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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 2 cut(s) | 170, 194 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 124, 378, 446 |
| AgsI | TTSAA | 3 cut(s) | 24, 337, 403 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 107, 139 |
| AluBI | AGCT | 1 cut(s) | 359 |
| AluI | AGCT | 1 cut(s) | 359 |
| Alw21I | GWGCWC | 1 cut(s) | 243 |
| Alw26I | GTCTC | 1 cut(s) | 133 |
| Alw44I | GTGCAC | 1 cut(s) | 239 |
| AoxI | GGCC | 1 cut(s) | 473 |
| ApaLI | GTGCAC | 1 cut(s) | 239 |
| AspS9I | GGNCC | 1 cut(s) | 473 |
| AsuHPI | GGTGA | 1 cut(s) | 104 |
| BaeGI | GKGCMC | 1 cut(s) | 243 |
| BanII | GRGCYC | 1 cut(s) | 161 |
| Bbv12I | GWGCWC | 1 cut(s) | 243 |
| BccI | CCATC | 1 cut(s) | 276 |
| BciVI | GTATCC | 1 cut(s) | 212 |
| BcoDI | GTCTC | 1 cut(s) | 133 |
| BfuI | GTATCC | 1 cut(s) | 212 |
| BmgT120I | GGNCC | 1 cut(s) | 473 |
| BmiI | GGNNCC | 1 cut(s) | 12 |
| BsaI | GGTCTC | 1 cut(s) | 133 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 124, 378, 446 |
| Bse118I | RCCGGY | 1 cut(s) | 371 |
| Bse1I | ACTGG | 1 cut(s) | 236 |
| Bse3DI | GCAATG | 1 cut(s) | 195 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 124, 378, 446 |
| BseMI | GCAATG | 1 cut(s) | 195 |
| BseMII | CTCAG | 1 cut(s) | 422 |
| BseNI | ACTGG | 1 cut(s) | 236 |
| BseSI | GKGCMC | 1 cut(s) | 243 |
| BseYI | CCCAGC | 1 cut(s) | 446 |
| Bsh1285I | CGRYCG | 1 cut(s) | 372 |
| BshFI | GGCC | 1 cut(s) | 475 |
| BsiEI | CGRYCG | 1 cut(s) | 372 |
| BsiHKAI | GWGCWC | 1 cut(s) | 243 |
| BsiSI | CCGG | 1 cut(s) | 372 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 124, 378, 446 |
| BsmAI | GTCTC | 1 cut(s) | 133 |
| BsnI | GGCC | 1 cut(s) | 475 |
| Bso31I | GGTCTC | 1 cut(s) | 133 |
| Bsp1286I | GDGCHC | 2 cut(s) | 161, 243 |
| Bsp143I | GATC | 1 cut(s) | 297 |
| BspANI | GGCC | 1 cut(s) | 475 |
| BspCNI | CTCAG | 1 cut(s) | 421 |
| BspLI | GGNNCC | 1 cut(s) | 12 |
| BspTNI | GGTCTC | 1 cut(s) | 133 |
| BsrDI | GCAATG | 1 cut(s) | 195 |
| BsrFI | RCCGGY | 1 cut(s) | 371 |
| BsrI | ACTGG | 1 cut(s) | 236 |
| BssAI | RCCGGY | 1 cut(s) | 371 |
| BssMI | GATC | 1 cut(s) | 297 |
| Bst4CI | ACNGT | 2 cut(s) | 92, 136 |
| BstDEI | CTNAG | 1 cut(s) | 408 |
| BstKTI | GATC | 1 cut(s) | 300 |
| BstMAI | GTCTC | 1 cut(s) | 133 |
| BstMBI | GATC | 1 cut(s) | 297 |
| BstMCI | CGRYCG | 1 cut(s) | 372 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 156 |
| BstSLI | GKGCMC | 1 cut(s) | 243 |
| BsuI | GTATCC | 1 cut(s) | 212 |
| BsuRI | GGCC | 1 cut(s) | 475 |
| BtsIMutI | CAGTG | 2 cut(s) | 243, 416 |
| Cfr10I | RCCGGY | 1 cut(s) | 371 |
| Cfr13I | GGNCC | 1 cut(s) | 473 |
| Csp6I | GTAC | 2 cut(s) | 169, 193 |
| CviAII | CATG | 3 cut(s) | 140, 223, 244 |
| CviJI | RGCY | 6 cut(s) | 86, 159, 205, 359, 375, 475 |
| CviKI_1 | RGCY | 6 cut(s) | 86, 159, 205, 359, 375, 475 |
| CviQI | GTAC | 2 cut(s) | 169, 193 |
| DdeI | CTNAG | 1 cut(s) | 408 |
| DpnI | GATC | 1 cut(s) | 299 |
| DpnII | GATC | 1 cut(s) | 297 |
| Eco24I | GRGCYC | 1 cut(s) | 161 |
| Eco31I | GGTCTC | 1 cut(s) | 133 |
| Eco32I | GATATC | 1 cut(s) | 252 |
| EcoRV | GATATC | 1 cut(s) | 252 |
| EcoT38I | GRGCYC | 1 cut(s) | 161 |
| FaeI | CATG | 3 cut(s) | 143, 226, 247 |
| FaiI | YATR | 9 cut(s) | 98, 141, 152, 224, 231, 245, 364, 366, 497 |
| FatI | CATG | 3 cut(s) | 139, 222, 243 |
| FriOI | GRGCYC | 1 cut(s) | 161 |
| GsaI | CCCAGC | 1 cut(s) | 450 |
| HaeIII | GGCC | 1 cut(s) | 475 |
| HapII | CCGG | 1 cut(s) | 372 |
| Hin1II | CATG | 3 cut(s) | 143, 226, 247 |
| HincII | GTYRAC | 1 cut(s) | 145 |
| HindII | GTYRAC | 1 cut(s) | 145 |
| HinfI | GANTC | 1 cut(s) | 38 |
| HpaII | CCGG | 1 cut(s) | 372 |
| HphI | GGTGA | 1 cut(s) | 104 |
| Hpy166II | GTNNAC | 2 cut(s) | 145, 241 |
| Hpy188I | TCNGA | 1 cut(s) | 292 |
| Hpy188III | TCNNGA | 2 cut(s) | 130, 501 |
| Hpy8I | GTNNAC | 2 cut(s) | 145, 241 |
| HpyAV | CCTTC | 2 cut(s) | 442, 446 |
| HpyCH4III | ACNGT | 2 cut(s) | 92, 136 |
| HpyCH4IV | ACGT | 1 cut(s) | 457 |
| HpyCH4V | TGCA | 2 cut(s) | 188, 241 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 156 |
| HpyF3I | CTNAG | 1 cut(s) | 408 |
| HpySE526I | ACGT | 1 cut(s) | 457 |
| Hsp92II | CATG | 3 cut(s) | 143, 226, 247 |
| Kzo9I | GATC | 1 cut(s) | 297 |
| LpnPI | CCDG | 5 cut(s) | 249, 353, 361, 385, 432 |
| MaeII | ACGT | 1 cut(s) | 457 |
| MalI | GATC | 1 cut(s) | 299 |
| MboI | GATC | 1 cut(s) | 297 |
| MboII | GAAGA | 2 cut(s) | 259, 403 |
| MhlI | GDGCHC | 2 cut(s) | 161, 243 |
| MlyI | GAGTC | 1 cut(s) | 32 |
| MmeI | TCCRAC | 1 cut(s) | 55 |
| MnlI | CCTC | 3 cut(s) | 417, 417, 464 |
| MseI | TTAA | 2 cut(s) | 60, 105 |
| MspI | CCGG | 1 cut(s) | 372 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 156 |
| NdeII | GATC | 1 cut(s) | 297 |
| NlaIII | CATG | 3 cut(s) | 143, 226, 247 |
| NlaIV | GGNNCC | 1 cut(s) | 12 |
| PleI | GAGTC | 1 cut(s) | 32 |
| PpsI | GAGTC | 1 cut(s) | 32 |
| PspFI | CCCAGC | 1 cut(s) | 446 |
| PspN4I | GGNNCC | 1 cut(s) | 12 |
| PspPI | GGNCC | 1 cut(s) | 473 |
| RsaI | GTAC | 2 cut(s) | 170, 194 |
| RsaNI | GTAC | 2 cut(s) | 169, 193 |
| SaqAI | TTAA | 2 cut(s) | 60, 105 |
| Sau3AI | GATC | 1 cut(s) | 297 |
| Sau96I | GGNCC | 1 cut(s) | 473 |
| SchI | GAGTC | 1 cut(s) | 32 |
| SduI | GDGCHC | 2 cut(s) | 161, 243 |
| SetI | ASST | 3 cut(s) | 342, 361, 460 |
| TaaI | ACNGT | 2 cut(s) | 92, 136 |
| TaiI | ACGT | 1 cut(s) | 460 |
| TaqI | TCGA | 4 cut(s) | 36, 131, 161, 262 |
| TatI | WGTACW | 1 cut(s) | 192 |
| Tru1I | TTAA | 2 cut(s) | 60, 105 |
| Tru9I | TTAA | 2 cut(s) | 60, 105 |
| TscAI | CASTG | 2 cut(s) | 243, 416 |
| TspDTI | ATGAA | 5 cut(s) | 128, 260, 290, 330, 494 |
| TspRI | CASTG | 2 cut(s) | 243, 416 |
| VneI | GTGCAC | 1 cut(s) | 239 |