pycom12g23300

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001068
Physical Location & Seq
Forward (+)
23939460 .. 23940867
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g23300.3

Sequence Viewer

Length: 633 bp
ATGAAATTTTTGAAGTTTCTTTTTGCCTTGCGTCCCACACCACAAGCGGCAGTTAAAGGAAAAGGAATTTTGATGCATGATTATGCAAACACATTGATAATAGGTGGAGTATATATACTCTCGAGGTTCTATAGGGGAAAATCGGTAAATCTCTCTCCTTTGTTCCTTGTCCTTGTATGGTTGTCAGACTCTAGACGACCAAAATCGATCATCACTTGCAAATGCAGTGTAGCAAAAGAACATGGAAAGCTACAACTCTACAAGGATGATGAGATGCAAAGCATTGGGGATGTAATTAGTTTGGCGATTCAGGATTCAAATGTGAAGGGAGGGTTGAGATGGCCCCTAGGGAAGGCGTTTTCTGGAGGCCGGTATAAGGTAGTTGTAATTTGGCATACAATTACCACTACTTATGAAAATCCATCTCTAAAACTGAAGTTAAGGCATGCAAATCGCTTCGATTTTACCACCTCAGCTGGTGAGACTGGTTGGGAGGTCAGGCTGTTTCTGCAAATGCTCATTTCAAAGTTACAGGAAGAGAATGTTGAAGTCGGCTTGATTTCTGATATGCTGCAGGAAAATATGAAGTTGATATGGGACGACTTCTTATGCTGCGAACCTTTTCCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

211

Amino Acids

23.89

Weight (kDa)

9.25

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 47
AcsI RAATTY 2 cut(s) 5, 66
AcuI CTGAAG 1 cut(s) 455
AfiI CCNNNNNNNGG 2 cut(s) 352, 376
AgsI TTSAA 4 cut(s) 13, 318, 525, 548
AluBI AGCT 2 cut(s) 250, 476
AluI AGCT 2 cut(s) 250, 476
Alw26I GTCTC 1 cut(s) 476
Ama87I CYCGRG 1 cut(s) 121
AoxI GGCC 2 cut(s) 341, 367
ApeKI GCWGC 2 cut(s) 571, 612
ApoI RAATTY 2 cut(s) 5, 66
Asp700I GAANNNNTTC 1 cut(s) 621
AspA2I CCTAGG 1 cut(s) 346
AspS9I GGNCC 1 cut(s) 342
AsuHPI GGTGA 1 cut(s) 491
AvaI CYCGRG 1 cut(s) 121
AvrII CCTAGG 1 cut(s) 346
BbvCI CCTCAGC 1 cut(s) 472
BbvI GCAGC 2 cut(s) 558, 599
BccI CCATC 2 cut(s) 333, 430
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 2 cut(s) 192, 347
BfmI CTRYAG 2 cut(s) 130, 572
BisI GCNGC 3 cut(s) 48, 572, 613
BlnI CCTAGG 1 cut(s) 346
BlsI GCNGC 3 cut(s) 49, 573, 614
BmeT110I CYCGRG 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 342
BmiI GGNNCC 1 cut(s) 344
BmsI GCATC 2 cut(s) 63, 264
BpmI CTGGAG 1 cut(s) 384
Bpu10I CCTNAGC 1 cut(s) 472
Bsa29I ATCGAT 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 346
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 376
Bse118I RCCGGY 1 cut(s) 369
Bse1I ACTGG 1 cut(s) 490
BseCI ATCGAT 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 2 cut(s) 271, 295
BseLI CCNNNNNNNGG 2 cut(s) 352, 376
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 1 cut(s) 490
BseXI GCAGC 2 cut(s) 558, 599
BshFI GGCC 2 cut(s) 343, 369
BshVI ATCGAT 1 cut(s) 206
BsiHKCI CYCGRG 1 cut(s) 121
BsiSI CCGG 1 cut(s) 370
BslFI GGGAC 2 cut(s) 18, 611
BslI CCNNNNNNNGG 2 cut(s) 352, 376
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 2 cut(s) 18, 611
BsnI GGCC 2 cut(s) 343, 369
BsoBI CYCGRG 1 cut(s) 121
Bsp143I GATC 1 cut(s) 207
BspACI CCGC 1 cut(s) 47
BspANI GGCC 2 cut(s) 343, 369
BspCNI CTCAG 1 cut(s) 485
BspDI ATCGAT 1 cut(s) 206
BspLI GGNNCC 1 cut(s) 344
BspMAI CTGCAG 1 cut(s) 576
BsrFI RCCGGY 1 cut(s) 369
BsrI ACTGG 1 cut(s) 490
BssAI RCCGGY 1 cut(s) 369
BssECI CCNNGG 1 cut(s) 346
BssMI GATC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 346
Bst6I CTCTTC 1 cut(s) 531
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 1 cut(s) 472
BstENI CCTNNNNNAGG 1 cut(s) 350
BstF5I GGATG 2 cut(s) 271, 295
BstKTI GATC 1 cut(s) 210
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 1 cut(s) 207
BstMWI GCNNNNNNNGC 1 cut(s) 508
BstNSI RCATGY 1 cut(s) 449
BstSFI CTRYAG 2 cut(s) 130, 572
BstV1I GCAGC 2 cut(s) 558, 599
Bsu15I ATCGAT 1 cut(s) 206
BsuRI GGCC 2 cut(s) 343, 369
BsuTUI ATCGAT 1 cut(s) 206
BtsCI GGATG 2 cut(s) 271, 295
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 369
Cfr13I GGNCC 1 cut(s) 342
ClaI ATCGAT 1 cut(s) 206
CseI GACGC 1 cut(s) 20
CviAII CATG 4 cut(s) 77, 242, 446, 630
CviJI RGCY 6 cut(s) 250, 343, 369, 476, 502, 555
CviKI_1 RGCY 6 cut(s) 250, 343, 369, 476, 502, 555
DdeI CTNAG 1 cut(s) 472
DpnI GATC 1 cut(s) 209
DpnII GATC 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
Eco130I CCWWGG 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 455
Eco88I CYCGRG 1 cut(s) 121
EcoNI CCTNNNNNAGG 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 346
EcoT22I ATGCAT 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 346
FaeI CATG 4 cut(s) 80, 245, 449, 633
FaqI GGGAC 2 cut(s) 18, 611
FatI CATG 4 cut(s) 76, 241, 445, 629
Fnu4HI GCNGC 3 cut(s) 48, 572, 613
FokI GGATG 2 cut(s) 278, 302
Fsp4HI GCNGC 3 cut(s) 48, 572, 613
FspBI CTAG 2 cut(s) 192, 347
GluI GCNGC 3 cut(s) 48, 572, 613
GsuI CTGGAG 1 cut(s) 384
HaeIII GGCC 2 cut(s) 343, 369
HapII CCGG 1 cut(s) 370
HgaI GACGC 1 cut(s) 20
Hin1II CATG 4 cut(s) 80, 245, 449, 633
HinfI GANTC 3 cut(s) 188, 307, 314
HpaII CCGG 1 cut(s) 370
HphI GGTGA 1 cut(s) 491
Hpy188I TCNGA 2 cut(s) 187, 565
Hpy188III TCNNGA 4 cut(s) 121, 192, 311, 363
HpyAV CCTTC 2 cut(s) 319, 346
HpyCH4V TGCA 8 cut(s) 76, 86, 219, 225, 277, 449, 511, 574
HpyF10VI GCNNNNNNNGC 1 cut(s) 508
HpyF3I CTNAG 1 cut(s) 472
Hsp92II CATG 4 cut(s) 80, 245, 449, 633
Kzo9I GATC 1 cut(s) 207
LpnPI CCDG 8 cut(s) 296, 348, 383, 462, 471, 484, 518, 560
Lsp1109I GCAGC 2 cut(s) 558, 599
LweI GCATC 2 cut(s) 63, 264
MaeI CTAG 2 cut(s) 192, 347
MaeIII GTNAC 1 cut(s) 528
MalI GATC 1 cut(s) 209
MboI GATC 1 cut(s) 207
MboII GAAGA 1 cut(s) 548
MluCI AATT 5 cut(s) 5, 66, 294, 387, 399
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 5 cut(s) 117, 323, 359, 481, 487
Mph1103I ATGCAT 1 cut(s) 78
MroXI GAANNNNTTC 1 cut(s) 621
MseI TTAA 2 cut(s) 54, 440
MslI CAYNNNNRTG 1 cut(s) 81
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 1 cut(s) 370
MwoI GCNNNNNNNGC 1 cut(s) 508
NdeII GATC 1 cut(s) 207
NlaIII CATG 4 cut(s) 80, 245, 449, 633
NlaIV GGNNCC 1 cut(s) 344
NsiI ATGCAT 1 cut(s) 78
NspI RCATGY 1 cut(s) 449
PaeI GCATGC 1 cut(s) 449
PaeR7I CTCGAG 1 cut(s) 121
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 2 cut(s) 307, 314
PkrI GCNGC 3 cut(s) 49, 573, 614
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
PspN4I GGNNCC 1 cut(s) 344
PspPI GGNCC 1 cut(s) 342
PstI CTGCAG 1 cut(s) 576
PvuII CAGCTG 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 81
SaqAI TTAA 2 cut(s) 54, 440
SatI GCNGC 3 cut(s) 48, 572, 613
Sau3AI GATC 1 cut(s) 207
Sau96I GGNCC 1 cut(s) 342
SchI GAGTC 1 cut(s) 182
SetI ASST 8 cut(s) 106, 128, 252, 381, 473, 478, 498, 622
SfaNI GCATC 2 cut(s) 63, 264
SfcI CTRYAG 2 cut(s) 130, 572
Sfr274I CTCGAG 1 cut(s) 121
SlaI CTCGAG 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 81
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
SphI GCATGC 1 cut(s) 449
Sse9I AATT 5 cut(s) 5, 66, 294, 387, 399
SsiI CCGC 1 cut(s) 47
SspMI CTAG 2 cut(s) 192, 347
StyI CCWWGG 1 cut(s) 346
TaqI TCGA 3 cut(s) 122, 206, 459
TasI AATT 5 cut(s) 5, 66, 294, 387, 399
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 2 cut(s) 307, 314
Tru1I TTAA 2 cut(s) 54, 440
Tru9I TTAA 2 cut(s) 54, 440
TscAI CASTG 1 cut(s) 232
TseI GCWGC 2 cut(s) 571, 612
TspDTI ATGAA 3 cut(s) 17, 429, 599
TspRI CASTG 1 cut(s) 232
XagI CCTNNNNNAGG 1 cut(s) 350
XapI RAATTY 2 cut(s) 5, 66
XbaI TCTAGA 1 cut(s) 191
XceI RCATGY 1 cut(s) 449
XhoI CTCGAG 1 cut(s) 121
XmaJI CCTAGG 1 cut(s) 346
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 2 cut(s) 192, 347
Zsp2I ATGCAT 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.