Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 713 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 79, 456 |
| AccI | GTMKAC | 1 cut(s) | 976 |
| AciI | CCGC | 3 cut(s) | 328, 466, 1013 |
| AcoI | YGGCCR | 1 cut(s) | 1047 |
| AcsI | RAATTY | 1 cut(s) | 346 |
| AcuI | CTGAAG | 1 cut(s) | 776 |
| AdeI | CACNNNGTG | 1 cut(s) | 986 |
| AfaI | GTAC | 4 cut(s) | 57, 511, 855, 1437 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 79, 456, 778, 1138, 1347, 1426 |
| AflIII | ACRYGT | 1 cut(s) | 1143 |
| AgsI | TTSAA | 6 cut(s) | 265, 503, 544, 1150, 1312, 1442 |
| AjiI | CACGTC | 1 cut(s) | 524 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 65, 1103 |
| Ama87I | CYCGRG | 1 cut(s) | 659 |
| AoxI | GGCC | 3 cut(s) | 278, 820, 1047 |
| ApeKI | GCWGC | 8 cut(s) | 322, 920, 989, 992, 1097, 1199, 1373, 1376 |
| ApoI | RAATTY | 1 cut(s) | 346 |
| AspS9I | GGNCC | 4 cut(s) | 41, 279, 1125, 1236 |
| AsuHPI | GGTGA | 4 cut(s) | 383, 1002, 1031, 1178 |
| AsuII | TTCGAA | 1 cut(s) | 17 |
| AvaI | CYCGRG | 1 cut(s) | 659 |
| AvaII | GGWCC | 3 cut(s) | 41, 1125, 1236 |
| BaeGI | GKGCMC | 1 cut(s) | 529 |
| BanI | GGYRCC | 1 cut(s) | 713 |
| BanII | GRGCYC | 1 cut(s) | 72 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 39, 71 |
| BbvI | GCAGC | 8 cut(s) | 334, 907, 1001, 1004, 1109, 1186, 1360, 1388 |
| BccI | CCATC | 4 cut(s) | 614, 964, 1030, 1058 |
| BceAI | ACGGC | 2 cut(s) | 1062, 1251 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 527, 561 |
| BfaI | CTAG | 2 cut(s) | 582, 1002 |
| BfmI | CTRYAG | 3 cut(s) | 631, 687, 1374 |
| BglII | AGATCT | 1 cut(s) | 1059 |
| BisI | GCNGC | 8 cut(s) | 323, 921, 990, 993, 1098, 1200, 1374, 1377 |
| BlsI | GCNGC | 8 cut(s) | 324, 922, 991, 994, 1099, 1201, 1375, 1378 |
| Bme18I | GGWCC | 3 cut(s) | 41, 1125, 1236 |
| BmeT110I | CYCGRG | 1 cut(s) | 659 |
| BmgBI | CACGTC | 1 cut(s) | 524 |
| BmgT120I | GGNCC | 4 cut(s) | 41, 279, 1125, 1236 |
| BmiI | GGNNCC | 4 cut(s) | 715, 930, 954, 1237 |
| BmsI | GCATC | 2 cut(s) | 1306, 1367 |
| BpmI | CTGGAG | 1 cut(s) | 920 |
| Bpu10I | CCTNAGC | 1 cut(s) | 66 |
| Bpu14I | TTCGAA | 1 cut(s) | 17 |
| BsaJI | CCNNGG | 1 cut(s) | 1239 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 79, 456, 778, 1138, 1347, 1426 |
| Bse1I | ACTGG | 2 cut(s) | 59, 513 |
| Bse3DI | GCAATG | 2 cut(s) | 468, 1353 |
| BseDI | CCNNGG | 1 cut(s) | 1239 |
| BseGI | GGATG | 6 cut(s) | 333, 1041, 1050, 1090, 1358, 1405 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 79, 456, 778, 1138, 1347, 1426 |
| BseMI | GCAATG | 2 cut(s) | 468, 1353 |
| BseMII | CTCAG | 7 cut(s) | 57, 748, 837, 939, 1095, 1232, 1435 |
| BseNI | ACTGG | 2 cut(s) | 59, 513 |
| BseRI | GAGGAG | 1 cut(s) | 645 |
| BseSI | GKGCMC | 1 cut(s) | 529 |
| BseXI | GCAGC | 8 cut(s) | 334, 907, 1001, 1004, 1109, 1186, 1360, 1388 |
| BsgI | GTGCAG | 1 cut(s) | 1008 |
| BshFI | GGCC | 3 cut(s) | 280, 822, 1049 |
| BshNI | GGYRCC | 1 cut(s) | 713 |
| BsiHKCI | CYCGRG | 1 cut(s) | 659 |
| BslFI | GGGAC | 2 cut(s) | 794, 1249 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 79, 456, 778, 1138, 1347, 1426 |
| BsmFI | GGGAC | 2 cut(s) | 794, 1249 |
| BsmI | GAATGC | 2 cut(s) | 550, 1459 |
| BsnI | GGCC | 3 cut(s) | 280, 822, 1049 |
| BsoBI | CYCGRG | 1 cut(s) | 659 |
| Bsp119I | TTCGAA | 1 cut(s) | 17 |
| Bsp1286I | GDGCHC | 2 cut(s) | 72, 529 |
| Bsp143I | GATC | 2 cut(s) | 269, 1059 |
| Bsp19I | CCATGG | 1 cut(s) | 1239 |
| BspACI | CCGC | 3 cut(s) | 328, 466, 1013 |
| BspANI | GGCC | 3 cut(s) | 280, 822, 1049 |
| BspCNI | CTCAG | 7 cut(s) | 58, 747, 838, 940, 1096, 1231, 1434 |
| BspHI | TCATGA | 2 cut(s) | 121, 1284 |
| BspLI | GGNNCC | 4 cut(s) | 715, 930, 954, 1237 |
| BspMAI | CTGCAG | 2 cut(s) | 691, 1378 |
| BspT104I | TTCGAA | 1 cut(s) | 17 |
| BspT107I | GGYRCC | 1 cut(s) | 713 |
| BsrDI | GCAATG | 2 cut(s) | 468, 1353 |
| BsrI | ACTGG | 2 cut(s) | 59, 513 |
| BssECI | CCNNGG | 1 cut(s) | 1239 |
| BssMI | GATC | 2 cut(s) | 269, 1059 |
| BssNAI | GTATAC | 1 cut(s) | 977 |
| BssT1I | CCWWGG | 1 cut(s) | 1239 |
| Bst1107I | GTATAC | 1 cut(s) | 977 |
| Bst4CI | ACNGT | 3 cut(s) | 79, 614, 1255 |
| BstBI | TTCGAA | 1 cut(s) | 17 |
| BstC8I | GCNNGC | 3 cut(s) | 114, 1264, 1371 |
| BstDSI | CCRYGG | 1 cut(s) | 1239 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1424 |
| BstF5I | GGATG | 6 cut(s) | 333, 1041, 1050, 1090, 1358, 1405 |
| BstKTI | GATC | 2 cut(s) | 272, 1062 |
| BstMBI | GATC | 2 cut(s) | 269, 1059 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 363, 1325, 1415 |
| BstSFI | CTRYAG | 3 cut(s) | 631, 687, 1374 |
| BstSLI | GKGCMC | 1 cut(s) | 529 |
| BstV1I | GCAGC | 8 cut(s) | 334, 907, 1001, 1004, 1109, 1186, 1360, 1388 |
| BstX2I | RGATCY | 1 cut(s) | 1059 |
| BstYI | RGATCY | 1 cut(s) | 1059 |
| BstZ17I | GTATAC | 1 cut(s) | 977 |
| BsuRI | GGCC | 3 cut(s) | 280, 822, 1049 |
| BtgI | CCRYGG | 1 cut(s) | 1239 |
| BtrI | CACGTC | 1 cut(s) | 524 |
| BtsCI | GGATG | 6 cut(s) | 333, 1041, 1050, 1090, 1358, 1405 |
| BtsIMutI | CAGTG | 2 cut(s) | 742, 945 |
| Cac8I | GCNNGC | 3 cut(s) | 114, 1264, 1371 |
| CaiI | CAGNNNCTG | 2 cut(s) | 65, 1103 |
| CciI | TCATGA | 2 cut(s) | 121, 1284 |
| Cfr13I | GGNCC | 4 cut(s) | 41, 279, 1125, 1236 |
| CseI | GACGC | 2 cut(s) | 884, 1308 |
| Csp6I | GTAC | 4 cut(s) | 56, 510, 854, 1436 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 297, 332 |
| CviQI | GTAC | 4 cut(s) | 56, 510, 854, 1436 |
| DpnI | GATC | 2 cut(s) | 271, 1061 |
| DpnII | GATC | 2 cut(s) | 269, 1059 |
| DraIII | CACNNNGTG | 1 cut(s) | 986 |
| EaeI | YGGCCR | 1 cut(s) | 1047 |
| Eco130I | CCWWGG | 1 cut(s) | 1239 |
| Eco24I | GRGCYC | 1 cut(s) | 72 |
| Eco47I | GGWCC | 3 cut(s) | 41, 1125, 1236 |
| Eco57I | CTGAAG | 1 cut(s) | 776 |
| Eco88I | CYCGRG | 1 cut(s) | 659 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1424 |
| EcoT14I | CCWWGG | 1 cut(s) | 1239 |
| EcoT38I | GRGCYC | 1 cut(s) | 72 |
| ErhI | CCWWGG | 1 cut(s) | 1239 |
| FaqI | GGGAC | 2 cut(s) | 794, 1249 |
| FblI | GTMKAC | 1 cut(s) | 976 |
| Fnu4HI | GCNGC | 8 cut(s) | 323, 921, 990, 993, 1098, 1200, 1374, 1377 |
| FokI | GGATG | 6 cut(s) | 320, 1037, 1048, 1097, 1345, 1412 |
| FriOI | GRGCYC | 1 cut(s) | 72 |
| Fsp4HI | GCNGC | 8 cut(s) | 323, 921, 990, 993, 1098, 1200, 1374, 1377 |
| FspBI | CTAG | 2 cut(s) | 582, 1002 |
| GluI | GCNGC | 8 cut(s) | 323, 921, 990, 993, 1098, 1200, 1374, 1377 |
| GsuI | CTGGAG | 1 cut(s) | 920 |
| HaeIII | GGCC | 3 cut(s) | 280, 822, 1049 |
| HgaI | GACGC | 2 cut(s) | 884, 1308 |
| HincII | GTYRAC | 1 cut(s) | 801 |
| HindII | GTYRAC | 1 cut(s) | 801 |
| HindIII | AAGCTT | 2 cut(s) | 227, 1023 |
| HinfI | GANTC | 4 cut(s) | 261, 284, 787, 806 |
| HpaI | GTTAAC | 1 cut(s) | 801 |
| HphI | GGTGA | 4 cut(s) | 383, 1002, 1031, 1178 |
| Hpy166II | GTNNAC | 4 cut(s) | 217, 801, 977, 1125 |
| Hpy188I | TCNGA | 6 cut(s) | 187, 338, 795, 1196, 1251, 1424 |
| Hpy8I | GTNNAC | 4 cut(s) | 217, 801, 977, 1125 |
| HpyAV | CCTTC | 9 cut(s) | 86, 237, 362, 740, 812, 934, 966, 1085, 1389 |
| HpyCH4III | ACNGT | 3 cut(s) | 79, 614, 1255 |
| HpyCH4IV | ACGT | 3 cut(s) | 523, 856, 1145 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 363, 1325, 1415 |
| HpySE526I | ACGT | 3 cut(s) | 523, 856, 1145 |
| KspAI | GTTAAC | 1 cut(s) | 801 |
| Kzo9I | GATC | 2 cut(s) | 269, 1059 |
| LmnI | GCTCC | 2 cut(s) | 330, 1094 |
| Lsp1109I | GCAGC | 8 cut(s) | 334, 907, 1001, 1004, 1109, 1186, 1360, 1388 |
| LweI | GCATC | 2 cut(s) | 1306, 1367 |
| MaeI | CTAG | 2 cut(s) | 582, 1002 |
| MaeII | ACGT | 3 cut(s) | 523, 856, 1145 |
| MaeIII | GTNAC | 4 cut(s) | 381, 468, 682, 1320 |
| MalI | GATC | 2 cut(s) | 271, 1061 |
| MboI | GATC | 2 cut(s) | 269, 1059 |
| MboII | GAAGA | 4 cut(s) | 59, 314, 782, 1168 |
| MfeI | CAATTG | 2 cut(s) | 132, 1203 |
| MflI | RGATCY | 1 cut(s) | 1059 |
| MhlI | GDGCHC | 2 cut(s) | 72, 529 |
| MluCI | AATT | 5 cut(s) | 132, 293, 346, 824, 1203 |
| MmeI | TCCRAC | 1 cut(s) | 1274 |
| MseI | TTAA | 1 cut(s) | 800 |
| MunI | CAATTG | 2 cut(s) | 132, 1203 |
| Mva1269I | GAATGC | 2 cut(s) | 550, 1459 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 363, 1325, 1415 |
| NcoI | CCATGG | 1 cut(s) | 1239 |
| NdeII | GATC | 2 cut(s) | 269, 1059 |
| NlaIV | GGNNCC | 4 cut(s) | 715, 930, 954, 1237 |
| NmuCI | GTSAC | 1 cut(s) | 1320 |
| NspV | TTCGAA | 1 cut(s) | 17 |
| PagI | TCATGA | 2 cut(s) | 121, 1284 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1151 |
| PctI | GAATGC | 2 cut(s) | 550, 1459 |
| PfeI | GAWTC | 4 cut(s) | 261, 284, 787, 806 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 79, 456 |
| PkrI | GCNGC | 8 cut(s) | 324, 922, 991, 994, 1099, 1201, 1375, 1378 |
| PspN4I | GGNNCC | 4 cut(s) | 715, 930, 954, 1237 |
| PspPI | GGNCC | 4 cut(s) | 41, 279, 1125, 1236 |
| PstI | CTGCAG | 2 cut(s) | 691, 1378 |
| PstNI | CAGNNNCTG | 2 cut(s) | 65, 1103 |
| PsuI | RGATCY | 1 cut(s) | 1059 |
| RsaI | GTAC | 4 cut(s) | 57, 511, 855, 1437 |
| RsaNI | GTAC | 4 cut(s) | 56, 510, 854, 1436 |
| SaqAI | TTAA | 1 cut(s) | 800 |
| SatI | GCNGC | 8 cut(s) | 323, 921, 990, 993, 1098, 1200, 1374, 1377 |
| Sau3AI | GATC | 2 cut(s) | 269, 1059 |
| Sau96I | GGNCC | 4 cut(s) | 41, 279, 1125, 1236 |
| SduI | GDGCHC | 2 cut(s) | 72, 529 |
| SfaNI | GCATC | 2 cut(s) | 1306, 1367 |
| SfcI | CTRYAG | 3 cut(s) | 631, 687, 1374 |
| SfuI | TTCGAA | 1 cut(s) | 17 |
| SinI | GGWCC | 3 cut(s) | 41, 1125, 1236 |
| Sse9I | AATT | 5 cut(s) | 132, 293, 346, 824, 1203 |
| SsiI | CCGC | 3 cut(s) | 328, 466, 1013 |
| SspI | AATATT | 1 cut(s) | 506 |
| SspMI | CTAG | 2 cut(s) | 582, 1002 |
| StyI | CCWWGG | 1 cut(s) | 1239 |
| TaaI | ACNGT | 3 cut(s) | 79, 614, 1255 |
| TaiI | ACGT | 3 cut(s) | 526, 859, 1148 |
| TaqI | TCGA | 3 cut(s) | 17, 768, 1154 |
| TasI | AATT | 5 cut(s) | 132, 293, 346, 824, 1203 |
| TatI | WGTACW | 1 cut(s) | 1435 |
| TfiI | GAWTC | 4 cut(s) | 261, 284, 787, 806 |
| Tru1I | TTAA | 1 cut(s) | 800 |
| Tru9I | TTAA | 1 cut(s) | 800 |
| TscAI | CASTG | 2 cut(s) | 742, 952 |
| TseFI | GTSAC | 1 cut(s) | 1320 |
| TseI | GCWGC | 8 cut(s) | 322, 920, 989, 992, 1097, 1199, 1373, 1376 |
| Tsp45I | GTSAC | 1 cut(s) | 1320 |
| TspGWI | ACGGA | 1 cut(s) | 1169 |
| TspRI | CASTG | 2 cut(s) | 742, 952 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 79, 456 |
| VpaK11BI | GGWCC | 3 cut(s) | 41, 1125, 1236 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1424 |
| XapI | RAATTY | 1 cut(s) | 346 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 412 |
| XmiI | GTMKAC | 1 cut(s) | 976 |
| XspI | CTAG | 2 cut(s) | 582, 1002 |