MD14G1037800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001089
Physical Location & Seq
Reverse (-)
3455705 .. 3456445
741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1037800.v1.1.491

Sequence Viewer

Length: 741 bp
ATGGAAATTAATTCAAAAGCTCATCCCGCAGCCACTACGCCACTACTCCCCGTTTCAAATAACCCTTCTTCAAACCCTAACACTTCTCAGGCTATTATTAGATGGATTGCCGGTTTGGTCCTTGTCCAACTCATCAATGTTCTATTAATTATATTTATGTTAGGAACCTTCACAATTTTTTGCAATTCCATGCCCTTGTCCCTAGCTCCACAACCCCCAAATGTCCAACTCAATTCCCTCTTCATGTCCAAATTTAACACCTCCAGCACAACCCTAGGGGCTAATTGGGAAATGTCCCTTACGGTAGAGAACCCTAATCTTGTCACATGGGTTCGTTTCAACCACGTAAAGGGCTCGATTTCGTACAAAGATTATTTCGTCGCGATAAGCTCTGTGGAGCCGTTTGAATTGGGATTGAAGGAAAGTAAAATGATTCGCTTGAAGATATCGTCGAAGATGACAGGGTCGGAGGAGGACTATCATCGCCTTGGTCTGCCGGTGGTGATCATGAAGTGGGGTTTGGATGAGATGCTGAGTAGGGAGCGTGAGGATGGAGCTGTGCGTTTTAACTTGCAAATGTTTGTTTGGGCAACTTATAAGACTGGTTGGTGGGGGGAACAACATGTTGTAATGAGCCCTCAGTGTTTGGATATGAAGGTTGAATTATCGCCTAGAAATGGTTTCGGAAGTTGGATTAGTGGAGGGCCTATGAGGTGCTCGGTTCCAGTACTAATTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

27.54

Weight (kDa)

8.44

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 597
AccII CGCG 1 cut(s) 383
AciI CCGC 1 cut(s) 27
AcsI RAATTY 1 cut(s) 251
AfaI GTAC 2 cut(s) 365, 729
AfiI CCNNNNNNNGG 2 cut(s) 349, 677
AflIII ACRYGT 1 cut(s) 622
AgsI TTSAA 8 cut(s) 15, 57, 72, 340, 407, 418, 442, 662
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
AluBI AGCT 4 cut(s) 20, 206, 390, 557
AluI AGCT 4 cut(s) 20, 206, 390, 557
Alw21I GWGCWC 1 cut(s) 719
AoxI GGCC 1 cut(s) 704
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 251
AseI ATTAAT 2 cut(s) 9, 146
AspA2I CCTAGG 1 cut(s) 274
AspS9I GGNCC 2 cut(s) 118, 704
AsuHPI GGTGA 1 cut(s) 514
AvaII GGWCC 1 cut(s) 118
AvrII CCTAGG 1 cut(s) 274
BanII GRGCYC 2 cut(s) 356, 638
Bbv12I GWGCWC 1 cut(s) 719
BbvI GCAGC 1 cut(s) 41
BccI CCATC 2 cut(s) 96, 545
BceAI ACGGC 1 cut(s) 385
BclI TGATCA 1 cut(s) 504
BfaI CTAG 3 cut(s) 203, 275, 672
BisI GCNGC 1 cut(s) 30
BlnI CCTAGG 1 cut(s) 274
BlsI GCNGC 1 cut(s) 31
BmcAI AGTACT 1 cut(s) 729
Bme18I GGWCC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 118, 704
BmiI GGNNCC 3 cut(s) 166, 399, 723
BmsI GCATC 1 cut(s) 519
BpmI CTGGAG 1 cut(s) 247
BsaAI YACGTR 1 cut(s) 346
BsaJI CCNNGG 2 cut(s) 274, 487
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 677
Bse118I RCCGGY 2 cut(s) 110, 496
Bse1I ACTGG 2 cut(s) 607, 725
BseDI CCNNGG 2 cut(s) 274, 487
BseGI GGATG 3 cut(s) 22, 529, 556
BseLI CCNNNNNNNGG 2 cut(s) 349, 677
BseMII CTCAG 3 cut(s) 101, 524, 653
BseNI ACTGG 2 cut(s) 607, 725
BseRI GAGGAG 1 cut(s) 485
BseXI GCAGC 1 cut(s) 41
Bsh1236I CGCG 1 cut(s) 383
BshFI GGCC 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 719
BsiSI CCGG 2 cut(s) 111, 497
BslFI GGGAC 2 cut(s) 184, 280
BslI CCNNNNNNNGG 2 cut(s) 349, 677
BsmFI GGGAC 2 cut(s) 184, 280
BsnI GGCC 1 cut(s) 706
Bsp1286I GDGCHC 3 cut(s) 356, 638, 719
Bsp143I GATC 1 cut(s) 504
Bsp68I TCGCGA 1 cut(s) 383
BspACI CCGC 1 cut(s) 27
BspANI GGCC 1 cut(s) 706
BspCNI CTCAG 3 cut(s) 100, 525, 652
BspFNI CGCG 1 cut(s) 383
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 3 cut(s) 166, 399, 723
BsrFI RCCGGY 2 cut(s) 110, 496
BsrI ACTGG 2 cut(s) 607, 725
BssAI RCCGGY 2 cut(s) 110, 496
BssECI CCNNGG 2 cut(s) 274, 487
BssMI GATC 1 cut(s) 504
BssT1I CCWWGG 2 cut(s) 274, 487
Bst4CI ACNGT 1 cut(s) 304
Bst6I CTCTTC 1 cut(s) 245
BstBAI YACGTR 1 cut(s) 346
BstDEI CTNAG 3 cut(s) 87, 533, 639
BstF5I GGATG 3 cut(s) 22, 529, 556
BstFNI CGCG 1 cut(s) 383
BstKTI GATC 1 cut(s) 507
BstMBI GATC 1 cut(s) 504
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNSI RCATGY 1 cut(s) 626
BstUI CGCG 1 cut(s) 383
BstV1I GCAGC 1 cut(s) 41
BsuRI GGCC 1 cut(s) 706
BtgZI GCGATG 1 cut(s) 467
BtsCI GGATG 3 cut(s) 22, 529, 556
BtsIMutI CAGTG 1 cut(s) 647
BtuMI TCGCGA 1 cut(s) 383
CciI TCATGA 1 cut(s) 507
Cfr10I RCCGGY 2 cut(s) 110, 496
Cfr13I GGNCC 2 cut(s) 118, 704
Csp6I GTAC 2 cut(s) 364, 728
CviAII CATG 5 cut(s) 190, 244, 327, 508, 623
CviQI GTAC 2 cut(s) 364, 728
DdeI CTNAG 3 cut(s) 87, 533, 639
DpnI GATC 1 cut(s) 506
DpnII GATC 1 cut(s) 504
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Eco130I CCWWGG 2 cut(s) 274, 487
Eco24I GRGCYC 2 cut(s) 356, 638
Eco32I GATATC 1 cut(s) 447
Eco47I GGWCC 1 cut(s) 118
EcoO109I RGGNCCY 1 cut(s) 704
EcoRV GATATC 1 cut(s) 447
EcoT14I CCWWGG 2 cut(s) 274, 487
EcoT38I GRGCYC 2 cut(s) 356, 638
ErhI CCWWGG 2 cut(s) 274, 487
FaeI CATG 5 cut(s) 193, 247, 330, 511, 626
FaqI GGGAC 2 cut(s) 184, 280
FatI CATG 5 cut(s) 189, 243, 326, 507, 622
FauI CCCGC 1 cut(s) 34
FbaI TGATCA 1 cut(s) 504
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 3 cut(s) 9, 536, 563
FriOI GRGCYC 2 cut(s) 356, 638
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 3 cut(s) 203, 275, 672
GluI GCNGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 247
HaeIII GGCC 1 cut(s) 706
HapII CCGG 2 cut(s) 111, 497
Hin1II CATG 5 cut(s) 193, 247, 330, 511, 626
HinfI GANTC 1 cut(s) 433
HpaII CCGG 2 cut(s) 111, 497
HphI GGTGA 1 cut(s) 514
Hpy188I TCNGA 2 cut(s) 469, 686
Hpy188III TCNNGA 2 cut(s) 382, 508
Hpy99I CGWCG 2 cut(s) 383, 454
HpyAV CCTTC 4 cut(s) 75, 178, 412, 649
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 2 cut(s) 183, 574
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 3 cut(s) 87, 533, 639
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 5 cut(s) 193, 247, 330, 511, 626
Ksp22I TGATCA 1 cut(s) 504
Kzo9I GATC 1 cut(s) 504
LmnI GCTCC 4 cut(s) 211, 397, 541, 554
LpnPI CCDG 6 cut(s) 74, 124, 277, 447, 510, 588
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 1 cut(s) 519
MaeI CTAG 3 cut(s) 203, 275, 672
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 322
MalI GATC 1 cut(s) 506
MboI GATC 1 cut(s) 504
MboII GAAGA 4 cut(s) 60, 232, 454, 466
MhlI GDGCHC 3 cut(s) 356, 638, 719
MmeI TCCRAC 4 cut(s) 151, 250, 447, 671
MnlI CCTC 8 cut(s) 248, 271, 463, 466, 541, 648, 695, 705
MseI TTAA 4 cut(s) 9, 146, 255, 567
MspI CCGG 2 cut(s) 111, 497
MvnI CGCG 1 cut(s) 383
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeII GATC 1 cut(s) 504
NlaIII CATG 5 cut(s) 193, 247, 330, 511, 626
NlaIV GGNNCC 3 cut(s) 166, 399, 723
NmuCI GTSAC 1 cut(s) 322
NruI TCGCGA 1 cut(s) 383
NspI RCATGY 1 cut(s) 626
PagI TCATGA 1 cut(s) 507
PciI ACATGT 1 cut(s) 622
PfeI GAWTC 1 cut(s) 433
PflFI GACNNNGTC 1 cut(s) 463
PkrI GCNGC 1 cut(s) 31
Ppu21I YACGTR 1 cut(s) 346
PscI ACATGT 1 cut(s) 622
PshBI ATTAAT 2 cut(s) 9, 146
PsiI TTATAA 1 cut(s) 597
PspN4I GGNNCC 3 cut(s) 166, 399, 723
PspPI GGNCC 2 cut(s) 118, 704
PsyI GACNNNGTC 1 cut(s) 463
RruI TCGCGA 1 cut(s) 383
RsaI GTAC 2 cut(s) 365, 729
RsaNI GTAC 2 cut(s) 364, 728
SaqAI TTAA 4 cut(s) 9, 146, 255, 567
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 1 cut(s) 504
Sau96I GGNCC 2 cut(s) 118, 704
ScaI AGTACT 1 cut(s) 729
SduI GDGCHC 3 cut(s) 356, 638, 719
SetI ASST 9 cut(s) 22, 170, 208, 263, 348, 392, 559, 660, 716
SfaNI GCATC 1 cut(s) 519
SinI GGWCC 1 cut(s) 118
SsiI CCGC 1 cut(s) 27
SspMI CTAG 3 cut(s) 203, 275, 672
StyI CCWWGG 2 cut(s) 274, 487
TaaI ACNGT 1 cut(s) 304
TaiI ACGT 1 cut(s) 348
TaqI TCGA 2 cut(s) 356, 452
TatI WGTACW 1 cut(s) 727
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 4 cut(s) 9, 146, 255, 567
Tru9I TTAA 4 cut(s) 9, 146, 255, 567
TscAI CASTG 1 cut(s) 647
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 3 cut(s) 232, 524, 668
TspRI CASTG 1 cut(s) 647
Tth111I GACNNNGTC 1 cut(s) 463
VpaK11BI GGWCC 1 cut(s) 118
VspI ATTAAT 2 cut(s) 9, 146
XapI RAATTY 1 cut(s) 251
XceI RCATGY 1 cut(s) 626
XmaJI CCTAGG 1 cut(s) 274
XspI CTAG 3 cut(s) 203, 275, 672
ZrmI AGTACT 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.