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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcuI | CTGAAG | 1 cut(s) | 138 |
| AfaI | GTAC | 2 cut(s) | 365, 389 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 319 |
| AgsI | TTSAA | 6 cut(s) | 26, 60, 75, 302, 340, 442 |
| AluBI | AGCT | 3 cut(s) | 56, 521, 554 |
| AluI | AGCT | 3 cut(s) | 56, 521, 554 |
| Alw21I | GWGCWC | 1 cut(s) | 426 |
| AoxI | GGCC | 1 cut(s) | 28 |
| AseI | ATTAAT | 1 cut(s) | 9 |
| AsuHPI | GGTGA | 1 cut(s) | 181 |
| Bbv12I | GWGCWC | 1 cut(s) | 426 |
| BccI | CCATC | 2 cut(s) | 281, 471 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 372, 406 |
| BmiI | GGNNCC | 1 cut(s) | 207 |
| BmrI | ACTGGG | 1 cut(s) | 295 |
| BmuI | ACTGGG | 1 cut(s) | 295 |
| BsaJI | CCNNGG | 1 cut(s) | 201 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 319 |
| Bse1I | ACTGG | 2 cut(s) | 290, 571 |
| Bse3DI | GCAATG | 1 cut(s) | 390 |
| BseDI | CCNNGG | 1 cut(s) | 201 |
| BseGI | GGATG | 2 cut(s) | 482, 493 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 319 |
| BseMI | GCAATG | 1 cut(s) | 390 |
| BseMII | CTCAG | 1 cut(s) | 224 |
| BseNI | ACTGG | 2 cut(s) | 290, 571 |
| BshFI | GGCC | 1 cut(s) | 30 |
| BsiHKAI | GWGCWC | 1 cut(s) | 426 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 319 |
| BsnI | GGCC | 1 cut(s) | 30 |
| Bsp1286I | GDGCHC | 1 cut(s) | 426 |
| Bsp143I | GATC | 1 cut(s) | 492 |
| BspANI | GGCC | 1 cut(s) | 30 |
| BspCNI | CTCAG | 1 cut(s) | 223 |
| BspLI | GGNNCC | 1 cut(s) | 207 |
| BsrDI | GCAATG | 1 cut(s) | 390 |
| BsrI | ACTGG | 2 cut(s) | 290, 571 |
| BssECI | CCNNGG | 1 cut(s) | 201 |
| BssMI | GATC | 1 cut(s) | 492 |
| BssT1I | CCWWGG | 1 cut(s) | 201 |
| Bst4CI | ACNGT | 1 cut(s) | 433 |
| BstC8I | GCNNGC | 1 cut(s) | 176 |
| BstDEI | CTNAG | 3 cut(s) | 210, 218, 603 |
| BstF5I | GGATG | 2 cut(s) | 482, 493 |
| BstKTI | GATC | 1 cut(s) | 495 |
| BstMBI | GATC | 1 cut(s) | 492 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 36 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 202 |
| BsuRI | GGCC | 1 cut(s) | 30 |
| BtsCI | GGATG | 2 cut(s) | 482, 493 |
| Cac8I | GCNNGC | 1 cut(s) | 176 |
| Csp6I | GTAC | 2 cut(s) | 364, 388 |
| CviAII | CATG | 1 cut(s) | 196 |
| CviQI | GTAC | 2 cut(s) | 364, 388 |
| DdeI | CTNAG | 3 cut(s) | 210, 218, 603 |
| DpnI | GATC | 1 cut(s) | 494 |
| DpnII | GATC | 1 cut(s) | 492 |
| Eco130I | CCWWGG | 1 cut(s) | 201 |
| Eco32I | GATATC | 1 cut(s) | 447 |
| Eco57I | CTGAAG | 1 cut(s) | 138 |
| EcoRV | GATATC | 1 cut(s) | 447 |
| EcoT14I | CCWWGG | 1 cut(s) | 201 |
| ErhI | CCWWGG | 1 cut(s) | 201 |
| FaeI | CATG | 1 cut(s) | 199 |
| FaiI | YATR | 8 cut(s) | 84, 98, 197, 347, 417, 500, 559, 561 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 320, 352 |
| FatI | CATG | 1 cut(s) | 195 |
| FokI | GGATG | 2 cut(s) | 489, 500 |
| HaeIII | GGCC | 1 cut(s) | 30 |
| Hin1II | CATG | 1 cut(s) | 199 |
| HinfI | GANTC | 3 cut(s) | 214, 331, 579 |
| HphI | GGTGA | 1 cut(s) | 181 |
| Hpy166II | GTNNAC | 2 cut(s) | 133, 436 |
| Hpy188I | TCNGA | 5 cut(s) | 90, 118, 213, 459, 487 |
| Hpy188III | TCNNGA | 1 cut(s) | 23 |
| Hpy8I | GTNNAC | 2 cut(s) | 133, 436 |
| HpyAV | CCTTC | 2 cut(s) | 20, 308 |
| HpyCH4III | ACNGT | 1 cut(s) | 433 |
| HpyCH4V | TGCA | 2 cut(s) | 383, 502 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 36 |
| HpyF3I | CTNAG | 3 cut(s) | 210, 218, 603 |
| Hsp92II | CATG | 1 cut(s) | 199 |
| Kzo9I | GATC | 1 cut(s) | 492 |
| LmnI | GCTCC | 2 cut(s) | 421, 518 |
| LpnPI | CCDG | 3 cut(s) | 271, 552, 556 |
| MaeIII | GTNAC | 2 cut(s) | 151, 322 |
| MalI | GATC | 1 cut(s) | 494 |
| MboI | GATC | 1 cut(s) | 492 |
| MboII | GAAGA | 1 cut(s) | 454 |
| MhlI | GDGCHC | 1 cut(s) | 426 |
| MluCI | AATT | 6 cut(s) | 6, 70, 126, 141, 250, 526 |
| MlyI | GAGTC | 1 cut(s) | 208 |
| MmeI | TCCRAC | 1 cut(s) | 250 |
| MnlI | CCTC | 5 cut(s) | 84, 146, 202, 219, 545 |
| MseI | TTAA | 3 cut(s) | 9, 255, 525 |
| MslI | CAYNNNNRTG | 1 cut(s) | 194 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 36 |
| NdeII | GATC | 1 cut(s) | 492 |
| NlaIII | CATG | 1 cut(s) | 199 |
| NlaIV | GGNNCC | 1 cut(s) | 207 |
| NmuCI | GTSAC | 1 cut(s) | 322 |
| PfeI | GAWTC | 2 cut(s) | 331, 579 |
| PleI | GAGTC | 1 cut(s) | 208 |
| PpsI | GAGTC | 1 cut(s) | 208 |
| PshBI | ATTAAT | 1 cut(s) | 9 |
| PspN4I | GGNNCC | 1 cut(s) | 207 |
| RsaI | GTAC | 2 cut(s) | 365, 389 |
| RsaNI | GTAC | 2 cut(s) | 364, 388 |
| RseI | CAYNNNNRTG | 1 cut(s) | 194 |
| SaqAI | TTAA | 3 cut(s) | 9, 255, 525 |
| Sau3AI | GATC | 1 cut(s) | 492 |
| SchI | GAGTC | 1 cut(s) | 208 |
| SduI | GDGCHC | 1 cut(s) | 426 |
| SetI | ASST | 5 cut(s) | 58, 138, 194, 523, 556 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 194 |
| Sse9I | AATT | 6 cut(s) | 6, 70, 126, 141, 250, 526 |
| StyI | CCWWGG | 1 cut(s) | 201 |
| TaaI | ACNGT | 1 cut(s) | 433 |
| TaqI | TCGA | 3 cut(s) | 356, 392, 596 |
| TaqII | GACCGA | 1 cut(s) | 310 |
| TasI | AATT | 6 cut(s) | 6, 70, 126, 141, 250, 526 |
| TatI | WGTACW | 1 cut(s) | 387 |
| TfiI | GAWTC | 2 cut(s) | 331, 579 |
| Tru1I | TTAA | 3 cut(s) | 9, 255, 525 |
| Tru9I | TTAA | 3 cut(s) | 9, 255, 525 |
| TseFI | GTSAC | 1 cut(s) | 322 |
| Tsp45I | GTSAC | 1 cut(s) | 322 |
| TspDTI | ATGAA | 1 cut(s) | 113 |
| VspI | ATTAAT | 1 cut(s) | 9 |