MD12G1254000.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001068
Physical Location & Seq
Forward (+)
32253796 .. 32257203
3408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1254000.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGCTTACGTTCCACCACACAGGCGGCACTCGAAGGGACCGGAGAGGCCATTGCTCAAACCAGAGCTGCTTGCTCCTCAATCCAAGAAAAACTTAAATGTTAAGCCATATAATAAGTCTATTGTGGATTGGACGGAAAATATAGTTTATGCAGACCATTCTACACATCGATGGTGCGCCATCCCTTTGGATGACGAGAACCAGTTTCCATCTTCTGTTAATCTTGAGCCTGTTACCTTGGAGTCTGCTGTGCTGAATTTTGGAGAAAATCATCTAGCTTTGATCAATACTGGTCTAGATAATGGCGGTGAGGTGGAGTGGAACTTGCCAAGGAGCCCTTGGGAATATATAACAGAAAATGTGCTGGAAGACTTGCTTTCGGCATTTAAACACGTGAGGAATGAAATGAAGTCTGCAAGGCCCAAAGAAGTAAGTCCAACTTTGGTTGCCAGAGTGGGGAAAGTACTCTTTCGCAGGAGCCCTTCAATTAACATGGAATCCATCAGAAAAAATTATGGTACTGAAATCTTGCAACAATGGAGGCGATTATTTTACATAAACACTCCTGTTTCATATAAGGGAAAGATTGTGAACGAAGTTGTCCCGAAAATTGGAGTTGATTTCGAAGAGGAGGAAGATACATACGAATTACAGTTGTCTGATTCTACGAGTCCAGAGTCCACTCTCTTCTGCAGATGTCGGGTAATGAAAGAACATGGAAAGCTACAACTCTGTGAGATCAAATTGAAACCAGTGCGTAACATGGTAATGGACATTTCATGCACTACTAAGAATCTGGACCTGAGACTAATGCTGTGCACGGAGAAACTCGTAACTGATCTGACTAATGATGAGATGCAAAGCATCACGGATCTGATTAATTCTGCGATTCTAGATCCAGATTTGAAGGGTGGATTGAGGTGGCCCCCGGGGAAGGAGTCTTCTGGAGATAAATACACAGTTGTTAGGGTTTGTCACGGAATGGGTAGTACGTATAGAAATTCATCACTGGGACTTAAAGTAAAACACTACGATCGATTTGATTTGAGAACCTTAACTGGGGAAGCTTCCTGGGGAATAAGTCTGATGCTGGAAAACGTCGTGTCAAAGTTACAGGAAGAGAATGTTGAAGCCAGCACAATTTCTGAGATACTCAAGGGAGATATGCAGTTGATATGGGATAACTTCTTGAGCTTTGAACGTTTTCTAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

405

Amino Acids

46.2

Weight (kDa)

5.94

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7903 PF25475 44 - 396 4.9e-143 Domain of unknown function (DUF7903)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 25, 306
AclI AACGTT 1 cut(s) 1201
AclWI GGATC 2 cut(s) 879, 890
AcsI RAATTY 2 cut(s) 256, 1000
AcvI CACGTG 1 cut(s) 394
AfaI GTAC 3 cut(s) 465, 520, 991
AfiI CCNNNNNNNGG 4 cut(s) 456, 611, 934, 1059
AflIII ACRYGT 1 cut(s) 391
AgsI TTSAA 5 cut(s) 486, 748, 907, 1130, 1199
AjnI CCWGG 1 cut(s) 1070
AluBI AGCT 5 cut(s) 67, 278, 724, 1067, 1194
AluI AGCT 5 cut(s) 67, 278, 724, 1067, 1194
Alw21I GWGCWC 1 cut(s) 821
Alw26I GTCTC 1 cut(s) 799
Alw44I GTGCAC 1 cut(s) 817
AlwI GGATC 2 cut(s) 879, 890
Ama87I CYCGRG 1 cut(s) 928
AoxI GGCC 3 cut(s) 47, 419, 923
ApaLI GTGCAC 1 cut(s) 817
ApeKI GCWGC 1 cut(s) 67
ApoI RAATTY 2 cut(s) 256, 1000
AseI ATTAAT 1 cut(s) 879
Asp700I GAANNNNTTC 1 cut(s) 1203
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 4 cut(s) 38, 420, 799, 924
AsuC2I CCSGG 2 cut(s) 929, 930
AsuHPI GGTGA 1 cut(s) 320
AsuII TTCGAA 1 cut(s) 624
AvaI CYCGRG 1 cut(s) 928
AvaII GGWCC 2 cut(s) 38, 799
BaeGI GKGCMC 1 cut(s) 821
BanII GRGCYC 2 cut(s) 338, 482
BbrPI CACGTG 1 cut(s) 394
BbsI GAAGAC 2 cut(s) 375, 933
Bbv12I GWGCWC 1 cut(s) 821
BbvI GCAGC 1 cut(s) 54
BccI CCATC 4 cut(s) 165, 188, 217, 509
BciT130I CCWGG 1 cut(s) 1072
BclI TGATCA 1 cut(s) 282
BcnI CCSGG 2 cut(s) 929, 930
BcoDI GTCTC 1 cut(s) 799
BfaI CTAG 3 cut(s) 275, 296, 893
BfmI CTRYAG 1 cut(s) 691
BisI GCNGC 2 cut(s) 26, 68
BlsI GCNGC 2 cut(s) 27, 69
BmcAI AGTACT 1 cut(s) 465
Bme1390I CCNGG 3 cut(s) 929, 930, 1072
Bme18I GGWCC 2 cut(s) 38, 799
BmeT110I CYCGRG 1 cut(s) 928
BmgT120I GGNCC 4 cut(s) 38, 420, 799, 924
BmiI GGNNCC 4 cut(s) 39, 335, 479, 926
BmrFI CCNGG 3 cut(s) 929, 930, 1072
BmrI ACTGGG 2 cut(s) 1019, 1068
BmsI GCATC 3 cut(s) 846, 873, 1077
BmuI ACTGGG 2 cut(s) 1019, 1068
BpiI GAAGAC 2 cut(s) 375, 933
BpmI CTGGAG 1 cut(s) 966
Bpu14I TTCGAA 1 cut(s) 624
BpuEI CTTGAG 3 cut(s) 245, 1139, 1210
BpuMI CCSGG 2 cut(s) 929, 930
Bsa29I ATCGAT 2 cut(s) 169, 1036
BsaAI YACGTR 2 cut(s) 394, 993
BsaJI CCNNGG 7 cut(s) 237, 329, 338, 927, 928, 929, 1071
BsaWI WCCGGW 1 cut(s) 40
Bsc4I CCNNNNNNNGG 4 cut(s) 456, 611, 934, 1059
Bse1I ACTGG 5 cut(s) 202, 295, 752, 1014, 1063
Bse3DI GCAATG 1 cut(s) 50
BseBI CCWGG 1 cut(s) 1072
BseCI ATCGAT 2 cut(s) 169, 1036
BseDI CCNNGG 7 cut(s) 237, 329, 338, 927, 928, 929, 1071
BseGI GGATG 2 cut(s) 180, 196
BseLI CCNNNNNNNGG 4 cut(s) 456, 611, 934, 1059
BseMI GCAATG 1 cut(s) 50
BseMII CTCAG 2 cut(s) 794, 1137
BseNI ACTGG 5 cut(s) 202, 295, 752, 1014, 1063
BseRI GAGGAG 2 cut(s) 66, 644
BseSI GKGCMC 1 cut(s) 821
BseXI GCAGC 1 cut(s) 54
Bsh1285I CGRYCG 1 cut(s) 1036
BshFI GGCC 3 cut(s) 49, 421, 925
BshVI ATCGAT 2 cut(s) 169, 1036
BsiEI CGRYCG 1 cut(s) 1036
BsiHKAI GWGCWC 1 cut(s) 821
BsiHKCI CYCGRG 1 cut(s) 928
BsiSI CCGG 2 cut(s) 41, 929
BslFI GGGAC 3 cut(s) 51, 587, 1026
BslI CCNNNNNNNGG 4 cut(s) 456, 611, 934, 1059
BsmAI GTCTC 1 cut(s) 799
BsmFI GGGAC 3 cut(s) 51, 587, 1026
BsnI GGCC 3 cut(s) 49, 421, 925
BsoBI CYCGRG 1 cut(s) 928
Bsp119I TTCGAA 1 cut(s) 624
Bsp1286I GDGCHC 3 cut(s) 338, 482, 821
Bsp143I GATC 6 cut(s) 282, 738, 838, 871, 895, 1033
BspACI CCGC 2 cut(s) 25, 306
BspANI GGCC 3 cut(s) 49, 421, 925
BspCNI CTCAG 2 cut(s) 795, 1138
BspDI ATCGAT 2 cut(s) 169, 1036
BspLI GGNNCC 4 cut(s) 39, 335, 479, 926
BspMAI CTGCAG 1 cut(s) 695
BspPI GGATC 2 cut(s) 879, 890
BspT104I TTCGAA 1 cut(s) 624
BsrDI GCAATG 1 cut(s) 50
BsrI ACTGG 5 cut(s) 202, 295, 752, 1014, 1063
BssECI CCNNGG 7 cut(s) 237, 329, 338, 927, 928, 929, 1071
BssMI GATC 6 cut(s) 282, 738, 838, 871, 895, 1033
BssT1I CCWWGG 3 cut(s) 237, 329, 338
Bst2UI CCWGG 1 cut(s) 1072
Bst4CI ACNGT 2 cut(s) 654, 961
Bst6I CTCTTC 3 cut(s) 621, 692, 1113
BstBAI YACGTR 2 cut(s) 394, 993
BstBI TTCGAA 1 cut(s) 624
BstC8I GCNNGC 2 cut(s) 72, 1135
BstDEI CTNAG 3 cut(s) 789, 803, 1146
BstF5I GGATG 2 cut(s) 180, 196
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 6 cut(s) 285, 741, 841, 874, 898, 1036
BstMAI GTCTC 1 cut(s) 799
BstMBI GATC 6 cut(s) 282, 738, 838, 871, 895, 1033
BstMCI CGRYCG 1 cut(s) 1036
BstNI CCWGG 1 cut(s) 1072
BstSCI CCNGG 3 cut(s) 927, 928, 1070
BstSFI CTRYAG 1 cut(s) 691
BstSLI GKGCMC 1 cut(s) 821
BstSNI TACGTA 1 cut(s) 993
BstV1I GCAGC 1 cut(s) 54
BstV2I GAAGAC 2 cut(s) 375, 933
BstX2I RGATCY 2 cut(s) 871, 895
BstXI CCANNNNNNTGG 1 cut(s) 187
BstYI RGATCY 2 cut(s) 871, 895
Bsu15I ATCGAT 2 cut(s) 169, 1036
BsuRI GGCC 3 cut(s) 49, 421, 925
BsuTUI ATCGAT 2 cut(s) 169, 1036
BtsCI GGATG 2 cut(s) 180, 196
BtsIMutI CAGTG 2 cut(s) 759, 1007
Cac8I GCNNGC 2 cut(s) 72, 1135
CfoI GCGC 1 cut(s) 179
Cfr13I GGNCC 4 cut(s) 38, 420, 799, 924
Cfr9I CCCGGG 1 cut(s) 928
ClaI ATCGAT 2 cut(s) 169, 1036
Csp6I GTAC 3 cut(s) 464, 519, 990
CviAII CATG 5 cut(s) 493, 716, 763, 780, 1212
CviQI GTAC 3 cut(s) 464, 519, 990
DdeI CTNAG 3 cut(s) 789, 803, 1146
DpnI GATC 6 cut(s) 284, 740, 840, 873, 897, 1035
DpnII GATC 6 cut(s) 282, 738, 838, 871, 895, 1033
DraI TTTAAA 1 cut(s) 388
Eam1104I CTCTTC 3 cut(s) 621, 692, 1113
EarI CTCTTC 3 cut(s) 621, 692, 1113
Eco105I TACGTA 1 cut(s) 993
Eco130I CCWWGG 3 cut(s) 237, 329, 338
Eco24I GRGCYC 2 cut(s) 338, 482
Eco47I GGWCC 2 cut(s) 38, 799
Eco72I CACGTG 1 cut(s) 394
Eco88I CYCGRG 1 cut(s) 928
EcoRII CCWGG 1 cut(s) 1070
EcoT14I CCWWGG 3 cut(s) 237, 329, 338
EcoT38I GRGCYC 2 cut(s) 338, 482
ErhI CCWWGG 3 cut(s) 237, 329, 338
FaeI CATG 5 cut(s) 496, 719, 766, 783, 1215
FalI AAGNNNNNCTT 8 cut(s) 77, 109, 322, 354, 360, 392, 424, 456
FaqI GGGAC 3 cut(s) 51, 587, 1026
FatI CATG 5 cut(s) 492, 715, 762, 779, 1211
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 2 cut(s) 26, 68
FokI GGATG 2 cut(s) 167, 203
FriOI GRGCYC 2 cut(s) 338, 482
Fsp4HI GCNGC 2 cut(s) 26, 68
FspBI CTAG 3 cut(s) 275, 296, 893
GlaI GCGC 1 cut(s) 178
GluI GCNGC 2 cut(s) 26, 68
GsuI CTGGAG 1 cut(s) 966
HaeIII GGCC 3 cut(s) 49, 421, 925
HapII CCGG 2 cut(s) 41, 929
HhaI GCGC 1 cut(s) 179
Hin1II CATG 5 cut(s) 496, 719, 766, 783, 1215
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HindIII AAGCTT 1 cut(s) 1065
HinfI GANTC 8 cut(s) 242, 497, 662, 670, 677, 793, 889, 938
HpaII CCGG 2 cut(s) 41, 929
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 3 cut(s) 592, 681, 819
Hpy188I TCNGA 6 cut(s) 506, 661, 843, 876, 1086, 1147
Hpy188III TCNNGA 9 cut(s) 224, 296, 604, 674, 797, 893, 899, 945, 1189
Hpy8I GTNNAC 3 cut(s) 592, 681, 819
Hpy99I CGWCG 1 cut(s) 1103
HpyAV CCTTC 4 cut(s) 28, 492, 901, 928
HpyCH4III ACNGT 2 cut(s) 654, 961
HpyCH4IV ACGT 5 cut(s) 9, 393, 992, 1098, 1201
HpyCH4V TGCA 8 cut(s) 152, 416, 532, 693, 783, 819, 859, 1168
HpyF3I CTNAG 3 cut(s) 789, 803, 1146
HpySE526I ACGT 5 cut(s) 9, 393, 992, 1098, 1201
Hsp92II CATG 5 cut(s) 496, 719, 766, 783, 1215
HspAI GCGC 1 cut(s) 177
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 6 cut(s) 282, 738, 838, 871, 895, 1033
LmnI GCTCC 3 cut(s) 79, 333, 477
Lsp1109I GCAGC 1 cut(s) 54
LweI GCATC 3 cut(s) 846, 873, 1077
MaeI CTAG 3 cut(s) 275, 296, 893
MaeII ACGT 5 cut(s) 9, 393, 992, 1098, 1201
MaeIII GTNAC 5 cut(s) 232, 758, 832, 974, 1110
MalI GATC 6 cut(s) 284, 740, 840, 873, 897, 1035
MboI GATC 6 cut(s) 282, 738, 838, 871, 895, 1033
MboII GAAGA 7 cut(s) 204, 380, 638, 647, 679, 933, 1130
MflI RGATCY 2 cut(s) 871, 895
MhlI GDGCHC 3 cut(s) 338, 482, 821
MluCI AATT 9 cut(s) 256, 486, 511, 609, 647, 743, 880, 1000, 1140
MlyI GAGTC 4 cut(s) 251, 679, 686, 947
MmeI TCCRAC 1 cut(s) 461
MnlI CCTC 8 cut(s) 39, 87, 304, 390, 534, 622, 625, 912
MroXI GAANNNNTTC 1 cut(s) 1203
MseI TTAA 8 cut(s) 95, 102, 219, 387, 489, 879, 1017, 1055
MslI CAYNNNNRTG 2 cut(s) 169, 767
MspI CCGG 2 cut(s) 41, 929
MspR9I CCNGG 3 cut(s) 929, 930, 1072
MvaI CCWGG 1 cut(s) 1072
NciI CCSGG 2 cut(s) 929, 930
NdeII GATC 6 cut(s) 282, 738, 838, 871, 895, 1033
NlaIII CATG 5 cut(s) 496, 719, 766, 783, 1215
NlaIV GGNNCC 4 cut(s) 39, 335, 479, 926
NmuCI GTSAC 1 cut(s) 974
NspV TTCGAA 1 cut(s) 624
PdmI GAANNNNTTC 1 cut(s) 1203
PfeI GAWTC 4 cut(s) 497, 662, 793, 889
PkrI GCNGC 2 cut(s) 27, 69
Ple19I CGATCG 1 cut(s) 1036
PleI GAGTC 4 cut(s) 250, 678, 685, 946
PmaCI CACGTG 1 cut(s) 394
PmlI CACGTG 1 cut(s) 394
PpsI GAGTC 4 cut(s) 250, 678, 685, 946
Ppu21I YACGTR 2 cut(s) 394, 993
PshBI ATTAAT 1 cut(s) 879
Psp1406I AACGTT 1 cut(s) 1201
Psp6I CCWGG 1 cut(s) 1070
PspCI CACGTG 1 cut(s) 394
PspGI CCWGG 1 cut(s) 1070
PspN4I GGNNCC 4 cut(s) 39, 335, 479, 926
PspPI GGNCC 4 cut(s) 38, 420, 799, 924
PstI CTGCAG 1 cut(s) 695
PsuI RGATCY 2 cut(s) 871, 895
PvuI CGATCG 1 cut(s) 1036
RsaI GTAC 3 cut(s) 465, 520, 991
RsaNI GTAC 3 cut(s) 464, 519, 990
RseI CAYNNNNRTG 2 cut(s) 169, 767
SaqAI TTAA 8 cut(s) 95, 102, 219, 387, 489, 879, 1017, 1055
SatI GCNGC 2 cut(s) 26, 68
Sau3AI GATC 6 cut(s) 282, 738, 838, 871, 895, 1033
Sau96I GGNCC 4 cut(s) 38, 420, 799, 924
ScaI AGTACT 1 cut(s) 465
SchI GAGTC 4 cut(s) 251, 679, 686, 947
ScrFI CCNGG 3 cut(s) 929, 930, 1072
SduI GDGCHC 3 cut(s) 338, 482, 821
SfaNI GCATC 3 cut(s) 846, 873, 1077
SfcI CTRYAG 1 cut(s) 691
SfuI TTCGAA 1 cut(s) 624
SinI GGWCC 2 cut(s) 38, 799
SmaI CCCGGG 1 cut(s) 930
SmiMI CAYNNNNRTG 2 cut(s) 169, 767
SmlI CTYRAG 3 cut(s) 224, 1154, 1189
SmoI CTYRAG 3 cut(s) 224, 1154, 1189
SnaBI TACGTA 1 cut(s) 993
Sse9I AATT 9 cut(s) 256, 486, 511, 609, 647, 743, 880, 1000, 1140
SsiI CCGC 2 cut(s) 25, 306
SspMI CTAG 3 cut(s) 275, 296, 893
StyD4I CCNGG 3 cut(s) 927, 928, 1070
StyI CCWWGG 3 cut(s) 237, 329, 338
TaaI ACNGT 2 cut(s) 654, 961
TaiI ACGT 5 cut(s) 12, 396, 995, 1101, 1204
TaqI TCGA 4 cut(s) 32, 169, 624, 1036
TasI AATT 9 cut(s) 256, 486, 511, 609, 647, 743, 880, 1000, 1140
TatI WGTACW 1 cut(s) 463
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 4 cut(s) 497, 662, 793, 889
Tru1I TTAA 8 cut(s) 95, 102, 219, 387, 489, 879, 1017, 1055
Tru9I TTAA 8 cut(s) 95, 102, 219, 387, 489, 879, 1017, 1055
TscAI CASTG 2 cut(s) 759, 1014
TseFI GTSAC 1 cut(s) 974
TseI GCWGC 1 cut(s) 67
Tsp45I GTSAC 1 cut(s) 974
TspDTI ATGAA 6 cut(s) 417, 422, 561, 722, 768, 993
TspGWI ACGGA 4 cut(s) 149, 836, 884, 993
TspMI CCCGGG 1 cut(s) 928
TspRI CASTG 2 cut(s) 759, 1014
VneI GTGCAC 1 cut(s) 817
VpaK11BI GGWCC 2 cut(s) 38, 799
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 2 cut(s) 256, 1000
XbaI TCTAGA 2 cut(s) 295, 892
XcmI CCANNNNNNNNNTGG 1 cut(s) 336
XmaI CCCGGG 1 cut(s) 928
XmnI GAANNNNTTC 1 cut(s) 1203
XspI CTAG 3 cut(s) 275, 296, 893
ZrmI AGTACT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.