MD12G1254900.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001068
Physical Location & Seq
Forward (+)
32318769 .. 32322064
3296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1254900.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGCTTACATTCCTCCGCACAAGCGGCACTCGAAGGAACCGGAGAGGCCACTGCCGACTCCAGAGCTGCTTGCGCCTCAATTCAAGAAAAACTTCAAAGACAAGCCATATAATAAGTCTAATGTGGAATGGACTGGAAAAATAGTTTATGCAGACCATTCTACATCAAGATGGCTGACGATTGGTTTGGATGAGGAGAACCAATTTCCGCCTTCTGTTAATCTTAAGCCCATTTGCTTGGAGTCCACTGAGCCGAAAGTTGGAGAAAACCGTCTAGCTTTAATCAATACTAGTCTAGATAATGAAGGCGGTGAGGTGAAATGGAATTTGCCAAGAAACCCCGGGGAATCTCTAACAGAAAATGTGCTGGAAGACTTAGTTTCTTCTTTTAAACATGTGAGGAATGAAATGAAGTGCGCAAAGCTCAAAGGAGTAAATCCTACTTTGATTGCCAGAGTGGGGAAAGTACTGTTTCGCAGGAGCCCGTCGGTTAACATGGAATCTATCACAAAAAATTTGTCTACTGAAACCTTGAAACAGTTGAGGAAATCATTTCACACAAACATCCCTGTTTCATATAAGGAAAAGATTGTAAAGGAGGTTGTCCCGAAAGTTGGAGTTGATTTCGAAGAGGAGAAAGACGTATACCAAGTAAAGTTGTCTGATTCTACGAGGCCAGATTCAACTCTCTCCTGCAAGTGTCGTGTAATGAAAGAACATGGAATGCTACAACTCTACAAGATTGAACTGAATCAACTGCGTAACATGGTCGTGGACATTTCATGCACTACTAAGAATCTGGACCTGAGACTAATGCTATGCACAAAGAGACTCGTAACAGATCTGACTGATGATGATATGCAAAGCATTAGGGATCTGATTAGTTTGGCGATTCTAGATCCAGATGTGAAGGGTGGGTTGAGATGGCCTCTGGGAAAGGAGTCTTCTGGAGATAAATACAAAGTTGTTGGGGTTTGGCATGTGATAGCTAATACGTATAAAAATTCATCGCTGAGACTGAAAGTGAGACATGCTGATCGATTTGATTTTAGAACCTTAACTGGGGAAGCTACTTGGGAAACAAGTCTGATGTTGAAAAAGGTCCTGTCAAAGTTACAGGAGAATGTCGAAGCCAGCTCAATTTCGGAGATACTGAAGGAAGATATGCAGTTGATATGGGATAACTTCTTGAGCTGTGAACATTATCTAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

405

Amino Acids

46.27

Weight (kDa)

8.74

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7903 PF25475 44 - 396 2.6e-157 Domain of unknown function (DUF7903)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 418
AccI GTMKAC 2 cut(s) 521, 645
AciI CCGC 4 cut(s) 17, 25, 209, 309
AclWI GGATC 2 cut(s) 882, 893
AcsI RAATTY 3 cut(s) 325, 514, 1003
AcuI CTGAAG 1 cut(s) 1175
AfaI GTAC 1 cut(s) 468
AfiI CCNNNNNNNGG 4 cut(s) 260, 459, 614, 1062
AflII CTTAAG 1 cut(s) 224
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 6 cut(s) 85, 97, 535, 684, 746, 1096
AhlI ACTAGT 1 cut(s) 290
AluBI AGCT 7 cut(s) 67, 278, 424, 989, 1070, 1137, 1194
AluI AGCT 7 cut(s) 67, 278, 424, 989, 1070, 1137, 1194
Alw26I GTCTC 4 cut(s) 802, 823, 1009, 1021
AlwI GGATC 2 cut(s) 882, 893
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 3 cut(s) 47, 674, 926
ApeKI GCWGC 1 cut(s) 67
ApoI RAATTY 3 cut(s) 325, 514, 1003
ArsI GACNNNNNNTTYG 4 cut(s) 169, 201, 503, 535
Asp700I GAANNNNTTC 1 cut(s) 92
AspLEI GCGC 2 cut(s) 76, 419
AspS9I GGNCC 2 cut(s) 802, 1102
AsuC2I CCSGG 2 cut(s) 342, 343
AsuHPI GGTGA 2 cut(s) 323, 328
AsuII TTCGAA 1 cut(s) 627
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 2 cut(s) 802, 1102
BanII GRGCYC 1 cut(s) 485
BbsI GAAGAC 2 cut(s) 378, 936
BbvI GCAGC 1 cut(s) 54
BccI CCATC 2 cut(s) 165, 918
BcnI CCSGG 2 cut(s) 342, 343
BcoDI GTCTC 4 cut(s) 802, 823, 1009, 1021
BcuI ACTAGT 1 cut(s) 290
BfaI CTAG 4 cut(s) 275, 291, 296, 896
BfrI CTTAAG 1 cut(s) 224
BglII AGATCT 1 cut(s) 841
BisI GCNGC 2 cut(s) 26, 68
BlsI GCNGC 2 cut(s) 27, 69
BmcAI AGTACT 1 cut(s) 468
Bme1390I CCNGG 2 cut(s) 342, 343
Bme18I GGWCC 2 cut(s) 802, 1102
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 2 cut(s) 802, 1102
BmiI GGNNCC 2 cut(s) 39, 482
BmrFI CCNGG 2 cut(s) 342, 343
BmrI ACTGGG 1 cut(s) 1071
BmuI ACTGGG 1 cut(s) 1071
BpiI GAAGAC 2 cut(s) 378, 936
BplI GAGNNNNNCTC 2 cut(s) 913, 945
BpmI CTGGAG 2 cut(s) 45, 969
Bpu14I TTCGAA 1 cut(s) 627
BpuEI CTTGAG 1 cut(s) 1210
BpuMI CCSGG 2 cut(s) 342, 343
Bsa29I ATCGAT 1 cut(s) 1039
BsaAI YACGTR 1 cut(s) 996
BsaJI CCNNGG 3 cut(s) 340, 341, 342
BsaWI WCCGGW 1 cut(s) 40
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 459, 614, 1062
Bse1I ACTGG 2 cut(s) 139, 1066
BseCI ATCGAT 1 cut(s) 1039
BseDI CCNNGG 3 cut(s) 340, 341, 342
BseGI GGATG 2 cut(s) 196, 564
BseLI CCNNNNNNNGG 4 cut(s) 260, 459, 614, 1062
BseMII CTCAG 3 cut(s) 240, 797, 1004
BseNI ACTGG 2 cut(s) 139, 1066
BseRI GAGGAG 2 cut(s) 209, 647
BseXI GCAGC 1 cut(s) 54
BshFI GGCC 3 cut(s) 49, 676, 928
BshVI ATCGAT 1 cut(s) 1039
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 2 cut(s) 41, 342
BslFI GGGAC 1 cut(s) 590
BslI CCNNNNNNNGG 4 cut(s) 260, 459, 614, 1062
BsmAI GTCTC 4 cut(s) 802, 823, 1009, 1021
BsmFI GGGAC 1 cut(s) 590
BsmI GAATGC 1 cut(s) 729
BsnI GGCC 3 cut(s) 49, 676, 928
BsoBI CYCGRG 1 cut(s) 341
Bsp119I TTCGAA 1 cut(s) 627
Bsp1286I GDGCHC 1 cut(s) 485
Bsp143I GATC 4 cut(s) 841, 874, 898, 1036
BspACI CCGC 4 cut(s) 17, 25, 209, 309
BspANI GGCC 3 cut(s) 49, 676, 928
BspCNI CTCAG 3 cut(s) 241, 798, 1005
BspDI ATCGAT 1 cut(s) 1039
BspLI GGNNCC 2 cut(s) 39, 482
BspPI GGATC 2 cut(s) 882, 893
BspT104I TTCGAA 1 cut(s) 627
BspTI CTTAAG 1 cut(s) 224
BsrI ACTGG 2 cut(s) 139, 1066
BssECI CCNNGG 3 cut(s) 340, 341, 342
BssMI GATC 4 cut(s) 841, 874, 898, 1036
BssNAI GTATAC 1 cut(s) 646
Bst1107I GTATAC 1 cut(s) 646
Bst4CI ACNGT 3 cut(s) 272, 471, 540
Bst6I CTCTTC 1 cut(s) 624
BstAFI CTTAAG 1 cut(s) 224
BstBAI YACGTR 1 cut(s) 996
BstBI TTCGAA 1 cut(s) 627
BstC8I GCNNGC 2 cut(s) 72, 1135
BstDEI CTNAG 5 cut(s) 249, 376, 792, 806, 1013
BstF5I GGATG 2 cut(s) 196, 564
BstHHI GCGC 2 cut(s) 76, 419
BstKTI GATC 4 cut(s) 844, 877, 901, 1039
BstMAI GTCTC 4 cut(s) 802, 823, 1009, 1021
BstMBI GATC 4 cut(s) 841, 874, 898, 1036
BstMWI GCNNNNNNNGC 2 cut(s) 25, 73
BstNSI RCATGY 3 cut(s) 398, 983, 1034
BstSCI CCNGG 2 cut(s) 340, 341
BstSNI TACGTA 1 cut(s) 996
BstV1I GCAGC 1 cut(s) 54
BstV2I GAAGAC 2 cut(s) 378, 936
BstX2I RGATCY 3 cut(s) 841, 874, 898
BstXI CCANNNNNNTGG 1 cut(s) 238
BstYI RGATCY 3 cut(s) 841, 874, 898
BstZ17I GTATAC 1 cut(s) 646
Bsu15I ATCGAT 1 cut(s) 1039
BsuRI GGCC 3 cut(s) 49, 676, 928
BsuTUI ATCGAT 1 cut(s) 1039
BtgZI GCGATG 1 cut(s) 993
BtsCI GGATG 2 cut(s) 196, 564
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 2 cut(s) 50, 246
Cac8I GCNNGC 2 cut(s) 72, 1135
CfoI GCGC 2 cut(s) 76, 419
Cfr13I GGNCC 2 cut(s) 802, 1102
Cfr9I CCCGGG 1 cut(s) 341
ClaI ATCGAT 1 cut(s) 1039
Csp6I GTAC 1 cut(s) 467
CviAII CATG 8 cut(s) 395, 496, 719, 766, 783, 980, 1031, 1212
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 5 cut(s) 249, 376, 792, 806, 1013
DpnI GATC 4 cut(s) 843, 876, 900, 1038
DpnII GATC 4 cut(s) 841, 874, 898, 1036
DraI TTTAAA 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 624
EarI CTCTTC 1 cut(s) 624
EciI GGCGGA 1 cut(s) 198
Eco105I TACGTA 1 cut(s) 996
Eco24I GRGCYC 1 cut(s) 485
Eco47I GGWCC 2 cut(s) 802, 1102
Eco57I CTGAAG 1 cut(s) 1175
Eco88I CYCGRG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 1102
EcoT38I GRGCYC 1 cut(s) 485
FaeI CATG 8 cut(s) 398, 499, 722, 769, 786, 983, 1034, 1215
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FaqI GGGAC 1 cut(s) 590
FatI CATG 8 cut(s) 394, 495, 718, 765, 782, 979, 1030, 1211
FblI GTMKAC 2 cut(s) 521, 645
Fnu4HI GCNGC 2 cut(s) 26, 68
FokI GGATG 2 cut(s) 203, 551
FriOI GRGCYC 1 cut(s) 485
Fsp4HI GCNGC 2 cut(s) 26, 68
FspBI CTAG 4 cut(s) 275, 291, 296, 896
FspI TGCGCA 1 cut(s) 418
GlaI GCGC 2 cut(s) 75, 418
GluI GCNGC 2 cut(s) 26, 68
GsuI CTGGAG 2 cut(s) 45, 969
HaeIII GGCC 3 cut(s) 49, 676, 928
HapII CCGG 2 cut(s) 41, 342
HhaI GCGC 2 cut(s) 76, 419
Hin1II CATG 8 cut(s) 398, 499, 722, 769, 786, 983, 1034, 1215
Hin6I GCGC 2 cut(s) 74, 417
HinP1I GCGC 2 cut(s) 74, 417
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HpaI GTTAAC 1 cut(s) 493
HpaII CCGG 2 cut(s) 41, 342
HphI GGTGA 2 cut(s) 323, 328
Hpy166II GTNNAC 6 cut(s) 246, 493, 522, 646, 775, 1199
Hpy188I TCNGA 5 cut(s) 664, 846, 879, 1089, 1147
Hpy8I GTNNAC 6 cut(s) 246, 493, 522, 646, 775, 1199
Hpy99I CGWCG 1 cut(s) 490
HpyAV CCTTC 5 cut(s) 28, 222, 299, 904, 1150
HpyCH4III ACNGT 3 cut(s) 272, 471, 540
HpyCH4IV ACGT 2 cut(s) 642, 995
HpyCH4V TGCA 6 cut(s) 152, 696, 786, 822, 862, 1168
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 73
HpyF3I CTNAG 5 cut(s) 249, 376, 792, 806, 1013
HpySE526I ACGT 2 cut(s) 642, 995
Hsp92II CATG 8 cut(s) 398, 499, 722, 769, 786, 983, 1034, 1215
HspAI GCGC 2 cut(s) 74, 417
KspAI GTTAAC 1 cut(s) 493
Kzo9I GATC 4 cut(s) 841, 874, 898, 1036
LmnI GCTCC 1 cut(s) 480
Lsp1109I GCAGC 1 cut(s) 54
MaeI CTAG 4 cut(s) 275, 291, 296, 896
MaeII ACGT 2 cut(s) 642, 995
MaeIII GTNAC 3 cut(s) 761, 835, 1113
MalI GATC 4 cut(s) 843, 876, 900, 1038
MboI GATC 4 cut(s) 841, 874, 898, 1036
MboII GAAGA 5 cut(s) 375, 383, 641, 936, 1172
MflI RGATCY 3 cut(s) 841, 874, 898
MhlI GDGCHC 1 cut(s) 485
MluCI AATT 6 cut(s) 80, 203, 325, 514, 1003, 1140
MlyI GAGTC 4 cut(s) 52, 251, 825, 950
MmeI TCCRAC 2 cut(s) 241, 595
MroXI GAANNNNTTC 1 cut(s) 92
MseI TTAA 6 cut(s) 219, 225, 281, 390, 492, 1058
MslI CAYNNNNRTG 2 cut(s) 169, 770
MspCI CTTAAG 1 cut(s) 224
MspI CCGG 2 cut(s) 41, 342
MspR9I CCNGG 2 cut(s) 342, 343
Mva1269I GAATGC 1 cut(s) 729
MwoI GCNNNNNNNGC 2 cut(s) 25, 73
NciI CCSGG 2 cut(s) 342, 343
NdeII GATC 4 cut(s) 841, 874, 898, 1036
NlaIII CATG 8 cut(s) 398, 499, 722, 769, 786, 983, 1034, 1215
NlaIV GGNNCC 2 cut(s) 39, 482
NsbI TGCGCA 1 cut(s) 418
NspI RCATGY 3 cut(s) 398, 983, 1034
NspV TTCGAA 1 cut(s) 627
PciI ACATGT 1 cut(s) 394
PctI GAATGC 1 cut(s) 729
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 7 cut(s) 347, 500, 665, 680, 751, 796, 892
PkrI GCNGC 2 cut(s) 27, 69
PleI GAGTC 4 cut(s) 52, 250, 825, 949
PpsI GAGTC 4 cut(s) 52, 250, 825, 949
Ppu21I YACGTR 1 cut(s) 996
PpuMI RGGWCCY 1 cut(s) 1102
PscI ACATGT 1 cut(s) 394
Psp5II RGGWCCY 1 cut(s) 1102
PspN4I GGNNCC 2 cut(s) 39, 482
PspPI GGNCC 2 cut(s) 802, 1102
PspPPI RGGWCCY 1 cut(s) 1102
PsuI RGATCY 3 cut(s) 841, 874, 898
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
RseI CAYNNNNRTG 2 cut(s) 169, 770
SaqAI TTAA 6 cut(s) 219, 225, 281, 390, 492, 1058
SatI GCNGC 2 cut(s) 26, 68
Sau3AI GATC 4 cut(s) 841, 874, 898, 1036
Sau96I GGNCC 2 cut(s) 802, 1102
ScaI AGTACT 1 cut(s) 468
SchI GAGTC 4 cut(s) 52, 251, 825, 950
ScrFI CCNGG 2 cut(s) 342, 343
SduI GDGCHC 1 cut(s) 485
SfuI TTCGAA 1 cut(s) 627
SinI GGWCC 2 cut(s) 802, 1102
SmaI CCCGGG 1 cut(s) 343
SmiMI CAYNNNNRTG 2 cut(s) 169, 770
SmlI CTYRAG 2 cut(s) 224, 1189
SmoI CTYRAG 2 cut(s) 224, 1189
SnaBI TACGTA 1 cut(s) 996
SpeI ACTAGT 1 cut(s) 290
Sse9I AATT 6 cut(s) 80, 203, 325, 514, 1003, 1140
SsiI CCGC 4 cut(s) 17, 25, 209, 309
SspMI CTAG 4 cut(s) 275, 291, 296, 896
StyD4I CCNGG 2 cut(s) 340, 341
TaaI ACNGT 3 cut(s) 272, 471, 540
TaiI ACGT 2 cut(s) 645, 998
TaqI TCGA 4 cut(s) 32, 627, 1039, 1128
TasI AATT 6 cut(s) 80, 203, 325, 514, 1003, 1140
TatI WGTACW 1 cut(s) 466
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 7 cut(s) 347, 500, 665, 680, 751, 796, 892
Tru1I TTAA 6 cut(s) 219, 225, 281, 390, 492, 1058
Tru9I TTAA 6 cut(s) 219, 225, 281, 390, 492, 1058
TscAI CASTG 2 cut(s) 57, 253
TseI GCWGC 1 cut(s) 67
TspDTI ATGAA 7 cut(s) 318, 420, 425, 564, 725, 771, 996
TspMI CCCGGG 1 cut(s) 341
TspRI CASTG 2 cut(s) 57, 253
Vha464I CTTAAG 1 cut(s) 224
VpaK11BI GGWCC 2 cut(s) 802, 1102
XapI RAATTY 3 cut(s) 325, 514, 1003
XbaI TCTAGA 2 cut(s) 295, 895
XceI RCATGY 3 cut(s) 398, 983, 1034
XmaI CCCGGG 1 cut(s) 341
XmiI GTMKAC 2 cut(s) 521, 645
XmnI GAANNNNTTC 1 cut(s) 92
XspI CTAG 4 cut(s) 275, 291, 296, 896
ZrmI AGTACT 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.