCWWGG | 1 cut(s) | 231 |
| Eco24I | GRGCYC | 1 cut(s) | 406 |
| Eco52I | CGGCCG | 1 cut(s) | 81 |
| Eco53kI | GAGCTC | 1 cut(s) | 404 |
| EcoICRI | GAGCTC | 1 cut(s) | 404 |
| EcoO109I | RGGNCCY | 1 cut(s) | 271 |
| EcoRII | CCWGG | 1 cut(s) | 412 |
| EcoT14I | CCWWGG | 1 cut(s) | 231 |
| EcoT38I | GRGCYC | 1 cut(s) | 406 |
| ErhI | CCWWGG | 1 cut(s) | 231 |
| FaeI | CATG | 1 cut(s) | 684 |
| FaiI | YATR | 4 cut(s) | 96, 345, 621, 682 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 557, 589 |
| FatI | CATG | 1 cut(s) | 680 |
| FblI | GTMKAC | 1 cut(s) | 612 |
| Fnu4HI | GCNGC | 2 cut(s) | 51, 507 |
| FokI | GGATG | 3 cut(s) | 239, 500, 668 |
| FriOI | GRGCYC | 1 cut(s) | 406 |
| Fsp4HI | GCNGC | 2 cut(s) | 51, 507 |
| FspBI | CTAG | 1 cut(s) | 183 |
| GluI | GCNGC | 2 cut(s) | 51, 507 |
| HaeIII | GGCC | 3 cut(s) | 83, 272, 461 |
| Hin1II | CATG | 1 cut(s) | 684 |
| HincII | GTYRAC | 2 cut(s) | 592, 613 |
| HindII | GTYRAC | 2 cut(s) | 592, 613 |
| HinfI | GANTC | 3 cut(s) | 86, 137, 546 |
| HphI | GGTGA | 3 cut(s) | 34, 509, 566 |
| Hpy166II | GTNNAC | 5 cut(s) | 192, 208, 545, 592, 613 |
| Hpy188I | TCNGA | 1 cut(s) | 32 |
| Hpy188III | TCNNGA | 3 cut(s) | 407, 526, 649 |
| Hpy8I | GTNNAC | 5 cut(s) | 192, 208, 545, 592, 613 |
| Hpy99I | CGWCG | 1 cut(s) | 50 |
| HpyAV | CCTTC | 2 cut(s) | 593, 646 |
| HpyCH4III | ACNGT | 4 cut(s) | 46, 220, 617, 669 |
| HpyCH4V | TGCA | 3 cut(s) | 390, 447, 680 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 112 |
| HpyF3I | CTNAG | 4 cut(s) | 251, 336, 405, 641 |
| Hsp92II | CATG | 1 cut(s) | 684 |
| Kzo9I | GATC | 4 cut(s) | 126, 164, 247, 416 |
| LmnI | GCTCC | 4 cut(s) | 260, 401, 409, 514 |
| LpnPI | CCDG | 5 cut(s) | 200, 317, 392, 399, 426 |
| Lsp1109I | GCAGC | 1 cut(s) | 37 |
| LweI | GCATC | 4 cut(s) | 217, 302, 570, 654 |
| MaeI | CTAG | 1 cut(s) | 183 |
| MaeIII | GTNAC | 2 cut(s) | 497, 554 |
| MalI | GATC | 4 cut(s) | 128, 166, 249, 418 |
| MbiI | CCGCTC | 1 cut(s) | 509 |
| MboI | GATC | 4 cut(s) | 126, 164, 247, 416 |
| MboII | GAAGA | 6 cut(s) | 3, 19, 45, 102, 132, 673 |
| MflI | RGATCY | 1 cut(s) | 416 |
| MhlI | GDGCHC | 1 cut(s) | 406 |
| MluCI | AATT | 2 cut(s) | 313, 581 |
| MlyI | GAGTC | 2 cut(s) | 146, 540 |
| MmeI | TCCRAC | 1 cut(s) | 114 |
| MnlI | CCTC | 2 cut(s) | 133, 648 |
| MseI | TTAA | 1 cut(s) | 456 |
| MslI | CAYNNNNRTG | 1 cut(s) | 298 |
| MspR9I | CCNGG | 1 cut(s) | 414 |
| Mva1269I | GAATGC | 1 cut(s) | 380 |
| MvaI | CCWGG | 1 cut(s) | 414 |
| MvnI | CGCG | 1 cut(s) | 115 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 112 |
| NdeII | GATC | 4 cut(s) | 126, 164, 247, 416 |
| NheI | GCTAGC | 1 cut(s) | 182 |
| NlaIII | CATG | 1 cut(s) | 684 |
| NlaIV | GGNNCC | 1 cut(s) | 411 |
| NmeAIII | GCCGAG | 2 cut(s) | 59, 487 |
| NmuCI | GTSAC | 2 cut(s) | 497, 554 |
| PctI | GAATGC | 1 cut(s) | 380 |
| PfeI | GAWTC | 1 cut(s) | 86 |
| PkrI | GCNGC | 2 cut(s) | 52, 508 |
| PleI | GAGTC | 2 cut(s) | 145, 540 |
| PpsI | GAGTC | 2 cut(s) | 145, 540 |
| Psp124BI | GAGCTC | 1 cut(s) | 406 |
| Psp6I | CCWGG | 1 cut(s) | 412 |
| PspGI | CCWGG | 1 cut(s) | 412 |
| PspN4I | GGNNCC | 1 cut(s) | 411 |
| PspPI | GGNCC | 1 cut(s) | 271 |
| PsuI | RGATCY | 1 cut(s) | 416 |
| RsaI | GTAC | 1 cut(s) | 518 |
| RsaNI | GTAC | 1 cut(s) | 517 |
| RseI | CAYNNNNRTG | 1 cut(s) | 298 |
| SacI | GAGCTC | 1 cut(s) | 406 |
| SalI | GTCGAC | 1 cut(s) | 611 |
| SaqAI | TTAA | 1 cut(s) | 456 |
| SatI | GCNGC | 2 cut(s) | 51, 507 |
| Sau3AI | GATC | 4 cut(s) | 126, 164, 247, 416 |
| Sau96I | GGNCC | 1 cut(s) | 271 |
| SchI | GAGTC | 2 cut(s) | 146, 540 |
| ScrFI | CCNGG | 1 cut(s) | 414 |
| SduI | GDGCHC | 1 cut(s) | 406 |
| SetI | ASST | 6 cut(s) | 257, 333, 367, 406, 486, 544 |
| SfaNI | GCATC | 4 cut(s) | 217, 302, 570, 654 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 298 |
| SmlI | CTYRAG | 1 cut(s) | 360 |
| SmoI | CTYRAG | 1 cut(s) | 360 |
| Sse9I | AATT | 2 cut(s) | 313, 581 |
| SsiI | CCGC | 1 cut(s) | 507 |
| SspMI | CTAG | 1 cut(s) | 183 |
| SstI | GAGCTC | 1 cut(s) | 406 |
| StyD4I | CCNGG | 1 cut(s) | 412 |
| StyI | CCWWGG | 1 cut(s) | 231 |
| TaaI | ACNGT | 4 cut(s) | 46, 220, 617, 669 |
| TaqI | TCGA | 6 cut(s) | 125, 143, 163, 533, 612, 648 |
| TasI | AATT | 2 cut(s) | 313, 581 |
| TatI | WGTACW | 1 cut(s) | 516 |
| TauI | GCSGC | 1 cut(s) | 509 |
| TfiI | GAWTC | 1 cut(s) | 86 |
| Tru1I | TTAA | 1 cut(s) | 456 |
| Tru9I | TTAA | 1 cut(s) | 456 |
| TscAI | CASTG | 1 cut(s) | 223 |
| TseFI | GTSAC | 2 cut(s) | 497, 554 |
| TseI | GCWGC | 1 cut(s) | 50 |
| Tsp45I | GTSAC | 2 cut(s) | 497, 554 |
| TspGWI | ACGGA | 1 cut(s) | 144 |
| TspRI | CASTG | 1 cut(s) | 223 |
| XapI | RAATTY | 1 cut(s) | 581 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 175 |
| XmiI | GTMKAC | 1 cut(s) | 612 |
| XspI | CTAG | 1 cut(s) | 183 |