MD12G1254800.v1.1

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001068
Physical Location & Seq
Forward (+)
32314428 .. 32317579
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1254800.v1.1.491

Sequence Viewer

Length: 1740 bp
ATGGCGTCTAACTCTTCCAGCTCTTCCAACGTTTCCAACTTTCTTACGATTAAACTTGACCCTACCAATTACCCTCTCTGGCAAGCCCAGATGCTTCCCTTGCTTCGTAGCAGGAATCTCGTCTCCTTTGTTGATGGCACTAGCAAATGTCCTCCTGCCTTTCTCAAAGATGATGAGGGCAAACTTACTCTGAATCCGGAGTTTAAAGCATGGGTTCAACAAGATATTATGGTCATGTCCTGGATCAAGAGCTCTGTTCATCCCACTGTGTTGGCTGCCCTAATCGGGAAAACCAGTTCTCATTCTGCATGGACCTGTCTGCGCGAACGCTATGATTTGCAGCCCACTGCTCAGGGTCGTGATGAAGCCATATCTCACAGTGCTTCAGAGGCCCTTCTGCTCACTGATGAGCTGAAACAAAGGATGCATACTGTCTTCCCTTCTGATACTAGACCCACTGCTCCCGCTGCTGCTCGTGGCGGTCGTCCTGGTACCTTCCGTGGTCGTGGCAAAGGCTATAGAGGCATAAGTGCTAGTTCTGGAATGGCTCACATTCCACCACACAAGTGGCACTCGAAGGAATCGGAGAGGCCATTGCTGACTTCAGAGCTGCGTGCTCAACGATTCAAGCCATATAACAAGTATAATGTGGATCCGACTGGAAGAATAGCTCATGCAGACCATTCTACATATAGATGGTGCGCCGTTGCTTTGGGTGAGGAGAACCAATTTCCGCCTTCTGTTAATCTTAAGCCCATTTGCTTGGATTCCACTGAGCCGAAAGTTGAAGAAAATCGTCTAGCTTTAATCAACACTAGTCTAGATGATGAAGGCGGTGAGTTGAAATGGAATTCGCCAAGGAGCCCTGGGGTATCTATAGCAGAAAATGTGCTGGAAGACTTGGTTTCTTCTTTTAAACACATGAGGAATGAAATGAAGTGCGCGCAGCTCAAAGAAGGAAGGCCTACTTTGCTTGCTAGAGTGGGGAAAGTACTTTTTCTTAGGAGCCGTTTAGTTAACATGGAAGCCATCAGAACAAATTTGTGTACTGAAATCTTGAAACAATGGAGACGATTATTGTACACAAACATTCCAGTTTCATATAAGGAAAGGATTGTGAACGAAGTTGTCCCGAAAATTGGAGTTGATTTCGAAGGGGAGAAAGATTCATACCAAGTAAAGTTGTTTGATTCCACCAGATCAGATGCAACTCTCTCCTGCAAATGCCGTGTAACGAAAGAACATGGAAAGCTACAACTCTACAAGATTGAACTGAATCAACTGCGTAACATGGTCGTGGACATTTCATGCACTACCAAAGATCTGGACCTGAGACTGATGCTATGCACGAAGAGACTCGTCACCGATCTGACTGACGATGAGATGCAAAGCATTCGGAATCTGATTGATTCGGCGATTCTAGATCCAGATGTGAACGGCGGGTTGAGATGGCCTTCGGGAAAGGATTCTTCCGGAAAATACAAAGTTGATGGGGTTTGGCACTTAATAGCTAATACGTATAAAAATTCATCGTTCAGACTGAAAGTCAGACATGCGGATCGATTTGATTTTAGAACCCTAACTGGGGGAACTACGTGGGAGACAATTCTGATGTTGAAAAGGGTCGTGTCAAAGTTACAGGAAGAGAATGCCGAAGCCAGCTCGGTTTCTGAGATACTGAATGAAGACATGCAGCTGATATGGGACAACTTCTTGAGCTGTGAAGGATTCCTGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

580

Amino Acids

65.39

Weight (kDa)

8.78

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7903 PF25475 220 - 571 4.9e-146 Domain of unknown function (DUF7903)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 491
AccB1I GGYRCC 1 cut(s) 491
AccII CGCG 2 cut(s) 324, 944
AccIII TCCGGA 2 cut(s) 196, 1472
AciI CCGC 6 cut(s) 465, 480, 734, 834, 1440, 1556
AclI AACGTT 1 cut(s) 30
AclWI GGATC 5 cut(s) 251, 647, 660, 1418, 1566
AcsI RAATTY 3 cut(s) 850, 1039, 1525
AcuI CTGAAG 2 cut(s) 369, 588
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 493, 993, 1048, 1082
AfiI CCNNNNNNNGG 4 cut(s) 285, 1139, 1584, 1585
AflII CTTAAG 1 cut(s) 749
AgsI TTSAA 7 cut(s) 218, 628, 788, 844, 1060, 1271, 1618
AhdI GACNNNNNGTC 1 cut(s) 1544
AhlI ACTAGT 1 cut(s) 815
AjiI CACGTC 1 cut(s) 1737
AjnI CCWGG 3 cut(s) 239, 487, 865
AleI CACNNNNGTG 1 cut(s) 565
AloI GAACNNNNNNTCC 2 cut(s) 280, 312
Alw21I GWGCWC 2 cut(s) 254, 619
Alw26I GTCTC 5 cut(s) 127, 1063, 1327, 1348, 1595
AlwI GGATC 5 cut(s) 251, 647, 660, 1418, 1566
Aor13HI TCCGGA 2 cut(s) 196, 1472
AoxI GGCC 4 cut(s) 390, 590, 962, 1451
ApeKI GCWGC 7 cut(s) 275, 340, 467, 470, 610, 946, 1693
ApoI RAATTY 3 cut(s) 850, 1039, 1525
Asp700I GAANNNNTTC 1 cut(s) 1465
Asp718I GGTACC 1 cut(s) 491
AspLEI GCGC 4 cut(s) 324, 704, 944, 946
AspS9I GGNCC 3 cut(s) 312, 391, 1327
AsuHPI GGTGA 3 cut(s) 728, 848, 1354
AsuII TTCGAA 1 cut(s) 1152
AvaII GGWCC 2 cut(s) 312, 1327
BamHI GGATCC 1 cut(s) 652
BanI GGYRCC 1 cut(s) 491
BanII GRGCYC 2 cut(s) 254, 866
BauI CACGAG 1 cut(s) 474
BbsI GAAGAC 3 cut(s) 427, 903, 1692
Bbv12I GWGCWC 2 cut(s) 254, 619
BbvI GCAGC 7 cut(s) 262, 352, 454, 457, 597, 958, 1705
BccI CCATC 5 cut(s) 128, 690, 1037, 1443, 1484
BceAI ACGGC 4 cut(s) 689, 993, 1212, 1453
BcgI CGANNNNNNTGC 4 cut(s) 100, 134, 1376, 1410
BciT130I CCWGG 3 cut(s) 241, 489, 867
BcoDI GTCTC 5 cut(s) 127, 1063, 1327, 1348, 1595
BcuI ACTAGT 1 cut(s) 815
BfaI CTAG 8 cut(s) 141, 450, 534, 800, 816, 821, 978, 1421
BfmI CTRYAG 2 cut(s) 517, 876
BfrI CTTAAG 1 cut(s) 749
BglII AGATCT 1 cut(s) 1321
BisI GCNGC 7 cut(s) 276, 341, 468, 471, 611, 947, 1694
BlsI GCNGC 7 cut(s) 277, 342, 469, 472, 612, 948, 1695
BmcAI AGTACT 1 cut(s) 993
Bme1390I CCNGG 3 cut(s) 241, 489, 867
Bme18I GGWCC 2 cut(s) 312, 1327
BmeRI GACNNNNNGTC 1 cut(s) 1544
BmgBI CACGTC 1 cut(s) 1737
BmgT120I GGNCC 3 cut(s) 312, 391, 1327
BmiI GGNNCC 4 cut(s) 493, 654, 863, 1007
BmrFI CCNGG 3 cut(s) 241, 489, 867
BmrI ACTGGG 1 cut(s) 1593
BmsI GCATC 5 cut(s) 81, 414, 1195, 1329, 1374
BmuI ACTGGG 1 cut(s) 1593
BpiI GAAGAC 3 cut(s) 427, 903, 1692
Bpu10I CCTNAGC 1 cut(s) 351
Bpu14I TTCGAA 1 cut(s) 1152
BpuEI CTTGAG 1 cut(s) 1735
Bsa29I ATCGAT 1 cut(s) 1561
BsaAI YACGTR 2 cut(s) 1518, 1596
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 499, 857, 865, 866
BsaWI WCCGGW 2 cut(s) 196, 1472
Bsc4I CCNNNNNNNGG 4 cut(s) 285, 1139, 1584, 1585
Bse1I ACTGG 4 cut(s) 294, 664, 1094, 1588
Bse3DI GCAATG 1 cut(s) 593
BseAI TCCGGA 2 cut(s) 196, 1472
BseBI CCWGG 3 cut(s) 241, 489, 867
BseCI ATCGAT 1 cut(s) 1561
BseDI CCNNGG 4 cut(s) 499, 857, 865, 866
BseGI GGATG 2 cut(s) 259, 429
BseLI CCNNNNNNNGG 4 cut(s) 285, 1139, 1584, 1585
BseMI GCAATG 1 cut(s) 593
BseMII CTCAG 4 cut(s) 365, 765, 1322, 1662
BseNI ACTGG 4 cut(s) 294, 664, 1094, 1588
BsePI GCGCGC 1 cut(s) 942
BseRI GAGGAG 1 cut(s) 734
BseXI GCAGC 7 cut(s) 262, 352, 454, 457, 597, 958, 1705
Bsh1236I CGCG 2 cut(s) 324, 944
Bsh1285I CGRYCG 1 cut(s) 484
BshFI GGCC 4 cut(s) 392, 592, 964, 1453
BshNI GGYRCC 1 cut(s) 491
BshVI ATCGAT 1 cut(s) 1561
BsiEI CGRYCG 1 cut(s) 484
BsiHKAI GWGCWC 2 cut(s) 254, 619
BsiSI CCGG 2 cut(s) 197, 1473
BslFI GGGAC 2 cut(s) 1115, 1718
BslI CCNNNNNNNGG 4 cut(s) 285, 1139, 1584, 1585
BsmAI GTCTC 5 cut(s) 127, 1063, 1327, 1348, 1595
BsmBI CGTCTC 2 cut(s) 127, 1063
BsmFI GGGAC 2 cut(s) 1115, 1718
BsmI GAATGC 2 cut(s) 1392, 1654
BsnI GGCC 4 cut(s) 392, 592, 964, 1453
Bsp119I TTCGAA 1 cut(s) 1152
Bsp1286I GDGCHC 3 cut(s) 254, 619, 866
Bsp13I TCCGGA 2 cut(s) 196, 1472
Bsp1407I TGTACA 1 cut(s) 1080
Bsp143I GATC 7 cut(s) 243, 652, 1199, 1321, 1366, 1423, 1558
BspACI CCGC 6 cut(s) 465, 480, 734, 834, 1440, 1556
BspANI GGCC 4 cut(s) 392, 592, 964, 1453
BspCNI CTCAG 4 cut(s) 364, 766, 1323, 1663
BspDI ATCGAT 1 cut(s) 1561
BspEI TCCGGA 2 cut(s) 196, 1472
BspFNI CGCG 2 cut(s) 324, 944
BspLI GGNNCC 4 cut(s) 493, 654, 863, 1007
BspPI GGATC 5 cut(s) 251, 647, 660, 1418, 1566
BspQI GCTCTTC 1 cut(s) 28
BspT104I TTCGAA 1 cut(s) 1152
BspT107I GGYRCC 1 cut(s) 491
BspTI CTTAAG 1 cut(s) 749
BsrDI GCAATG 1 cut(s) 593
BsrGI TGTACA 1 cut(s) 1080
BsrI ACTGG 4 cut(s) 294, 664, 1094, 1588
BssECI CCNNGG 4 cut(s) 499, 857, 865, 866
BssHII GCGCGC 1 cut(s) 942
BssMI GATC 7 cut(s) 243, 652, 1199, 1321, 1366, 1423, 1558
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 857
Bst2BI CACGAG 1 cut(s) 474
Bst2UI CCWGG 3 cut(s) 241, 489, 867
Bst4CI ACNGT 3 cut(s) 268, 380, 433
Bst6I CTCTTC 4 cut(s) 19, 28, 1346, 1638
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 749
BstAUI TGTACA 1 cut(s) 1080
BstBAI YACGTR 2 cut(s) 1518, 1596
BstBI TTCGAA 1 cut(s) 1152
BstC8I GCNNGC 5 cut(s) 84, 615, 944, 975, 1660
BstDEI CTNAG 5 cut(s) 351, 774, 1001, 1331, 1671
BstDSI CCRYGG 1 cut(s) 499
BstF5I GGATG 2 cut(s) 259, 429
BstFNI CGCG 2 cut(s) 324, 944
BstHHI GCGC 4 cut(s) 324, 704, 944, 946
BstKTI GATC 7 cut(s) 246, 655, 1202, 1324, 1369, 1426, 1561
BstMAI GTCTC 5 cut(s) 127, 1063, 1327, 1348, 1595
BstMBI GATC 7 cut(s) 243, 652, 1199, 1321, 1366, 1423, 1558
BstMCI CGRYCG 1 cut(s) 484
BstMWI GCNNNNNNNGC 5 cut(s) 100, 389, 467, 522, 970
BstNI CCWGG 3 cut(s) 241, 489, 867
BstNSI RCATGY 2 cut(s) 1556, 1693
BstSCI CCNGG 3 cut(s) 239, 487, 865
BstSFI CTRYAG 2 cut(s) 517, 876
BstSNI TACGTA 1 cut(s) 1518
BstUI CGCG 2 cut(s) 324, 944
BstV1I GCAGC 7 cut(s) 262, 352, 454, 457, 597, 958, 1705
BstV2I GAAGAC 3 cut(s) 427, 903, 1692
BstX2I RGATCY 3 cut(s) 652, 1321, 1423
BstXI CCANNNNNNTGG 4 cut(s) 271, 567, 763, 1324
BstYI RGATCY 3 cut(s) 652, 1321, 1423
Bsu15I ATCGAT 1 cut(s) 1561
BsuRI GGCC 4 cut(s) 392, 592, 964, 1453
BsuTUI ATCGAT 1 cut(s) 1561
BtgI CCRYGG 1 cut(s) 499
BtrI CACGTC 1 cut(s) 1737
BtsCI GGATG 2 cut(s) 259, 429
BtsI GCAGTG 2 cut(s) 345, 456
BtsIMutI CAGTG 6 cut(s) 264, 345, 385, 402, 456, 771
Cac8I GCNNGC 5 cut(s) 84, 615, 944, 975, 1660
CfoI GCGC 4 cut(s) 324, 704, 944, 946
Cfr13I GGNCC 3 cut(s) 312, 391, 1327
ClaI ATCGAT 1 cut(s) 1561
Csp6I GTAC 4 cut(s) 492, 992, 1047, 1081
CviQI GTAC 4 cut(s) 492, 992, 1047, 1081
DdeI CTNAG 5 cut(s) 351, 774, 1001, 1331, 1671
DpnI GATC 7 cut(s) 245, 654, 1201, 1323, 1368, 1425, 1560
DpnII GATC 7 cut(s) 243, 652, 1199, 1321, 1366, 1423, 1558
DraI TTTAAA 2 cut(s) 205, 916
DriI GACNNNNNGTC 1 cut(s) 1544
Eam1104I CTCTTC 4 cut(s) 19, 28, 1346, 1638
Eam1105I GACNNNNNGTC 1 cut(s) 1544
EarI CTCTTC 4 cut(s) 19, 28, 1346, 1638
EciI GGCGGA 1 cut(s) 723
Ecl136II GAGCTC 1 cut(s) 252
Eco105I TACGTA 1 cut(s) 1518
Eco130I CCWWGG 1 cut(s) 857
Eco147I AGGCCT 1 cut(s) 964
Eco24I GRGCYC 2 cut(s) 254, 866
Eco47I GGWCC 2 cut(s) 312, 1327
Eco53kI GAGCTC 1 cut(s) 252
Eco57I CTGAAG 2 cut(s) 369, 588
EcoICRI GAGCTC 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 391
EcoRI GAATTC 1 cut(s) 850
EcoRII CCWGG 3 cut(s) 239, 487, 865
EcoT14I CCWWGG 1 cut(s) 857
EcoT22I ATGCAT 1 cut(s) 429
EcoT38I GRGCYC 2 cut(s) 254, 866
ErhI CCWWGG 1 cut(s) 857
Esp3I CGTCTC 2 cut(s) 127, 1063
FalI AAGNNNNNCTT 2 cut(s) 952, 984
FaqI GGGAC 2 cut(s) 1115, 1718
FauI CCCGC 2 cut(s) 472, 1433
Fnu4HI GCNGC 7 cut(s) 276, 341, 468, 471, 611, 947, 1694
FokI GGATG 2 cut(s) 246, 436
FriOI GRGCYC 2 cut(s) 254, 866
Fsp4HI GCNGC 7 cut(s) 276, 341, 468, 471, 611, 947, 1694
FspBI CTAG 8 cut(s) 141, 450, 534, 800, 816, 821, 978, 1421
GlaI GCGC 4 cut(s) 323, 703, 943, 945
GluI GCNGC 7 cut(s) 276, 341, 468, 471, 611, 947, 1694
HaeIII GGCC 4 cut(s) 392, 592, 964, 1453
HapII CCGG 2 cut(s) 197, 1473
HhaI GCGC 4 cut(s) 324, 704, 944, 946
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 4 cut(s) 322, 702, 942, 944
HinP1I GCGC 4 cut(s) 322, 702, 942, 944
HincII GTYRAC 1 cut(s) 1018
HindII GTYRAC 1 cut(s) 1018
HpaI GTTAAC 1 cut(s) 1018
HpaII CCGG 2 cut(s) 197, 1473
HphI GGTGA 3 cut(s) 728, 848, 1354
Hpy166II GTNNAC 6 cut(s) 1018, 1047, 1083, 1120, 1300, 1435
Hpy8I GTNNAC 6 cut(s) 1018, 1047, 1083, 1120, 1300, 1435
HpyCH4III ACNGT 3 cut(s) 268, 380, 433
HpyCH4IV ACGT 4 cut(s) 30, 1517, 1595, 1736
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 389, 467, 522, 970
HpyF3I CTNAG 5 cut(s) 351, 774, 1001, 1331, 1671
HpySE526I ACGT 4 cut(s) 30, 1517, 1595, 1736
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 4 cut(s) 322, 702, 942, 944
Kpn2I TCCGGA 2 cut(s) 196, 1472
KpnI GGTACC 1 cut(s) 495
KspAI GTTAAC 1 cut(s) 1018
Kzo9I GATC 7 cut(s) 243, 652, 1199, 1321, 1366, 1423, 1558
LguI GCTCTTC 1 cut(s) 28
LmnI GCTCC 3 cut(s) 466, 861, 1005
Lsp1109I GCAGC 7 cut(s) 262, 352, 454, 457, 597, 958, 1705
LweI GCATC 5 cut(s) 81, 414, 1195, 1329, 1374
MaeI CTAG 8 cut(s) 141, 450, 534, 800, 816, 821, 978, 1421
MaeII ACGT 4 cut(s) 30, 1517, 1595, 1736
MaeIII GTNAC 4 cut(s) 1231, 1286, 1360, 1635
MalI GATC 7 cut(s) 245, 654, 1201, 1323, 1368, 1425, 1560
MboI GATC 7 cut(s) 243, 652, 1199, 1321, 1366, 1423, 1558
MflI RGATCY 3 cut(s) 652, 1321, 1423
MhlI GDGCHC 3 cut(s) 254, 619, 866
MluCI AATT 7 cut(s) 67, 728, 850, 1039, 1137, 1525, 1605
MlyI GAGTC 1 cut(s) 1350
MmeI TCCRAC 3 cut(s) 51, 60, 680
MnlI CCTC 8 cut(s) 84, 162, 169, 382, 515, 582, 712, 918
Mph1103I ATGCAT 1 cut(s) 429
MroI TCCGGA 2 cut(s) 196, 1472
MroXI GAANNNNTTC 1 cut(s) 1465
MseI TTAA 8 cut(s) 51, 204, 744, 750, 806, 915, 1017, 1505
MslI CAYNNNNRTG 3 cut(s) 565, 694, 1295
MspA1I CMGCKG 2 cut(s) 467, 1696
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 2 cut(s) 197, 1473
MspR9I CCNGG 3 cut(s) 241, 489, 867
Mva1269I GAATGC 2 cut(s) 1392, 1654
MvaI CCWGG 3 cut(s) 241, 489, 867
MvnI CGCG 2 cut(s) 324, 944
MwoI GCNNNNNNNGC 5 cut(s) 100, 389, 467, 522, 970
NdeII GATC 7 cut(s) 243, 652, 1199, 1321, 1366, 1423, 1558
NlaIV GGNNCC 4 cut(s) 493, 654, 863, 1007
NmuCI GTSAC 1 cut(s) 1360
NsiI ATGCAT 1 cut(s) 429
NspI RCATGY 2 cut(s) 1556, 1693
NspV TTCGAA 1 cut(s) 1152
OliI CACNNNNGTG 1 cut(s) 565
PasI CCCWGGG 1 cut(s) 866
PauI GCGCGC 1 cut(s) 942
PceI AGGCCT 1 cut(s) 964
PciSI GCTCTTC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 481
PctI GAATGC 2 cut(s) 1392, 1654
PdmI GAANNNNTTC 1 cut(s) 1465
PfoI TCCNGGA 1 cut(s) 239
PkrI GCNGC 7 cut(s) 277, 342, 469, 472, 612, 948, 1695
PleI GAGTC 1 cut(s) 1350
PpsI GAGTC 1 cut(s) 1350
Ppu21I YACGTR 2 cut(s) 1518, 1596
Psp124BI GAGCTC 1 cut(s) 254
Psp1406I AACGTT 1 cut(s) 30
Psp6I CCWGG 3 cut(s) 239, 487, 865
PspGI CCWGG 3 cut(s) 239, 487, 865
PspN4I GGNNCC 4 cut(s) 493, 654, 863, 1007
PspPI GGNCC 3 cut(s) 312, 391, 1327
PsuI RGATCY 3 cut(s) 652, 1321, 1423
PteI GCGCGC 1 cut(s) 942
PvuII CAGCTG 1 cut(s) 1696
RsaI GTAC 4 cut(s) 493, 993, 1048, 1082
RsaNI GTAC 4 cut(s) 492, 992, 1047, 1081
RseI CAYNNNNRTG 3 cut(s) 565, 694, 1295
SacI GAGCTC 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 28
SaqAI TTAA 8 cut(s) 51, 204, 744, 750, 806, 915, 1017, 1505
SatI GCNGC 7 cut(s) 276, 341, 468, 471, 611, 947, 1694
Sau3AI GATC 7 cut(s) 243, 652, 1199, 1321, 1366, 1423, 1558
Sau96I GGNCC 3 cut(s) 312, 391, 1327
ScaI AGTACT 1 cut(s) 993
SchI GAGTC 1 cut(s) 1350
ScrFI CCNGG 3 cut(s) 241, 489, 867
SduI GDGCHC 3 cut(s) 254, 619, 866
SfaNI GCATC 5 cut(s) 81, 414, 1195, 1329, 1374
SfcI CTRYAG 2 cut(s) 517, 876
SfuI TTCGAA 1 cut(s) 1152
SinI GGWCC 2 cut(s) 312, 1327
SmiMI CAYNNNNRTG 3 cut(s) 565, 694, 1295
SmlI CTYRAG 2 cut(s) 749, 1714
SmoI CTYRAG 2 cut(s) 749, 1714
SnaBI TACGTA 1 cut(s) 1518
SpeI ACTAGT 1 cut(s) 815
Sse9I AATT 7 cut(s) 67, 728, 850, 1039, 1137, 1525, 1605
SseBI AGGCCT 1 cut(s) 964
SsiI CCGC 6 cut(s) 465, 480, 734, 834, 1440, 1556
SspMI CTAG 8 cut(s) 141, 450, 534, 800, 816, 821, 978, 1421
SstI GAGCTC 1 cut(s) 254
StuI AGGCCT 1 cut(s) 964
StyD4I CCNGG 3 cut(s) 239, 487, 865
StyI CCWWGG 1 cut(s) 857
TaaI ACNGT 3 cut(s) 268, 380, 433
TaiI ACGT 4 cut(s) 33, 1520, 1598, 1739
TaqI TCGA 3 cut(s) 575, 1152, 1561
TasI AATT 7 cut(s) 67, 728, 850, 1039, 1137, 1525, 1605
TatI WGTACW 3 cut(s) 991, 1046, 1080
Tru1I TTAA 8 cut(s) 51, 204, 744, 750, 806, 915, 1017, 1505
Tru9I TTAA 8 cut(s) 51, 204, 744, 750, 806, 915, 1017, 1505
TscAI CASTG 6 cut(s) 271, 352, 385, 409, 463, 778
TseFI GTSAC 1 cut(s) 1360
TseI GCWGC 7 cut(s) 275, 340, 467, 470, 610, 946, 1693
Tsp45I GTSAC 1 cut(s) 1360
TspGWI ACGGA 1 cut(s) 488
TspRI CASTG 6 cut(s) 271, 352, 385, 409, 463, 778
Vha464I CTTAAG 1 cut(s) 749
VpaK11BI GGWCC 2 cut(s) 312, 1327
XapI RAATTY 3 cut(s) 850, 1039, 1525
XbaI TCTAGA 2 cut(s) 820, 1420
XceI RCATGY 2 cut(s) 1556, 1693
XcmI CCANNNNNNNNNTGG 1 cut(s) 564
XmnI GAANNNNTTC 1 cut(s) 1465
XspI CTAG 8 cut(s) 141, 450, 534, 800, 816, 821, 978, 1421
ZrmI AGTACT 1 cut(s) 993
Zsp2I ATGCAT 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.