MD12G1087900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001089
Physical Location & Seq
Forward (+)
10815275 .. 10816036
762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1087900.v1.1.491

Sequence Viewer

Length: 762 bp
ATGGCTTCGTCATCTTTGATGGAGATTCAAGTACCAAAGCATGCAAAAGGAGAGCGCTCAGCCAAGGTTCCCTTACTTCCCGTTTCACACAGCCCTAATTCAAAATCCCATATTTCGACAAGGTCTACCAATGTCTCCACTACACCAACCATTGCCAAATTCACCTTCCTCATTTTTCTCTCCTTGATCACTCTTGCCATCATCGTATTTCTACCGTTAATACGCAAGGAGCTCACTCTAGGCCCCCAAGCCCCCGTTGTCCAACTTACTGCCTTATCCGTGCACAAATTCAACGTCTCGGCCAGAAACCTAACAGCCGAATGGGACGTCATTTTGAAAATTGCTAACCCTAATCTTCTCTCCTCTGTTTGGTTCAACCGGCTTGAAGGTTTCGTTCTGTATGAGGACAGACCGCTTGCCATCGAAACAATGGAGCCATTCGGTCTGCCCATGAAGACAAAAACAGAACTGCATCTCAGGCTTAGAATGGCGAATTGGGAAGGGGATCAACCAGCGCTTAAGCAAGGGATGTTGAATAAGATGACGAAAGATAGAGAACTGGGGTGTGTGAGATTTAGTGTTCAGATGGCGGTCTGGGCAACGTATAGGAGTGGTTGGTGGTGGTCGGCGCAACATGTGATTATGAATCCTCAGTGTTTAGATTTGCAGATTGGGTTTGTGCCAGGCGCCACAGCTATAGGTTTTGGAATATTGATGGGTGATGTACCCAGGACGTGCTATGTTCCCATGCTAGCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

254

Amino Acids

28.28

Weight (kDa)

9.48

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 330
AccB1I GGYRCC 1 cut(s) 686
AccI GTMKAC 1 cut(s) 125
AciI CCGC 2 cut(s) 413, 590
AclWI GGATC 1 cut(s) 513
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 2 cut(s) 158, 287
AcyI GRCGYC 2 cut(s) 327, 687
AfaI GTAC 2 cut(s) 33, 726
AfeI AGCGCT 2 cut(s) 56, 516
AfiI CCNNNNNNNGG 1 cut(s) 369
AflII CTTAAG 1 cut(s) 518
AflIII ACRYGT 1 cut(s) 634
AgsI TTSAA 7 cut(s) 29, 102, 292, 337, 376, 386, 535
AjiI CACGTC 1 cut(s) 735
AjnI CCWGG 2 cut(s) 682, 728
AjuI GAANNNNNNNTTGG 4 cut(s) 149, 181, 478, 510
AluBI AGCT 3 cut(s) 232, 695, 755
AluI AGCT 3 cut(s) 232, 695, 755
Alw21I GWGCWC 2 cut(s) 234, 285
Alw26I GTCTC 2 cut(s) 139, 301
Alw44I GTGCAC 1 cut(s) 281
AlwI GGATC 1 cut(s) 513
Aor51HI AGCGCT 2 cut(s) 56, 516
AoxI GGCC 2 cut(s) 241, 300
ApaLI GTGCAC 1 cut(s) 281
ApoI RAATTY 2 cut(s) 158, 287
ArsI GACNNNNNNTTYG 4 cut(s) 279, 311, 312, 344
AspLEI GCGC 4 cut(s) 57, 517, 631, 689
AspS9I GGNCC 1 cut(s) 242
AsuHPI GGTGA 2 cut(s) 154, 731
AsuNHI GCTAGC 1 cut(s) 751
BaeGI GKGCMC 1 cut(s) 285
BanI GGYRCC 1 cut(s) 686
BanII GRGCYC 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 461
Bbv12I GWGCWC 2 cut(s) 234, 285
BccI CCATC 5 cut(s) 13, 206, 428, 580, 709
BciT130I CCWGG 2 cut(s) 684, 730
BclI TGATCA 1 cut(s) 186
BcoDI GTCTC 2 cut(s) 139, 301
BfaI CTAG 2 cut(s) 239, 752
BfmI CTRYAG 1 cut(s) 696
BfoI RGCGCY 3 cut(s) 58, 518, 690
BfrI CTTAAG 1 cut(s) 518
BlpI GCTNAGC 1 cut(s) 58
Bme1390I CCNGG 2 cut(s) 684, 730
BmgBI CACGTC 1 cut(s) 735
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 4 cut(s) 69, 244, 435, 688
BmrFI CCNGG 2 cut(s) 684, 730
BmrI ACTGGG 1 cut(s) 569
BmsI GCATC 1 cut(s) 481
BmtI GCTAGC 1 cut(s) 755
BmuI ACTGGG 1 cut(s) 569
BpiI GAAGAC 1 cut(s) 461
Bpu1102I GCTNAGC 1 cut(s) 58
BsaHI GRCGYC 2 cut(s) 327, 687
BsaJI CCNNGG 2 cut(s) 63, 728
Bsc4I CCNNNNNNNGG 1 cut(s) 369
Bse118I RCCGGY 1 cut(s) 378
Bse1I ACTGG 1 cut(s) 564
Bse3DI GCAATG 1 cut(s) 150
BseBI CCWGG 2 cut(s) 684, 730
BseDI CCNNGG 2 cut(s) 63, 728
BseGI GGATG 1 cut(s) 534
BseLI CCNNNNNNNGG 1 cut(s) 369
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 4 cut(s) 72, 490, 665, 747
BseNI ACTGG 1 cut(s) 564
BseRI GAGGAG 1 cut(s) 352
BseSI GKGCMC 1 cut(s) 285
BshFI GGCC 2 cut(s) 243, 302
BshNI GGYRCC 1 cut(s) 686
BsiHKAI GWGCWC 2 cut(s) 234, 285
BsiSI CCGG 1 cut(s) 379
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 1 cut(s) 369
BsmAI GTCTC 2 cut(s) 139, 301
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 2 cut(s) 243, 302
Bsp1286I GDGCHC 2 cut(s) 234, 285
Bsp143I GATC 2 cut(s) 186, 505
Bsp1720I GCTNAGC 1 cut(s) 58
BspACI CCGC 2 cut(s) 413, 590
BspANI GGCC 2 cut(s) 243, 302
BspCNI CTCAG 4 cut(s) 71, 489, 664, 748
BspLI GGNNCC 4 cut(s) 69, 244, 435, 688
BspOI GCTAGC 1 cut(s) 755
BspPI GGATC 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 686
BspTI CTTAAG 1 cut(s) 518
BsrDI GCAATG 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 378
BsrI ACTGG 1 cut(s) 564
BssAI RCCGGY 1 cut(s) 378
BssECI CCNNGG 2 cut(s) 63, 728
BssMI GATC 2 cut(s) 186, 505
BssNI GRCGYC 2 cut(s) 327, 687
BssT1I CCWWGG 1 cut(s) 63
Bst2UI CCWGG 2 cut(s) 684, 730
Bst4CI ACNGT 1 cut(s) 216
BstACI GRCGYC 2 cut(s) 327, 687
BstAFI CTTAAG 1 cut(s) 518
BstC8I GCNNGC 3 cut(s) 42, 417, 753
BstDEI CTNAG 5 cut(s) 58, 476, 482, 651, 756
BstF5I GGATG 1 cut(s) 534
BstH2I RGCGCY 3 cut(s) 58, 518, 690
BstHHI GCGC 4 cut(s) 57, 517, 631, 689
BstKTI GATC 2 cut(s) 189, 508
BstMAI GTCTC 2 cut(s) 139, 301
BstMBI GATC 2 cut(s) 186, 505
BstMWI GCNNNNNNNGC 2 cut(s) 478, 596
BstNI CCWGG 2 cut(s) 684, 730
BstNSI RCATGY 2 cut(s) 44, 638
BstSCI CCNGG 2 cut(s) 682, 728
BstSFI CTRYAG 1 cut(s) 696
BstSLI GKGCMC 1 cut(s) 285
BstV2I GAAGAC 1 cut(s) 461
BsuRI GGCC 2 cut(s) 243, 302
BtrI CACGTC 1 cut(s) 735
BtsCI GGATG 1 cut(s) 534
BtsIMutI CAGTG 1 cut(s) 659
Cac8I GCNNGC 3 cut(s) 42, 417, 753
CfoI GCGC 4 cut(s) 57, 517, 631, 689
Cfr10I RCCGGY 1 cut(s) 378
Cfr13I GGNCC 1 cut(s) 242
Csp6I GTAC 2 cut(s) 32, 725
CviAII CATG 4 cut(s) 41, 451, 635, 748
CviQI GTAC 2 cut(s) 32, 725
DdeI CTNAG 5 cut(s) 58, 476, 482, 651, 756
DinI GGCGCC 1 cut(s) 688
DpnI GATC 2 cut(s) 188, 507
DpnII GATC 2 cut(s) 186, 505
EaeI YGGCCR 1 cut(s) 300
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 234
Eco47III AGCGCT 2 cut(s) 56, 516
Eco53kI GAGCTC 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 2 cut(s) 682, 728
EcoT14I CCWWGG 1 cut(s) 63
EcoT38I GRGCYC 1 cut(s) 234
EgeI GGCGCC 1 cut(s) 688
EheI GGCGCC 1 cut(s) 688
ErhI CCWWGG 1 cut(s) 63
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 4 cut(s) 44, 454, 638, 751
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FaqI GGGAC 1 cut(s) 338
FatI CATG 4 cut(s) 40, 450, 634, 747
FbaI TGATCA 1 cut(s) 186
FblI GTMKAC 1 cut(s) 125
FokI GGATG 1 cut(s) 541
FriOI GRGCYC 1 cut(s) 234
FspBI CTAG 2 cut(s) 239, 752
GlaI GCGC 4 cut(s) 56, 516, 630, 688
HaeII RGCGCY 3 cut(s) 58, 518, 690
HaeIII GGCC 2 cut(s) 243, 302
HapII CCGG 1 cut(s) 379
HhaI GCGC 4 cut(s) 57, 517, 631, 689
Hin1I GRCGYC 2 cut(s) 327, 687
Hin1II CATG 4 cut(s) 44, 454, 638, 751
Hin6I GCGC 4 cut(s) 55, 515, 629, 687
HinP1I GCGC 4 cut(s) 55, 515, 629, 687
HinfI GANTC 2 cut(s) 25, 646
HpaII CCGG 1 cut(s) 379
HphI GGTGA 2 cut(s) 154, 731
Hpy166II GTNNAC 2 cut(s) 126, 283
Hpy188I TCNGA 1 cut(s) 585
Hpy8I GTNNAC 2 cut(s) 126, 283
HpyAV CCTTC 3 cut(s) 175, 380, 494
HpyCH4III ACNGT 1 cut(s) 216
HpyCH4IV ACGT 4 cut(s) 294, 327, 602, 734
HpyCH4V TGCA 4 cut(s) 44, 283, 472, 667
HpyF10VI GCNNNNNNNGC 2 cut(s) 478, 596
HpyF3I CTNAG 5 cut(s) 58, 476, 482, 651, 756
HpySE526I ACGT 4 cut(s) 294, 327, 602, 734
Hsp92I GRCGYC 2 cut(s) 327, 687
Hsp92II CATG 4 cut(s) 44, 454, 638, 751
HspAI GCGC 4 cut(s) 55, 515, 629, 687
KasI GGCGCC 1 cut(s) 686
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 2 cut(s) 186, 505
LmnI GCTCC 2 cut(s) 229, 433
LweI GCATC 1 cut(s) 481
MaeI CTAG 2 cut(s) 239, 752
MaeII ACGT 4 cut(s) 294, 327, 602, 734
MalI GATC 2 cut(s) 188, 507
MboI GATC 2 cut(s) 186, 505
MboII GAAGA 2 cut(s) 347, 466
MhlI GDGCHC 2 cut(s) 234, 285
MluCI AATT 5 cut(s) 97, 158, 287, 339, 493
Mly113I GGCGCC 1 cut(s) 687
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 4 cut(s) 179, 373, 397, 660
MseI TTAA 2 cut(s) 218, 519
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 2 cut(s) 684, 730
MvaI CCWGG 2 cut(s) 684, 730
MwoI GCNNNNNNNGC 2 cut(s) 478, 596
NarI GGCGCC 1 cut(s) 687
NdeII GATC 2 cut(s) 186, 505
NheI GCTAGC 1 cut(s) 751
NlaIII CATG 4 cut(s) 44, 454, 638, 751
NlaIV GGNNCC 4 cut(s) 69, 244, 435, 688
NmeAIII GCCGAG 1 cut(s) 278
NspI RCATGY 2 cut(s) 44, 638
PaeI GCATGC 1 cut(s) 44
PciI ACATGT 1 cut(s) 634
PfeI GAWTC 2 cut(s) 25, 646
PflFI GACNNNGTC 1 cut(s) 121
PluTI GGCGCC 1 cut(s) 690
PscI ACATGT 1 cut(s) 634
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 682, 728
PspGI CCWGG 2 cut(s) 682, 728
PspN4I GGNNCC 4 cut(s) 69, 244, 435, 688
PspPI GGNCC 1 cut(s) 242
PsyI GACNNNGTC 1 cut(s) 121
RsaI GTAC 2 cut(s) 33, 726
RsaNI GTAC 2 cut(s) 32, 725
SacI GAGCTC 1 cut(s) 234
SaqAI TTAA 2 cut(s) 218, 519
Sau3AI GATC 2 cut(s) 186, 505
Sau96I GGNCC 1 cut(s) 242
ScrFI CCNGG 2 cut(s) 684, 730
SduI GDGCHC 2 cut(s) 234, 285
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 1 cut(s) 696
SfoI GGCGCC 1 cut(s) 688
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
SphI GCATGC 1 cut(s) 44
Sse9I AATT 5 cut(s) 97, 158, 287, 339, 493
SsiI CCGC 2 cut(s) 413, 590
SspDI GGCGCC 1 cut(s) 686
SspI AATATT 1 cut(s) 711
SspMI CTAG 2 cut(s) 239, 752
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 2 cut(s) 682, 728
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 1 cut(s) 216
TaiI ACGT 4 cut(s) 297, 330, 605, 737
TaqI TCGA 2 cut(s) 116, 423
TaqII GACCGA 1 cut(s) 431
TasI AATT 5 cut(s) 97, 158, 287, 339, 493
TfiI GAWTC 2 cut(s) 25, 646
Tru1I TTAA 2 cut(s) 218, 519
Tru9I TTAA 2 cut(s) 218, 519
TscAI CASTG 1 cut(s) 659
TspDTI ATGAA 2 cut(s) 467, 659
TspGWI ACGGA 1 cut(s) 268
TspRI CASTG 1 cut(s) 659
Tth111I GACNNNGTC 1 cut(s) 121
Vha464I CTTAAG 1 cut(s) 518
VneI GTGCAC 1 cut(s) 281
XapI RAATTY 2 cut(s) 158, 287
XceI RCATGY 2 cut(s) 44, 638
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XmiI GTMKAC 1 cut(s) 125
XspI CTAG 2 cut(s) 239, 752
ZraI GACGTC 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.