Rmu_sc0000539.1_g000081

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000539.1
OG0001089
Physical Location & Seq
Reverse (-)
348376 .. 349098
723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000539.1_g000081.1.cds

Sequence Viewer

Length: 723 bp
atggatgcaaaaggtcagctatcaattaccatgccgcttctcccagtttcgaataactcttcaaagccttacatttcgcgttcttttggatcacattacattcccgatttcacccttagccagctcatgattttgttaatcacattaggaaccattttgtccttgccatgcatattcatgtctatgtcccaatttccgcaaaccccaaacatccagctccattcgctcttcatttccaaactcaacatctccaacacaacgttaggggctaattgggacttgacccttaaaatagagaatcccaacttagtctcgtggattcgcttcaacgacataaaaggctcaattttgtacaagaataaccatctcgtaatgtactccgtggagccgtttgtattgggattgaaggaaaacagaattatgcaaatgaaaatgtcaaaccataagctggaaatgaacaaatgggttttggaagaaattggtaagcaataccaagatggcggagctgtccagtttagcctgcaaatgtctttttggggtatgtatagaaatgggtggaggggaaaacagaattttataatgaatcctcgatgttcggatttgaaggttggcttcttgcctcaaacagggttcggaacctggttaaggaaaggagaacatatgatgtgctctgttaatccaaatgaagacaaagtggatggctgtgagcctgtgataaggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

240

Amino Acids

27.46

Weight (kDa)

9.16

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 578
AccB7I CCANNNNNTGG 1 cut(s) 448
AccII CGCG 1 cut(s) 79
AciI CCGC 3 cut(s) 35, 197, 501
AclI AACGTT 1 cut(s) 260
AclWI GGATC 1 cut(s) 97
AcsI RAATTY 1 cut(s) 571
AfaI GTAC 2 cut(s) 353, 377
AfiI CCNNNNNNNGG 3 cut(s) 448, 626, 645
AgsI TTSAA 4 cut(s) 63, 328, 406, 604
AjnI CCWGG 1 cut(s) 638
AluBI AGCT 5 cut(s) 19, 124, 217, 448, 506
AluI AGCT 5 cut(s) 19, 124, 217, 448, 506
Alw21I GWGCWC 1 cut(s) 671
Alw26I GTCTC 1 cut(s) 316
AlwI GGATC 1 cut(s) 97
ApoI RAATTY 1 cut(s) 571
AsuHPI GGTGA 1 cut(s) 103
AsuII TTCGAA 1 cut(s) 50
BauI CACGAG 1 cut(s) 313
BbsI GAAGAC 1 cut(s) 693
Bbv12I GWGCWC 1 cut(s) 671
BccI CCATC 3 cut(s) 372, 491, 692
BceAI ACGGC 1 cut(s) 373
BciT130I CCWGG 1 cut(s) 640
BcoDI GTCTC 1 cut(s) 316
BisI GCNGC 1 cut(s) 35
BlsI GCNGC 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 640
BmiI GGNNCC 3 cut(s) 151, 387, 637
BmrFI CCNGG 1 cut(s) 640
BmrI ACTGGG 1 cut(s) 38
BmuI ACTGGG 1 cut(s) 38
BpiI GAAGAC 1 cut(s) 693
Bpu10I CCTNAGC 1 cut(s) 116
Bpu14I TTCGAA 1 cut(s) 50
BsaJI CCNNGG 1 cut(s) 381
Bsc4I CCNNNNNNNGG 3 cut(s) 448, 626, 645
Bse1I ACTGG 2 cut(s) 44, 511
BseBI CCWGG 1 cut(s) 640
BseDI CCNNGG 1 cut(s) 381
BseGI GGATG 3 cut(s) 10, 210, 703
BseLI CCNNNNNNNGG 3 cut(s) 448, 626, 645
BseNI ACTGG 2 cut(s) 44, 511
Bsh1236I CGCG 1 cut(s) 79
BsiHKAI GWGCWC 1 cut(s) 671
BslFI GGGAC 2 cut(s) 172, 290
BslI CCNNNNNNNGG 3 cut(s) 448, 626, 645
BsmAI GTCTC 1 cut(s) 316
BsmFI GGGAC 2 cut(s) 172, 290
Bsp119I TTCGAA 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 671
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 1 cut(s) 89
BspACI CCGC 3 cut(s) 35, 197, 501
BspFNI CGCG 1 cut(s) 79
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 151, 387, 637
BspPI GGATC 1 cut(s) 97
BspQI GCTCTTC 1 cut(s) 233
BspT104I TTCGAA 1 cut(s) 50
BsrGI TGTACA 1 cut(s) 351
BsrI ACTGG 2 cut(s) 44, 511
BssECI CCNNGG 1 cut(s) 381
BssMI GATC 1 cut(s) 89
BssSI CACGAG 1 cut(s) 313
Bst2BI CACGAG 1 cut(s) 313
Bst2UI CCWGG 1 cut(s) 640
Bst6I CTCTTC 2 cut(s) 64, 233
BstAUI TGTACA 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 50
BstC8I GCNNGC 2 cut(s) 122, 521
BstDEI CTNAG 2 cut(s) 116, 307
BstDSI CCRYGG 1 cut(s) 381
BstENI CCTNNNNNAGG 2 cut(s) 624, 643
BstF5I GGATG 3 cut(s) 10, 210, 703
BstFNI CGCG 1 cut(s) 79
BstKTI GATC 1 cut(s) 92
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 1 cut(s) 89
BstMWI GCNNNNNNNGC 1 cut(s) 223
BstNI CCWGG 1 cut(s) 640
BstSCI CCNGG 1 cut(s) 638
BstUI CGCG 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 693
BtgI CCRYGG 1 cut(s) 381
BtsCI GGATG 3 cut(s) 10, 210, 703
Cac8I GCNNGC 2 cut(s) 122, 521
CciI TCATGA 1 cut(s) 126
CsiI ACCWGGT 1 cut(s) 638
Csp6I GTAC 2 cut(s) 352, 376
CviAII CATG 4 cut(s) 31, 127, 168, 178
CviQI GTAC 2 cut(s) 352, 376
DdeI CTNAG 2 cut(s) 116, 307
DpnI GATC 1 cut(s) 91
DpnII GATC 1 cut(s) 89
Eam1104I CTCTTC 2 cut(s) 64, 233
EarI CTCTTC 2 cut(s) 64, 233
EciI GGCGGA 1 cut(s) 516
EcoNI CCTNNNNNAGG 2 cut(s) 624, 643
EcoRII CCWGG 1 cut(s) 638
EcoT22I ATGCAT 1 cut(s) 173
FaeI CATG 4 cut(s) 34, 130, 171, 181
FalI AAGNNNNNCTT 2 cut(s) 596, 628
FaqI GGGAC 2 cut(s) 172, 290
FatI CATG 4 cut(s) 30, 126, 167, 177
FauNDI CATATG 1 cut(s) 660
Fnu4HI GCNGC 1 cut(s) 35
FokI GGATG 3 cut(s) 17, 197, 710
Fsp4HI GCNGC 1 cut(s) 35
GluI GCNGC 1 cut(s) 35
Hin1II CATG 4 cut(s) 34, 130, 171, 181
HinfI GANTC 3 cut(s) 298, 319, 583
HphI GGTGA 1 cut(s) 103
Hpy188I TCNGA 2 cut(s) 598, 635
Hpy188III TCNNGA 2 cut(s) 104, 127
HpyAV CCTTC 2 cut(s) 400, 598
HpyCH4IV ACGT 1 cut(s) 260
HpyCH4V TGCA 4 cut(s) 8, 171, 424, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 223
HpyF3I CTNAG 2 cut(s) 116, 307
HpySE526I ACGT 1 cut(s) 260
Hsp92II CATG 4 cut(s) 34, 130, 171, 181
Kzo9I GATC 1 cut(s) 89
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 3 cut(s) 222, 385, 503
LpnPI CCDG 9 cut(s) 57, 134, 227, 434, 524, 533, 612, 625, 652
MabI ACCWGGT 1 cut(s) 638
MaeII ACGT 1 cut(s) 260
MalI GATC 1 cut(s) 91
MboI GATC 1 cut(s) 89
MboII GAAGA 4 cut(s) 51, 220, 485, 698
MhlI GDGCHC 1 cut(s) 671
MluCI AATT 7 cut(s) 24, 191, 271, 345, 417, 477, 571
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 3 cut(s) 552, 597, 630
Mph1103I ATGCAT 1 cut(s) 173
MseI TTAA 4 cut(s) 137, 288, 644, 675
MslI CAYNNNNRTG 2 cut(s) 176, 182
MspR9I CCNGG 1 cut(s) 640
MvaI CCWGG 1 cut(s) 640
MvnI CGCG 1 cut(s) 79
MwoI GCNNNNNNNGC 1 cut(s) 223
NdeI CATATG 1 cut(s) 660
NdeII GATC 1 cut(s) 89
NlaIII CATG 4 cut(s) 34, 130, 171, 181
NlaIV GGNNCC 3 cut(s) 151, 387, 637
NsiI ATGCAT 1 cut(s) 173
NspV TTCGAA 1 cut(s) 50
PagI TCATGA 1 cut(s) 126
PciSI GCTCTTC 1 cut(s) 233
PfeI GAWTC 3 cut(s) 298, 319, 583
PflMI CCANNNNNTGG 1 cut(s) 448
PkrI GCNGC 1 cut(s) 36
PsiI TTATAA 1 cut(s) 578
Psp1406I AACGTT 1 cut(s) 260
Psp6I CCWGG 1 cut(s) 638
PspGI CCWGG 1 cut(s) 638
PspN4I GGNNCC 3 cut(s) 151, 387, 637
RsaI GTAC 2 cut(s) 353, 377
RsaNI GTAC 2 cut(s) 352, 376
RseI CAYNNNNRTG 2 cut(s) 176, 182
SapI GCTCTTC 1 cut(s) 233
SaqAI TTAA 4 cut(s) 137, 288, 644, 675
SatI GCNGC 1 cut(s) 35
Sau3AI GATC 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 640
SduI GDGCHC 1 cut(s) 671
SexAI ACCWGGT 1 cut(s) 638
SfuI TTCGAA 1 cut(s) 50
SmiMI CAYNNNNRTG 2 cut(s) 176, 182
Sse9I AATT 7 cut(s) 24, 191, 271, 345, 417, 477, 571
SsiI CCGC 3 cut(s) 35, 197, 501
StyD4I CCNGG 1 cut(s) 638
TaiI ACGT 1 cut(s) 263
TaqI TCGA 2 cut(s) 50, 589
TasI AATT 7 cut(s) 24, 191, 271, 345, 417, 477, 571
TatI WGTACW 2 cut(s) 351, 375
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 3 cut(s) 298, 319, 583
Tru1I TTAA 4 cut(s) 137, 288, 644, 675
Tru9I TTAA 4 cut(s) 137, 288, 644, 675
TspDTI ATGAA 6 cut(s) 166, 220, 443, 470, 596, 699
TspGWI ACGGA 1 cut(s) 370
Van91I CCANNNNNTGG 1 cut(s) 448
XagI CCTNNNNNAGG 2 cut(s) 624, 643
XapI RAATTY 1 cut(s) 571
Zsp2I ATGCAT 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.