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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 2 cut(s) | 170, 194 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 124 |
| AgsI | TTSAA | 3 cut(s) | 107, 145, 247 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 107, 139 |
| Alw21I | GWGCWC | 2 cut(s) | 231, 243 |
| Alw44I | GTGCAC | 1 cut(s) | 239 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 22 |
| ApaLI | GTGCAC | 1 cut(s) | 239 |
| BaeGI | GKGCMC | 1 cut(s) | 243 |
| Bbv12I | GWGCWC | 2 cut(s) | 231, 243 |
| BccI | CCATC | 1 cut(s) | 276 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 177, 211 |
| BciVI | GTATCC | 1 cut(s) | 212 |
| BfuI | GTATCC | 1 cut(s) | 212 |
| BmiI | GGNNCC | 1 cut(s) | 12 |
| BmrI | ACTGGG | 1 cut(s) | 100 |
| BmuI | ACTGGG | 1 cut(s) | 100 |
| BsaJI | CCNNGG | 1 cut(s) | 6 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 124 |
| Bse1I | ACTGG | 2 cut(s) | 95, 240 |
| Bse3DI | GCAATG | 1 cut(s) | 195 |
| BseDI | CCNNGG | 1 cut(s) | 6 |
| BseGI | GGATG | 2 cut(s) | 287, 298 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 124 |
| BseMI | GCAATG | 1 cut(s) | 195 |
| BseMII | CTCAG | 2 cut(s) | 14, 29 |
| BseNI | ACTGG | 2 cut(s) | 95, 240 |
| BseSI | GKGCMC | 1 cut(s) | 243 |
| BsiHKAI | GWGCWC | 2 cut(s) | 231, 243 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 124 |
| Bsp1286I | GDGCHC | 2 cut(s) | 231, 243 |
| Bsp143I | GATC | 1 cut(s) | 297 |
| BspCNI | CTCAG | 2 cut(s) | 15, 28 |
| BspLI | GGNNCC | 1 cut(s) | 12 |
| BsrDI | GCAATG | 1 cut(s) | 195 |
| BsrI | ACTGG | 2 cut(s) | 95, 240 |
| BssECI | CCNNGG | 1 cut(s) | 6 |
| BssMI | GATC | 1 cut(s) | 297 |
| BssT1I | CCWWGG | 1 cut(s) | 6 |
| BstDEI | CTNAG | 2 cut(s) | 15, 23 |
| BstF5I | GGATG | 2 cut(s) | 287, 298 |
| BstKTI | GATC | 1 cut(s) | 300 |
| BstMBI | GATC | 1 cut(s) | 297 |
| BstSLI | GKGCMC | 1 cut(s) | 243 |
| BsuI | GTATCC | 1 cut(s) | 212 |
| BtsCI | GGATG | 2 cut(s) | 287, 298 |
| CaiI | CAGNNNCTG | 1 cut(s) | 22 |
| Csp6I | GTAC | 2 cut(s) | 169, 193 |
| CviJI | RGCY | 3 cut(s) | 86, 159, 205 |
| CviKI_1 | RGCY | 3 cut(s) | 86, 159, 205 |
| CviQI | GTAC | 2 cut(s) | 169, 193 |
| DdeI | CTNAG | 2 cut(s) | 15, 23 |
| DpnI | GATC | 1 cut(s) | 299 |
| DpnII | GATC | 1 cut(s) | 297 |
| Eco130I | CCWWGG | 1 cut(s) | 6 |
| Eco32I | GATATC | 1 cut(s) | 252 |
| EcoRV | GATATC | 1 cut(s) | 252 |
| EcoT14I | CCWWGG | 1 cut(s) | 6 |
| ErhI | CCWWGG | 1 cut(s) | 6 |
| FaiI | YATR | 2 cut(s) | 98, 152 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 125, 157 |
| FokI | GGATG | 2 cut(s) | 294, 305 |
| HinfI | GANTC | 3 cut(s) | 19, 38, 136 |
| Hpy166II | GTNNAC | 1 cut(s) | 241 |
| Hpy188I | TCNGA | 3 cut(s) | 18, 24, 264 |
| Hpy8I | GTNNAC | 1 cut(s) | 241 |
| HpyAV | CCTTC | 1 cut(s) | 113 |
| HpyCH4V | TGCA | 2 cut(s) | 188, 241 |
| HpyF3I | CTNAG | 2 cut(s) | 15, 23 |
| Kzo9I | GATC | 1 cut(s) | 297 |
| LmnI | GCTCC | 1 cut(s) | 226 |
| LpnPI | CCDG | 3 cut(s) | 76, 208, 221 |
| MaeIII | GTNAC | 1 cut(s) | 127 |
| MalI | GATC | 1 cut(s) | 299 |
| MboI | GATC | 1 cut(s) | 297 |
| MboII | GAAGA | 1 cut(s) | 259 |
| MhlI | GDGCHC | 2 cut(s) | 231, 243 |
| MlyI | GAGTC | 2 cut(s) | 13, 32 |
| MmeI | TCCRAC | 1 cut(s) | 55 |
| MnlI | CCTC | 1 cut(s) | 24 |
| MseI | TTAA | 1 cut(s) | 60 |
| NdeII | GATC | 1 cut(s) | 297 |
| NlaIV | GGNNCC | 1 cut(s) | 12 |
| NmuCI | GTSAC | 1 cut(s) | 127 |
| PfeI | GAWTC | 1 cut(s) | 136 |
| PleI | GAGTC | 2 cut(s) | 13, 32 |
| PpsI | GAGTC | 2 cut(s) | 13, 32 |
| PspN4I | GGNNCC | 1 cut(s) | 12 |
| PstNI | CAGNNNCTG | 1 cut(s) | 22 |
| RsaI | GTAC | 2 cut(s) | 170, 194 |
| RsaNI | GTAC | 2 cut(s) | 169, 193 |
| SaqAI | TTAA | 1 cut(s) | 60 |
| Sau3AI | GATC | 1 cut(s) | 297 |
| SchI | GAGTC | 2 cut(s) | 13, 32 |
| SduI | GDGCHC | 2 cut(s) | 231, 243 |
| StyI | CCWWGG | 1 cut(s) | 6 |
| TaqI | TCGA | 3 cut(s) | 36, 161, 197 |
| TatI | WGTACW | 1 cut(s) | 192 |
| TfiI | GAWTC | 1 cut(s) | 136 |
| Tru1I | TTAA | 1 cut(s) | 60 |
| Tru9I | TTAA | 1 cut(s) | 60 |
| TseFI | GTSAC | 1 cut(s) | 127 |
| Tsp45I | GTSAC | 1 cut(s) | 127 |
| VneI | GTGCAC | 1 cut(s) | 239 |