Rh5DG515600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001089
Physical Location & Seq
Forward (+)
80173330 .. 80174028
699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG515600.1

Sequence Viewer

Length: 699 bp
ATGGAAATTAACTTCAAAGCTCCGGAAGGCCCAGAAAGCTCACCCCTACTTCCAATTTCAAATAACCCTAATTCAAACCCTCATGTTCAGCAGTCAATGAAATCACACTACATTTCTAAAGTTACCGTAGTCCAACTCATAATTCTAATGGTTACATTTGGGACAGTCTTGTCCTTGCCTTCTTTTTTCACCTCCATGTCCTTGGTTCCCCAAACTCCAAGTTTCCAACTCGACTCTCTTTTTCTATCTAATTTTAACATCTCAAACACAATATTCGGGGCTGATTGGGATATAACCCTTGAAATAGAGAACCCCAACTTGGTCTCGACGGTTCATGTCAACAGCATAAAGGGCTCGATTTTGTACAACAACAACTTTCTTGCAATGTACTCGGTAGATCCATTTGAGTTGGGATACATGGAGCATAGACCGGTGCACATGAAGATATCAAAATATCGAACAATGACAATGAATAGATGGGTTTCAGACGAGATCAATAGGCAACGAGATGAGAATGCAATTGTGAGTTTCAACCTAACAATGTTTGTTTCAACTACATATACGACCGGCTGGTGGGGAGCTGAAAAGGTTGTATTGAACCCTCAGTGTTTGGATTTGAGGATTGCCTTCCTTTCTAAAACTGGGTTCGGAAGTTGGGTAAATGGAGGGCCAATGAAATGCTCACTTCCTATGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

232

Amino Acids

26.16

Weight (kDa)

6.19

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 169
AccIII TCCGGA 1 cut(s) 22
AclWI GGATC 1 cut(s) 392
AfaI GTAC 2 cut(s) 365, 389
AfiI CCNNNNNNNGG 2 cut(s) 319, 573
AgeI ACCGGT 1 cut(s) 430
AgsI TTSAA 7 cut(s) 16, 60, 75, 302, 532, 552, 598
AjuI GAANNNNNNNTTGG 4 cut(s) 126, 158, 302, 334
AluBI AGCT 3 cut(s) 20, 39, 581
AluI AGCT 3 cut(s) 20, 39, 581
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 1 cut(s) 328
Alw44I GTGCAC 1 cut(s) 434
AlwI GGATC 1 cut(s) 392
Aor13HI TCCGGA 1 cut(s) 22
AoxI GGCC 2 cut(s) 28, 668
ApaLI GTGCAC 1 cut(s) 434
AsiGI ACCGGT 1 cut(s) 430
AspS9I GGNCC 2 cut(s) 29, 668
AsuHPI GGTGA 2 cut(s) 33, 181
BaeGI GKGCMC 1 cut(s) 438
BanII GRGCYC 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 438
BccI CCATC 1 cut(s) 471
BcgI CGANNNNNNTGC 2 cut(s) 372, 406
BciVI GTATCC 1 cut(s) 407
BcoDI GTCTC 1 cut(s) 328
BfuI GTATCC 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 29, 668
BmiI GGNNCC 1 cut(s) 207
BmrI ACTGGG 1 cut(s) 651
BmuI ACTGGG 1 cut(s) 651
BsaI GGTCTC 1 cut(s) 328
BsaJI CCNNGG 1 cut(s) 201
BsaWI WCCGGW 2 cut(s) 22, 430
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 573
Bse118I RCCGGY 2 cut(s) 430, 566
Bse1I ACTGG 1 cut(s) 646
Bse3DI GCAATG 1 cut(s) 390
BseAI TCCGGA 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 201
BseLI CCNNNNNNNGG 2 cut(s) 319, 573
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 1 cut(s) 617
BseNI ACTGG 1 cut(s) 646
BseSI GKGCMC 1 cut(s) 438
Bsh1285I CGRYCG 1 cut(s) 567
BshFI GGCC 2 cut(s) 30, 670
BshTI ACCGGT 1 cut(s) 430
BsiEI CGRYCG 1 cut(s) 567
BsiHKAI GWGCWC 1 cut(s) 438
BsiSI CCGG 3 cut(s) 23, 431, 567
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 2 cut(s) 319, 573
BsmAI GTCTC 1 cut(s) 328
BsmFI GGGAC 1 cut(s) 175
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 2 cut(s) 30, 670
Bso31I GGTCTC 1 cut(s) 328
Bsp1286I GDGCHC 2 cut(s) 356, 438
Bsp13I TCCGGA 1 cut(s) 22
Bsp1407I TGTACA 1 cut(s) 363
Bsp143I GATC 2 cut(s) 397, 492
BspANI GGCC 2 cut(s) 30, 670
BspCNI CTCAG 1 cut(s) 616
BspEI TCCGGA 1 cut(s) 22
BspLI GGNNCC 1 cut(s) 207
BspPI GGATC 1 cut(s) 392
BspTNI GGTCTC 1 cut(s) 328
BsrDI GCAATG 1 cut(s) 390
BsrFI RCCGGY 2 cut(s) 430, 566
BsrGI TGTACA 1 cut(s) 363
BsrI ACTGG 1 cut(s) 646
BssAI RCCGGY 2 cut(s) 430, 566
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 2 cut(s) 397, 492
BssT1I CCWWGG 1 cut(s) 201
Bst4CI ACNGT 3 cut(s) 127, 166, 331
BstAUI TGTACA 1 cut(s) 363
BstDEI CTNAG 1 cut(s) 603
BstKTI GATC 2 cut(s) 400, 495
BstMAI GTCTC 1 cut(s) 328
BstMBI GATC 2 cut(s) 397, 492
BstMCI CGRYCG 1 cut(s) 567
BstMWI GCNNNNNNNGC 2 cut(s) 36, 351
BstSLI GKGCMC 1 cut(s) 438
BstX2I RGATCY 1 cut(s) 397
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 397
BsuI GTATCC 1 cut(s) 407
BsuRI GGCC 2 cut(s) 30, 670
BtsIMutI CAGTG 1 cut(s) 611
Cfr10I RCCGGY 2 cut(s) 430, 566
Cfr13I GGNCC 2 cut(s) 29, 668
Csp6I GTAC 2 cut(s) 364, 388
CspAI ACCGGT 1 cut(s) 430
CviAII CATG 5 cut(s) 83, 196, 335, 418, 439
CviJI RGCY 8 cut(s) 20, 30, 39, 281, 354, 570, 581, 670
CviKI_1 RGCY 8 cut(s) 20, 30, 39, 281, 354, 570, 581, 670
CviQI GTAC 2 cut(s) 364, 388
DdeI CTNAG 1 cut(s) 603
DpnI GATC 2 cut(s) 399, 494
DpnII GATC 2 cut(s) 397, 492
DrdI GACNNNNNNGTC 1 cut(s) 169
DseDI GACNNNNNNGTC 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 328
Eco32I GATATC 1 cut(s) 447
EcoRV GATATC 1 cut(s) 447
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 5 cut(s) 86, 199, 338, 421, 442
FaqI GGGAC 1 cut(s) 175
FatI CATG 5 cut(s) 82, 195, 334, 417, 438
FriOI GRGCYC 1 cut(s) 356
HaeIII GGCC 2 cut(s) 30, 670
HapII CCGG 3 cut(s) 23, 431, 567
Hin1II CATG 5 cut(s) 86, 199, 338, 421, 442
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 1 cut(s) 233
HpaII CCGG 3 cut(s) 23, 431, 567
HphI GGTGA 2 cut(s) 33, 181
Hpy166II GTNNAC 2 cut(s) 340, 436
Hpy188I TCNGA 2 cut(s) 487, 650
Hpy188III TCNNGA 3 cut(s) 23, 325, 696
Hpy8I GTNNAC 2 cut(s) 340, 436
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 3 cut(s) 20, 189, 637
HpyCH4III ACNGT 3 cut(s) 127, 166, 331
HpyCH4V TGCA 3 cut(s) 383, 436, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 351
HpyF3I CTNAG 1 cut(s) 603
Hsp92II CATG 5 cut(s) 86, 199, 338, 421, 442
Kpn2I TCCGGA 1 cut(s) 22
Kzo9I GATC 2 cut(s) 397, 492
LmnI GCTCC 3 cut(s) 25, 421, 578
LpnPI CCDG 6 cut(s) 36, 45, 444, 556, 580, 627
MaeIII GTNAC 2 cut(s) 121, 151
MalI GATC 2 cut(s) 399, 494
MboI GATC 2 cut(s) 397, 492
MboII GAAGA 1 cut(s) 454
MfeI CAATTG 1 cut(s) 519
MflI RGATCY 1 cut(s) 397
MhlI GDGCHC 2 cut(s) 356, 438
MluCI AATT 6 cut(s) 6, 54, 70, 141, 250, 519
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 2 cut(s) 157, 250
MnlI CCTC 5 cut(s) 90, 202, 612, 612, 659
MroI TCCGGA 1 cut(s) 22
MseI TTAA 2 cut(s) 9, 255
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 3 cut(s) 23, 431, 567
MunI CAATTG 1 cut(s) 519
Mva1269I GAATGC 1 cut(s) 520
MwoI GCNNNNNNNGC 2 cut(s) 36, 351
NdeII GATC 2 cut(s) 397, 492
NlaIII CATG 5 cut(s) 86, 199, 338, 421, 442
NlaIV GGNNCC 1 cut(s) 207
PctI GAATGC 1 cut(s) 520
PinAI ACCGGT 1 cut(s) 430
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 2 cut(s) 29, 668
PsuI RGATCY 1 cut(s) 397
RsaI GTAC 2 cut(s) 365, 389
RsaNI GTAC 2 cut(s) 364, 388
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 2 cut(s) 9, 255
Sau3AI GATC 2 cut(s) 397, 492
Sau96I GGNCC 2 cut(s) 29, 668
SchI GAGTC 1 cut(s) 227
SduI GDGCHC 2 cut(s) 356, 438
SetI ASST 6 cut(s) 22, 41, 194, 537, 583, 591
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 6 cut(s) 6, 54, 70, 141, 250, 519
SspI AATATT 1 cut(s) 273
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 3 cut(s) 127, 166, 331
TaqI TCGA 4 cut(s) 231, 326, 356, 457
TasI AATT 6 cut(s) 6, 54, 70, 141, 250, 519
TatI WGTACW 2 cut(s) 363, 387
Tru1I TTAA 2 cut(s) 9, 255
Tru9I TTAA 2 cut(s) 9, 255
TscAI CASTG 1 cut(s) 611
TspDTI ATGAA 5 cut(s) 113, 323, 455, 485, 689
TspRI CASTG 1 cut(s) 611
VneI GTGCAC 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.