MD12G1254400.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001068
Physical Location & Seq
Forward (+)
32279769 .. 32283580
3812 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1254400.v1.1.491

Sequence Viewer

Length: 1290 bp
ATGTCTACAAAACCTCACAAGGCAATGCAGAGATTTCTATCTGCCTTTCTGGGCATAAGTGCTAGTTCTACAATGGCTTACGTTCCACCACACAGGCGGCACTCGAAGGAATCGGAGAGGCCGTTGCCAACTCCAGAGCTGCTTGCTCCTCAATTCAAGAAAAACTCAAATGACAAGCAATATAATAAGTCTAATGTGGGTTGGAGTGGAAAAGTAGTTTATGCAGACAATTCTACTCATAGATGGTGCGCCATCCCTTTGGATGACGAGAACCAGTTTCCATCTTTTGTTAATCTTGAGCCCGTTGCCTTGGAGTCTGCTGAGCAGACAATTGGAAAAAATCGTGTAGCTCTGATCAATACTAGTCTAAATAATGAAGGCAGTGAGGTGAAATGGAACTTGCCAAGGAGCCCTTGGGAATCTATAACAGAAAACGTGCTCGAAGACTTACTTTCGGCATTTAAAGACATGAGGAATGAAATGAAGTCTGCGAAGTCCAAAGAAGAATATCCAACTTTGGTTGCCAAAGTGGGGAAAGTACTCTTTCACAGGAGCCCTTCAGTTAACGGGGCATCCATCAGAAAAGAATTGTGCACTGAAATCTTGAAACAATGGCGGCGATCATTTTACACAAACACTCCTGTTTCATATAAGGAAAGGATTGTCAACGAAGTTGTCCCAAAAATTGGAGTTGATTTCAAAGATGAAGAAGATATATACGAAGTACAGTTGTCTGATTCTACGAAGCCAGATTTACCTTTCTCCTGCAAATGTCGGGTAATGGAAGAACATGGAAAGCTACAACCCTGCACGATCAAATTCAATCCAGTGCGTAACATGGTAATGGACATTTCATGCACTACTAAGAATCTGGACCTGAGGTTAATGCTATGCACGAAGAAACTCGTAACTGATCTGACTGATGATGAGATGCAAAGCATTACGGATCTGATTAATTCTGCAATTCTAGATCCAAATGTGAAGGGTGGATTGAGATGGCCCCTGGGGAAGGAGTCTTCTGGAGATAGATACAGAGTTGTTAGGGTTTGGCACGTAATAGCTAATACGTATAGAAATTCATCACTGACTCTGAAAGTGAAACACACTGATCGATTTGATTTTAGAACCTTAACTGGAGAAACTTCTTGGTGGACAAGTCTGGTGCTGGAAAACGTCGTGTCAAAGTTACAGGAAGAGAATGTGGAAGCCAGCACAATTTCTGAGATACTCAAGGAAGATATGCAGTTGATATGGGAAAACTTCTTGAGCTGTGAACCTTTTCTAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

430

Amino Acids

49.12

Weight (kDa)

7.08

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7903 PF25475 68 - 421 4.6e-149 Domain of unknown function (DUF7903)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 686
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 97, 616
AclWI GGATC 2 cut(s) 954, 965
AcsI RAATTY 2 cut(s) 818, 1075
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 2 cut(s) 540, 726
AfiI CCNNNNNNNGG 3 cut(s) 531, 686, 1009
AgsI TTSAA 4 cut(s) 157, 607, 700, 823
AhlI ACTAGT 1 cut(s) 362
AjnI CCWGG 1 cut(s) 1002
AluBI AGCT 5 cut(s) 139, 350, 799, 1061, 1269
AluI AGCT 5 cut(s) 139, 350, 799, 1061, 1269
Alw21I GWGCWC 2 cut(s) 441, 596
Alw44I GTGCAC 1 cut(s) 592
AlwI GGATC 2 cut(s) 954, 965
AoxI GGCC 2 cut(s) 119, 998
ApaLI GTGCAC 1 cut(s) 592
ApeKI GCWGC 1 cut(s) 139
ApoI RAATTY 2 cut(s) 818, 1075
AseI ATTAAT 1 cut(s) 954
Asp700I GAANNNNTTC 1 cut(s) 1278
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 2 cut(s) 874, 999
AsuHPI GGTGA 1 cut(s) 400
AvaII GGWCC 1 cut(s) 874
AxyI CCTNAGG 1 cut(s) 878
BaeGI GKGCMC 1 cut(s) 596
BanII GRGCYC 3 cut(s) 303, 413, 557
BbsI GAAGAC 2 cut(s) 450, 1008
Bbv12I GWGCWC 2 cut(s) 441, 596
BbvI GCAGC 1 cut(s) 126
BccI CCATC 5 cut(s) 237, 260, 289, 584, 990
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 1004
BclI TGATCA 1 cut(s) 354
BcuI ACTAGT 1 cut(s) 362
BfaI CTAG 3 cut(s) 63, 363, 968
BisI GCNGC 3 cut(s) 98, 140, 617
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 3 cut(s) 99, 141, 618
BmcAI AGTACT 1 cut(s) 540
Bme1390I CCNGG 1 cut(s) 1004
Bme18I GGWCC 1 cut(s) 874
BmgT120I GGNCC 2 cut(s) 874, 999
BmiI GGNNCC 3 cut(s) 410, 554, 1001
BmrFI CCNGG 1 cut(s) 1004
BmsI GCATC 2 cut(s) 581, 921
BpiI GAAGAC 2 cut(s) 450, 1008
BpmI CTGGAG 3 cut(s) 117, 1041, 1155
Bpu1102I GCTNAGC 1 cut(s) 321
BpuEI CTTGAG 3 cut(s) 317, 1214, 1285
Bsa29I ATCGAT 1 cut(s) 1111
BsaAI YACGTR 2 cut(s) 1054, 1068
BsaBI GATNNNNATC 1 cut(s) 37
BsaJI CCNNGG 5 cut(s) 309, 404, 413, 1002, 1003
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 622, 652
Bsc4I CCNNNNNNNGG 3 cut(s) 531, 686, 1009
Bse1I ACTGG 3 cut(s) 274, 827, 1138
Bse21I CCTNAGG 1 cut(s) 878
Bse3DI GCAATG 1 cut(s) 30
Bse8I GATNNNNATC 1 cut(s) 37
BseBI CCWGG 1 cut(s) 1004
BseCI ATCGAT 1 cut(s) 1111
BseDI CCNNGG 5 cut(s) 309, 404, 413, 1002, 1003
BseGI GGATG 3 cut(s) 252, 268, 572
BseJI GATNNNNATC 1 cut(s) 37
BseLI CCNNNNNNNGG 3 cut(s) 531, 686, 1009
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 3 cut(s) 312, 869, 1212
BseNI ACTGG 3 cut(s) 274, 827, 1138
BseRI GAGGAG 1 cut(s) 138
BseSI GKGCMC 1 cut(s) 596
BseXI GCAGC 1 cut(s) 126
BsgI GTGCAG 1 cut(s) 793
BshFI GGCC 2 cut(s) 121, 1000
BshVI ATCGAT 1 cut(s) 1111
BsiHKAI GWGCWC 2 cut(s) 441, 596
BslFI GGGAC 1 cut(s) 662
BslI CCNNNNNNNGG 3 cut(s) 531, 686, 1009
BsmFI GGGAC 1 cut(s) 662
BsnI GGCC 2 cut(s) 121, 1000
Bsp1286I GDGCHC 5 cut(s) 303, 413, 441, 557, 596
Bsp143I GATC 7 cut(s) 354, 620, 813, 913, 946, 970, 1108
Bsp1720I GCTNAGC 1 cut(s) 321
BspACI CCGC 2 cut(s) 97, 616
BspANI GGCC 2 cut(s) 121, 1000
BspCNI CTCAG 3 cut(s) 313, 870, 1213
BspDI ATCGAT 1 cut(s) 1111
BspLI GGNNCC 3 cut(s) 410, 554, 1001
BspPI GGATC 2 cut(s) 954, 965
BsrDI GCAATG 1 cut(s) 30
BsrI ACTGG 3 cut(s) 274, 827, 1138
BssECI CCNNGG 5 cut(s) 309, 404, 413, 1002, 1003
BssMI GATC 7 cut(s) 354, 620, 813, 913, 946, 970, 1108
BssT1I CCWWGG 3 cut(s) 309, 404, 413
Bst2UI CCWGG 1 cut(s) 1004
Bst4CI ACNGT 1 cut(s) 729
Bst6I CTCTTC 1 cut(s) 1188
BstBAI YACGTR 2 cut(s) 1054, 1068
BstC8I GCNNGC 2 cut(s) 144, 1210
BstDEI CTNAG 4 cut(s) 321, 864, 878, 1221
BstENI CCTNNNNNAGG 1 cut(s) 1007
BstF5I GGATG 3 cut(s) 252, 268, 572
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 7 cut(s) 357, 623, 816, 916, 949, 973, 1111
BstMBI GATC 7 cut(s) 354, 620, 813, 913, 946, 970, 1108
BstNI CCWGG 1 cut(s) 1004
BstSCI CCNGG 1 cut(s) 1002
BstSLI GKGCMC 1 cut(s) 596
BstSNI TACGTA 1 cut(s) 1068
BstV1I GCAGC 1 cut(s) 126
BstV2I GAAGAC 2 cut(s) 450, 1008
BstX2I RGATCY 2 cut(s) 946, 970
BstXI CCANNNNNNTGG 1 cut(s) 259
BstYI RGATCY 2 cut(s) 946, 970
Bsu15I ATCGAT 1 cut(s) 1111
Bsu36I CCTNAGG 1 cut(s) 878
BsuRI GGCC 2 cut(s) 121, 1000
BsuTUI ATCGAT 1 cut(s) 1111
BtsCI GGATG 3 cut(s) 252, 268, 572
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 5 cut(s) 388, 594, 834, 1082, 1104
Cac8I GCNNGC 2 cut(s) 144, 1210
CfoI GCGC 1 cut(s) 251
Cfr13I GGNCC 2 cut(s) 874, 999
ClaI ATCGAT 1 cut(s) 1111
Csp6I GTAC 2 cut(s) 539, 725
CviAII CATG 5 cut(s) 469, 791, 838, 855, 1287
CviQI GTAC 2 cut(s) 539, 725
DdeI CTNAG 4 cut(s) 321, 864, 878, 1221
DpnI GATC 7 cut(s) 356, 622, 815, 915, 948, 972, 1110
DpnII GATC 7 cut(s) 354, 620, 813, 913, 946, 970, 1108
DraI TTTAAA 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 1188
EarI CTCTTC 1 cut(s) 1188
Eco105I TACGTA 1 cut(s) 1068
Eco130I CCWWGG 3 cut(s) 309, 404, 413
Eco24I GRGCYC 3 cut(s) 303, 413, 557
Eco47I GGWCC 1 cut(s) 874
Eco57I CTGAAG 1 cut(s) 543
Eco81I CCTNAGG 1 cut(s) 878
EcoNI CCTNNNNNAGG 1 cut(s) 1007
EcoRII CCWGG 1 cut(s) 1002
EcoT14I CCWWGG 3 cut(s) 309, 404, 413
EcoT38I GRGCYC 3 cut(s) 303, 413, 557
ErhI CCWWGG 3 cut(s) 309, 404, 413
FaeI CATG 5 cut(s) 472, 794, 841, 858, 1290
FalI AAGNNNNNCTT 4 cut(s) 397, 429, 435, 467
FaqI GGGAC 1 cut(s) 662
FatI CATG 5 cut(s) 468, 790, 837, 854, 1286
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 98, 140, 617
FokI GGATG 3 cut(s) 239, 275, 559
FriOI GRGCYC 3 cut(s) 303, 413, 557
Fsp4HI GCNGC 3 cut(s) 98, 140, 617
FspBI CTAG 3 cut(s) 63, 363, 968
GlaI GCGC 1 cut(s) 250
GluI GCNGC 3 cut(s) 98, 140, 617
GsuI CTGGAG 3 cut(s) 117, 1041, 1155
HaeIII GGCC 2 cut(s) 121, 1000
HhaI GCGC 1 cut(s) 251
Hin1II CATG 5 cut(s) 472, 794, 841, 858, 1290
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HincII GTYRAC 2 cut(s) 565, 667
HindII GTYRAC 2 cut(s) 565, 667
HinfI GANTC 7 cut(s) 110, 314, 419, 737, 868, 1013, 1087
HpaI GTTAAC 1 cut(s) 565
HphI GGTGA 1 cut(s) 400
Hpy166II GTNNAC 6 cut(s) 6, 565, 594, 667, 1152, 1274
Hpy188I TCNGA 8 cut(s) 115, 354, 581, 736, 918, 951, 1092, 1222
Hpy188III TCNNGA 8 cut(s) 134, 157, 296, 604, 872, 968, 1020, 1264
Hpy8I GTNNAC 6 cut(s) 6, 565, 594, 667, 1152, 1274
Hpy99I CGWCG 1 cut(s) 1178
HpyAV CCTTC 5 cut(s) 100, 371, 567, 976, 1003
HpyCH4III ACNGT 1 cut(s) 729
HpyCH4IV ACGT 5 cut(s) 81, 435, 1053, 1067, 1173
HpyF3I CTNAG 4 cut(s) 321, 864, 878, 1221
HpySE526I ACGT 5 cut(s) 81, 435, 1053, 1067, 1173
Hsp92II CATG 5 cut(s) 472, 794, 841, 858, 1290
HspAI GCGC 1 cut(s) 249
Ksp22I TGATCA 1 cut(s) 354
KspAI GTTAAC 1 cut(s) 565
Kzo9I GATC 7 cut(s) 354, 620, 813, 913, 946, 970, 1108
LmnI GCTCC 3 cut(s) 151, 408, 552
Lsp1109I GCAGC 1 cut(s) 126
LweI GCATC 2 cut(s) 581, 921
MaeI CTAG 3 cut(s) 63, 363, 968
MaeII ACGT 5 cut(s) 81, 435, 1053, 1067, 1173
MaeIII GTNAC 3 cut(s) 833, 907, 1185
MalI GATC 7 cut(s) 356, 622, 815, 915, 948, 972, 1110
MboI GATC 7 cut(s) 354, 620, 813, 913, 946, 970, 1108
MboII GAAGA 9 cut(s) 455, 515, 719, 722, 797, 910, 1008, 1205, 1247
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 2 cut(s) 946, 970
MhlI GDGCHC 5 cut(s) 303, 413, 441, 557, 596
MlyI GAGTC 3 cut(s) 323, 1022, 1081
MmeI TCCRAC 2 cut(s) 182, 536
MnlI CCTC 6 cut(s) 24, 111, 159, 379, 465, 873
MroXI GAANNNNTTC 1 cut(s) 1278
MseI TTAA 6 cut(s) 291, 462, 564, 884, 954, 1130
MslI CAYNNNNRTG 1 cut(s) 842
MspR9I CCNGG 1 cut(s) 1004
MunI CAATTG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 1004
NdeII GATC 7 cut(s) 354, 620, 813, 913, 946, 970, 1108
NlaIII CATG 5 cut(s) 472, 794, 841, 858, 1290
NlaIV GGNNCC 3 cut(s) 410, 554, 1001
PasI CCCWGGG 1 cut(s) 1003
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 1278
PfeI GAWTC 4 cut(s) 110, 419, 737, 868
PflMI CCANNNNNTGG 1 cut(s) 686
PkrI GCNGC 3 cut(s) 99, 141, 618
PleI GAGTC 3 cut(s) 322, 1021, 1081
PpsI GAGTC 3 cut(s) 322, 1021, 1081
Ppu21I YACGTR 2 cut(s) 1054, 1068
PshBI ATTAAT 1 cut(s) 954
Psp6I CCWGG 1 cut(s) 1002
PspGI CCWGG 1 cut(s) 1002
PspN4I GGNNCC 3 cut(s) 410, 554, 1001
PspPI GGNCC 2 cut(s) 874, 999
PsuI RGATCY 2 cut(s) 946, 970
RsaI GTAC 2 cut(s) 540, 726
RsaNI GTAC 2 cut(s) 539, 725
RseI CAYNNNNRTG 1 cut(s) 842
SaqAI TTAA 6 cut(s) 291, 462, 564, 884, 954, 1130
SatI GCNGC 3 cut(s) 98, 140, 617
Sau3AI GATC 7 cut(s) 354, 620, 813, 913, 946, 970, 1108
Sau96I GGNCC 2 cut(s) 874, 999
ScaI AGTACT 1 cut(s) 540
SchI GAGTC 3 cut(s) 323, 1022, 1081
ScrFI CCNGG 1 cut(s) 1004
SduI GDGCHC 5 cut(s) 303, 413, 441, 557, 596
SfaNI GCATC 2 cut(s) 581, 921
SinI GGWCC 1 cut(s) 874
SmiMI CAYNNNNRTG 1 cut(s) 842
SmlI CTYRAG 3 cut(s) 296, 1229, 1264
SmoI CTYRAG 3 cut(s) 296, 1229, 1264
SnaBI TACGTA 1 cut(s) 1068
SpeI ACTAGT 1 cut(s) 362
SsiI CCGC 2 cut(s) 97, 616
SspMI CTAG 3 cut(s) 63, 363, 968
StyD4I CCNGG 1 cut(s) 1002
StyI CCWWGG 3 cut(s) 309, 404, 413
TaaI ACNGT 1 cut(s) 729
TaiI ACGT 5 cut(s) 84, 438, 1056, 1070, 1176
TaqI TCGA 3 cut(s) 104, 441, 1111
TatI WGTACW 2 cut(s) 538, 724
TauI GCSGC 2 cut(s) 100, 619
TfiI GAWTC 4 cut(s) 110, 419, 737, 868
Tru1I TTAA 6 cut(s) 291, 462, 564, 884, 954, 1130
Tru9I TTAA 6 cut(s) 291, 462, 564, 884, 954, 1130
TscAI CASTG 5 cut(s) 388, 601, 834, 1089, 1111
TseI GCWGC 1 cut(s) 139
TspDTI ATGAA 7 cut(s) 390, 492, 497, 636, 720, 843, 1068
TspGWI ACGGA 1 cut(s) 959
TspRI CASTG 5 cut(s) 388, 601, 834, 1089, 1111
Van91I CCANNNNNTGG 1 cut(s) 686
VneI GTGCAC 1 cut(s) 592
VpaK11BI GGWCC 1 cut(s) 874
VspI ATTAAT 1 cut(s) 954
XagI CCTNNNNNAGG 1 cut(s) 1007
XapI RAATTY 2 cut(s) 818, 1075
XbaI TCTAGA 1 cut(s) 967
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 1278
XspI CTAG 3 cut(s) 63, 363, 968
ZrmI AGTACT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.