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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 169 |
| AccIII | TCCGGA | 1 cut(s) | 22 |
| AfaI | GTAC | 2 cut(s) | 364, 388 |
| AgsI | TTSAA | 3 cut(s) | 16, 60, 75 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 126, 158 |
| AluBI | AGCT | 2 cut(s) | 20, 39 |
| AluI | AGCT | 2 cut(s) | 20, 39 |
| Alw21I | GWGCWC | 1 cut(s) | 314 |
| Alw26I | GTCTC | 1 cut(s) | 328 |
| Aor13HI | TCCGGA | 1 cut(s) | 22 |
| AoxI | GGCC | 1 cut(s) | 28 |
| AspS9I | GGNCC | 1 cut(s) | 29 |
| AsuHPI | GGTGA | 2 cut(s) | 33, 181 |
| Bbv12I | GWGCWC | 1 cut(s) | 314 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 371 |
| BcoDI | GTCTC | 1 cut(s) | 328 |
| BmgT120I | GGNCC | 1 cut(s) | 29 |
| BmiI | GGNNCC | 1 cut(s) | 207 |
| BmrI | ACTGGG | 1 cut(s) | 295 |
| BmuI | ACTGGG | 1 cut(s) | 295 |
| BsaI | GGTCTC | 1 cut(s) | 328 |
| BsaJI | CCNNGG | 1 cut(s) | 201 |
| BsaWI | WCCGGW | 1 cut(s) | 22 |
| Bse1I | ACTGG | 1 cut(s) | 290 |
| Bse3DI | GCAATG | 1 cut(s) | 389 |
| BseAI | TCCGGA | 1 cut(s) | 22 |
| BseDI | CCNNGG | 1 cut(s) | 201 |
| BseMI | GCAATG | 1 cut(s) | 389 |
| BseNI | ACTGG | 1 cut(s) | 290 |
| BshFI | GGCC | 1 cut(s) | 30 |
| BsiHKAI | GWGCWC | 1 cut(s) | 314 |
| BsiSI | CCGG | 1 cut(s) | 23 |
| BslFI | GGGAC | 1 cut(s) | 175 |
| BsmAI | GTCTC | 1 cut(s) | 328 |
| BsmFI | GGGAC | 1 cut(s) | 175 |
| BsnI | GGCC | 1 cut(s) | 30 |
| Bso31I | GGTCTC | 1 cut(s) | 328 |
| Bsp1286I | GDGCHC | 1 cut(s) | 314 |
| Bsp13I | TCCGGA | 1 cut(s) | 22 |
| BspANI | GGCC | 1 cut(s) | 30 |
| BspEI | TCCGGA | 1 cut(s) | 22 |
| BspLI | GGNNCC | 1 cut(s) | 207 |
| BspTNI | GGTCTC | 1 cut(s) | 328 |
| BsrDI | GCAATG | 1 cut(s) | 389 |
| BsrI | ACTGG | 1 cut(s) | 290 |
| BssECI | CCNNGG | 1 cut(s) | 201 |
| BssT1I | CCWWGG | 1 cut(s) | 201 |
| Bst4CI | ACNGT | 3 cut(s) | 127, 166, 331 |
| BstMAI | GTCTC | 1 cut(s) | 328 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 36 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 202 |
| BsuRI | GGCC | 1 cut(s) | 30 |
| Cfr13I | GGNCC | 1 cut(s) | 29 |
| Csp6I | GTAC | 2 cut(s) | 363, 387 |
| CviAII | CATG | 3 cut(s) | 83, 196, 335 |
| CviJI | RGCY | 5 cut(s) | 20, 30, 39, 281, 353 |
| CviKI_1 | RGCY | 5 cut(s) | 20, 30, 39, 281, 353 |
| CviQI | GTAC | 2 cut(s) | 363, 387 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 169 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 169 |
| Eco130I | CCWWGG | 1 cut(s) | 201 |
| Eco31I | GGTCTC | 1 cut(s) | 328 |
| EcoT14I | CCWWGG | 1 cut(s) | 201 |
| ErhI | CCWWGG | 1 cut(s) | 201 |
| FaeI | CATG | 3 cut(s) | 86, 199, 338 |
| FaiI | YATR | 6 cut(s) | 84, 140, 197, 293, 336, 347 |
| FaqI | GGGAC | 1 cut(s) | 175 |
| FatI | CATG | 3 cut(s) | 82, 195, 334 |
| HaeIII | GGCC | 1 cut(s) | 30 |
| HapII | CCGG | 1 cut(s) | 23 |
| Hin1II | CATG | 3 cut(s) | 86, 199, 338 |
| HincII | GTYRAC | 1 cut(s) | 340 |
| HindII | GTYRAC | 1 cut(s) | 340 |
| HinfI | GANTC | 1 cut(s) | 233 |
| HpaII | CCGG | 1 cut(s) | 23 |
| HphI | GGTGA | 2 cut(s) | 33, 181 |
| Hpy166II | GTNNAC | 1 cut(s) | 340 |
| Hpy188III | TCNNGA | 2 cut(s) | 23, 325 |
| Hpy8I | GTNNAC | 1 cut(s) | 340 |
| Hpy99I | CGWCG | 1 cut(s) | 331 |
| HpyAV | CCTTC | 2 cut(s) | 20, 189 |
| HpyCH4III | ACNGT | 3 cut(s) | 127, 166, 331 |
| HpyCH4V | TGCA | 1 cut(s) | 382 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 36 |
| Hsp92II | CATG | 3 cut(s) | 86, 199, 338 |
| Kpn2I | TCCGGA | 1 cut(s) | 22 |
| LmnI | GCTCC | 1 cut(s) | 25 |
| LpnPI | CCDG | 4 cut(s) | 36, 45, 262, 271 |
| MaeIII | GTNAC | 2 cut(s) | 121, 151 |
| MhlI | GDGCHC | 1 cut(s) | 314 |
| MluCI | AATT | 4 cut(s) | 6, 54, 70, 141 |
| MlyI | GAGTC | 1 cut(s) | 227 |
| MmeI | TCCRAC | 2 cut(s) | 157, 250 |
| MnlI | CCTC | 2 cut(s) | 90, 202 |
| MroI | TCCGGA | 1 cut(s) | 22 |
| MseI | TTAA | 3 cut(s) | 9, 255, 300 |
| MslI | CAYNNNNRTG | 1 cut(s) | 194 |
| MspI | CCGG | 1 cut(s) | 23 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 36 |
| NlaIII | CATG | 3 cut(s) | 86, 199, 338 |
| NlaIV | GGNNCC | 1 cut(s) | 207 |
| PleI | GAGTC | 1 cut(s) | 227 |
| PpsI | GAGTC | 1 cut(s) | 227 |
| PspN4I | GGNNCC | 1 cut(s) | 207 |
| PspPI | GGNCC | 1 cut(s) | 29 |
| RsaI | GTAC | 2 cut(s) | 364, 388 |
| RsaNI | GTAC | 2 cut(s) | 363, 387 |
| RseI | CAYNNNNRTG | 1 cut(s) | 194 |
| SaqAI | TTAA | 3 cut(s) | 9, 255, 300 |
| Sau96I | GGNCC | 1 cut(s) | 29 |
| SchI | GAGTC | 1 cut(s) | 227 |
| SduI | GDGCHC | 1 cut(s) | 314 |
| SetI | ASST | 3 cut(s) | 22, 41, 194 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 194 |
| Sse9I | AATT | 4 cut(s) | 6, 54, 70, 141 |
| StyI | CCWWGG | 1 cut(s) | 201 |
| TaaI | ACNGT | 3 cut(s) | 127, 166, 331 |
| TaqI | TCGA | 3 cut(s) | 231, 326, 355 |
| TasI | AATT | 4 cut(s) | 6, 54, 70, 141 |
| TatI | WGTACW | 1 cut(s) | 386 |
| Tru1I | TTAA | 3 cut(s) | 9, 255, 300 |
| Tru9I | TTAA | 3 cut(s) | 9, 255, 300 |
| TspDTI | ATGAA | 2 cut(s) | 113, 323 |