pycom12g23260

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001068
Physical Location & Seq
Forward (+)
23906102 .. 23909069
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g23260.1

Sequence Viewer

Length: 903 bp
ATGTGTTTTAACAGTATACATAGAACCCTCCACCAAGTTGTCACCAGAAAAATAAAAAAGAAAACCCCCTCAGGAAGTTTTACTGTATCACAAAAAGTTGAAGATGAAGAAGAAAAGGTGGGAACTTTCGAATTCCATTTCCATGTCTATAAAACCTCACAAGGCAATGCACAGAGTATAAGTGCTAGTTCTGCAATGGCTTACGTTCCACCACACAGGCGGCACTTGAAGGAATCGGAGAGGCCATTGCTGACTCCAGAGCTGCTTGCTCCTCAATACAAGAAAAACTTAAGTATCAAGCCATATAATAAGTCTAATGTGGAATCGAATGGAGAAATAGTTTATGCAGACCATTCTACATTTAGATGGTGCGCCATCGCTTTGGATGACGAGAACCAGTTTCCATCTTCTGTTAATCTTGAGCCCATTACCTTGGAGTCAGCTGAGCTGAATTATGGAAAAAATCGTGTAGCTCTGATCAATACTAGTCTAATTAATGAAGGAGGTGAGGTGAAATGGAATTTGCCAAGGAGCCCTTGGGAATCTATAACAGAAAATGTGCTGGAAGACTTACTTTCGACATTTAAACACGTGAGGAATGAAATGAAGTCTGCAAAGCTCAAAGAAGTATATCCAACCTTGGTTGCCAGAGTAGGGAAAGTACTCTTTCGCAGGAGCCCTTCAGTTAACATGGAATCCATCAGAAAAAAATTGTGTACTGAAATCTCGAAACAATGGAACCGATTATTTTACATAAACATTCCTGTTTCATATAAGGAAAAGATTGTGAACGAAGTTGTCCCCAAAATTGGAGTTGATTTCGAAGAGGAGGAAGATACATACGAAGTACAGTTGTCTGATTCTACGAGTCCAGATTCAACTCTCTTCTGCAGATGTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

34.45

Weight (kDa)

8.16

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7903 PF25475 109 - 300 2.5e-68 Domain of unknown function (DUF7903)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 16
AciI CCGC 1 cut(s) 220
AcsI RAATTY 2 cut(s) 131, 520
AcuI CTGAAG 1 cut(s) 666
AcvI CACGTG 1 cut(s) 592
AfaI GTAC 3 cut(s) 663, 718, 849
AfiI CCNNNNNNNGG 2 cut(s) 654, 809
AflII CTTAAG 1 cut(s) 289
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 3 cut(s) 101, 229, 879
AhlI ACTAGT 1 cut(s) 485
AluBI AGCT 5 cut(s) 262, 443, 448, 473, 619
AluI AGCT 5 cut(s) 262, 443, 448, 473, 619
AoxI GGCC 1 cut(s) 242
ApeKI GCWGC 1 cut(s) 262
ApoI RAATTY 2 cut(s) 131, 520
AseI ATTAAT 1 cut(s) 495
AspLEI GCGC 1 cut(s) 374
AsuHPI GGTGA 3 cut(s) 34, 518, 523
AsuII TTCGAA 2 cut(s) 129, 822
AxyI CCTNAGG 1 cut(s) 70
BaeI ACNNNNGTAYC 2 cut(s) 277, 310
BanII GRGCYC 3 cut(s) 426, 536, 680
BbrPI CACGTG 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 573
BbvI GCAGC 1 cut(s) 249
BccI CCATC 4 cut(s) 360, 383, 412, 707
BclI TGATCA 1 cut(s) 477
BcuI ACTAGT 1 cut(s) 485
BfaI CTAG 2 cut(s) 186, 486
BfmI CTRYAG 1 cut(s) 889
BfrI CTTAAG 1 cut(s) 289
BisI GCNGC 2 cut(s) 221, 263
BlpI GCTNAGC 1 cut(s) 444
BlsI GCNGC 2 cut(s) 222, 264
BmcAI AGTACT 1 cut(s) 663
BmiI GGNNCC 3 cut(s) 533, 677, 740
BpiI GAAGAC 1 cut(s) 573
BpmI CTGGAG 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 444
Bpu14I TTCGAA 2 cut(s) 129, 822
BpuEI CTTGAG 1 cut(s) 440
BsaAI YACGTR 1 cut(s) 592
BsaJI CCNNGG 4 cut(s) 432, 527, 536, 639
BsaXI ACNNNNNCTCC 4 cut(s) 324, 354, 495, 525
Bsc4I CCNNNNNNNGG 2 cut(s) 654, 809
Bse1I ACTGG 1 cut(s) 397
Bse21I CCTNAGG 1 cut(s) 70
Bse3DI GCAATG 3 cut(s) 172, 201, 245
BseDI CCNNGG 4 cut(s) 432, 527, 536, 639
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 2 cut(s) 654, 809
BseMI GCAATG 3 cut(s) 172, 201, 245
BseMII CTCAG 2 cut(s) 84, 435
BseNI ACTGG 1 cut(s) 397
BseRI GAGGAG 2 cut(s) 261, 842
BseXI GCAGC 1 cut(s) 249
BshFI GGCC 1 cut(s) 244
BslFI GGGAC 1 cut(s) 785
BslI CCNNNNNNNGG 2 cut(s) 654, 809
BsmFI GGGAC 1 cut(s) 785
BsnI GGCC 1 cut(s) 244
Bsp119I TTCGAA 2 cut(s) 129, 822
Bsp1286I GDGCHC 3 cut(s) 426, 536, 680
Bsp143I GATC 1 cut(s) 477
Bsp1720I GCTNAGC 1 cut(s) 444
BspACI CCGC 1 cut(s) 220
BspANI GGCC 1 cut(s) 244
BspCNI CTCAG 2 cut(s) 83, 436
BspLI GGNNCC 3 cut(s) 533, 677, 740
BspMAI CTGCAG 1 cut(s) 893
BspT104I TTCGAA 2 cut(s) 129, 822
BspTI CTTAAG 1 cut(s) 289
BsrDI GCAATG 3 cut(s) 172, 201, 245
BsrI ACTGG 1 cut(s) 397
BssECI CCNNGG 4 cut(s) 432, 527, 536, 639
BssMI GATC 1 cut(s) 477
BssNAI GTATAC 1 cut(s) 17
BssT1I CCWWGG 4 cut(s) 432, 527, 536, 639
Bst1107I GTATAC 1 cut(s) 17
Bst4CI ACNGT 3 cut(s) 14, 85, 852
Bst6I CTCTTC 2 cut(s) 819, 890
BstAFI CTTAAG 1 cut(s) 289
BstBAI YACGTR 1 cut(s) 592
BstBI TTCGAA 2 cut(s) 129, 822
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 2 cut(s) 70, 444
BstF5I GGATG 1 cut(s) 391
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 1 cut(s) 480
BstMBI GATC 1 cut(s) 477
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstSFI CTRYAG 1 cut(s) 889
BstV1I GCAGC 1 cut(s) 249
BstV2I GAAGAC 1 cut(s) 573
BstXI CCANNNNNNTGG 2 cut(s) 382, 433
BstZ17I GTATAC 1 cut(s) 17
Bsu36I CCTNAGG 1 cut(s) 70
BsuRI GGCC 1 cut(s) 244
BtgZI GCGATG 1 cut(s) 361
BtsCI GGATG 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 267
CfoI GCGC 1 cut(s) 374
Csp6I GTAC 3 cut(s) 662, 717, 848
CviAII CATG 2 cut(s) 143, 691
CviQI GTAC 3 cut(s) 662, 717, 848
DdeI CTNAG 2 cut(s) 70, 444
DpnI GATC 1 cut(s) 479
DpnII GATC 1 cut(s) 477
DraI TTTAAA 1 cut(s) 586
Eam1104I CTCTTC 2 cut(s) 819, 890
EarI CTCTTC 2 cut(s) 819, 890
Eco130I CCWWGG 4 cut(s) 432, 527, 536, 639
Eco24I GRGCYC 3 cut(s) 426, 536, 680
Eco57I CTGAAG 1 cut(s) 666
Eco72I CACGTG 1 cut(s) 592
Eco81I CCTNAGG 1 cut(s) 70
EcoRI GAATTC 1 cut(s) 131
EcoT14I CCWWGG 4 cut(s) 432, 527, 536, 639
EcoT38I GRGCYC 3 cut(s) 426, 536, 680
ErhI CCWWGG 4 cut(s) 432, 527, 536, 639
FaeI CATG 2 cut(s) 146, 694
FalI AAGNNNNNCTT 6 cut(s) 272, 304, 520, 552, 558, 590
FaqI GGGAC 1 cut(s) 785
FatI CATG 2 cut(s) 142, 690
FbaI TGATCA 1 cut(s) 477
FblI GTMKAC 1 cut(s) 16
Fnu4HI GCNGC 2 cut(s) 221, 263
FokI GGATG 1 cut(s) 398
FriOI GRGCYC 3 cut(s) 426, 536, 680
Fsp4HI GCNGC 2 cut(s) 221, 263
FspBI CTAG 2 cut(s) 186, 486
GlaI GCGC 1 cut(s) 373
GluI GCNGC 2 cut(s) 221, 263
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 1 cut(s) 244
HhaI GCGC 1 cut(s) 374
Hin1II CATG 2 cut(s) 146, 694
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HinfI GANTC 9 cut(s) 233, 253, 323, 437, 542, 695, 860, 868, 875
HpaI GTTAAC 1 cut(s) 688
HphI GGTGA 3 cut(s) 34, 518, 523
Hpy166II GTNNAC 4 cut(s) 17, 688, 717, 790
Hpy188I TCNGA 4 cut(s) 238, 477, 704, 859
Hpy188III TCNNGA 5 cut(s) 72, 257, 419, 727, 872
Hpy8I GTNNAC 4 cut(s) 17, 688, 717, 790
HpyAV CCTTC 3 cut(s) 223, 494, 690
HpyCH4III ACNGT 3 cut(s) 14, 85, 852
HpyCH4IV ACGT 2 cut(s) 204, 591
HpyCH4V TGCA 5 cut(s) 170, 194, 347, 614, 891
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpyF3I CTNAG 2 cut(s) 70, 444
HpySE526I ACGT 2 cut(s) 204, 591
Hsp92II CATG 2 cut(s) 146, 694
HspAI GCGC 1 cut(s) 372
Ksp22I TGATCA 1 cut(s) 477
KspAI GTTAAC 1 cut(s) 688
Kzo9I GATC 1 cut(s) 477
LmnI GCTCC 3 cut(s) 274, 531, 675
Lsp1109I GCAGC 1 cut(s) 249
MaeI CTAG 2 cut(s) 186, 486
MaeII ACGT 2 cut(s) 204, 591
MaeIII GTNAC 1 cut(s) 40
MalI GATC 1 cut(s) 479
MboI GATC 1 cut(s) 477
MboII GAAGA 8 cut(s) 113, 119, 122, 399, 578, 836, 845, 877
MhlI GDGCHC 3 cut(s) 426, 536, 680
MluCI AATT 6 cut(s) 131, 451, 492, 520, 710, 807
MlyI GAGTC 3 cut(s) 247, 446, 877
MmeI TCCRAC 1 cut(s) 659
MseI TTAA 6 cut(s) 9, 290, 414, 495, 585, 687
MslI CAYNNNNRTG 2 cut(s) 141, 364
MspA1I CMGCKG 1 cut(s) 443
MspCI CTTAAG 1 cut(s) 289
MwoI GCNNNNNNNGC 1 cut(s) 191
NdeII GATC 1 cut(s) 477
NlaIII CATG 2 cut(s) 146, 694
NlaIV GGNNCC 3 cut(s) 533, 677, 740
NmuCI GTSAC 1 cut(s) 40
NspV TTCGAA 2 cut(s) 129, 822
PfeI GAWTC 6 cut(s) 233, 323, 542, 695, 860, 875
PkrI GCNGC 2 cut(s) 222, 264
PleI GAGTC 3 cut(s) 247, 445, 876
PmaCI CACGTG 1 cut(s) 592
PmlI CACGTG 1 cut(s) 592
PpsI GAGTC 3 cut(s) 247, 445, 876
Ppu21I YACGTR 1 cut(s) 592
PshBI ATTAAT 1 cut(s) 495
PspCI CACGTG 1 cut(s) 592
PspN4I GGNNCC 3 cut(s) 533, 677, 740
PstI CTGCAG 1 cut(s) 893
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 3 cut(s) 663, 718, 849
RsaNI GTAC 3 cut(s) 662, 717, 848
RseI CAYNNNNRTG 2 cut(s) 141, 364
SaqAI TTAA 6 cut(s) 9, 290, 414, 495, 585, 687
SatI GCNGC 2 cut(s) 221, 263
Sau3AI GATC 1 cut(s) 477
ScaI AGTACT 1 cut(s) 663
SchI GAGTC 3 cut(s) 247, 446, 877
SduI GDGCHC 3 cut(s) 426, 536, 680
SfcI CTRYAG 1 cut(s) 889
SfuI TTCGAA 2 cut(s) 129, 822
SmiMI CAYNNNNRTG 2 cut(s) 141, 364
SmlI CTYRAG 2 cut(s) 289, 419
SmoI CTYRAG 2 cut(s) 289, 419
SpeI ACTAGT 1 cut(s) 485
Sse9I AATT 6 cut(s) 131, 451, 492, 520, 710, 807
SsiI CCGC 1 cut(s) 220
SspMI CTAG 2 cut(s) 186, 486
StyI CCWWGG 4 cut(s) 432, 527, 536, 639
TaaI ACNGT 3 cut(s) 14, 85, 852
TaiI ACGT 2 cut(s) 207, 594
TaqI TCGA 5 cut(s) 129, 326, 578, 728, 822
TasI AATT 6 cut(s) 131, 451, 492, 520, 710, 807
TatI WGTACW 3 cut(s) 661, 716, 847
TauI GCSGC 1 cut(s) 223
TfiI GAWTC 6 cut(s) 233, 323, 542, 695, 860, 875
Tru1I TTAA 6 cut(s) 9, 290, 414, 495, 585, 687
Tru9I TTAA 6 cut(s) 9, 290, 414, 495, 585, 687
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 1 cut(s) 262
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 5 cut(s) 120, 513, 615, 620, 759
Vha464I CTTAAG 1 cut(s) 289
VspI ATTAAT 1 cut(s) 495
XapI RAATTY 2 cut(s) 131, 520
XcmI CCANNNNNNNNNTGG 1 cut(s) 534
XmiI GTMKAC 1 cut(s) 16
XspI CTAG 2 cut(s) 186, 486
ZrmI AGTACT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.