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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 180 |
| AclWI | GGATC | 2 cut(s) | 53, 80 |
| AfaI | GTAC | 1 cut(s) | 131 |
| AflIII | ACRYGT | 1 cut(s) | 162 |
| AluBI | AGCT | 3 cut(s) | 41, 66, 78 |
| AluI | AGCT | 3 cut(s) | 41, 66, 78 |
| Alw21I | GWGCWC | 1 cut(s) | 37 |
| AlwI | GGATC | 2 cut(s) | 53, 80 |
| AsuHPI | GGTGA | 1 cut(s) | 25 |
| Bbv12I | GWGCWC | 1 cut(s) | 37 |
| BfmI | CTRYAG | 2 cut(s) | 174, 199 |
| BfuAI | ACCTGC | 1 cut(s) | 180 |
| BoxI | GACNNNNGTC | 1 cut(s) | 201 |
| Bpu10I | CCTNAGC | 1 cut(s) | 67 |
| BpuEI | CTTGAG | 2 cut(s) | 21, 104 |
| BseMII | CTCAG | 1 cut(s) | 81 |
| BsgI | GTGCAG | 1 cut(s) | 129 |
| BsiHKAI | GWGCWC | 1 cut(s) | 37 |
| Bsp1286I | GDGCHC | 1 cut(s) | 37 |
| Bsp143I | GATC | 2 cut(s) | 45, 72 |
| BspCNI | CTCAG | 1 cut(s) | 80 |
| BspMAI | CTGCAG | 2 cut(s) | 178, 203 |
| BspMI | ACCTGC | 1 cut(s) | 180 |
| BspPI | GGATC | 2 cut(s) | 53, 80 |
| BssMI | GATC | 2 cut(s) | 45, 72 |
| Bst4CI | ACNGT | 2 cut(s) | 124, 225 |
| BstC8I | GCNNGC | 1 cut(s) | 112 |
| BstDEI | CTNAG | 1 cut(s) | 67 |
| BstKTI | GATC | 2 cut(s) | 48, 75 |
| BstMBI | GATC | 2 cut(s) | 45, 72 |
| BstNSI | RCATGY | 1 cut(s) | 166 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 201 |
| BstSFI | CTRYAG | 2 cut(s) | 174, 199 |
| BstX2I | RGATCY | 1 cut(s) | 45 |
| BstYI | RGATCY | 1 cut(s) | 45 |
| BtsIMutI | CAGTG | 1 cut(s) | 129 |
| BveI | ACCTGC | 1 cut(s) | 180 |
| Cac8I | GCNNGC | 1 cut(s) | 112 |
| Csp6I | GTAC | 1 cut(s) | 130 |
| CviAII | CATG | 3 cut(s) | 57, 133, 163 |
| CviJI | RGCY | 3 cut(s) | 41, 66, 78 |
| CviKI_1 | RGCY | 3 cut(s) | 41, 66, 78 |
| CviQI | GTAC | 1 cut(s) | 130 |
| DdeI | CTNAG | 1 cut(s) | 67 |
| DpnI | GATC | 2 cut(s) | 47, 74 |
| DpnII | GATC | 2 cut(s) | 45, 72 |
| FaeI | CATG | 3 cut(s) | 60, 136, 166 |
| FaiI | YATR | 4 cut(s) | 58, 134, 164, 186 |
| FatI | CATG | 3 cut(s) | 56, 132, 162 |
| Hin1II | CATG | 3 cut(s) | 60, 136, 166 |
| HincII | GTYRAC | 1 cut(s) | 206 |
| HindII | GTYRAC | 1 cut(s) | 206 |
| HphI | GGTGA | 1 cut(s) | 25 |
| Hpy166II | GTNNAC | 1 cut(s) | 206 |
| Hpy188I | TCNGA | 1 cut(s) | 45 |
| Hpy188III | TCNNGA | 1 cut(s) | 83 |
| Hpy8I | GTNNAC | 1 cut(s) | 206 |
| HpyAV | CCTTC | 1 cut(s) | 3 |
| HpyCH4III | ACNGT | 2 cut(s) | 124, 225 |
| HpyCH4V | TGCA | 4 cut(s) | 56, 110, 176, 201 |
| HpyF3I | CTNAG | 1 cut(s) | 67 |
| Hsp92II | CATG | 3 cut(s) | 60, 136, 166 |
| Kzo9I | GATC | 2 cut(s) | 45, 72 |
| LpnPI | CCDG | 5 cut(s) | 8, 54, 96, 162, 175 |
| MaeIII | GTNAC | 1 cut(s) | 219 |
| MalI | GATC | 2 cut(s) | 47, 74 |
| MboI | GATC | 2 cut(s) | 45, 72 |
| MboII | GAAGA | 2 cut(s) | 18, 40 |
| MflI | RGATCY | 1 cut(s) | 45 |
| MhlI | GDGCHC | 1 cut(s) | 37 |
| MmeI | TCCRAC | 1 cut(s) | 174 |
| MslI | CAYNNNNRTG | 1 cut(s) | 167 |
| NdeII | GATC | 2 cut(s) | 45, 72 |
| NlaIII | CATG | 3 cut(s) | 60, 136, 166 |
| NspI | RCATGY | 1 cut(s) | 166 |
| PciI | ACATGT | 1 cut(s) | 162 |
| PscI | ACATGT | 1 cut(s) | 162 |
| PshAI | GACNNNNGTC | 1 cut(s) | 201 |
| PstI | CTGCAG | 2 cut(s) | 178, 203 |
| PsuI | RGATCY | 1 cut(s) | 45 |
| RsaI | GTAC | 1 cut(s) | 131 |
| RsaNI | GTAC | 1 cut(s) | 130 |
| RseI | CAYNNNNRTG | 1 cut(s) | 167 |
| Sau3AI | GATC | 2 cut(s) | 45, 72 |
| SduI | GDGCHC | 1 cut(s) | 37 |
| SetI | ASST | 6 cut(s) | 22, 43, 68, 80, 194, 215 |
| SfcI | CTRYAG | 2 cut(s) | 174, 199 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 167 |
| SmlI | CTYRAG | 2 cut(s) | 36, 83 |
| SmoI | CTYRAG | 2 cut(s) | 36, 83 |
| TaaI | ACNGT | 2 cut(s) | 124, 225 |
| TatI | WGTACW | 1 cut(s) | 129 |
| TscAI | CASTG | 1 cut(s) | 129 |
| TspGWI | ACGGA | 1 cut(s) | 157 |
| TspRI | CASTG | 1 cut(s) | 129 |
| XceI | RCATGY | 1 cut(s) | 166 |