pycom12g23350

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001068
Physical Location & Seq
Forward (+)
23976877 .. 23980888
4012 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g23350.9

Sequence Viewer

Length: 1392 bp
ATGAAGATAAAGGTGCGATCTTTCGAATTCCATTCCATTTCTATTTTCTTTAAAACCTCACAGGGCGATGCCGACTCACCCCAATTTCTTTATCTTCATCAAATTTCTCTTCCATCAAGATTTCAACTTTCAGTCTGCCTCTGTGTAGTGATTACTGGAGATGTTGTAGTTGTAATGGCTTACATTCCTCCGCACAAGCGGCACTCGAAGGAACCAGAGAGGCCACTGCCGACTCCAGAGCTGCTTGCTCCTCAATTCAAGAAAAACTTCAAAGTCAAGCCATATAATAAGTCTAATTTGGAATGGACTGGAAAAATAGTTTATGCAGACCATTCTACATCGAGATGGCTGATCATTGGTTTGGATGAGGAGAACCAATTTCCGTCTTTTGTTAATCTTAAGCCCATTTGCTTGGAGTCCACTGAGCCGAAAGTTGGAGAAAACCGTCTAGCCTTAATCAATACTAGTCTAGATACTGAAGGCGGTGAGGTGAAATGGAATTTGCCAAGAAGCCCCGGGGAATCTCTAACGGAAAATGTGCTGGAAGACTTAGTTTCTTCTTTTAAACACGTGAGGAATGAAATGAAGTGTGCAAAACTTAAAGAAGTAAATCCTACTTTGATTGCCAGAGTGGGGAAAGTACTCTTTCGCAGGAGCCCGTCAGTTAACATGGAATCTATCACAAAAAATTTGTCTGCTGAAACCTTGAAACAGTTGAGGAAATCATTTCACACAAACATCCCTGTTTCATATAAGGAAAAGATTGTAAAGGAGGTTGTCCCGAAAGTTGGAGTTGATTTCGAAGAGGAGAAAGACCTATACCAAGTAAAGTTGTCTGATTCTACGAGGCCAGATTCAACTCTCTCCTGCAAGTGTCGTGTAATGAAAGAACATGGAATGCTACAACTCTACAAGATTGAACTGAATCAACTGCGTAACATGGTCGTGGACATTTCATGCACTACTAAGAATCTGGACCTGAGACTAATGCTATGCACGAAGAGACTCATAACAGATCTGACTGATGATGATATGCAAAGCATTAGGGATCTGATTAGTTTGGCGATTCTAGATCCAGATGTGAAGGGTGGGTTGAGATGGCCTCTGGGAAAGGAGTCTTCTGGAGATAAATACAAAGTTGTTGGGGTTTGGCATGTGATAGCTAATACGTATAAAAATTCATCGCTAAGACTGAAAGTGAGACATGCTGATCGATTTGATTTTAGAACCTTAACTGGAGAAGCTACTTGGGAGACAAGTCTGACGTTGAAAAAGGTCCTGTCAAAGTTACAGGAAGAGAATGTCGAAGCCAGCTCAATTTCGGAGATACTGAAGGAAGATATGCAGTTGATATGGGATAACTTCTTGAGCTGTGAACATTTTCTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

464

Amino Acids

52.96

Weight (kDa)

8.65

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 191, 199, 483
AclWI GGATC 2 cut(s) 1056, 1067
AcsI RAATTY 5 cut(s) 26, 102, 499, 688, 1177
AcuI CTGAAG 2 cut(s) 498, 1352
AcvI CACGTG 1 cut(s) 571
AfaI GTAC 1 cut(s) 642
AfiI CCNNNNNNNGG 3 cut(s) 434, 633, 788
AflII CTTAAG 1 cut(s) 398
AflIII ACRYGT 1 cut(s) 568
AgsI TTSAA 7 cut(s) 125, 259, 271, 709, 858, 920, 1270
AhlI ACTAGT 1 cut(s) 464
AluBI AGCT 5 cut(s) 241, 1163, 1244, 1314, 1371
AluI AGCT 5 cut(s) 241, 1163, 1244, 1314, 1371
Alw26I GTCTC 4 cut(s) 976, 997, 1195, 1247
AlwI GGATC 2 cut(s) 1056, 1067
Ama87I CYCGRG 1 cut(s) 515
AoxI GGCC 3 cut(s) 221, 848, 1100
ApeKI GCWGC 1 cut(s) 241
ApoI RAATTY 5 cut(s) 26, 102, 499, 688, 1177
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
Asp700I GAANNNNTTC 2 cut(s) 266, 1380
AspS9I GGNCC 2 cut(s) 976, 1276
AsuC2I CCSGG 2 cut(s) 516, 517
AsuHPI GGTGA 3 cut(s) 69, 497, 502
AsuII TTCGAA 2 cut(s) 24, 801
AvaI CYCGRG 1 cut(s) 515
AvaII GGWCC 2 cut(s) 976, 1276
BanII GRGCYC 1 cut(s) 659
BbrPI CACGTG 1 cut(s) 571
BbsI GAAGAC 2 cut(s) 552, 1110
BbvI GCAGC 1 cut(s) 228
BccI CCATC 3 cut(s) 121, 339, 1092
BclI TGATCA 1 cut(s) 351
BcnI CCSGG 2 cut(s) 516, 517
BcoDI GTCTC 4 cut(s) 976, 997, 1195, 1247
BcuI ACTAGT 1 cut(s) 464
BfaI CTAG 5 cut(s) 449, 465, 470, 1070, 1385
BfrI CTTAAG 1 cut(s) 398
BglII AGATCT 1 cut(s) 1015
BisI GCNGC 2 cut(s) 200, 242
BlsI GCNGC 2 cut(s) 201, 243
BmcAI AGTACT 1 cut(s) 642
Bme1390I CCNGG 2 cut(s) 516, 517
Bme18I GGWCC 2 cut(s) 976, 1276
BmeT110I CYCGRG 1 cut(s) 515
BmgT120I GGNCC 2 cut(s) 976, 1276
BmiI GGNNCC 2 cut(s) 213, 656
BmrFI CCNGG 2 cut(s) 516, 517
BmsI GCATC 1 cut(s) 58
BpiI GAAGAC 2 cut(s) 552, 1110
BplI GAGNNNNNCTC 2 cut(s) 1087, 1119
BpmI CTGGAG 4 cut(s) 177, 219, 1143, 1257
Bpu14I TTCGAA 2 cut(s) 24, 801
BpuEI CTTGAG 1 cut(s) 1387
BpuMI CCSGG 2 cut(s) 516, 517
Bsa29I ATCGAT 1 cut(s) 1213
BsaAI YACGTR 2 cut(s) 571, 1170
BsaJI CCNNGG 3 cut(s) 514, 515, 516
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 633, 788
Bse1I ACTGG 3 cut(s) 160, 313, 1240
BseCI ATCGAT 1 cut(s) 1213
BseDI CCNNGG 3 cut(s) 514, 515, 516
BseGI GGATG 2 cut(s) 370, 738
BseLI CCNNNNNNNGG 3 cut(s) 434, 633, 788
BseMII CTCAG 2 cut(s) 414, 971
BseNI ACTGG 3 cut(s) 160, 313, 1240
BseRI GAGGAG 3 cut(s) 240, 383, 821
BseXI GCAGC 1 cut(s) 228
BshFI GGCC 3 cut(s) 223, 850, 1102
BshVI ATCGAT 1 cut(s) 1213
BsiHKCI CYCGRG 1 cut(s) 515
BsiSI CCGG 1 cut(s) 516
BslFI GGGAC 1 cut(s) 764
BslI CCNNNNNNNGG 3 cut(s) 434, 633, 788
BsmAI GTCTC 4 cut(s) 976, 997, 1195, 1247
BsmFI GGGAC 1 cut(s) 764
BsmI GAATGC 1 cut(s) 903
BsnI GGCC 3 cut(s) 223, 850, 1102
BsoBI CYCGRG 1 cut(s) 515
Bsp119I TTCGAA 2 cut(s) 24, 801
Bsp1286I GDGCHC 1 cut(s) 659
Bsp143I GATC 6 cut(s) 17, 351, 1015, 1048, 1072, 1210
BspACI CCGC 3 cut(s) 191, 199, 483
BspANI GGCC 3 cut(s) 223, 850, 1102
BspCNI CTCAG 2 cut(s) 415, 972
BspDI ATCGAT 1 cut(s) 1213
BspLI GGNNCC 2 cut(s) 213, 656
BspPI GGATC 2 cut(s) 1056, 1067
BspT104I TTCGAA 2 cut(s) 24, 801
BspTI CTTAAG 1 cut(s) 398
BsrI ACTGG 3 cut(s) 160, 313, 1240
BssECI CCNNGG 3 cut(s) 514, 515, 516
BssMI GATC 6 cut(s) 17, 351, 1015, 1048, 1072, 1210
Bst4CI ACNGT 2 cut(s) 446, 714
Bst6I CTCTTC 4 cut(s) 114, 798, 995, 1290
BstAFI CTTAAG 1 cut(s) 398
BstBAI YACGTR 2 cut(s) 571, 1170
BstBI TTCGAA 2 cut(s) 24, 801
BstC8I GCNNGC 2 cut(s) 246, 1312
BstDEI CTNAG 5 cut(s) 423, 550, 966, 980, 1187
BstF5I GGATG 2 cut(s) 370, 738
BstKTI GATC 6 cut(s) 20, 354, 1018, 1051, 1075, 1213
BstMAI GTCTC 4 cut(s) 976, 997, 1195, 1247
BstMBI GATC 6 cut(s) 17, 351, 1015, 1048, 1072, 1210
BstMWI GCNNNNNNNGC 1 cut(s) 199
BstNSI RCATGY 2 cut(s) 1157, 1208
BstSCI CCNGG 2 cut(s) 514, 515
BstSNI TACGTA 1 cut(s) 1170
BstV1I GCAGC 1 cut(s) 228
BstV2I GAAGAC 2 cut(s) 552, 1110
BstX2I RGATCY 3 cut(s) 1015, 1048, 1072
BstXI CCANNNNNNTGG 1 cut(s) 412
BstYI RGATCY 3 cut(s) 1015, 1048, 1072
Bsu15I ATCGAT 1 cut(s) 1213
BsuRI GGCC 3 cut(s) 223, 850, 1102
BsuTUI ATCGAT 1 cut(s) 1213
BtgZI GCGATG 2 cut(s) 81, 1167
BtsCI GGATG 2 cut(s) 370, 738
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 2 cut(s) 224, 420
Cac8I GCNNGC 2 cut(s) 246, 1312
Cfr13I GGNCC 2 cut(s) 976, 1276
Cfr9I CCCGGG 1 cut(s) 515
ClaI ATCGAT 1 cut(s) 1213
Csp6I GTAC 1 cut(s) 641
CviAII CATG 7 cut(s) 670, 893, 940, 957, 1154, 1205, 1389
CviQI GTAC 1 cut(s) 641
DdeI CTNAG 5 cut(s) 423, 550, 966, 980, 1187
DpnI GATC 6 cut(s) 19, 353, 1017, 1050, 1074, 1212
DpnII GATC 6 cut(s) 17, 351, 1015, 1048, 1072, 1210
DraI TTTAAA 2 cut(s) 52, 565
Eam1104I CTCTTC 4 cut(s) 114, 798, 995, 1290
EarI CTCTTC 4 cut(s) 114, 798, 995, 1290
Eco105I TACGTA 1 cut(s) 1170
Eco24I GRGCYC 1 cut(s) 659
Eco47I GGWCC 2 cut(s) 976, 1276
Eco57I CTGAAG 2 cut(s) 498, 1352
Eco72I CACGTG 1 cut(s) 571
Eco88I CYCGRG 1 cut(s) 515
EcoO109I RGGNCCY 1 cut(s) 1276
EcoRI GAATTC 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 659
FaeI CATG 7 cut(s) 673, 896, 943, 960, 1157, 1208, 1392
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FaqI GGGAC 1 cut(s) 764
FatI CATG 7 cut(s) 669, 892, 939, 956, 1153, 1204, 1388
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 2 cut(s) 200, 242
FokI GGATG 2 cut(s) 377, 725
FriOI GRGCYC 1 cut(s) 659
Fsp4HI GCNGC 2 cut(s) 200, 242
FspBI CTAG 5 cut(s) 449, 465, 470, 1070, 1385
GluI GCNGC 2 cut(s) 200, 242
GsuI CTGGAG 4 cut(s) 177, 219, 1143, 1257
HaeIII GGCC 3 cut(s) 223, 850, 1102
HapII CCGG 1 cut(s) 516
Hin1II CATG 7 cut(s) 673, 896, 943, 960, 1157, 1208, 1392
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HpaI GTTAAC 1 cut(s) 667
HpaII CCGG 1 cut(s) 516
HphI GGTGA 3 cut(s) 69, 497, 502
Hpy166II GTNNAC 4 cut(s) 420, 667, 949, 1376
Hpy188I TCNGA 5 cut(s) 838, 1020, 1053, 1263, 1324
Hpy8I GTNNAC 4 cut(s) 420, 667, 949, 1376
HpyAV CCTTC 4 cut(s) 202, 473, 1078, 1327
HpyCH4III ACNGT 2 cut(s) 446, 714
HpyCH4IV ACGT 3 cut(s) 570, 1169, 1265
HpyCH4V TGCA 7 cut(s) 326, 593, 870, 960, 996, 1036, 1345
HpyF10VI GCNNNNNNNGC 1 cut(s) 199
HpyF3I CTNAG 5 cut(s) 423, 550, 966, 980, 1187
HpySE526I ACGT 3 cut(s) 570, 1169, 1265
Hsp92II CATG 7 cut(s) 673, 896, 943, 960, 1157, 1208, 1392
Ksp22I TGATCA 1 cut(s) 351
KspAI GTTAAC 1 cut(s) 667
Kzo9I GATC 6 cut(s) 17, 351, 1015, 1048, 1072, 1210
LmnI GCTCC 2 cut(s) 253, 654
Lsp1109I GCAGC 1 cut(s) 228
LweI GCATC 1 cut(s) 58
MaeI CTAG 5 cut(s) 449, 465, 470, 1070, 1385
MaeII ACGT 3 cut(s) 570, 1169, 1265
MaeIII GTNAC 2 cut(s) 935, 1287
MalI GATC 6 cut(s) 19, 353, 1017, 1050, 1074, 1212
MboI GATC 6 cut(s) 17, 351, 1015, 1048, 1072, 1210
MflI RGATCY 3 cut(s) 1015, 1048, 1072
MhlI GDGCHC 1 cut(s) 659
MlyI GAGTC 5 cut(s) 68, 226, 425, 999, 1124
MmeI TCCRAC 2 cut(s) 415, 769
MroXI GAANNNNTTC 2 cut(s) 266, 1380
MseI TTAA 8 cut(s) 51, 393, 399, 455, 564, 600, 666, 1232
MslI CAYNNNNRTG 2 cut(s) 343, 944
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 1 cut(s) 516
MspR9I CCNGG 2 cut(s) 516, 517
Mva1269I GAATGC 1 cut(s) 903
MwoI GCNNNNNNNGC 1 cut(s) 199
NciI CCSGG 2 cut(s) 516, 517
NdeII GATC 6 cut(s) 17, 351, 1015, 1048, 1072, 1210
NlaIII CATG 7 cut(s) 673, 896, 943, 960, 1157, 1208, 1392
NlaIV GGNNCC 2 cut(s) 213, 656
NspI RCATGY 2 cut(s) 1157, 1208
NspV TTCGAA 2 cut(s) 24, 801
PctI GAATGC 1 cut(s) 903
PdmI GAANNNNTTC 2 cut(s) 266, 1380
PfeI GAWTC 7 cut(s) 521, 674, 839, 854, 925, 970, 1066
PkrI GCNGC 2 cut(s) 201, 243
PleI GAGTC 5 cut(s) 68, 226, 424, 999, 1123
PmaCI CACGTG 1 cut(s) 571
PmlI CACGTG 1 cut(s) 571
PpsI GAGTC 5 cut(s) 68, 226, 424, 999, 1123
Ppu21I YACGTR 2 cut(s) 571, 1170
PpuMI RGGWCCY 1 cut(s) 1276
Psp5II RGGWCCY 1 cut(s) 1276
PspCI CACGTG 1 cut(s) 571
PspN4I GGNNCC 2 cut(s) 213, 656
PspPI GGNCC 2 cut(s) 976, 1276
PspPPI RGGWCCY 1 cut(s) 1276
PsuI RGATCY 3 cut(s) 1015, 1048, 1072
RsaI GTAC 1 cut(s) 642
RsaNI GTAC 1 cut(s) 641
RseI CAYNNNNRTG 2 cut(s) 343, 944
SaqAI TTAA 8 cut(s) 51, 393, 399, 455, 564, 600, 666, 1232
SatI GCNGC 2 cut(s) 200, 242
Sau3AI GATC 6 cut(s) 17, 351, 1015, 1048, 1072, 1210
Sau96I GGNCC 2 cut(s) 976, 1276
ScaI AGTACT 1 cut(s) 642
SchI GAGTC 5 cut(s) 68, 226, 425, 999, 1124
ScrFI CCNGG 2 cut(s) 516, 517
SduI GDGCHC 1 cut(s) 659
SfaNI GCATC 1 cut(s) 58
SfuI TTCGAA 2 cut(s) 24, 801
SinI GGWCC 2 cut(s) 976, 1276
SmaI CCCGGG 1 cut(s) 517
SmiMI CAYNNNNRTG 2 cut(s) 343, 944
SmlI CTYRAG 2 cut(s) 398, 1366
SmoI CTYRAG 2 cut(s) 398, 1366
SnaBI TACGTA 1 cut(s) 1170
SpeI ACTAGT 1 cut(s) 464
SsiI CCGC 3 cut(s) 191, 199, 483
SspMI CTAG 5 cut(s) 449, 465, 470, 1070, 1385
StyD4I CCNGG 2 cut(s) 514, 515
TaaI ACNGT 2 cut(s) 446, 714
TaiI ACGT 3 cut(s) 573, 1172, 1268
TaqI TCGA 6 cut(s) 24, 206, 341, 801, 1213, 1305
TatI WGTACW 1 cut(s) 640
TauI GCSGC 1 cut(s) 202
TfiI GAWTC 7 cut(s) 521, 674, 839, 854, 925, 970, 1066
Tru1I TTAA 8 cut(s) 51, 393, 399, 455, 564, 600, 666, 1232
Tru9I TTAA 8 cut(s) 51, 393, 399, 455, 564, 600, 666, 1232
TscAI CASTG 2 cut(s) 231, 427
TseI GCWGC 1 cut(s) 241
TspDTI ATGAA 8 cut(s) 17, 86, 594, 599, 738, 899, 945, 1170
TspGWI ACGGA 2 cut(s) 372, 545
TspMI CCCGGG 1 cut(s) 515
TspRI CASTG 2 cut(s) 231, 427
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 2 cut(s) 976, 1276
XapI RAATTY 5 cut(s) 26, 102, 499, 688, 1177
XbaI TCTAGA 2 cut(s) 469, 1069
XceI RCATGY 2 cut(s) 1157, 1208
XmaI CCCGGG 1 cut(s) 515
XmnI GAANNNNTTC 2 cut(s) 266, 1380
XspI CTAG 5 cut(s) 449, 465, 470, 1070, 1385
ZrmI AGTACT 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.